Research Article
BibTex RIS Cite
Year 2021, Volume: 2 Issue: 2, 1 - 13, 26.04.2021

Abstract

References

  • Amin, W., Ahmed, E., Embarak, M., Abo-Shama, U., Thabit, A., & Ismail, S. (2017). Molecular Detection of Enterotoxigenic E. coli in raw Milk and Milk Products. Int. J. Curr. Microbiol. App. Sci, 6(11), 856-864.
  • Bedada, B., & Hiko, A. (2011). Mastitis and antimicrobial susceptibility test at Asella, Oromia Regional state, Ethiopia. J. Microbiol. Antimicrob, 3(9), 228-232.
  • Cheng, X., He, W., Huang, F., Huang, M., & Zhou, G. (2014). Multiplex real-time PCR for the identification and quantification of DNA from duck, pig and chicken in Chinese blood curds. Food Research International, 60, 30-37.
  • Christensen, D. G., Orr, J. S., Rao, C. V., & Wolfe, A. J. J. A. E. M. (2017). Increasing growth yield and decreasing acetylation in Escherichia coli by optimizing the carbon-to-magnesium ratio in peptide-based media. 83(6), e03034-03016.
  • Dalmasso, A., Civera, T., La Neve, F., & Bottero, M. T. (2011). Simultaneous detection of cow and buffalo milk in mozzarella cheese by real-time PCR assay. Food Chemistry, 124(1), 362-366.
  • Dalmasso, A., Fontanella, E., Piatti, P., Civera, T., Rosati, S., & Bottero, M. T. (2004). A multiplex PCR assay for the identification of animal species in feedstuffs. Molecular and cellular probes, 18(2), 81-87.
  • Dell'Orco, F., Gusmara, C., Loiacono, M., Gugliotta, T., Albonico, F., Mortarino, M., & Zecconi, A. J. R. i. v. s. (2019). Evaluation of virulence factors profiles and antimicrobials resistance of Escherichia coli isolated from bulk tank milk and raw milk filters. 123, 77-83.
  • Di Pinto, A., Forte, V., Conversano, M., & Tantillo, G. (2005). Duplex polymerase chain reaction for detection of pork meat in horse meat fresh sausages from Italian retail sources. Food control, 16(5), 391-394.
  • Eckburg, P. B., Bik, E. M., Bernstein, C. N., Purdom, E., Dethlefsen, L., Sargent, M., . . . Relman, D. A. (2005). Diversity of the human intestinal microbial flora. Science, 308(5728), 1635-1638.
  • Gan, S. D., & Patel, K. R. (2013). Enzyme immunoassay and enzyme-linked immunosorbent assay. J Invest Dermatol, 133(9), e12.
  • Guetouache, M., Bettache, G., & Samir, M. (2014). Composition and nutritional value of raw milk. Issues Biol. Sci. Pharm. Res, 2(10), 115-122.
  • Heo, J., & Hua, S. Z. J. S. (2009). An overview of recent strategies in pathogen sensing. 9(6), 4483-4502.
  • Hossain, S. Z., Ozimok, C., Sicard, C., Aguirre, S. D., Ali, M. M., Li, Y., & Brennan, J. D. (2012). Multiplexed paper test strip for quantitative bacterial detection. Analytical and bioanalytical chemistry, 403(6), 1567-1576.
  • Islam, M., Kabir, S., & Seel, S. (2016). MOLECULAR DETECTION AND CHARACTERIZATION OF ESCHERICHIA COLI ISOLATED FROM RAW MILK SOLD IN DIFFERENT MARKETS OF BANGLADESH. Bangladesh Journal of Veterinary Medicine, 14(2), 271-275.
  • Kretzer, J. W., Lehmann, R., Schmelcher, M., Banz, M., Kim, K.-P., Korn, C., & Loessner, M. J. (2007). Use of high-affinity cell wall-binding domains of bacteriophage endolysins for immobilization and separation of bacterial cells. Applied and environmental microbiology, 73(6), 1992-2000.
  • Kumar, R., & Prasad, A. (2010). Detection of E. coli and Staphylococcus in milk and milk products in and around Pantnagar. Veterinary World, 3(11), 495.
  • Law, J. W.-F., Ab Mutalib, N.-S., Chan, K.-G., & Lee, L.-H. J. F. i. m. (2015). Rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations. 5, 770.
  • Lazcka, O., Del Campo, F. J., & Munoz, F. X. (2007). Pathogen detection: a perspective of traditional methods and biosensors. Biosensors and Bioelectronics, 22(7), 1205-1217.
  • Link, A. J., Jeong, K. J., & Georgiou, G. (2007). Beyond toothpicks: new methods for isolating mutant bacteria. Nature Reviews Microbiology, 5(9), 680.
  • Matsunaga, T., Chikuni, K., Tanabe, R., Muroya, S., Shibata, K., Yamada, J., & Shinmura, Y. (1999). A quick and simple method for the identification of meat species and meat products by PCR assay. Meat Science, 51(2), 143-148.
  • Memon, J., Yang, Y., Kashif, J., Yaqoob, M., Buriro, R., Soomro, J., . . . Hongjie, F. (2013). Genotypes, Virulence Factors and Antimicrobial Resistance Genes of Staphylococcus aureus Isolated in Bovine Subclinical Mastitis from Eastern China. Pakistan Veterinary Journal, 33(4).
  • Mortazavi, S. A., & Gharehyakheh, S. (2014). Comparison of microbial contamination of milk from traditional and industrial farms. European Journal of Experimental Biology, 4(1), 195-197.
  • Mungai, E. A., Behravesh, C. B., & Gould, L. H. J. E. i. d. (2015). Increased outbreaks associated with nonpasteurized milk, United States, 2007–2012. 21(1), 119.
  • Oliver, S. P., Jayarao, B. M., & Almeida, R. A. (2005). Foodborne pathogens in milk and the dairy farm environment: food safety and public health implications. Foodbourne Pathogens & Disease, 2(2), 115-129.
  • Ostergaard, L., Birkelund, S., & Christiansen, G. (1993). Use of polymerase chain reaction for detection of Chlamydia trachomatis. Journal of Clinical Microbiology, 31(11), 3081.
  • Paul, M., Van Hekken, D. L., & Brewster, J. D. (2013). Detection and quantitation of Escherichia coli O157 in raw milk by direct qPCR. International Dairy Journal, 32(2), 53-60.
  • Pingoud, A., & Jeltsch, A. (2001). Structure and function of type II restriction endonucleases. Nucleic acids research, 29(18), 3705-3727.
  • Pukančíková, L., Lipničanová, S., Kačániová, M., Chmelová, D., & Ondrejovič, M. (2016). Natural Microflora of Raw Cow Milk and their Enzymatic Spoilage Potential. Nova Biotechnologica et Chimica, 15(2), 142-155.
  • Razzaq, A., Shamsi, S., Nawaz, A., Ali, A., Malik, K. J. P., & Biology, A. (2016). Occurrence of Shiga toxin producing E. coli from raw milk. 5(2), 270.
  • Soares, S., Amaral, J. S., Mafra, I., & Oliveira, M. B. P. (2010). Quantitative detection of poultry meat adulteration with pork by a duplex PCR assay. Meat Science, 85(3), 531-536.
  • Soares, S., Amaral, J. S., Oliveira, M. B. P., & Mafra, I. (2013). A SYBR Green real-time PCR assay to detect and quantify pork meat in processed poultry meat products. Meat Science, 94(1), 115-120.
  • Soomro, A., Arain, M., Khaskheli, M., & Bhutto, B. (2002). Isolation of Escherichia coli from raw milk and milk products in relation to public health sold under market conditions at Tandojam. Pakistan Journal of Nutrition, 1(3), 151-152.
  • Srinivasan, R., Karaoz, U., Volegova, M., MacKichan, J., Kato-Maeda, M., Miller, S., . . . Lynch, S. V. J. P. o. (2015). Use of 16S rRNA gene for identification of a broad range of clinically relevant bacterial pathogens. 10(2), e0117617.
  • Tadesse, H. A., Gidey, N. B., Workelule, K., Hailu, H., Gidey, S., Bsrat, A., & Taddele, H. J. V. m. i. (2018). Antimicrobial Resistance Profile of E. coli Isolated from Raw Cow Milk and Fresh Fruit Juice in Mekelle, Tigray, Ethiopia. 2018.
  • Tahira, B., Ullah, K., Samad, A., Ali, S., Nabi, S., Naeem, M., . . . RESEARCH. (2017). ISOLATION AND MOLECULAR CHARACTERIZATION OF SHIGA TOXIN PRODUCING E. COLI O157: H7 IN RAW MILK USING mPCR. 8(7), 3107-3112.
  • Tamerat, N., Muktar, Y., & Shiferaw, D. (2016). Application of molecular diagnostic techniques for the detection of E. coli O157: H7: a review. J Vet Sci Technol, 7(362), 1-9.
  • Tenaillon, O., Skurnik, D., Picard, B., & Denamur, E. (2010). The population genetics of commensal Escherichia coli. Nature Reviews Microbiology, 8(3), 207.
  • Tunegová, M., Toman, R., & Tančin, V. (2016). HEAVY METALS–ENVIRONMENTAL CONTAMINANTS AND THEIR OCCURRENCE IN DIFFERENT TYPES OF MILK. Slovak Journal of, 122.
  • Wang, C., Xing, K., Zhang, G., Yuan, M., Xu, S., Liu, D., . . . Lai, W.-H. J. F. c. (2019). Novel ELISA based on fluorescent quenching of DNA-stabilized silver nanoclusters for detecting E. coli O157: H7. 281, 91-96.
  • Weintraub, A. (2007). Enteroaggregative Escherichia coli: epidemiology, virulence and detection. Journal of medical microbiology, 56(1), 4-8.
  • Yaici, L., Haenni, M., Saras, E., Boudehouche, W., Touati, A., & Madec, J.-Y. J. J. o. A. C. (2016). bla NDM-5-carrying IncX3 plasmid in Escherichia coli ST1284 isolated from raw milk collected in a dairy farm in Algeria. 71(9), 2671-2672.
  • Zafalon, L. F., LONGONI, H., Benvenutto, F., Castelani, L., & Broccolo, C. R. (2008). Aspectos epidemiológicos da mastite bovina causada por Staphylococcus aureus. Embrapa Pecuária Sudeste-Artigo em periódico indexado (ALICE).

Molecular characterization of Escherichia coli isolated from raw cow milk samples collected from district Bahawalpur, Pakistan

Year 2021, Volume: 2 Issue: 2, 1 - 13, 26.04.2021

Abstract

The present study was structured for molecular detection and characterization of Escherichia. coli (E. coli) isolated from cow’s raw milk samples. A total of 100 milk samples were collected in a way that 10 samples each were collected from markets of Jamalpur, Hasilpur, Khairpur, Qaimpur, Lal Sohanra, Lal Sohanra Park, Yazman Mandi, Rajkan, Ahmedpur East and Uch Sharif, district Bahawalpur, Pakistan. Milk samples were then cultured on different culture media for bacterial isolation. Identification of bacteria was done through Gram’s staining, properties of bacterial culture on different selective media and biochemical tests including catalase and coagulase tests. Final identification was performed through PCR and resolution of PCR products by gel electrophoresis. Out of 100 samples, 43 samples were found to be contaminated with E. coli. E. coli isolates were then amplified by 16S rRNA gene-based PCR. Antimicrobial sensitivity test was also performed to confirm the susceptibility of E. coli to different antibiotics. Results of the antimicrobial sensitivity test showed that the E. coli isolates were resistant to amoxicillin and erythromycin but sensitive to azithromycin, ciprofloxacin, gentamicin, norfloxacin and streptomycin.

References

  • Amin, W., Ahmed, E., Embarak, M., Abo-Shama, U., Thabit, A., & Ismail, S. (2017). Molecular Detection of Enterotoxigenic E. coli in raw Milk and Milk Products. Int. J. Curr. Microbiol. App. Sci, 6(11), 856-864.
  • Bedada, B., & Hiko, A. (2011). Mastitis and antimicrobial susceptibility test at Asella, Oromia Regional state, Ethiopia. J. Microbiol. Antimicrob, 3(9), 228-232.
  • Cheng, X., He, W., Huang, F., Huang, M., & Zhou, G. (2014). Multiplex real-time PCR for the identification and quantification of DNA from duck, pig and chicken in Chinese blood curds. Food Research International, 60, 30-37.
  • Christensen, D. G., Orr, J. S., Rao, C. V., & Wolfe, A. J. J. A. E. M. (2017). Increasing growth yield and decreasing acetylation in Escherichia coli by optimizing the carbon-to-magnesium ratio in peptide-based media. 83(6), e03034-03016.
  • Dalmasso, A., Civera, T., La Neve, F., & Bottero, M. T. (2011). Simultaneous detection of cow and buffalo milk in mozzarella cheese by real-time PCR assay. Food Chemistry, 124(1), 362-366.
  • Dalmasso, A., Fontanella, E., Piatti, P., Civera, T., Rosati, S., & Bottero, M. T. (2004). A multiplex PCR assay for the identification of animal species in feedstuffs. Molecular and cellular probes, 18(2), 81-87.
  • Dell'Orco, F., Gusmara, C., Loiacono, M., Gugliotta, T., Albonico, F., Mortarino, M., & Zecconi, A. J. R. i. v. s. (2019). Evaluation of virulence factors profiles and antimicrobials resistance of Escherichia coli isolated from bulk tank milk and raw milk filters. 123, 77-83.
  • Di Pinto, A., Forte, V., Conversano, M., & Tantillo, G. (2005). Duplex polymerase chain reaction for detection of pork meat in horse meat fresh sausages from Italian retail sources. Food control, 16(5), 391-394.
  • Eckburg, P. B., Bik, E. M., Bernstein, C. N., Purdom, E., Dethlefsen, L., Sargent, M., . . . Relman, D. A. (2005). Diversity of the human intestinal microbial flora. Science, 308(5728), 1635-1638.
  • Gan, S. D., & Patel, K. R. (2013). Enzyme immunoassay and enzyme-linked immunosorbent assay. J Invest Dermatol, 133(9), e12.
  • Guetouache, M., Bettache, G., & Samir, M. (2014). Composition and nutritional value of raw milk. Issues Biol. Sci. Pharm. Res, 2(10), 115-122.
  • Heo, J., & Hua, S. Z. J. S. (2009). An overview of recent strategies in pathogen sensing. 9(6), 4483-4502.
  • Hossain, S. Z., Ozimok, C., Sicard, C., Aguirre, S. D., Ali, M. M., Li, Y., & Brennan, J. D. (2012). Multiplexed paper test strip for quantitative bacterial detection. Analytical and bioanalytical chemistry, 403(6), 1567-1576.
  • Islam, M., Kabir, S., & Seel, S. (2016). MOLECULAR DETECTION AND CHARACTERIZATION OF ESCHERICHIA COLI ISOLATED FROM RAW MILK SOLD IN DIFFERENT MARKETS OF BANGLADESH. Bangladesh Journal of Veterinary Medicine, 14(2), 271-275.
  • Kretzer, J. W., Lehmann, R., Schmelcher, M., Banz, M., Kim, K.-P., Korn, C., & Loessner, M. J. (2007). Use of high-affinity cell wall-binding domains of bacteriophage endolysins for immobilization and separation of bacterial cells. Applied and environmental microbiology, 73(6), 1992-2000.
  • Kumar, R., & Prasad, A. (2010). Detection of E. coli and Staphylococcus in milk and milk products in and around Pantnagar. Veterinary World, 3(11), 495.
  • Law, J. W.-F., Ab Mutalib, N.-S., Chan, K.-G., & Lee, L.-H. J. F. i. m. (2015). Rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations. 5, 770.
  • Lazcka, O., Del Campo, F. J., & Munoz, F. X. (2007). Pathogen detection: a perspective of traditional methods and biosensors. Biosensors and Bioelectronics, 22(7), 1205-1217.
  • Link, A. J., Jeong, K. J., & Georgiou, G. (2007). Beyond toothpicks: new methods for isolating mutant bacteria. Nature Reviews Microbiology, 5(9), 680.
  • Matsunaga, T., Chikuni, K., Tanabe, R., Muroya, S., Shibata, K., Yamada, J., & Shinmura, Y. (1999). A quick and simple method for the identification of meat species and meat products by PCR assay. Meat Science, 51(2), 143-148.
  • Memon, J., Yang, Y., Kashif, J., Yaqoob, M., Buriro, R., Soomro, J., . . . Hongjie, F. (2013). Genotypes, Virulence Factors and Antimicrobial Resistance Genes of Staphylococcus aureus Isolated in Bovine Subclinical Mastitis from Eastern China. Pakistan Veterinary Journal, 33(4).
  • Mortazavi, S. A., & Gharehyakheh, S. (2014). Comparison of microbial contamination of milk from traditional and industrial farms. European Journal of Experimental Biology, 4(1), 195-197.
  • Mungai, E. A., Behravesh, C. B., & Gould, L. H. J. E. i. d. (2015). Increased outbreaks associated with nonpasteurized milk, United States, 2007–2012. 21(1), 119.
  • Oliver, S. P., Jayarao, B. M., & Almeida, R. A. (2005). Foodborne pathogens in milk and the dairy farm environment: food safety and public health implications. Foodbourne Pathogens & Disease, 2(2), 115-129.
  • Ostergaard, L., Birkelund, S., & Christiansen, G. (1993). Use of polymerase chain reaction for detection of Chlamydia trachomatis. Journal of Clinical Microbiology, 31(11), 3081.
  • Paul, M., Van Hekken, D. L., & Brewster, J. D. (2013). Detection and quantitation of Escherichia coli O157 in raw milk by direct qPCR. International Dairy Journal, 32(2), 53-60.
  • Pingoud, A., & Jeltsch, A. (2001). Structure and function of type II restriction endonucleases. Nucleic acids research, 29(18), 3705-3727.
  • Pukančíková, L., Lipničanová, S., Kačániová, M., Chmelová, D., & Ondrejovič, M. (2016). Natural Microflora of Raw Cow Milk and their Enzymatic Spoilage Potential. Nova Biotechnologica et Chimica, 15(2), 142-155.
  • Razzaq, A., Shamsi, S., Nawaz, A., Ali, A., Malik, K. J. P., & Biology, A. (2016). Occurrence of Shiga toxin producing E. coli from raw milk. 5(2), 270.
  • Soares, S., Amaral, J. S., Mafra, I., & Oliveira, M. B. P. (2010). Quantitative detection of poultry meat adulteration with pork by a duplex PCR assay. Meat Science, 85(3), 531-536.
  • Soares, S., Amaral, J. S., Oliveira, M. B. P., & Mafra, I. (2013). A SYBR Green real-time PCR assay to detect and quantify pork meat in processed poultry meat products. Meat Science, 94(1), 115-120.
  • Soomro, A., Arain, M., Khaskheli, M., & Bhutto, B. (2002). Isolation of Escherichia coli from raw milk and milk products in relation to public health sold under market conditions at Tandojam. Pakistan Journal of Nutrition, 1(3), 151-152.
  • Srinivasan, R., Karaoz, U., Volegova, M., MacKichan, J., Kato-Maeda, M., Miller, S., . . . Lynch, S. V. J. P. o. (2015). Use of 16S rRNA gene for identification of a broad range of clinically relevant bacterial pathogens. 10(2), e0117617.
  • Tadesse, H. A., Gidey, N. B., Workelule, K., Hailu, H., Gidey, S., Bsrat, A., & Taddele, H. J. V. m. i. (2018). Antimicrobial Resistance Profile of E. coli Isolated from Raw Cow Milk and Fresh Fruit Juice in Mekelle, Tigray, Ethiopia. 2018.
  • Tahira, B., Ullah, K., Samad, A., Ali, S., Nabi, S., Naeem, M., . . . RESEARCH. (2017). ISOLATION AND MOLECULAR CHARACTERIZATION OF SHIGA TOXIN PRODUCING E. COLI O157: H7 IN RAW MILK USING mPCR. 8(7), 3107-3112.
  • Tamerat, N., Muktar, Y., & Shiferaw, D. (2016). Application of molecular diagnostic techniques for the detection of E. coli O157: H7: a review. J Vet Sci Technol, 7(362), 1-9.
  • Tenaillon, O., Skurnik, D., Picard, B., & Denamur, E. (2010). The population genetics of commensal Escherichia coli. Nature Reviews Microbiology, 8(3), 207.
  • Tunegová, M., Toman, R., & Tančin, V. (2016). HEAVY METALS–ENVIRONMENTAL CONTAMINANTS AND THEIR OCCURRENCE IN DIFFERENT TYPES OF MILK. Slovak Journal of, 122.
  • Wang, C., Xing, K., Zhang, G., Yuan, M., Xu, S., Liu, D., . . . Lai, W.-H. J. F. c. (2019). Novel ELISA based on fluorescent quenching of DNA-stabilized silver nanoclusters for detecting E. coli O157: H7. 281, 91-96.
  • Weintraub, A. (2007). Enteroaggregative Escherichia coli: epidemiology, virulence and detection. Journal of medical microbiology, 56(1), 4-8.
  • Yaici, L., Haenni, M., Saras, E., Boudehouche, W., Touati, A., & Madec, J.-Y. J. J. o. A. C. (2016). bla NDM-5-carrying IncX3 plasmid in Escherichia coli ST1284 isolated from raw milk collected in a dairy farm in Algeria. 71(9), 2671-2672.
  • Zafalon, L. F., LONGONI, H., Benvenutto, F., Castelani, L., & Broccolo, C. R. (2008). Aspectos epidemiológicos da mastite bovina causada por Staphylococcus aureus. Embrapa Pecuária Sudeste-Artigo em periódico indexado (ALICE).
There are 42 citations in total.

Details

Primary Language English
Subjects Food Engineering
Journal Section Research Articles
Authors

Bushra Parveen This is me

Yasmeen Junejo This is me

Muhammad Safdar

Mehmet Özaslan

Publication Date April 26, 2021
Submission Date April 11, 2021
Published in Issue Year 2021 Volume: 2 Issue: 2

Cite

EndNote Parveen B, Junejo Y, Safdar M, Özaslan M (April 1, 2021) Molecular characterization of Escherichia coli isolated from raw cow milk samples collected from district Bahawalpur, Pakistan. Zeugma Biological Science 2 2 1–13.