Araştırma Makalesi
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Lavandula × intermedia’da Terpen Biyosentezi ile İlgili Genlerin Tanımlanması ve Moleküler Karakterizasyonu

Yıl 2023, Cilt: 54 Sayı: 3 - Research in Agricultural Sciences, 103 - 112, 19.10.2023

Damla Önder Sercan Önder Yaşar Karakurt

https://doi.org/10.5152/AUAF.2023.23150

Öz

Lavandin (Lavandula × intermedia), içerdiği koku bileşenleri nedeniyle kozmetik ve ilaç endüstrileri için ekonomik değeri yüksek olan bir uçucu yağ bitkisidir. Bu uçucu yağlar esas olarak geraniol, linalool ve linalil asetat dahil olmak üzere monoterpenler tarafından düzenlenir. Lavandinin uçucu yağ miktarı L. angustifolia’dan beş kat daha fazladır. Buna rağmen en kaliteli ve en çok talep edilen lavanta yağı nispeten yüksek linalool ve linalil asetat içeriği nedeniyle L. angustifolia’dan elde edilmektedir. Bu çalışmada, lavandin çiçeklerinin glandüler trikomlarından geranil difosfat sentaz (GPPS), linalool sentaz (LIS), linalil asetil transferaz (LAT) genlerini kodlayan genler izole edilmiştir. Sonuçlar LiGPP (L. × intermedia geranil difosfat sentaz), LiLIS (L. × intermedia linalool sentaz) ve LiLAT (L. × intermedia linalil asetil transferaz) genlerinin tomurcuklanmadan çiçek gelişiminin tam açma aşamasına kadar ifade edildiğini ve LiLIS geninin ifadesinin LiGPP ve LiLAT ifadelerine kıyasla daha düşük olduğunu göstermiştir. Filogenetik analizler, LiLIS geninin L. angustifolia’daki LIS geni ile benzer seviyelerde olduğunu, LiGPP ve LiLAT genlerinin ise diğer lavander türlerindeki GPP ve LAT genlerinden farklı seviyede bulunduğunu göstermiştir. Lavandinde terpen sentaz genlerinin tanımlanması, metabolik mühendislik yoluyla lavandindeki uçucu yağ bileşiminin kalitesinin
iyileştirilmesi için temel oluşturma potansiyeline sahiptir.

Anahtar Kelimeler

Uçucu yağ gen klonlama ve ifadesi geranil difosfat sentaz lavandin linalool sentaz linalil asetil transferaz

Kaynakça

  • Adal, A. M., Sarker, L. S., Lemke, A. D., & Mahmoud, S. S. (2017). Isolation and functional characterization of a methyl jasmonate-responsive 3-carene synthase from Lavandula x intermedia. Plant Molecular Biology, 93(6), 641–657. [CrossRef]
  • Baydar, H. (2009). Tıbbi ve Aromatik Bitkiler Bilimi ve Teknolojisi (Genişletilmiş 3. Baskı, Vol. 51, pp. 194–212). SDÜ Yayınları.
  • Baydar, H., & Kineci, S. (2009). Scent composition of essential oil, concrete, absolute and hydrosol from lavandin (Lavandula x intermedia Emeric ex Loisel.). Journal of Essential Oil Bearing Plants, 12(2), 131–136. [CrossRef]
  • Beetham, J., & Entwistle, T. (1982). The cultivated lavenders (1st ed, Entwistle). Royal Botanic Gardens.
  • Blažeković, B., Stabentheiner, E., Brantner, A., & Vladimir-Knezevic, S. (2012). A Comparative Study on Glandular Trichomes of Lavandula x intermedia, Budrovka’ and L. angustifolia. In Phyton-Annales Rei Botanicae 52(2), 227–244.
  • Boeckelmann, A. (2008). Monoterpene production and regulation in lavenders (Lavandula angustifolia and Lavandula x intermedia) (Doctoral Dissertation). University of British Columbia.https://doi.org/10.14288/1.0066802
  • Burke, C. C., Wildung, M. R., & Croteau, R. (1999). Geranyl diphosphate synthase: Cloning, expression, and characterization of this prenyltransferase as a heterodimer. Proceedings of the National Academy of Sciences of the United States of America, 96(23), 13062–13067. [CrossRef]
  • Crowell, A. L., Williams, D. C., Davis, E. M., Wildung, M. R., & Croteau, R. (2002). Molecular cloning and characterization of a new linalool synthase. Archives of Biochemistry and biophysics, 405(1), 112–121. [CrossRef]
  • D’Auria, J. C. (2006). Acyltransferases in plants: A good time to be BAHD. Current Opinion in Plant Biology, 9(3), 331–340. [CrossRef]
  • Demissie, Z. A. (2014). Cloning and functional characterization of genes involved in the biosynthesis and secretion of essential oil constituents of Lavandula (Doctoral Dissertation). University of British Columbia. https://doi.org./10.14288/1.0074341
  • Dudareva, N., Cseke, L., Blanc, V. M., & Pichersky, E. (1996). Evolution of floral scent in Clarkia: Novel patterns of S-linalool synthase gene expression in the C. breweri flower. Plant Cell, 8(7), 1137–1148. [CrossRef]
  • Gang, D. R., Wang, J., Dudareva, N., Nam, K. H., Simon, J. E., Lewinsohn, E., & Pichersky, E. (2001). An investigation of the storage and biosynthesis of phenylpropenes in sweet basil. Plant Physiology, 125(2), 539–555. [CrossRef]
  • Gershenzon, J., McCaskill, D., Rajaonarivony, J. I., Mihaliak, C., Karp, F., & Croteau, R. (1992). Isolation of secretory cells from plant glandular trichomes and their use in biosynthetic studies of monoterpenes and other gland products. Analytical Biochemistry, 200(1), 130–138. [CrossRef]
  • Guenther, E. (1952). The essential oils (Vol. 5). Robert E. (pp. 3-38) Krieger publishing company. Hall, T. (2004). BioEdit version 7.0. 0. Distributed by the Author. [CrossRef]
  • Accessed June 13, 2020.Hsiao, Y. Y., Jeng, M. F., Tsai, W. C., Chuang, Y. C., Li, C. Y., Wu, T. S., Kuoh, C. S., Chen, W. H., & Chen, H. H. (2008). A novel homodimeric geranyl diphosphate synthase from the orchid Phalaenopsis bellina lacking a DD (X) 2–4D motif. Plant Journal, 55(5), 719–733.
  • Jeanmougin, F., Thompson, J. D., Gouy, M., Higgins, D. G., & Gibson, T. J. (1998). Multiple sequence alignment with Clustal X. Trends in Biochemical Sciences, 23(10), 403–405. [CrossRef]
  • Jones, S., & Thornton, J. M. (1996). Principles of protein-protein interactions. Proceedings of the National Academy of Sciences of the United States of America, 93(1), 13–20. [CrossRef]
  • Kamran, H. M., Hussain, S. B., Junzhong, S., Xiang, L., & Chen, L. Q. (2020). Identification and molecular characterization of geranyl diphosphate synthase (GPPS) genes in wintersweet flower. Plants, 9(5), 666. [CrossRef] Kellogg, B. A., & Poulter, C. D. (1997). Chain elongation in the isoprenoid biosynthetic pathway. Current Opinion in Chemical Biology, 1(4), 570–578. [CrossRef]
  • Kıvrak, Ş. (2018). Essential oil composition and antioxidant activities of eight cultivars of Lavender and Lavandin from western Anatolia. Industrial Crops and Products, 117, 88–96. [CrossRef] Lafhal, S., Vanloot, P., Bombarda, I., Kister, J., & Dupuy, N. (2016). Identification of metabolomic markers of lavender and lavandin essential oils using mid-infrared spectroscopy. Vibrational Spectroscopy, 85, 79–90. [CrossRef]
  • Lane, A., Boecklemann, A., Woronuk, G. N., Sarker, L., & Mahmoud, S. S. (2010). A genomics resource for investigating regulation of essential oil production in Lavandula angustifolia. Planta, 231(4), 835–845. [CrossRef]
  • Lin, Y. L., Lee, Y. R., Huang, W. K., Chang, S. T., & Chu, F. H. (2014). Characterization of S-(+)-linalool synthase from several provenances of Cinnamomum osmophloeum. Tree Genetics and Genomes, 10(1), 75–86.[CrossRef]
  • Magnard, J. L., Bony, A. R., Bettini, F., Campanaro, A., Blerot, B., Baudino, S., & Jullien, F. (2018). Linalool and linalool nerolidol synthases in roses, several genes for little scent. Plant physiology and Biochemistry, 127, 74–87. [CrossRef]
  • Muñoz-Bertomeu, J., Arrillaga, I., Ros, R., & Segura, J. (2006). Upregulation of 1-deoxy-D-xylulose-5-phosphate synthase enhances production of essential oils in transgenic spike lavender. Plant Physiology, 142(3), 890–900. [CrossRef]
  • Renaud, E. N. C., Charles, D. J., & Simon, J. E. (2001). Essential oil quantity and composition from 10 cultivars of organically grown lavender and lavandin. Journal of Essential Oil research, 13(4), 269–273. [CrossRef]
  • Schmittgen, T. D., & Livak, K. J. (2008). Analyzing real-time PCR data by the comparative C(T) method. Nature Protocols, 3(6), 1101–1108. [CrossRef] Sarker, L. S., Galata, M., Demissie, Z. A., & Mahmoud, S. S. (2012). Molecular cloning and functional characterization of borneol dehydrogenase from the glandular trichomes of Lavandula x intermedia. Archives of Biochemistry and Biophysics, 528(2), 163–170. [CrossRef]
  • Sarker, L. S. (2013). Cloning of Lavandula essential oil biosynthetic genes(Doctoral Dissertation). University of British Columbia. https://dx.doi.org/10.14288/1.0073585
  • Schilmiller, A. L., Schauvinhold, I., Larson, M., Xu, R., Charbonneau, A. L., Schmidt, A., Wilkerson, C., Last, R. L., & Pichersky, E. (2009). Monoterpenes in the glandular trichomes of tomato are synthesized from a neryl diphosphate precursor rather than geranyl diphosphate. Proceedings of the National Academy of Sciences of the United States of America, 106(26), 10865–10870. [CrossRef]
  • Strommer, J., Gregerson, R., & Vayda, M. (1993). Isolation and characterization of plant mRNA. Methods in Plant Molecular Biology and Biotechnology, 49–65, Boca Raton, FL, USA: CRC
  • Sugiura, M., Ito, S., Saito, Y., Niwa, Y., Koltunow, A. M., Sugimoto, O., & Sakai, H. (2011). Molecular cloning and characterization of a linalool synthase from lemon myrtle. Bioscience, Biotechnology, and Biochemistry, 75(7), 1245–1248. [CrossRef]
  • Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30(12), 2725–2729. [CrossRef] Tholl, D., & Lee, S. (2011). Terpene specialized metabolism in Arabidopsis thaliana. Arabidopsis Book, 9, e0143. [CrossRef]
  • Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994). Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22(22), 4673–4680. [CrossRef]
  • Tucker, A. O. (1985). Lavender, spike, and lavandin. Herbarist, 51, 44–50.
  • Unno, H., Ichimaida, F., Suzuki, H., Takahashi, S., Tanaka, Y., Saito, A., Nishino, T., Kusunoki, M., & Nakayama, T. (2007). Structural and mutational studies of anthocyanin malonyl transferases establish the features of BAHD enzyme catalysis. Journal of Biological Chemistry, 282(21), 15812–15822. [CrossRef]
  • Upson, T., Andrews, S., Harriott, G., King, C., & Langhorne, J. (2004). The genus Lavandula (pp. 442). Timber Press.
  • van Schie, C. C., Haring, M. A., & Schuurink, R. C. (2007). Tomato linalool synthase is induced in trichomes by jasmonic acid. Plant Molecular Biology, 64(3), 251–263. [CrossRef]
  • Wang, G., & Dixon, R. A. (2009). Heterodimeric geranyl(geranyl)diphosphate synthase from hop (Humulus lupulus) and the evolution of monoterpene biosynthesis. Proceedings of the National Academy of Sciences of the United States of America, 106(24), 9914–9919. [CrossRef]
  • Weiss, E. A. (1997). Essential oil crops (pp. 600). CAB International. Whittington, D. A., Wise, M. L., Urbansky, M., Coates, R. M., Croteau, R. B., & Christianson, D. W. (2002). Bornyl iphosphate synthase: Structure and strategy for carbocation manipulation by a terpenoid cyclase. Proceedings of the National Academy of Sciences of the United States of America, 99(24), 15375–15380. [CrossRef]
  • Wu, T., Wang, Y., & Guo, D. (2012). Investigation of glandular trichome proteins in Artemisia annua L. using comparative proteomics. PLoS One, 7(8), e41822. [CrossRef]
  • Zaks, A., Davidovich-Rikanati, R., Bar, E., Inbar, M., & Lewinsohn, E. (2008). Biosynthesis of linalyl acetate and other terpenes in lemon mint (Mentha aquatica var. citrata, Lamiaceae) glandular trichomes. Israel Journal of Plant Sciences, 56(3), 233–244. [CrossRef]

Identification and Molecular Characterization of Genes Related to Terpene Biosynthesis From Lavandula × intermedia

Yıl 2023, Cilt: 54 Sayı: 3 - Research in Agricultural Sciences, 103 - 112, 19.10.2023

Damla Önder Sercan Önder Yaşar Karakurt

https://doi.org/10.5152/AUAF.2023.23150

Öz

Lavandin (Lavandula × intermedia Emeric ex Loisel.) is an essential oil-bearing plant with high economic value for the cosmetics and pharmaceutical industries due to its fragrance components. These essential oils primarily contain monoterpenes, including geraniol, linalool, and linalyl acetate. The essential oil content of Lavandula × intermedia is five times higher than that of Lavandula angustifolia oil. However, due to its relatively high content of linalool and linalyl acetate, the highest quality and sought-after lavender oil is produced from Lavandula angustifolia. In this study, the genes encoding geranyl diphosphate synthase, linalool synthase, and linalyl acetyl transferase were isolated from glandular trichomes of lavandin flowers. The results showed that Lavandula × intermediageranyl diphosphate synthase, Lavandula × intermedia linalool synthase, and Lavandula × intermedia linalyl acetyl transferase demonstrated expressions from the budding to the full blooming stage of flower development, and the expression levels of Lavandula × intermedia linalool synthase were lower in comparison with the expressions of Lavandula × intermedia geranyl diphosphate synthase and Lavandula × intermedia linalyl acetyl transferase. Phylogenetic analyses showed that Lavandula × intermedia linalool synthase and Lavandula angustifolia linalool synthase were at similar levels, while Lavandula × intermedia geranyl diphosphate synthase, Lavandula × intermedia linalyl acetyl transferase, and other lavender species geranyl diphosphate and linalyl acetyl transferase were different. The identification of these terpene synthases from lavandin forms the basis for improving the quality of essential oil composition in lavandin through metabolic engineering.

Anahtar Kelimeler

Essential oil gene cloning and expression geranyl diphosphate synthase lavandin linalool synthase linalyl acetyl transferase

Kaynakça

  • Adal, A. M., Sarker, L. S., Lemke, A. D., & Mahmoud, S. S. (2017). Isolation and functional characterization of a methyl jasmonate-responsive 3-carene synthase from Lavandula x intermedia. Plant Molecular Biology, 93(6), 641–657. [CrossRef]
  • Baydar, H. (2009). Tıbbi ve Aromatik Bitkiler Bilimi ve Teknolojisi (Genişletilmiş 3. Baskı, Vol. 51, pp. 194–212). SDÜ Yayınları.
  • Baydar, H., & Kineci, S. (2009). Scent composition of essential oil, concrete, absolute and hydrosol from lavandin (Lavandula x intermedia Emeric ex Loisel.). Journal of Essential Oil Bearing Plants, 12(2), 131–136. [CrossRef]
  • Beetham, J., & Entwistle, T. (1982). The cultivated lavenders (1st ed, Entwistle). Royal Botanic Gardens.
  • Blažeković, B., Stabentheiner, E., Brantner, A., & Vladimir-Knezevic, S. (2012). A Comparative Study on Glandular Trichomes of Lavandula x intermedia, Budrovka’ and L. angustifolia. In Phyton-Annales Rei Botanicae 52(2), 227–244.
  • Boeckelmann, A. (2008). Monoterpene production and regulation in lavenders (Lavandula angustifolia and Lavandula x intermedia) (Doctoral Dissertation). University of British Columbia.https://doi.org/10.14288/1.0066802
  • Burke, C. C., Wildung, M. R., & Croteau, R. (1999). Geranyl diphosphate synthase: Cloning, expression, and characterization of this prenyltransferase as a heterodimer. Proceedings of the National Academy of Sciences of the United States of America, 96(23), 13062–13067. [CrossRef]
  • Crowell, A. L., Williams, D. C., Davis, E. M., Wildung, M. R., & Croteau, R. (2002). Molecular cloning and characterization of a new linalool synthase. Archives of Biochemistry and biophysics, 405(1), 112–121. [CrossRef]
  • D’Auria, J. C. (2006). Acyltransferases in plants: A good time to be BAHD. Current Opinion in Plant Biology, 9(3), 331–340. [CrossRef]
  • Demissie, Z. A. (2014). Cloning and functional characterization of genes involved in the biosynthesis and secretion of essential oil constituents of Lavandula (Doctoral Dissertation). University of British Columbia. https://doi.org./10.14288/1.0074341
  • Dudareva, N., Cseke, L., Blanc, V. M., & Pichersky, E. (1996). Evolution of floral scent in Clarkia: Novel patterns of S-linalool synthase gene expression in the C. breweri flower. Plant Cell, 8(7), 1137–1148. [CrossRef]
  • Gang, D. R., Wang, J., Dudareva, N., Nam, K. H., Simon, J. E., Lewinsohn, E., & Pichersky, E. (2001). An investigation of the storage and biosynthesis of phenylpropenes in sweet basil. Plant Physiology, 125(2), 539–555. [CrossRef]
  • Gershenzon, J., McCaskill, D., Rajaonarivony, J. I., Mihaliak, C., Karp, F., & Croteau, R. (1992). Isolation of secretory cells from plant glandular trichomes and their use in biosynthetic studies of monoterpenes and other gland products. Analytical Biochemistry, 200(1), 130–138. [CrossRef]
  • Guenther, E. (1952). The essential oils (Vol. 5). Robert E. (pp. 3-38) Krieger publishing company. Hall, T. (2004). BioEdit version 7.0. 0. Distributed by the Author. [CrossRef]
  • Accessed June 13, 2020.Hsiao, Y. Y., Jeng, M. F., Tsai, W. C., Chuang, Y. C., Li, C. Y., Wu, T. S., Kuoh, C. S., Chen, W. H., & Chen, H. H. (2008). A novel homodimeric geranyl diphosphate synthase from the orchid Phalaenopsis bellina lacking a DD (X) 2–4D motif. Plant Journal, 55(5), 719–733.
  • Jeanmougin, F., Thompson, J. D., Gouy, M., Higgins, D. G., & Gibson, T. J. (1998). Multiple sequence alignment with Clustal X. Trends in Biochemical Sciences, 23(10), 403–405. [CrossRef]
  • Jones, S., & Thornton, J. M. (1996). Principles of protein-protein interactions. Proceedings of the National Academy of Sciences of the United States of America, 93(1), 13–20. [CrossRef]
  • Kamran, H. M., Hussain, S. B., Junzhong, S., Xiang, L., & Chen, L. Q. (2020). Identification and molecular characterization of geranyl diphosphate synthase (GPPS) genes in wintersweet flower. Plants, 9(5), 666. [CrossRef] Kellogg, B. A., & Poulter, C. D. (1997). Chain elongation in the isoprenoid biosynthetic pathway. Current Opinion in Chemical Biology, 1(4), 570–578. [CrossRef]
  • Kıvrak, Ş. (2018). Essential oil composition and antioxidant activities of eight cultivars of Lavender and Lavandin from western Anatolia. Industrial Crops and Products, 117, 88–96. [CrossRef] Lafhal, S., Vanloot, P., Bombarda, I., Kister, J., & Dupuy, N. (2016). Identification of metabolomic markers of lavender and lavandin essential oils using mid-infrared spectroscopy. Vibrational Spectroscopy, 85, 79–90. [CrossRef]
  • Lane, A., Boecklemann, A., Woronuk, G. N., Sarker, L., & Mahmoud, S. S. (2010). A genomics resource for investigating regulation of essential oil production in Lavandula angustifolia. Planta, 231(4), 835–845. [CrossRef]
  • Lin, Y. L., Lee, Y. R., Huang, W. K., Chang, S. T., & Chu, F. H. (2014). Characterization of S-(+)-linalool synthase from several provenances of Cinnamomum osmophloeum. Tree Genetics and Genomes, 10(1), 75–86.[CrossRef]
  • Magnard, J. L., Bony, A. R., Bettini, F., Campanaro, A., Blerot, B., Baudino, S., & Jullien, F. (2018). Linalool and linalool nerolidol synthases in roses, several genes for little scent. Plant physiology and Biochemistry, 127, 74–87. [CrossRef]
  • Muñoz-Bertomeu, J., Arrillaga, I., Ros, R., & Segura, J. (2006). Upregulation of 1-deoxy-D-xylulose-5-phosphate synthase enhances production of essential oils in transgenic spike lavender. Plant Physiology, 142(3), 890–900. [CrossRef]
  • Renaud, E. N. C., Charles, D. J., & Simon, J. E. (2001). Essential oil quantity and composition from 10 cultivars of organically grown lavender and lavandin. Journal of Essential Oil research, 13(4), 269–273. [CrossRef]
  • Schmittgen, T. D., & Livak, K. J. (2008). Analyzing real-time PCR data by the comparative C(T) method. Nature Protocols, 3(6), 1101–1108. [CrossRef] Sarker, L. S., Galata, M., Demissie, Z. A., & Mahmoud, S. S. (2012). Molecular cloning and functional characterization of borneol dehydrogenase from the glandular trichomes of Lavandula x intermedia. Archives of Biochemistry and Biophysics, 528(2), 163–170. [CrossRef]
  • Sarker, L. S. (2013). Cloning of Lavandula essential oil biosynthetic genes(Doctoral Dissertation). University of British Columbia. https://dx.doi.org/10.14288/1.0073585
  • Schilmiller, A. L., Schauvinhold, I., Larson, M., Xu, R., Charbonneau, A. L., Schmidt, A., Wilkerson, C., Last, R. L., & Pichersky, E. (2009). Monoterpenes in the glandular trichomes of tomato are synthesized from a neryl diphosphate precursor rather than geranyl diphosphate. Proceedings of the National Academy of Sciences of the United States of America, 106(26), 10865–10870. [CrossRef]
  • Strommer, J., Gregerson, R., & Vayda, M. (1993). Isolation and characterization of plant mRNA. Methods in Plant Molecular Biology and Biotechnology, 49–65, Boca Raton, FL, USA: CRC
  • Sugiura, M., Ito, S., Saito, Y., Niwa, Y., Koltunow, A. M., Sugimoto, O., & Sakai, H. (2011). Molecular cloning and characterization of a linalool synthase from lemon myrtle. Bioscience, Biotechnology, and Biochemistry, 75(7), 1245–1248. [CrossRef]
  • Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30(12), 2725–2729. [CrossRef] Tholl, D., & Lee, S. (2011). Terpene specialized metabolism in Arabidopsis thaliana. Arabidopsis Book, 9, e0143. [CrossRef]
  • Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994). Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22(22), 4673–4680. [CrossRef]
  • Tucker, A. O. (1985). Lavender, spike, and lavandin. Herbarist, 51, 44–50.
  • Unno, H., Ichimaida, F., Suzuki, H., Takahashi, S., Tanaka, Y., Saito, A., Nishino, T., Kusunoki, M., & Nakayama, T. (2007). Structural and mutational studies of anthocyanin malonyl transferases establish the features of BAHD enzyme catalysis. Journal of Biological Chemistry, 282(21), 15812–15822. [CrossRef]
  • Upson, T., Andrews, S., Harriott, G., King, C., & Langhorne, J. (2004). The genus Lavandula (pp. 442). Timber Press.
  • van Schie, C. C., Haring, M. A., & Schuurink, R. C. (2007). Tomato linalool synthase is induced in trichomes by jasmonic acid. Plant Molecular Biology, 64(3), 251–263. [CrossRef]
  • Wang, G., & Dixon, R. A. (2009). Heterodimeric geranyl(geranyl)diphosphate synthase from hop (Humulus lupulus) and the evolution of monoterpene biosynthesis. Proceedings of the National Academy of Sciences of the United States of America, 106(24), 9914–9919. [CrossRef]
  • Weiss, E. A. (1997). Essential oil crops (pp. 600). CAB International. Whittington, D. A., Wise, M. L., Urbansky, M., Coates, R. M., Croteau, R. B., & Christianson, D. W. (2002). Bornyl iphosphate synthase: Structure and strategy for carbocation manipulation by a terpenoid cyclase. Proceedings of the National Academy of Sciences of the United States of America, 99(24), 15375–15380. [CrossRef]
  • Wu, T., Wang, Y., & Guo, D. (2012). Investigation of glandular trichome proteins in Artemisia annua L. using comparative proteomics. PLoS One, 7(8), e41822. [CrossRef]
  • Zaks, A., Davidovich-Rikanati, R., Bar, E., Inbar, M., & Lewinsohn, E. (2008). Biosynthesis of linalyl acetate and other terpenes in lemon mint (Mentha aquatica var. citrata, Lamiaceae) glandular trichomes. Israel Journal of Plant Sciences, 56(3), 233–244. [CrossRef]
Toplam 39 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Ziraat Mühendisliği (Diğer)
Bölüm Araştırma Makalesi
Yazarlar

Damla Önder 0000-0002-6639-3818

Sercan Önder 0000-0002-8065-288X

Yaşar Karakurt 0000-0003-3914-0652

Yayımlanma Tarihi 19 Ekim 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 54 Sayı: 3 - Research in Agricultural Sciences

Kaynak Göster

APA Önder, D., Önder, S., & Karakurt, Y. (2023). Identification and Molecular Characterization of Genes Related to Terpene Biosynthesis From Lavandula × intermedia. Research in Agricultural Sciences, 54(3), 103-112. https://doi.org/10.5152/AUAF.2023.23150
 Kapak Resmi İndir

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