Araştırma Makalesi
Momordica charantia L. (Kudret narı) meyvelerinin toplam fenolik madde içerikleri ve antioksidan kapasitelerinin değerlendirilmesi
Öz
Bu çalışmada, Momordica charantia L. (Cucurbitaceae) bitkisinin ülkemizin farklı yerlerinden
toplanmış 13 farklı genotipine ait ham ve olgun meyveler kullanılmıştır.
Genotipler, saflaştırmak için iki yıl süreyle arazide kendileme çalışmalarına
devam edilerek F3 kademesine getirilmiştir. Farklı genotiplerin ham ve olgun
meyvelerinin pulp kısımları ayrılarak, bu kısımlardan Sokslet ekstraksiyonu ile
%70’lik metanol ekstreleri hazırlanmıştır. Ekstrelerin
2,2-difenil-1-pikrihidrazil (DPPH•) radikali süpürücü etkileri ve
toplam fenolik madde içerikleri tespit edilmiştir. Sonuçta M. charantia meyveleri için 3, 8 ve 9 nolu genotiplerin ümitvar
oldukları tespit edilmiştir.
Anahtar Kelimeler
Momordica charantia Genotip DPPH• Toplam fenolik madde içeriği
Kaynakça
- Ahmed, I., Lakhani, M.S., Gillett, M., John, A., & Raza, H. (2001). Hypotriglyceridemic and hypocholesterolemic effects of anti-diabetic Momordica charantia (karela) fruit extract in streptozotocin-induced diabetic rats. Diabetes Research Clinical Practices 51:155-161.
- Bachmayer, O. (2004). Antioxidant Properties of Aqueous Extracts from Selected Culinary Herbs. Yüksek Lisans Tezi, University of Helsinki, Helsinki, Finland.
- Basch, E., Gabardi, S., & Ulbricht, C. (2003). Bitter melon (Momordica charantia): a review of efficacy and safety. American Journal of Health-System Pharmacy, 60(4):356-359.
- Biswas, A.R., Ramaswamy, S., & Bapna, J.S. (1991). Analgesic effect of Momordica charantia seed extract in mice and rats. Journal of Ethnopharmacology, 31:115-118.
- Budrat, P., & Shotipruk, A. (2008). Extraction of phenolic compounds from fruits of bitter melon (Momordica charantia) with subcritical water extraction and antioxidant activities of these extracts. Chiang Mai Journal of Science, 13:123-130.
- F Fang, E., & B Ng, T. (2011). Bitter gourd (Momordica charantia) is a cornucopia of health: a review of its credited antidiabetic, anti-HIV, and antitumor properties. Current molecular medicine, 11(5):417-436.
- Garau, C., Singh, J., & Cummings, E. (2003). Beneficial effect and mechanism of action of Momordica charantia in the treatment of diabetes mellitus: a mini review. International Journal of Diabetes and Metabolism, 11:46-55.
- Grover J.K, & Yadav S.P. (2004). Pharmacological Actions And Potential Uses Of Momordica charantia. Journal of Ethnopharmacology, 93(1):123-132.
- Güner, A., Aslan, S., Ekim, T., Vural M., & Babaç M.T. (2012). Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını: 1, 390 s., İstanbul.
- Gyamfi, M.A., Yonamine, M., & Aniya, Y. (1999). Free-Radical Scavenging Action of Medicinal Herbs from Ghana Thonningia Sanguinea on Experimentally Induced Liver Injuries. General Pharmacology, 32:661-667.
- Horax, R., Hettiarachchy, N., & Kannan, A. (2011). Protein extraction optimisation, characterisation and functionalities of protein isolate from bitter melon (Momordica charantia) seed. Food Chemistry, 124(2):545-550.
- Ilhan, M., Bolat, İ.E., Süntar, İ., Kutluay Köklü, H., Uğar Çankal, D.A., Keleş, H., & Akkol, E.K. (2015). Tropical application of olive oil macerate of Momordica charantia L. promotes healing of excisional and incisional wounds in rat buccal mucosa. Archives of Oral Biology, 60(12):1708-1713.
- Kenny, O., Smyth, T.J., Hewage, C.M., & Brunton, N.P. (2013). Antioxidant properties and quantitative UPLC-MS analysis of phenolic compounds from extracts of fenugreek Trigonella foenum-graecum; seeds and bitter melon Momordica charantia fruit. Food Chemistry, 141(4):4295-4302.
- Leung, L., Birtwhistle, R., Kotecha, J., Hannah, S., & Cuthbertson, S. (2009). Anti-diabetic and hypoglycaemic effects of Momordica charantia (Bitter melon): a mini review. British Journal of Nutrition, 102(12):1703-1708.
- Mesia, G.K., Tona, G.L., Nanga, T.H., Cimanga, R.K., Apers, S., Cos, P., Maes, L., Pieters, L., & Vlietinck, A.J. (2008). Antiprotozoal and cytotoxic screening of 45 plant extracts from Democratic Republic of Congo. Journal of Ethnopharmacology, 115(3):409-415.
- Nagarani, G., Abirami, A., & Siddhuraju, P. (2014). A comparative study on antioxidant potentials, inhibitory activities against key enzymes related to metabolic syndrome, and anti-inflammatoryactivity of leaf extract from different Momordica species. Food Science and Human Wellness, 3:36-46.
- Raman, A., & Lau, C. (1996). Anti-diabetic properties and phytochemistry of Momordica charantia L. (Cucurbitaceae). Phytomedicine, 2:349-362.
- Semiz, A., & Sen, A. (2007). Antioxidant and chemoprotective properties of Momordica charantia L. (bitter melon) fruit extract. African Journal of Biotechnology, 6(3):273-277.
- Singleton, V.L., Orthofer, R., & Lamuela-Ravento´S, R.M. (1999). Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin- Ciocalteu Reagent. pp. 152-315. In: Packer, L. (Ed.), Methods in Enzymology. Academic Press, San Diego, CA.
- Wu, S.-J., & Lean Teik, Ng. (2008). Antioxidant and free radical scavenging activities of wild bitter melon (Momordica charantia Linn. var. abbreviata Ser.) in Taiwan. LWT-Food Science and Technology, 41(2):323-330.
- Zhang, F., Lin, L., & Xie, J. (2016). A mini-review of chemical and biological properties of polysaccharides from Momordica charantia. International Journal of Biological Macromolecules, 92:246-253.
Evaluation of total phenolic contents and antioxidant capacities of Momordica charantia L. (Bitter gourd) fruits
Öz
In
this study, raw and mature fruits of 13 different genotypes of Momordica charantia L. (Cucurbitaceae)
collected from different regions of Turkey were used. F3 seeds of the genotypes
were produced for two years by selfing. Pulp parts of raw and ripe fruits of
different genotypes were separated and 70% methanol extracts were prepared by
Soxhlet extraction from these parts. Total phenolic contents of the extracts
and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities were determined. As a result,
genotypes 3, 8 and 9 for M. charantia
fruit were found to be particularly promising.
Kaynakça
- Ahmed, I., Lakhani, M.S., Gillett, M., John, A., & Raza, H. (2001). Hypotriglyceridemic and hypocholesterolemic effects of anti-diabetic Momordica charantia (karela) fruit extract in streptozotocin-induced diabetic rats. Diabetes Research Clinical Practices 51:155-161.
- Bachmayer, O. (2004). Antioxidant Properties of Aqueous Extracts from Selected Culinary Herbs. Yüksek Lisans Tezi, University of Helsinki, Helsinki, Finland.
- Basch, E., Gabardi, S., & Ulbricht, C. (2003). Bitter melon (Momordica charantia): a review of efficacy and safety. American Journal of Health-System Pharmacy, 60(4):356-359.
- Biswas, A.R., Ramaswamy, S., & Bapna, J.S. (1991). Analgesic effect of Momordica charantia seed extract in mice and rats. Journal of Ethnopharmacology, 31:115-118.
- Budrat, P., & Shotipruk, A. (2008). Extraction of phenolic compounds from fruits of bitter melon (Momordica charantia) with subcritical water extraction and antioxidant activities of these extracts. Chiang Mai Journal of Science, 13:123-130.
- F Fang, E., & B Ng, T. (2011). Bitter gourd (Momordica charantia) is a cornucopia of health: a review of its credited antidiabetic, anti-HIV, and antitumor properties. Current molecular medicine, 11(5):417-436.
- Garau, C., Singh, J., & Cummings, E. (2003). Beneficial effect and mechanism of action of Momordica charantia in the treatment of diabetes mellitus: a mini review. International Journal of Diabetes and Metabolism, 11:46-55.
- Grover J.K, & Yadav S.P. (2004). Pharmacological Actions And Potential Uses Of Momordica charantia. Journal of Ethnopharmacology, 93(1):123-132.
- Güner, A., Aslan, S., Ekim, T., Vural M., & Babaç M.T. (2012). Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını: 1, 390 s., İstanbul.
- Gyamfi, M.A., Yonamine, M., & Aniya, Y. (1999). Free-Radical Scavenging Action of Medicinal Herbs from Ghana Thonningia Sanguinea on Experimentally Induced Liver Injuries. General Pharmacology, 32:661-667.
- Horax, R., Hettiarachchy, N., & Kannan, A. (2011). Protein extraction optimisation, characterisation and functionalities of protein isolate from bitter melon (Momordica charantia) seed. Food Chemistry, 124(2):545-550.
- Ilhan, M., Bolat, İ.E., Süntar, İ., Kutluay Köklü, H., Uğar Çankal, D.A., Keleş, H., & Akkol, E.K. (2015). Tropical application of olive oil macerate of Momordica charantia L. promotes healing of excisional and incisional wounds in rat buccal mucosa. Archives of Oral Biology, 60(12):1708-1713.
- Kenny, O., Smyth, T.J., Hewage, C.M., & Brunton, N.P. (2013). Antioxidant properties and quantitative UPLC-MS analysis of phenolic compounds from extracts of fenugreek Trigonella foenum-graecum; seeds and bitter melon Momordica charantia fruit. Food Chemistry, 141(4):4295-4302.
- Leung, L., Birtwhistle, R., Kotecha, J., Hannah, S., & Cuthbertson, S. (2009). Anti-diabetic and hypoglycaemic effects of Momordica charantia (Bitter melon): a mini review. British Journal of Nutrition, 102(12):1703-1708.
- Mesia, G.K., Tona, G.L., Nanga, T.H., Cimanga, R.K., Apers, S., Cos, P., Maes, L., Pieters, L., & Vlietinck, A.J. (2008). Antiprotozoal and cytotoxic screening of 45 plant extracts from Democratic Republic of Congo. Journal of Ethnopharmacology, 115(3):409-415.
- Nagarani, G., Abirami, A., & Siddhuraju, P. (2014). A comparative study on antioxidant potentials, inhibitory activities against key enzymes related to metabolic syndrome, and anti-inflammatoryactivity of leaf extract from different Momordica species. Food Science and Human Wellness, 3:36-46.
- Raman, A., & Lau, C. (1996). Anti-diabetic properties and phytochemistry of Momordica charantia L. (Cucurbitaceae). Phytomedicine, 2:349-362.
- Semiz, A., & Sen, A. (2007). Antioxidant and chemoprotective properties of Momordica charantia L. (bitter melon) fruit extract. African Journal of Biotechnology, 6(3):273-277.
- Singleton, V.L., Orthofer, R., & Lamuela-Ravento´S, R.M. (1999). Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin- Ciocalteu Reagent. pp. 152-315. In: Packer, L. (Ed.), Methods in Enzymology. Academic Press, San Diego, CA.
- Wu, S.-J., & Lean Teik, Ng. (2008). Antioxidant and free radical scavenging activities of wild bitter melon (Momordica charantia Linn. var. abbreviata Ser.) in Taiwan. LWT-Food Science and Technology, 41(2):323-330.
- Zhang, F., Lin, L., & Xie, J. (2016). A mini-review of chemical and biological properties of polysaccharides from Momordica charantia. International Journal of Biological Macromolecules, 92:246-253.
Toplam 21 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Haziran 2018 |
| Yayımlandığı Sayı | Yıl 2018 Cilt 35 Sayı 1 |
Kaynak Göster
Cited By
Investigation of Antimicrobial Activities of Extracts of Some Plant Sources Prepared in Different Solvents
Çukurova Üniversitesi Mühendislik Fakültesi Dergisi
https://doi.org/10.21605/cukurovaumfd.1146592
DERİM in
/Sharif1/indexingimg/IC.png)
ISSN : 1300-3496
e-ISSN : 2149-2182
Derim Creative Commons Al 4.0 Uluslararası Lisansı ile lisanslanmıştır.
------------------------------------------------------------------
DERİM
Batı Akdeniz Tarımsal Araştırma Enstitüsü
Demircikara Mh. Paşa Kavakları Cad. No:11, P.K.35 Antalya
derim@derim.com.tr
www.derim.com.tr