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Antifungal effect of carbonate and bicarbonate salts against Botrytis cinerea, the casual agent of grey mould of kiwifruit

Yıl 2017, Cilt: 6 Sayı: 2, 107 - 114, 30.12.2017
https://doi.org/10.29278/azd.371066

Öz

Control of grey mould of kiwifruit caused by Botrytis cinerea has been accomplished by postharvest application of synthetic fungicides. However, the development of resistant fungal strains and increasing public concern over food safety and the environment are driving a search for alternative disease control strategies. In the present study, the inhibitory effect of carbonate and bicarbonate salts of ammonium, potassium and sodium against B. cinerea were investigated in both in vitro and in vivo experiments. Ammonium carbonate, ammonium bicarbonate, sodium carbonate, sodium bicarbonate, potassium carbonate and potassium bicarbonate completely inhibited the mycelial growth of B. cinerea at 10, 25, 25, 50, 50 and 75 mM, respectively. With the exceptions of few, carbonate and bicarbonate salts totaly halted spore germination at lower concentrations than that of the mycelial growth of fungus. Complete inhibitory activity of ammonium carbonate exhibited spore germination at 10 mM, whereas same concentration of sodium carbonate reduced spore germination of fungus by 98.75%; however, the difference between this and the effects of first salt was not statistically significant (P<0.05). The lowest minimum inhibition concentration (MIC) and EC50 values were also recorded in ammonium carbonate treatment. In vivo, however, with the exception of 100 mM ammonium carbonate, five other carbonate and bicarbonate salts significantly reduced the incidence of grey mould on kiwifruits (cv. Hayward). Moreover, potassium bicarbonate was detected to be the most effective salt for in vivo control of disease, and the difference between the effects of the lowest and highest concentrations of the salt was not statistically significant (P<0.05). Results from this study may provide an important basis for further study on the uses of carbonate and bicarbonate salts in the control of grey mould in kiwifruit at wider semi-commercial conditions.

Kaynakça

  • Aharoni, Y., E. Fallik, A. Copel, M. Gil, S. Grinberg, J. D. Klein. 1997. Sodium bicarbonate reduces postharvest decay development on melons. Postharvest Biol. Technol. 10:201-206.
  • Anonymous, 2016. U.S. Food and Drug Administration (FDA) http://www.fda.gov/Food/Ingredients PackagingLabeling/GRAS/ (verified May 06, 2016).
  • Arslan, U., Kadir, I., Vardar, C., and Karabulut, O. A. 2009. Evaluation of antifungal activity of food additives against soilborne phytopathogenic fungi. World Journal of Microbiology and Biotechnology 25: 537–543.
  • Bombelli E. C. and Wright E. R. 2006. Tomato fruit quality conservation during post-harvest by application of potassium bicarbonate and its effect on Botrytis cinerea. Cien. Inv. Agr. 33(3): 167-172.
  • Brook, P. J. 1986. Diseases of kiwifruit. In ‘Kiwifruit: science and management’. (Eds IJ Warrington, GC Weston). New Zealand, Ray Richards Publisher, 420–428.
  • Campanella, V., Ippolito, A., and Nigro, F. 2002. Activity of calcium salts in controlling Phytophthora root rot of citrus. Crop Protection 21: 751–756.
  • Delen, N. 2016. Fungicides. Nobel Academic Publishing, Second Edt., Ankara, 552p.
  • DePasquale, D. A., El-Nabarawy, A., Rosen, J. D., Montville, T. J. 1990a. Ammonium bicarbonate inhibition of mycotoxigenic fungi and spoilage yeasts. Journal of Food Protection 53: 324-328.
  • Depasquale, D. A., Montville, T. J., 1990b. Mechanism by which ammonium bicarbonate and ammonium sulfate inhibit mycotoxigenic fungi. Appl. Environ. Microbial. 56:3711-3717.
  • Droby, S., Wisniewski, M. E., El Ghaouth, A., Wilson, C. 2003. Influence of food additives on the control of postharvest rots of apple and peach and efficacy of the yeast-based biocontrol product Aspire. Postharvest Biology and Technology 27: 127-135.
  • Erper, İ, Türkkan, M., Karaca, G.H., Kılıç, G., 2011. Evaluation of in vitro antifungal activity of potassium bicarbonate on Rhizoctonia solani AG 4 HG-I, Sclerotinia sclerotiorum and Trichoderma sp. African Journal of Biotechnology Vol. 10 (43): 8605-8612.
  • Fallik, E., S. Grinberg, O. Ziv, O., 1997. Potassium bicarbonate reduces postharvest decay development on bell pepper fruits. J. Hort. Sci. 72:35-41.
  • Fan C.M., Xıong G. R., Qi P., Ji G. H. He Y. Q. 2008. Potential biofumigation effects of Brassica oleracea var. caulorapa on growth of fungi. Journal of Phytopathology, 156:321-325.
  • FAO, 2016. Food and agriculture organization. The database of annual production. FAOSTAT. Statistical database. http:// faostat.fao.org. (verified December 10, 2016).
  • Koh Y. J., Jung J. S., Hur J. S., 2003. Current status of occurrence of major diseases on kiwifruits and their control in Korea. Acta Horticulturae 610: 437-443.
  • Koh Y. J., Hur, J-S, and Jung, J. S., 2005. Postharvest fruit rots of kiwifruit (Actinidia deliciosa) in Korea. New Zealand Journal of Crop and Horticultural Science 33: 303-310.
  • Latifa A., Idriss T., Hassan B., Amine S. M., El Hassane B. and Abdellah A. B. A., 2011. Effects of organic acids and salts on the development of Penicillium italicum: the causal agent of citrus blue mold. Plant Pathol Journal 10: 99-107.
  • Mecteau M. R., Arul J. and Tweddell R. J. 2002. Effect of organic and inorganic salts on the growth and development of Fusarium sambucinum, a causal agent of potato dry rot. Mycol Res 106:688-696.
  • Moorman G. W. and Lease, R. J. 1992. Benzimidazole- and dicarboximide-resistant Botrytis cinerea from Pennsylvania greenhouses. Plant Dis. 76:477-480.
  • Northover J., Matteoni J. A., 1986. Resistance of Botrytis cinerea to benomyl and iprodione in vineyards and greenhouses after exposure to the fungicides alone or mixed with captan. Plant Disease 70: 398-402.
  • Nunes C., Usall J., Teixido N, de Eribe X. O., Vinas I. 2001. Control of post-harvest decay of apples by pre-harvest and post-harvest application of ammonium molybdate. Pest Management Sci. 57: 1093-1099.
  • Olivier C., Halseth D.E., Mizubuti E.S.G., Loria R. 1998. Postharvest application of organic and inorganic salts for suppression of silver scurf on potato tubers. Plant Disease 82:213-217.
  • Palmer C. L., Horst R. K., Langhans R.W. 1997. Use of bicarbonates to inhibit in vitro colony growth of Botrytis cinerea. Plant Disease 81:1432-1438.
  • Palou, L., Smilanick, J. L., Usall, J., Viñas, I., 2001. Control of postharvest blue and green molds of oranges by hot water, sodium carbonate, and sodium bicarbonate. Plant Dis. 85: 371-376.
  • Palou, L., Usall, J., Smilanick, J.L., Aguilar, M-J., Vinas, I. 2002. Evaluation of food additives and low-toxicity compounds as alternative chemicals for the control of Penicillium digitatum and Penicillium italicum on citrus fruit. Pest Manag Sci 58: 459–466.
  • Pennycook S. R. 1985. Fungal fruit rots of Actinidia deliciosa (kiwifruit). New Zealand Journal of Experimental Agriculture 13: 289-299.
  • Punja, Z. K. and Grogan, R. G., 1982. Effects of inorganic salts, carbonate-bicarbonate anions, ammonia, and the modifying influence of pH on sclerotial germination of Sclerotium rolfsii. Phytopathology 72:635-639.
  • Punja, Z. K., Gaye, M. M. 1993. Influence of postharvest handling practices and dip treatments on development of black root rot on fresh market carrots. Plant Dis 77:989–995.
  • Sommer, N. 1985. Role of controlled environments in suppression of postharvest diseases. Canadian Journal of Plant Pathology 7: 331.
  • Soylu, E. M., Kurt, Ş., Soylu, S. 2010. In vitro and in vivo antifungal activities of the essential oils of various plants against tomato grey mould disease agent Botrytis cinerea. International Journal of Food Microbiology 143:183-189.
  • Ten A., Mulder W., Visser J., van Kan J. A. 1998. The endopolygalacturonase gene Bcpg1 is required for full virulence of Botrytis cinerea. Mol Plant Microbe Interact 11 1009-1016.
  • TUİK, 2016. Agricultural Production Statistics of Turkish Statistical Institute. http://www.tuik.gov.tr/ bitkiselapp/bitkisel.zul (verified December 10, 2016).
  • Türkkan, M., Erper, İ., 2014. Multi Regression Analysis of The Effect of Potassium Bicarbonate on In Vitro The Mycelial Growth and Sclerotial Germination of Botrytis cinerea. Akademik Ziraat Dergisi 3(2): 53-60.
  • Türkkan, M., 2015. Evaluation of Inhibitory Effect of Organic and Inorganic Salts Against Ilyonectria liriodendri, The Causal Agent of Root Rot Disease of Kiwifruit. Journal of Phytopathology 163(7-8): 567-577.
  • Wisniewski, M. E, Droby, S., El-Ghaouth, A. Wilson, C. L, 1998. The use of food additives to control postharvest decay and enhance biocontrol activity of yeast antagonist, in Proc Internat Congress Plant pathol, August 9-16, Edinburg, Scotlant, (Abstract 5.2.61).
  • Youssef, K., Ligorio, A., Sanzani, S. M., Nigro, F., Ippolito, A. 2012. Control of storage diseases of citrus by pre- and postharvest application of salts. Postharvest Biol Technol 72: 57–63.
  • Ziv, O., Zitter, T. A. 1992. Effects of bicarbonate and film-forming polymers on cucurbit foliar diseases. Plant Disease 76:513–517.
Toplam 37 adet kaynakça vardır.

Ayrıntılar

Bölüm Makaleler
Yazarlar

Muharrem Türkkan

Muharrem Özcan Bu kişi benim

İsmail Erper Bu kişi benim

Yayımlanma Tarihi 30 Aralık 2017
Yayımlandığı Sayı Yıl 2017 Cilt: 6 Sayı: 2

Kaynak Göster

APA Türkkan, M., Özcan, M., & Erper, İ. (2017). Antifungal effect of carbonate and bicarbonate salts against Botrytis cinerea, the casual agent of grey mould of kiwifruit. Akademik Ziraat Dergisi, 6(2), 107-114. https://doi.org/10.29278/azd.371066

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