Cilt 20, Sayı 4, Sayfalar 361 - 367 2017-04-10

Evaluation of Antioxidant and Cytotoxic Properties of Primula vulgaris Leaf Extract
Primula vulgaris Yaprak Ekstraktının Antioksidan ve Sitotoksik Özelliklerinin Değerlendirilmesi

Selim DEMİR [1] , İbrahim TURAN [2] , Rezzan ALİYAZICIOĞLU [3] , Yüksel ALİYAZICIOĞLU [4]

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Primula vulgaris belongs to the genus Primula and this genus is frequently used in traditional medicine. Numerous studies reported the antioxidant and cytotoxic effects of various extracts of different Primula species but, there were limited studies of the antioxidant and cytotoxic effect of P. vulgaris. The aim of this study was to determine the antioxidant properties and cytotoxic effects of dimethyl sulfoxide extract of leaves of P. vulgaris. The total polyphenolic content (TPC), total flavonoid content (TFC) and reducing antioxidant power of extract were evaluated using spectrophotometric procedures. The cytotoxic effects of extract were revealed on human lung (A549), liver (HepG2), breast (MCF-7), prostate (PC-3), and colon (WiDr) cancer cell lines and normal fibroblast cells using thiazolyl blue tetrazolium bromide (MTT) assay. TPC and FRAP values were 1890.1±11.2 mg gallic acid equivalents and 3233.1±176.2 mg trolox equivalents per 100 g sample, respectively. The extract showed selective cytotoxic effects on all studied cancer cell lines compared to normal fibroblast cells and IC50 values ranged from 133.3-253.8 µg/mL. This is the first study to reveal the antioxidant properties of P. vulgaris leaf extract and its cytotoxic effect on cancer cell lines. Further studies are now needed to identify the antioxidant and cytotoxic molecules in the extract and their mechanisms.

Primula vulgaris, Primula cinsine mensup bir bitki olup, bu bitkiler sıklıkla geleneksel tedavide kullanılmaktadırlar. Farklı Primula türlerinin farklı ekstraktlarının antioksidan ve sitotoksik etkilerini konu alan çok sayıda çalışma olmasına rağmen, P. vulgaris ekstraktlarının bu özellikleri ile ilgili sınırlı sayıda çalışma bulunmaktadır. Bu çalışmanın amacı P. vulgaris yapraklarının dimetil sülfoksitli ekstraktının antioksidan özelliklerinin ve sitotoksik etkilerinin belirlenmesidir. Ekstraktın toplam fenolik madde miktarı, toplam flavonoid madde miktarı ve indirgeyici antioksidan güç tayini spektrofotometrik yöntemler kullanılarak belirlendi. Ekstraktın sitotoksik etkisi ise insan akciğer (A549), karaciğer (HepG2), meme (MCF-7), prostat (PC-3) ve kolon (WiDr) kanser hücre serileri ile normal insan fibroblast hücre serisi üzerinde MTT yöntemi kullanılarak değerlendirildi. Ekstraktın toplam fenolik madde içeriği ve indirgeyici antioksidan güç değerleri 100 g örnek başına sırasıyla 1890.1±11.2 mg gallik asit eşdeğeri ve 3233.1±176.2 mg troloks eşdeğeri olarak bulundu. Ekstrakt çalışılan tüm kanser hücre serileri üzerinde normal fibroblast hücrelerine göre seçici sitotoksik etki gösterdi ve IC50 değerleri 133.3-253.8 µg/mL arasında hesaplandı. Bu çalışma P. vulgaris yaprak ekstraktının antioksidan özelliklerini ve kanser hücre serileri üzerindeki sitotoksik etkisini ortaya koyan ilk çalışmadır. Ekstraktta bulunan antioksidan ve sitotoksik etki gösteren molekülleri ve bu etkilerin mekanizmalarını belirleyebilmek için daha ileri çalışmalar gerekmektedir.

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Konular Yaşam Bilimleri
Dergi Bölümü MİKROBİYOLOJİ (Microbiology)
Yazarlar

Yazar: Selim DEMİR
E-posta: selim-demir@hotmail.com
Kurum: KARADENIZ TEKNIK UNIV
Ülke: Turkey


Yazar: İbrahim TURAN
E-posta: ibrahimtrn@gmail.com
Kurum: GUMUSHANE UNIV

Yazar: Rezzan ALİYAZICIOĞLU
E-posta: rezzanaoglu@mynet.com
Kurum: KARADENIZ TEKNIK UNIV

Yazar: Yüksel ALİYAZICIOĞLU
E-posta: yukselayazici@hotmail.com
Kurum: KARADENIZ TEKNIK UNIV

Bibtex @araştırma makalesi { ksudobil288175, journal = {Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi}, issn = {}, address = {Kahramanmaraş Sütçü İmam Üniversitesi}, year = {2017}, volume = {20}, pages = {361 - 367}, doi = {10.18016/ksudobil.288175}, title = {Evaluation of Antioxidant and Cytotoxic Properties of Primula vulgaris Leaf Extract}, language = {en}, key = {cite}, author = {TURAN, İbrahim and ALİYAZICIOĞLU, Yüksel and DEMİR, Selim and ALİYAZICIOĞLU, Rezzan} } @araştırma makalesi { ksudobil288175, journal = {Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi}, issn = {}, address = {Kahramanmaraş Sütçü İmam Üniversitesi}, year = {2017}, volume = {20}, pages = {361 - 367}, doi = {10.18016/ksudobil.288175}, title = {Primula vulgaris Yaprak Ekstraktının Antioksidan ve Sitotoksik Özelliklerinin Değerlendirilmesi}, language = {tr}, key = {cite}, author = {TURAN, İbrahim and ALİYAZICIOĞLU, Yüksel and DEMİR, Selim and ALİYAZICIOĞLU, Rezzan} }
APA DEMİR, S , TURAN, İ , ALİYAZICIOĞLU, R , ALİYAZICIOĞLU, Y . (2017). Evaluation of Antioxidant and Cytotoxic Properties of Primula vulgaris Leaf Extract. Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi, 20 (4), 361-367. DOI: 10.18016/ksudobil.288175
MLA DEMİR, S , TURAN, İ , ALİYAZICIOĞLU, R , ALİYAZICIOĞLU, Y . "Evaluation of Antioxidant and Cytotoxic Properties of Primula vulgaris Leaf Extract". Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi 20 (2017): 361-367 <http://dogadergi.ksu.edu.tr/issue/29797/288175>
Chicago DEMİR, S , TURAN, İ , ALİYAZICIOĞLU, R , ALİYAZICIOĞLU, Y . "Evaluation of Antioxidant and Cytotoxic Properties of Primula vulgaris Leaf Extract". Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi 20 (2017): 361-367
RIS TY - JOUR T1 - Primula vulgaris Yaprak Ekstraktının Antioksidan ve Sitotoksik Özelliklerinin Değerlendirilmesi AU - Selim DEMİR , İbrahim TURAN , Rezzan ALİYAZICIOĞLU , Yüksel ALİYAZICIOĞLU Y1 - 2017 PY - 2017 N1 - doi: 10.18016/ksudobil.288175 DO - 10.18016/ksudobil.288175 T2 - Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi JF - Journal JO - JOR SP - 361 EP - 367 VL - 20 IS - 4 SN - -1309-1743 M3 - doi: 10.18016/ksudobil.288175 UR - http://dx.doi.org/10.18016/ksudobil.288175 Y2 - 2017 ER -
EndNote %0 Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi Primula vulgaris Yaprak Ekstraktının Antioksidan ve Sitotoksik Özelliklerinin Değerlendirilmesi %A Selim DEMİR , İbrahim TURAN , Rezzan ALİYAZICIOĞLU , Yüksel ALİYAZICIOĞLU %T Primula vulgaris Yaprak Ekstraktının Antioksidan ve Sitotoksik Özelliklerinin Değerlendirilmesi %D 2017 %J Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi %P -1309-1743 %V 20 %N 4 %R doi: 10.18016/ksudobil.288175 %U 10.18016/ksudobil.288175