Cilt 18, Sayı 1, Sayfalar 31 - 34 2015-11-02

Using Culture and PCR Technique for Detection of Escherichia coli O157:H7 in Duhok Cheese

Özlem TURGAY [1] , Sowayla MIKAEEL [2]

150 117

ABSTRACT: This study was aimed to investigate the presence of Escherichia coli O157:H7 in Duhok cheese by culture and Polymerase Chain Reaction (PCR) technique. The cheese samples (n:120) were purchased from different supermarkets and shops from Zakho, Akre, Amedy and Duhok cities during September 2011 to February 2012.

X-gal positive, MUG negative, indole positive colonies were found as 10% (12/120) of samples. These colonies were confirmed by biochemical tests (API E 20 test, latex agglutination tests) and results showed that 7 (5.83%) of E. coli isolates from cheese were positive for O157:H7. These isolates were also confirmed by PCR assay using specific primers for rfbO157 and flicH7. The numbers of positive isolates for rfbO157 and flicH7 were 3.33 and 0.83%, respectively. The PCR results confirmed as 0.83% of samples were E. coli O157:H7.

Key words: Duhok cheese, Escherichia coli O157:H7, Cultural technique, PCR technique

 

Kültür ve PZR Yöntemi ile Duhok Peynirinde Escherichia coli O157:H7'nin Araştırılması

 

ÖZET: Bu çalışmada Duhok peynirinde of E. coli O157:H7'nin varlığı kültür ve Polimeraz Zincir Reaksiyonu tekniği (PZR) ile araştırılmıştır. Peynir örnekleri (n:120) Zakho, Akre, Amedy ve Duhok şehirlerinden Eylül 2011-Şubat 2012 tarihleri arasında yerel market ve bakkallardan temin edilmiştir.

X-gal pozitif, MUG negatif,  indol pozitif olan %10 (12/120) oranında şüpheli koloni tespit edilmiştir. Şüpheli kolonilere biyokimyasal testler uygulanmış (API E 20 ve latex aglütinasyon testi) ve 7 (%5,83) örnekte E. coli O157:H7 tespit edilmiştir. Bu izolatlararfbO157 ve flicH7 primerleri kullanılarak PCR yöntemi ile doğrulama işlemi uygulanmıştır. İzolatlar %3,33 oranında O157 ve 0,83 oranında H7 antijeni pozitif olarak tespit edilmiştir. PCR analizlerinin sonunda örneklerin %0,83'ünün E. coli O157:H7 olduğu gözlenmiştir.

Anahtar Kelimeler: Duhok Peyniri, Escherichia coli O157:H7, Kültür tekniği, PZR tekniği

Bu çalışmada Duhok peynirinde of E. coli O157:H7'nin varlığı kültür ve Polimeraz Zincir Reaksiyonutekniği (PZR) ile araştırılmıştır. Peynir örnekleri (n:120) Zakho, Akre, Amedy ve Duhok şehirlerinden Eylül 2011Şubat 2012 tarihleri arasında yerel market ve bakkallardan temin edilmiştir.X-gal pozitif, MUG negatif, indol pozitif olan %10 (12/120) oranında şüpheli koloni tespit edilmiştir. Şüphelikolonilere biyokimyasal testler uygulanmış (API E 20 ve latex aglütinasyon testi) ve 7 (%5,83) örnekte E. coliO157:H7 tespit edilmiştir. Bu izolatlararfbO157 ve flicH7 primerleri kullanılarak PCR yöntemi ile doğrulama işlemiuygulanmıştır. İzolatlar %3,33 oranında O157 ve 0,83 oranında H7 antijeni pozitif olarak tespit edilmiştir. PCRanalizlerinin sonunda örneklerin %0,83'ünün E. coli O157:H7 olduğu gözlenmiştir
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Birincil Dil en
Konular
Dergi Bölümü MİKROBİYOLOJİ (Microbiology)
Yazarlar

Yazar: Özlem TURGAY
E-posta: ozlem@ksu.edu.tr

Yazar: Sowayla MIKAEEL
E-posta: info@duhok.vet.org

Bibtex @ { ksudobil243867, journal = {Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi}, issn = {}, address = {Kahramanmaraş Sütçü İmam Üniversitesi}, year = {2015}, volume = {18}, pages = {31 - 34}, doi = {10.18016/ksujns.94848}, title = {Using Culture and PCR Technique for Detection of Escherichia coli O157:H7 in Duhok Cheese}, language = {en}, key = {cite}, author = {TURGAY, Özlem and MIKAEEL, Sowayla} }
APA TURGAY, Ö , MIKAEEL, S . (2015). Using Culture and PCR Technique for Detection of Escherichia coli O157:H7 in Duhok Cheese. Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi, 18 (1), 31-34. Retrieved from http://dogadergi.ksu.edu.tr/issue/22844/243867
MLA TURGAY, Ö , MIKAEEL, S . "Using Culture and PCR Technique for Detection of Escherichia coli O157:H7 in Duhok Cheese". Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi 18 (2015): 31-34 <http://dogadergi.ksu.edu.tr/issue/22844/243867>
Chicago TURGAY, Ö , MIKAEEL, S . "Using Culture and PCR Technique for Detection of Escherichia coli O157:H7 in Duhok Cheese". Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi 18 (2015): 31-34
RIS TY - JOUR T1 - Using Culture and PCR Technique for Detection of Escherichia coli O157:H7 in Duhok Cheese AU - Özlem TURGAY , Sowayla MIKAEEL Y1 - 2015 PY - 2015 N1 - DO - T2 - Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi JF - Journal JO - JOR SP - 31 EP - 34 VL - 18 IS - 1 SN - -1309-1743 M3 - UR - Y2 - 2017 ER -
EndNote %0 Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi Using Culture and PCR Technique for Detection of Escherichia coli O157:H7 in Duhok Cheese %A Özlem TURGAY , Sowayla MIKAEEL %T Using Culture and PCR Technique for Detection of Escherichia coli O157:H7 in Duhok Cheese %D 2015 %J Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi %P -1309-1743 %V 18 %N 1 %R %U