Research Article
BibTex RIS Cite

Türkiye’de Yetiştirilen Farklı Yonca (Medicago sativa L.) Çeşitlerinin Antioksidan Aktiviteleri ve Toplam Fenolik Madde İçeriği

Year 2021, Volume: 31 Issue: 4, 995 - 1000, 31.12.2021

Hakime Hülya Orak Magdalena Karamać Adnan Orak Hazım Serkan Tenikecier Ryszard Amarowicz

https://doi.org/10.29133/yyutbd.946487

Abstract

Bu çalışmada Türkiye'de yetiştirilen 15 yonca çeşidi, antioksidan potansiyellerini belirlemek ve çeşitler arasındaki farklılıkları karşılaştırmak amacıyla araştırılmıştır. Yonca tohumu metanol ekstraktlarında toplam fenolik madde içeriği, DPPH•, ABTS•+ giderim ve FRAP aktiviteleri belirlenmiştir. Ekstrelerin TP değerleri 37.03 mg GAE/g ile 54.04 mg GAE/g arasında bulunmuştur. TEAC değerleri 0.100 mmol Trolox/g ile 0.158 mmol Trolox/g arasında değişmiştir. FRAP aktivitesi 389.90 µmol Fe2+/g'den 791.02 µmol Fe2+/g aralığında değişim göstermiştir. Sonuçlar, incelenen karakterler açısından yonca çeşitleri arasındaki farklılıkların istatistiksel olarak önemli (p < 0.01) olduğunu göstermektedir.

Keywords

Alfalfa Medicago sativa L. Fenolik içerik Tohum Çeşit Antioksidan aktivite

References

  • Abbruscato, P., Tosi, S., Crispino, L., Biazzi, E., Menin, B., Picco, A. M., Pecetti, L., Avato, P., & Tava, A. (2014) Triterpenoid glycosides from Medicago sativa as antifungal agents against Pyricularia oryzae. Journal of Agricultural and Food Chemistry 62:11030-11036.
  • Aguilera, Y., Estrella, I., Benitez, V., Esteban, R. M. & Martín-Cabrejas, M. A. (2011). Bioactive phenolic compounds and functional properties of dehydrated bean flours. Food Research International 44, 774-780.
  • Al-Dosari, M. S. (2012). In vitro and in vivo antioxidant activity of Alfalfa (Medicago sativa L.) on carbon tetrachloride intoxicated rats. The American Journal of Chinese Medicine 40(4), 779-793.
  • Amarowicz, R., & Weidner, S. (2009). Biological activity of grapevine phenolic compounds. In K. A. Roubelakis Angelakis (Eds.), Grapevine molecular physiology and biotechnology (2nd ed., pp. 389–405). Heraklion, Springer Science + Business Media B.V
  • Asadi-Samani, M., Rafieian-Kopaei, M., Lorigooini, Z., & Shirzad, H. (2018). A screening to determine total phenol and flavonoid content of some Iran’s medicinal plants grown in Chaharmahal va Bakhtyari province. Indian Journal of Natural Products and Resources 9(4), 296-302.
  • Barnes, J., Anderson, L. A., & Philipson, J. D. (2007). Herbal Medicines, third edition. London: Pharmaceutical Press.
  • Barreira, J. C. M., Visnevschi-Necrasov, T., Nunes, E., Cunha, S., C., Pereira, G., & Oliveira, M. B. P. P. (2015). Medicago spp. as potential sources of bioactive isoflavones: characterization according to phylogenetic and phenologic factors. Phytochemistry 116, 230-8.
  • Benzie, I., F., F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry 239, 70-76.
  • Bora, K., S., & Sharma, A., (2011) Phytochemical and pharmacological potential of Medicago sativa: A review. Pharmaceutical Biology 49(2), 211-220.
  • Brand-Williams, W., Cuvelier, M., E., & Berset, C. (1995). Use of a free-radical method to evaluate antioxidant activity. LWT Food Science and Technology 28, 25 Cornara, L., Xiao J., B., & Burlando, B. (2016). Therapeutic potential of temperate forage legumes: 30. Cornara, L., Xiao J., B., & Burlando, B. (2016). Therapeutic potential of temperate forage legumes: A review. Critical Reviews in Food Science and Nutrition 56, 149-161.
  • Gatouillat, G., Magid, A., A., Bertin, E., Okiemy-Akeli, M. G., Morjani, H., Lavaud, C., & Madoulet, C. (2014). Cytotoxicity and apoptosis induced by alfalfa (Medicago sativa L.) leaf extracts in sensitive and multidrug-resistant tumor cells. Nutrition and Cancer 66, 483-491.
  • Gawel, E., Grzelak, M., Janyszek, M. (2017). Lucerne (Medicago sativa L.) in the human dietcase reports and short reports. Journal of Herbal Medicine 10, 8-16.
  • Gaweł, E., (2012). Chemical compositions of lucerne leaf extract (EFL) and its applications as a phytobiotic in human nutrition. Acta Scientiarum Polonorum Technologia Alimentaria 11, 303-309.
  • Gomathi, R., Banu, S., & Usha, K. (2016). Phytochemical analysis and free radical scavenging potential of Medicago sativa Linn seeds. International Research Journal of Pharmacy 7(6), 71-76.
  • Hanif, M. A., Al-Maskari, A. Y., Al-Sabahi, J. N., Al-Hdhrami, I., Khan, M. M., Al-Azkawi, A., & Hussain, A. I. (2015). Chemical characterisation of bioactive compounds in Medicago sativa growing in the desert of Oman. National Product Research 29(24), 2332-2335.
  • Hall, D. (1981). The Herb Tea Book. Connecticut: Keats Publishing, 234-239. Hong, Y. H., Chao, W. W., Chen, M. L., & Lin, B. F. (2009) Ethyl acetate extracts of alfalfa (Medicago sativa L.) sprouts inhibit lipopolysaccharide induced inflammation in vitro and in vivo. Journal of Biomedical Science 16, 64-75.
  • Kabtni, S., Sdouga, D., Bettaib Rebey, I., Save, M., Trifi-Farah, N., Fauconnier, M. L., & Marghali, S. (2020). Influence of climate variation on phenolic composition and antioxidant capacity of Medicago minima populations. Scientific Reports 10(1), 8293.
  • Karamać, M., Amarowicz, R., Weidner, S., & Shahidi, F. (2004). Antioxidant activity of phenolic fractions of white bean (Phaseolus vulgaris L.). Journal of Food Lipids 11, 165-177.
  • Karamać M., Orak H. H., Amarowicz R., Orak A., & Piekoszewski W. (2018). Phenolic contents and antioxidant capacities of wild and cultivated white lupin (Lupinus albus L.) seeds. Food Chemistry, 258, 1-7.
  • Karamać, M., Gai, F., Peiretti, P. G. (2020) Effect of the growth stage of false flax (Camelina sativa L.) on the phenolic compound content and antioxidant potential of the aerial part of the plant. Polish Journal of Food and Nutrition Sciences, 2020, 70, 189–198.
  • Khaleel, A. E. G., Mohamed, Z., El-Maraghy, S. A., Hifnawy, M. S., & Abdel-Sattar, E. (2005). Study of hypocholesterolemic and antiatherosclerotic Medicago sativa L.: A review 219 © 2011 Informa Healthcare USA, Inc. properties of Medicago sativa L. cultivated in Egypt. Yaowu Shipin Fenxi, 13, 212-218.
  • Krakowska, A., Rafińska, K., Walczak, J., Kowalkowski, T., & Buszewski, B. (2017). Comparison of various extraction techniques of Medicago sativa: yield, antioxidant activity, and content of phytochemical constituents. Journal of AOAC International 100, 1-13.
  • Ma, Q. G., Li, T., & Wei, R. R, (2016). Characterization of chalcones from Medicago sativa L. and their hypolipidemic and antiangiogenic activities. Journal of Agricultural and Food Chemistry. 64, 8138- 8145.
  • Martínez, R., Kapravelou, G., Porres, J. M., Melesio, A. M., Heras, L., Cantarero, S., Gribble, F. M., Parker, H., Aranda, P., & López-Jurado, M. (2016). Medicago sativa L. a functional food to relieve hypertension and metabolic disorders in a spontaneously hypertensive rat model. Journal of Functional Foods 26, 470-484.
  • Molgaard, J., Von, S. H., & Olsson, A. G. (1987). Alfalfa seeds lower low-density lipoprotein cholesterol and apolipoprotein B concentrations in patients with type-II hyperlipoproteinemia. Atherosclerosis 65, 173-179.
  • Orak, H. H. (2019). Investigation of Total phenolic contents and antioxidant potentials of some vetch genotypes (Vicia sp.) grown in Turkey. Fresenius Environmental Bulletin 28, 9526-9531.
  • Orak, H. H., Karamać, M., Orak, A., & Amarowicz, R. (2016). Antioxidant potential and phenolic compounds of some widely consumed Turkish white bean (Phaseolus vulgaris L.) varieties. Polish Journal of Food and Nutrition Sciences 66, 253-260.
  • Orak, H. H., Karamać, M., & Amarowicz, R. (2015). Antioxidant Activity of Phenolic Compounds of Red Bean (Phaseolus vulgaris L.). Oxidation Communications 38, 67-76.
  • Orak, H. H., Karamać, M., Orak, A., Amarowicz, R., & Janiak, M. A. (2018). Phenolics Content and Antioxidant Capacity of Mung Bean (Vigna radiata L.) Seed. Yuzuncu Yıl University Journal of Agricultural Sciences 28, 199-207.
  • Rafińska, K., Pomastowski, P., Wrona, O., Górecki, R., & Buszewski, B. (2017). Medicago sativa as a source of secondary metabolites for agriculture and pharmaceutical industry Phytochemistry Letters 20, 520-539.
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine 26,1231-1237.

Antioxidant Activity and Total Phenolic Contents of Different Alfalfa (Medicago sativa L.) Varieties Grown in Turkey

Year 2021, Volume: 31 Issue: 4, 995 - 1000, 31.12.2021

Hakime Hülya Orak Magdalena Karamać Adnan Orak Hazım Serkan Tenikecier Ryszard Amarowicz

https://doi.org/10.29133/yyutbd.946487

Abstract

Fifteen alfalfa varieties cultivated in Turkey were researched to compare the antioxidant potential of their seeds and find differences between the varieties. The total phenolic (TP) content, DPPH•, and ABTS•+ scavenging activities, and ferric-reducing antioxidant power (FRAP) of alfalfa seed methanol extracts were determined. TP contents of extracts were found between 37.03 and 54.04 mg GAE/g. ABTS assay results ranged between 0.100 and 0.158 mmol Trolox/g extract, and FRAP changed from 389.90 to 791.02 µmol Fe2+/g extract. The results demonstrated that the differences between the alfalfa varieties were significant (p < 0.01) in terms of analyzed characters.

Keywords

Alfalfa Antioxidant activity Medicago sativa L. Phenolic contents Seed Variety

References

  • Abbruscato, P., Tosi, S., Crispino, L., Biazzi, E., Menin, B., Picco, A. M., Pecetti, L., Avato, P., & Tava, A. (2014) Triterpenoid glycosides from Medicago sativa as antifungal agents against Pyricularia oryzae. Journal of Agricultural and Food Chemistry 62:11030-11036.
  • Aguilera, Y., Estrella, I., Benitez, V., Esteban, R. M. & Martín-Cabrejas, M. A. (2011). Bioactive phenolic compounds and functional properties of dehydrated bean flours. Food Research International 44, 774-780.
  • Al-Dosari, M. S. (2012). In vitro and in vivo antioxidant activity of Alfalfa (Medicago sativa L.) on carbon tetrachloride intoxicated rats. The American Journal of Chinese Medicine 40(4), 779-793.
  • Amarowicz, R., & Weidner, S. (2009). Biological activity of grapevine phenolic compounds. In K. A. Roubelakis Angelakis (Eds.), Grapevine molecular physiology and biotechnology (2nd ed., pp. 389–405). Heraklion, Springer Science + Business Media B.V
  • Asadi-Samani, M., Rafieian-Kopaei, M., Lorigooini, Z., & Shirzad, H. (2018). A screening to determine total phenol and flavonoid content of some Iran’s medicinal plants grown in Chaharmahal va Bakhtyari province. Indian Journal of Natural Products and Resources 9(4), 296-302.
  • Barnes, J., Anderson, L. A., & Philipson, J. D. (2007). Herbal Medicines, third edition. London: Pharmaceutical Press.
  • Barreira, J. C. M., Visnevschi-Necrasov, T., Nunes, E., Cunha, S., C., Pereira, G., & Oliveira, M. B. P. P. (2015). Medicago spp. as potential sources of bioactive isoflavones: characterization according to phylogenetic and phenologic factors. Phytochemistry 116, 230-8.
  • Benzie, I., F., F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry 239, 70-76.
  • Bora, K., S., & Sharma, A., (2011) Phytochemical and pharmacological potential of Medicago sativa: A review. Pharmaceutical Biology 49(2), 211-220.
  • Brand-Williams, W., Cuvelier, M., E., & Berset, C. (1995). Use of a free-radical method to evaluate antioxidant activity. LWT Food Science and Technology 28, 25 Cornara, L., Xiao J., B., & Burlando, B. (2016). Therapeutic potential of temperate forage legumes: 30. Cornara, L., Xiao J., B., & Burlando, B. (2016). Therapeutic potential of temperate forage legumes: A review. Critical Reviews in Food Science and Nutrition 56, 149-161.
  • Gatouillat, G., Magid, A., A., Bertin, E., Okiemy-Akeli, M. G., Morjani, H., Lavaud, C., & Madoulet, C. (2014). Cytotoxicity and apoptosis induced by alfalfa (Medicago sativa L.) leaf extracts in sensitive and multidrug-resistant tumor cells. Nutrition and Cancer 66, 483-491.
  • Gawel, E., Grzelak, M., Janyszek, M. (2017). Lucerne (Medicago sativa L.) in the human dietcase reports and short reports. Journal of Herbal Medicine 10, 8-16.
  • Gaweł, E., (2012). Chemical compositions of lucerne leaf extract (EFL) and its applications as a phytobiotic in human nutrition. Acta Scientiarum Polonorum Technologia Alimentaria 11, 303-309.
  • Gomathi, R., Banu, S., & Usha, K. (2016). Phytochemical analysis and free radical scavenging potential of Medicago sativa Linn seeds. International Research Journal of Pharmacy 7(6), 71-76.
  • Hanif, M. A., Al-Maskari, A. Y., Al-Sabahi, J. N., Al-Hdhrami, I., Khan, M. M., Al-Azkawi, A., & Hussain, A. I. (2015). Chemical characterisation of bioactive compounds in Medicago sativa growing in the desert of Oman. National Product Research 29(24), 2332-2335.
  • Hall, D. (1981). The Herb Tea Book. Connecticut: Keats Publishing, 234-239. Hong, Y. H., Chao, W. W., Chen, M. L., & Lin, B. F. (2009) Ethyl acetate extracts of alfalfa (Medicago sativa L.) sprouts inhibit lipopolysaccharide induced inflammation in vitro and in vivo. Journal of Biomedical Science 16, 64-75.
  • Kabtni, S., Sdouga, D., Bettaib Rebey, I., Save, M., Trifi-Farah, N., Fauconnier, M. L., & Marghali, S. (2020). Influence of climate variation on phenolic composition and antioxidant capacity of Medicago minima populations. Scientific Reports 10(1), 8293.
  • Karamać, M., Amarowicz, R., Weidner, S., & Shahidi, F. (2004). Antioxidant activity of phenolic fractions of white bean (Phaseolus vulgaris L.). Journal of Food Lipids 11, 165-177.
  • Karamać M., Orak H. H., Amarowicz R., Orak A., & Piekoszewski W. (2018). Phenolic contents and antioxidant capacities of wild and cultivated white lupin (Lupinus albus L.) seeds. Food Chemistry, 258, 1-7.
  • Karamać, M., Gai, F., Peiretti, P. G. (2020) Effect of the growth stage of false flax (Camelina sativa L.) on the phenolic compound content and antioxidant potential of the aerial part of the plant. Polish Journal of Food and Nutrition Sciences, 2020, 70, 189–198.
  • Khaleel, A. E. G., Mohamed, Z., El-Maraghy, S. A., Hifnawy, M. S., & Abdel-Sattar, E. (2005). Study of hypocholesterolemic and antiatherosclerotic Medicago sativa L.: A review 219 © 2011 Informa Healthcare USA, Inc. properties of Medicago sativa L. cultivated in Egypt. Yaowu Shipin Fenxi, 13, 212-218.
  • Krakowska, A., Rafińska, K., Walczak, J., Kowalkowski, T., & Buszewski, B. (2017). Comparison of various extraction techniques of Medicago sativa: yield, antioxidant activity, and content of phytochemical constituents. Journal of AOAC International 100, 1-13.
  • Ma, Q. G., Li, T., & Wei, R. R, (2016). Characterization of chalcones from Medicago sativa L. and their hypolipidemic and antiangiogenic activities. Journal of Agricultural and Food Chemistry. 64, 8138- 8145.
  • Martínez, R., Kapravelou, G., Porres, J. M., Melesio, A. M., Heras, L., Cantarero, S., Gribble, F. M., Parker, H., Aranda, P., & López-Jurado, M. (2016). Medicago sativa L. a functional food to relieve hypertension and metabolic disorders in a spontaneously hypertensive rat model. Journal of Functional Foods 26, 470-484.
  • Molgaard, J., Von, S. H., & Olsson, A. G. (1987). Alfalfa seeds lower low-density lipoprotein cholesterol and apolipoprotein B concentrations in patients with type-II hyperlipoproteinemia. Atherosclerosis 65, 173-179.
  • Orak, H. H. (2019). Investigation of Total phenolic contents and antioxidant potentials of some vetch genotypes (Vicia sp.) grown in Turkey. Fresenius Environmental Bulletin 28, 9526-9531.
  • Orak, H. H., Karamać, M., Orak, A., & Amarowicz, R. (2016). Antioxidant potential and phenolic compounds of some widely consumed Turkish white bean (Phaseolus vulgaris L.) varieties. Polish Journal of Food and Nutrition Sciences 66, 253-260.
  • Orak, H. H., Karamać, M., & Amarowicz, R. (2015). Antioxidant Activity of Phenolic Compounds of Red Bean (Phaseolus vulgaris L.). Oxidation Communications 38, 67-76.
  • Orak, H. H., Karamać, M., Orak, A., Amarowicz, R., & Janiak, M. A. (2018). Phenolics Content and Antioxidant Capacity of Mung Bean (Vigna radiata L.) Seed. Yuzuncu Yıl University Journal of Agricultural Sciences 28, 199-207.
  • Rafińska, K., Pomastowski, P., Wrona, O., Górecki, R., & Buszewski, B. (2017). Medicago sativa as a source of secondary metabolites for agriculture and pharmaceutical industry Phytochemistry Letters 20, 520-539.
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine 26,1231-1237.
There are 31 citations in total.

Details

Primary Language English
Subjects Agricultural Engineering
Journal Section Articles
Authors

Hakime Hülya Orak 0000-0002-1803-1644

Magdalena Karamać 0000-0001-8313-3092

Adnan Orak 0000-0001-6588-8563

Hazım Serkan Tenikecier 0000-0002-1866-410X

Ryszard Amarowicz 0000-0001-9731-0045

Publication Date December 31, 2021
Acceptance Date December 7, 2021
Published in Issue Year 2021 Volume: 31 Issue: 4

Cite

APA Orak, H. H., Karamać, M., Orak, A., Tenikecier, H. S., et al. (2021). Antioxidant Activity and Total Phenolic Contents of Different Alfalfa (Medicago sativa L.) Varieties Grown in Turkey. Yuzuncu Yıl University Journal of Agricultural Sciences, 31(4), 995-1000. https://doi.org/10.29133/yyutbd.946487
Creative Commons License
Yuzuncu Yil University Journal of Agricultural Sciences by Van Yuzuncu Yil University Faculty of Agriculture is licensed under a Creative Commons Attribution 4.0 International License.