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Effects of Melatonin on Morus nigra cv. 'Eksi Kara' Exposed to Drought Stress

Yıl 2022, Cilt: 28 Sayı: 4, 555 - 569, 17.10.2022

Duygu Özelçi Gülçin Beker Akbulut Emel Yiğit

https://doi.org/10.15832/ankutbd.953558
Cited By: 1

Öz

Today, drought stress threatens the world seriously. Determining the effects of some exogenous stimulators in acquiring resistance against stress will contribute to agriculture under drought stress. In this regard, we investigated the effects of melatonin (MEL) on Morus nigra cv. 'Eksi Kara' (black mulberry) in challenging drought. To reach this object, we reproduced 'Eksi Kara', which is registered in Turkey and has economic importance, in tissue culture by using the meristem culture method. Plants were then transferred in a medium containing polyethylene glycol (PEG) 8000, which causes -1.5 MPa drought stress, and 20 μl MEL has applied. Leaf samples were taken on the 5th, 10th, and 15th days after treatments in groups of plants grown in a different medium (Control, Control+MEL, PEG and PEG+MEL). The changes in the pigment system, relative water content (RWC) and antioxidant system were evaluated comparatively between the groups to assess plants' growth and determine their roles in coping with stress. Our findings showed that RWC decreased in leaves under drought. Exogenous MEL added in MS medium had a mitigation effect on stress. The reduction was detected in the chlorophyll and carotenoid content of leaves. Moreover, MEL+PEG combination improved the chlorophyll level. It was seen that exogenous MEL application promoted the plant defence mechanism of M. nigra plants, which exposed to drought stress, by increasing the accumulation of non-enzymatic antioxidants; total glutathione (GSH), total phenolic, proline) and activities of antioxidant enzymes; catalase (CAT), superoxide dismutase (SOD), Glutathione-S-transferase (GST), glutathione reductase (GR), peroxidase (POD), ascorbate peroxidase (APX). This study also indicates that the application of MEL+PEG composition partially prevented membrane lipid peroxidation by decreasing (malondialdehyde) MDA content.

Anahtar Kelimeler

Black mulberry Malondialdehyde Pigment Proline Relative water Content Total phenolic

Destekleyen Kurum

İNÖNÜ ÜNİVERSİTESİ

Proje Numarası

Project No FDK-2017-682

Teşekkür

We want to thank İnönü University Scientific Research Project Unit (Project No FDK-2017-682) for supporting the research, and Apricot Research Institute for their contribution in providing laboratory facilities and samples.

Kaynakça

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  • Akerboom T P & Sies H (1981). Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples. In Methods in enzymology 77: 373-382. Academic Press.
  • Andrews C J, Cummins I, Skipsey M, Grundy N M, Jepson I, Townson J& Edwards R, (2005). Purification and characterisation of a family of glutathione transferases with roles in herbicide detoxification in soybean (Glycine max L.); selective enhancement by herbicides and herbicide safeners. Pesticide Biochemistry and Physiology 82(3): 205-219.
  • Arıcı E Ş & Eraslan F (2012). In vitro koşullarda kültüre alınan colt (Prunus avium X Prunus psudocerasus) kiraz anacına tuz stresinin etkisi. Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi, 7 (2):41-48.
  • Arnao M B & Hernández-Ruiz J (2019). Melatonin: a new plant hormone and/or a plant master regulator?. Trends in Plant Science 24(1): 38-48.
  • Arnao M B & Hernández-Ruiz J (2020). Is phytomelatonin a new plant hormone?. Agronomy 10(1): 95.
  • Bates L S, Waldren, R P & Teare I D (1973). Rapid determination of free proline for water-stress studies. Plant and Soil 39(1): 205-207.
  • Blum A & Ebercon A (1981). Cell membrane siability as a measure of drought and heat tolerance in wheat. Crop Sci 2l:43-47.
  • Bolat I, Dikilitas M, Ercisli S, Ikinci A & Tonkaz, T (2014). The effect of water stress on some morphological, physiological, and biochemical characteristics and bud success on apple and quince rootstocks. The Scientific World Journal.
  • Bradford M M (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72(1-2): 248-254.
  • Campos C N, ÁvilaRG de Souza, K R D, Azevedo L M & Alves J D (2019). Melatonin reduces oxidative stress and promotes drought tolerance in young Coffea arabica L. plants. Agricultural Water Management 211: 37-47.
  • Carlberg I & Mannervik B (1985) Glutathione reductase. In Methods in Enzymology, 113: 484-490. Academic press.
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  • Chandler S F & Dodds J H (1983). The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasodine in callus cultures of Solanum laciniatum. Plant Cell Reports, 2(4): 105–108.
  • Chen J, Wu W, Zheng Y, Hou K, Xu Y & Zai J (2010). Drought resistance of Angelica dahurica during seedling stage under polyethylene glycol (PEG-6000)-simulated drought stress. Zhongguo Zhong yao za zhi= Zhongguo Zhongyao Zazhi= China Journal of Chinese Materia Medica, 35(2): 149-153.
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Yıl 2022, Cilt: 28 Sayı: 4, 555 - 569, 17.10.2022

Duygu Özelçi Gülçin Beker Akbulut Emel Yiğit

https://doi.org/10.15832/ankutbd.953558
Cited By: 1

Öz

Proje Numarası

Project No FDK-2017-682

Kaynakça

  • Ahammed G J, Wang Y, Mao Q, Wu M, Yan Y, Ren J, Wang X, Liu A & Chen S (2020a). Dopamine alleviates bisphenol A-induced phytotoxicity by enhancing antioxidant and detoxification potential in cucumber. Environmental Pollution 259: 113957.
  • Akerboom T P & Sies H (1981). Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples. In Methods in enzymology 77: 373-382. Academic Press.
  • Andrews C J, Cummins I, Skipsey M, Grundy N M, Jepson I, Townson J& Edwards R, (2005). Purification and characterisation of a family of glutathione transferases with roles in herbicide detoxification in soybean (Glycine max L.); selective enhancement by herbicides and herbicide safeners. Pesticide Biochemistry and Physiology 82(3): 205-219.
  • Arıcı E Ş & Eraslan F (2012). In vitro koşullarda kültüre alınan colt (Prunus avium X Prunus psudocerasus) kiraz anacına tuz stresinin etkisi. Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi, 7 (2):41-48.
  • Arnao M B & Hernández-Ruiz J (2019). Melatonin: a new plant hormone and/or a plant master regulator?. Trends in Plant Science 24(1): 38-48.
  • Arnao M B & Hernández-Ruiz J (2020). Is phytomelatonin a new plant hormone?. Agronomy 10(1): 95.
  • Bates L S, Waldren, R P & Teare I D (1973). Rapid determination of free proline for water-stress studies. Plant and Soil 39(1): 205-207.
  • Blum A & Ebercon A (1981). Cell membrane siability as a measure of drought and heat tolerance in wheat. Crop Sci 2l:43-47.
  • Bolat I, Dikilitas M, Ercisli S, Ikinci A & Tonkaz, T (2014). The effect of water stress on some morphological, physiological, and biochemical characteristics and bud success on apple and quince rootstocks. The Scientific World Journal.
  • Bradford M M (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72(1-2): 248-254.
  • Campos C N, ÁvilaRG de Souza, K R D, Azevedo L M & Alves J D (2019). Melatonin reduces oxidative stress and promotes drought tolerance in young Coffea arabica L. plants. Agricultural Water Management 211: 37-47.
  • Carlberg I & Mannervik B (1985) Glutathione reductase. In Methods in Enzymology, 113: 484-490. Academic press.
  • Caruso A, Chefdor F, Carpin S, Depierreux C, Delmotte F M, Kahlem G & Morabito D, (2008). Physiological characterization and identification of genes differentially expressed in response to drought induced by PEG 6000 in Populus canadensis leaves. Journal of Plant Physiology 165(9): 932-941.
  • Chandler S F & Dodds J H (1983). The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasodine in callus cultures of Solanum laciniatum. Plant Cell Reports, 2(4): 105–108.
  • Chen J, Wu W, Zheng Y, Hou K, Xu Y & Zai J (2010). Drought resistance of Angelica dahurica during seedling stage under polyethylene glycol (PEG-6000)-simulated drought stress. Zhongguo Zhong yao za zhi= Zhongguo Zhongyao Zazhi= China Journal of Chinese Materia Medica, 35(2): 149-153.
  • Chen Y, Chen F.; Liu L, Zhu S (2012). Physiological responses of Leucaena leucocephala seedlings to drought stress. Procedia Engineering 28: 110-116.
  • Cheng L, Han M, Yang L M, Li Y, Sun Z &Zhang T (2018). Changes in the physiological characteristics and baicalin biosynthesis metabolism of Scutellaria baicalensis Georgi under drought stress. Industrial Crops and Products 122: 473-482.
  • Collins A R (2001). Carotenoids and genomic stability. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 475(1-2): 21-28.
  • Cakmak I (1994). Activity of ascorbate-dependent H2O2-scavenging enzymes and leaf chlorosis are enhanced in magnesium-and potassium-deficient leaves, but not in phosphorus-deficient leaves. Journal of Experimental Botany, 45(9): 1259-1266.
  • Datta R K (2002). Mulberry cultivation and utilization in India. Mulberry for animal production. FAO Animal Production and Health Paper, 147: 45-62.
  • De Kok L J & Graham M (1980). Levels of pigments, soluble proteins, amino acids and sulhydryl compounds in foliar tissue of Arabidopsis thaliana during dark-induced and natural senescence. Plant Physiology and Biochemistry (Paris) 27(2): 133-142.
  • Demiral T & Turkan I (2005). Comparative lipid peroxidation, antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance. Environmental and Experimental Botany 53(3): 247-257.
  • Ding F, Liu B & Zhang S (2017). Exogenous melatonin ameliorates cold-induced damage in tomato plants. Scientia Horticulturae, 219: 264-271. Duncan D B (1955). Multiple range and multiple F tests biometrics. International Biometric Society, 11(1): 1–42.
  • Fracheboud Y, Jompuk C, Ribaut J M, Stamp J & Leipner P (2004). Genetic analysis of cold-tolerance of photosynthesis in maize. Plant Molecular Biology 56(2): 241-253.
  • Gao S, Wang Y, Yu S, Huang Y, Liu H, Chen W & He X (2020). Effects of drought stress on growth, physiology and secondary metabolites of two Adonis species in Northeast China. Scientia Horticulturae, 259, 108795.
  • Gill S S &Tuteja N (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry 48(12): 909-930.
  • Guller P, Karaman M, Guller U, Aksoy M & Kufrevioglu OI (2020). A study on the effects of inhibition mechanism of curcumin, quercetin, and resveratrol on human glutathione reductase through in vitro and in silico approaches. Journal of Biomolecular Structure and Dynamics 1-10.
  • Habig W H, Pabst M J & Jakoby W B (1974). The first enzymatic step in mercapturic acid formation Glutathion S-Transferases. J. Biol. Chem 249: 7130-7139.
  • Han Q H, Huang B, Ding C B, Zhang Z W, Chen Y E, Hu C, Zhou L, Huang Y, Liao J, Yuan S & Yuan M (2017). Effects of melatonin on anti-oxidative systems and photosystem II in cold-stressed rice seedlings. Frontiers in Plant Science 8: 785.
  • Heath R L & Packer L (1968). Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125(1): 189-198.
  • Huihui Z, Xin L, Yupeng G, Mabo L, Yue W, Meijun A, Yuehui Z, Guanjun L, Nan X & Guangyu S (2020). Physiological and proteomic responses of reactive oxygen species metabolism and antioxidant machinery in mulberry (Morus alba L.) seedling leaves to NaCl and NaHCO3 stress. Ecotoxicology and Environmental Safety, 193: 110259.
  • Ipek M (2015). In vitro şartlarda Garnem ve Myrobolan 29C anaçlarının kurak stresine karşı tepkilerinin belirlenmesi. Doktora Tezi, Selçuk Üniversitesi, Fen Bilimleri Enstitüsü, Konya.
  • Jacoby R P, Millar A H, Taylor N L (2010). Wheat mitochondrial proteomes provide new links between antioxidant defense and plant salinity tolerance. Journal of Proteome Research 9(12): 6595-6604.
  • Jahan M S, Shu S, Wang Y, Chen Z, He M, Tao M, Sun J & Guo S (2019). Melatonin alleviates heat-induced damage of tomato seedlings by balancing redox homeostasis and modulating polyamine and nitric oxide biosynthesis. BMC Plant Biology, 19(1): 1-16.
  • Jain M, Mathur G, Koul S & Sarin N (2001). Ameliorative effects of proline on salt stress-induced lipid peroxidation in cell lines of groundnut (Arachis hypogaea L.). Plant Cell Reports 20(5): 463-468.
  • Ju Y L, Yue X F, Zhao X F, Zhao H, Fang Y L (2018). Physiological, micro-morphological and metabolomic analysis of grapevine (Vitis vinifera L.) leaf of plants under water stress. Plant Physiology and Biochemistry 130: 501-510.
  • Karimi S, Yadollahi A, Nazari-Moghadam R, Imani A, Arzani K (2012). In vitro screening of almond (Prunus dulcis (Mill.)) genotypes for drought tolerance. J Biol Environ Sci 6(18): 263-270.
  • Karuppanapandian T, Moon J C, Kim C, Manoharan K & Kim W (2011). Reactive oxygen species in plants: their generation, signal transduction, and scavenging mechanisms. Australian Journal of Crop Science 5(6): 709.
  • Kaya A & Doganlar Z B (2019) Melatonin improves the multiple stress tolerance in pepper (Capsicum annuum). Scientia Horticulturae 256: 108509. Kocacalıskan İ (2008). Bitki Fizyolojisi. Nobel Yayın Dağıtım, Fen ve Biyoloji Yayınları Dizisi, 316.
  • Korkmaz A, Demir O, Kocaçınar F, Cuci Y (2016). Biber fidelerinde yapraktan yapılan melatonin uygulamalarıyla üşüme stresine karşı toleransın arttırılması. Tarım ve Doğa Dergisi 19(3): 348-354.
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Toplam 80 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Mühendislik
Bölüm Makaleler
Yazarlar

Duygu Özelçi 0000-0003-1621-1980

Gülçin Beker Akbulut 0000-0002-4964-6780

Emel Yiğit 0000-0001-6333-8437

Proje Numarası Project No FDK-2017-682
Yayımlanma Tarihi 17 Ekim 2022
Gönderilme Tarihi 17 Haziran 2021
Kabul Tarihi 18 Ekim 2021
Yayımlandığı Sayı Yıl 2022 Cilt: 28 Sayı: 4

Kaynak Göster

APA Özelçi, D., Beker Akbulut, G., & Yiğit, E. (2022). Effects of Melatonin on Morus nigra cv. ’Eksi Kara’ Exposed to Drought Stress. Journal of Agricultural Sciences, 28(4), 555-569. https://doi.org/10.15832/ankutbd.953558

Cited By

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Frontiers in Plant Science
https://doi.org/10.3389/fpls.2023.1280794
 Kapak Resmi İndir

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