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Cardaria draba'nın Farklı Kısımlarının Fenolik Bileşimi, İn-Vitro Antioksidan ve Enzim İnhibisyon Aktiviteleri

Yıl 2022, Sayı: 35, 424 - 431, 07.05.2022

Nuraniye Eruygur Fatma Ayaz Yavuz Bağcı Ebru Güler Esra Maltaş Çağıl

https://doi.org/10.31590/ejosat.1062109
Cited By: 1

Öz

Cardaria draba (L.) Desv. subsp. draba (Brassicaceae) bitkisi geleneksel olarak farklı kültürlerde çeşitli rahatsızlıklar için kullanılmaktadır. C. draba'nın çiçek, yaprak, gövde ve köklerinden elde edilen metanol, etanol ve su gibi farklı solvent ekstrelerinin kimyasal bileşimleri ile antioksidan aktivitesi ve asetilkolinesteraz (AChE), butirilkolinesterazın (BChE), α-glukosidaz, α-amilaz ve tirozinaz enzimleri üzerindeki inhibisyon aktivitelerini araştırdık. Her ekstrenin fenolik profilini belirlemek için RP-HPLC tekniği kullanıldı. Ekstrelerin antioksidan aktiviteleri ise DPPH ve ABTS yöntemi, toplam fenol ve flavonoid içerik analizi ve demir şelatlama deneyi kullanılarak belirlendi. Enzim inhibitör potansiyeli 96-mikroplaka yöntemi ile değerlendirildi. Sonuçlara göre, çiçek ekstresi en yüksek toplam fenolik içeriği (64.32 µgGAE/ mg) gösterirken, yaprak etanol ekstresi ise tüm özütler arasında en yüksek toplam flavonoid içeriğine (141.47 µgQE/ mg) sahip olduğu tespit edilmiştir. Çiçeklerin metanol ve su ekstraktları en etkili DPPH süpürücü iken, çiçeklerin sulu ekstraktı ABTS süpürmede en aktifti. Ekstraktların α-glukosidaza karşı α-amilaz enziminden daha umut verici aktiviteye sahip olduğu gösterilmiştir. Çiçeğin metanolik özütü, anti-asetilkolinesteraz aktivitesi gösterdi ve gövdenin etanolik özü, 12 özüt arasında en iyi anti-bütirilkolinesteraz aktivitesi göstermiştir. Anti-tirozinaz aktivitesine gelince, kök ve yaprakların sulu ekstraktları için daha yüksek kojik asite eşdeğer değerleri bulundu. Sonuç olarak, bu çalışmadan elde edilen veriler, aktif bileşiklerin biyo-aktivite rehberli izolasyonu ve Cardaria draba'dan yeni ilaçların geliştirilmesi için temel niteliği taşımaktadır.

Anahtar Kelimeler

Cardaria draba Antioksidan aktivite Enzim inhibisyon YBSK

Kaynakça

  • Askin, H., Yildiz, M., Ayar, A. (2017). Effects of thymol and carvacrol on acetylcholinesterase from Drosophila melanogaster. Acta Phys Pol A, 132(3), 720-722.
  • Ainsworth, E. A., & Gillespie, K. M. (2007). Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. Nature protocols, 2(4), 875-877.
  • Bag, G.C., Grihanjali Devi, P., Bhaigyaba, T. (2015). Assessment of total flavonoid content and antioxidant activity of methanolic rhizome extract of three Hedychium species of Manipur valley. Int J Pharm Sci Rev Res., 30(1),154-159.
  • Boutemak, K., Safta, B., & Ayachi, N. (2015). Study of the Anti-Inflammatory Activity of Flavonic Extract of Globularia alypum L. Acta Physica Polonica A, 128(2B), B-239..
  • Benahmed-Bouhafsoun, A., Djebbar, H., & Kaid-Harche, M. (2015). Determination of Polyphenolic Compounds of Washingtonia robusta H. Wendl Extracts. Acta Physica Polonica A, 128(2B), B-465.
  • Bicha, S., Benmekhebi, L., Boubekri, N., Khellaf, R., Brouard, I., Zama, D., ... & Benayache, F. (2016). Compositional study, antibacterial and antioxidant potential of Lepidium draba L.(Brascicaceae). Res J Pharm Biol Chem Sci., 7(2), 283-287.
  • Ceylan, Y., Usta, K., Usta, A., Maltas, E., Yildiz, S. (2015). Evaluation of antioxidant activity, phytochemicals and ESR Analysis of Lavandula Stoechas. Acta Phys Pol A, 128(2), 483-487. doi:10.12693/APhysPolA.128.B-483
  • Chai, T., Mohan, M., Ong, H., & Wong, F. (2014). Antioxidant, iron-chelating and anti-glucosidase activities of Typha domingensis Pers (Typhaceae). Tropical Journal of Pharmaceutical Research, 13(1), 67-72.
  • Clarke, G., Ting, K., Wiart, C., Fry, J. (2013). High Correlation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) Radical Scavenging, Ferric Reducing Activity Potential and Total Phenolics Content Indicates Redundancy in Use of All Three Assays to Screen for Antioxidant Activity of Extracts of Plants from the M. Antioxidants, 2(1), 1-10. doi:10.3390/antiox2010001
  • Ellman, G. L., Courtney, K. D., Andres Jr, V., & Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical pharmacology, 7(2), 88-95.
  • Eruygur, N., Uçar, E. (2018). Cholinesterase, α-Glucosidase, α-Amylase, and Tyrosinase Inhibitory Effects and Antioxidant Activity of Veronica officinalis Extracts. Türkiye Tarımsal Araştırmalar Dergisi, 5(3), 253-259.. doi:10.19159/tutad.425732
  • Fréchard, A., Fabre, N., Hannedouche, S., & Fourasté, I. (2002). Glucosinolates from Cardaria draba. Fitoterapia, 73(2), 177-178.
  • Halimi M. GC-MS analysis of n-hexane extract from aerial parts of Cardaria draba and phytochemistry studies GC-MS analysis of n- hexane extract from aerial parts of Cardaria draba and phytochemistry studies. In: Second National Conference on Applied Research of Chemistry, Biology and Geology, At Iran,Tehran-Tehran University.; 2014. https://www.civilica.com/Paper-CBGCONF02-CBGCONF02_015.html.
  • Kaya, Y., Aksakal, O., Sunar, S., Erturk, F. A., Bozari, S., Agar, G., ... & Battal, P. (2015). Phytotoxical effect of Lepidium draba L. extracts on the germination and growth of monocot (Zea mays L.) and dicot (Amaranthus retroflexus L.) seeds. Toxicology and Industrial Health, 31(3), 247-254.
  • Kim, Y. M., Jeong, Y. K., Wang, M. H., Lee, W. Y., & Rhee, H. I. (2005). Inhibitory effect of pine extract on α-glucosidase activity and postprandial hyperglycemia. Nutrition, 21(6), 756-761.
  • Mahomoodally, M. F., Zengin, G., Aumeeruddy, M. Z., Sezgin, M., & Aktumsek, A. (2018). Phytochemical profile and antioxidant properties of two Brassicaceae species: Cardaria draba subsp. draba and Descurainia sophia. Biocatalysis and agricultural biotechnology, 16, 453-458.
  • Naser, E. H., Khthr, M. F. A., & Abed, S. A. (2019). Phytochemistry and therapeutic uses of Cardaria draba L.: a review. Plant Arch., 19(Suppl. 1), 118-125.
  • Namjoyan, F., Jahangiri, A., Azemi, M. E., & Mousavi, H. (2016). Inhibitory Effects of Urginea maritima (L.) Baker, Zhumeria majdae Rech. F. and Wendelbo and Physalis divaricata D. Don Ethanolic Extracts on Mushroom Tyrosinase. Pharmaceutical Sciences, 22(2), 81-86.
  • Neagu, E., Radu, G. L., Albu, C., & Paun, G. (2018). Antioxidant activity, acetylcholinesterase and tyrosinase inhibitory potential of Pulmonaria officinalis and Centarium umbellatum extracts. Saudi journal of biological sciences, 25(3), 578-585.
  • Ouissem, B. S., Sabrina, B., Lotfi, B., Khellaf, R., Chawki, B., Ibrahim, D., ... & Fadila, B. (2018). HPLC Analysis and Antioxidant Properties of Algerian Lepidium draba Ethyl acetate Extract. Journal of Biologically Active Products from Nature, 8(4), 265-271.
  • Özek, G. (2018). Chemical diversity and biological potential of Tanacetum praeteritum subsp. praeteritum essential oils. Journal of the Turkish Chemical Society Section A: Chemistry, 5(2), 493-510.
  • Roughani, A., Miri, S. M., Hassandokht, M. R., Moradi, P., & Abdossi, V. (2018). Morphological variation of some Lepidium draba and L. latifolium populations. Taiwania, 63(1), 41-48. doi:10.6165/tai.2018.63.41
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine, 26(9-10), 1231-1237.
  • Sarikurkcu, C., Targan, S., Ozer, M. S., & Tepe, B. (2017). Fatty acid composition, enzyme inhibitory, and antioxidant activities of the ethanol extracts of selected wild edible plants consumed as vegetables in the Aegean region of Turkey. International Journal of Food Properties, 20(3), 560-572. doi:10.1080/10942912.2016.1168837
  • Senatore, F., Rigano, D., Grassia, A., & Randazzo, A. (2003). 4-hydroxybenzyl glucosinolate from Cardaria draba (Cruciferae). Biochemical systematics and ecology, 10(31), 1205-1207.
  • Sharifi-Rad, J., Hoseini-Alfatemi, S. M., Sharifi-Rad, M., da Silva, J. A. T., Rokni, M., & Sharifi-Rad, M. (2015). Evaluation of biological activity and phenolic compounds of Cardaria draba (L.) extracts. J. Biol. Today's World, 4(9), 180-189.
  • Seebaluck-Sandoram, R., Lall, N., Fibrich, B., Blom van Staden, A., Saleem, H., & Mahomoodally, M. F. (2019). Biocatalysis and Agricultural Biotechnology Antimicrobial, antioxidant and cytotoxic evaluation of two underutilised food plants: Averrhoa bilimbi L.(Oxalidaceae) and Phyllanthus acidus L. Skeels (Phyllanthaceae). Biocatalysis and Agricultural Biotechnology, 18, 1-6. doi:10.1016/j.bcab.2019.01.036
  • Yildiztekin, F., Nadeem, S., Erol, E., Yildiztekin, M., Tuna, A. L., & Ozturk, M. (2016). Antioxidant, anticholinesterase and tyrosinase inhibition activities, and fatty acids of Crocus mathewii–A forgotten endemic angiosperm of Turkey. Pharmaceutical biology, 54(9), 1557-1563. doi:10.3109/13880209.2015.1107746
  • Yang, Y., Gu, L., Xiao, Y., Liu, Q., Hu, H., Wang, Z., & Chen, K. (2015). Rapid identification of α-glucosidase inhibitors from Phlomis tuberosa by Sepbox chromatography and thin-layer chromatography bioautography. PLoS One, 10(2), e0116922. doi:10.1371/journal.pone.0116922

Phenolic Composition, In-vitro Antioxidant and Enzyme Inhibition Activities of Cardaria draba Different Parts

Yıl 2022, Sayı: 35, 424 - 431, 07.05.2022

Nuraniye Eruygur Fatma Ayaz Yavuz Bağcı Ebru Güler Esra Maltaş Çağıl

https://doi.org/10.31590/ejosat.1062109
Cited By: 1

Öz

The plant Cardaria draba (L.) Desv. subsp. draba (Brassicaceae), is traditionally used for various ailments in different cultures. We investigated different solvent extracts such as methanol, ethanol, and water extracts obtained from flowers, leaves, stems, and roots of C. draba for their chemical composition, as well as antioxidant and enzyme inhibition activities of acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-glucosidase, α-amylase, and tyrosinase). RP-HPLC technique was used to determine the phenolic profile of each extracts. The antioxidant activities of the extracts were determined by using DPPH and ABTS method, total phenol and flavonoid content analysis, and iron chelating assay. Enzyme inhibitory potential was evaluated by 96-microplate method. According to the results, aqueous extract of flower demonstrated the highest total phenolic content (64.32 µgGAE/ mg), while leaf ethanol extract showed the highest total flavonoid content (141.47 µgQE/ mg) among all extracts. The methanol, and water extracts of flowers were the most effective DPPH scavenger while aqueous extract of flower was the most active on ABTS scavenging. It is shown that the extracts possess promising activity against α-glucosidase than α-amylase enzyme. The methanolic extract of flower showed anti-acetylcholinesterase activity, and the ethanolic extract of the stem exhibited the best anti-butyrylcholinesterase activity among the 12 extracts. As for the anti-tyrosinase activity, higher kojic acid equivalent values were found for the aqueous extracts of roots and leaves. In conclusion, the data obtained from this study may serve as the basis for the bioassay-guided isolation of active compounds and the development of novel drugs from Cardaria draba.

Anahtar Kelimeler

Cardaria draba Antioxidant activity Enzyme inhibition HPLC

Kaynakça

  • Askin, H., Yildiz, M., Ayar, A. (2017). Effects of thymol and carvacrol on acetylcholinesterase from Drosophila melanogaster. Acta Phys Pol A, 132(3), 720-722.
  • Ainsworth, E. A., & Gillespie, K. M. (2007). Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. Nature protocols, 2(4), 875-877.
  • Bag, G.C., Grihanjali Devi, P., Bhaigyaba, T. (2015). Assessment of total flavonoid content and antioxidant activity of methanolic rhizome extract of three Hedychium species of Manipur valley. Int J Pharm Sci Rev Res., 30(1),154-159.
  • Boutemak, K., Safta, B., & Ayachi, N. (2015). Study of the Anti-Inflammatory Activity of Flavonic Extract of Globularia alypum L. Acta Physica Polonica A, 128(2B), B-239..
  • Benahmed-Bouhafsoun, A., Djebbar, H., & Kaid-Harche, M. (2015). Determination of Polyphenolic Compounds of Washingtonia robusta H. Wendl Extracts. Acta Physica Polonica A, 128(2B), B-465.
  • Bicha, S., Benmekhebi, L., Boubekri, N., Khellaf, R., Brouard, I., Zama, D., ... & Benayache, F. (2016). Compositional study, antibacterial and antioxidant potential of Lepidium draba L.(Brascicaceae). Res J Pharm Biol Chem Sci., 7(2), 283-287.
  • Ceylan, Y., Usta, K., Usta, A., Maltas, E., Yildiz, S. (2015). Evaluation of antioxidant activity, phytochemicals and ESR Analysis of Lavandula Stoechas. Acta Phys Pol A, 128(2), 483-487. doi:10.12693/APhysPolA.128.B-483
  • Chai, T., Mohan, M., Ong, H., & Wong, F. (2014). Antioxidant, iron-chelating and anti-glucosidase activities of Typha domingensis Pers (Typhaceae). Tropical Journal of Pharmaceutical Research, 13(1), 67-72.
  • Clarke, G., Ting, K., Wiart, C., Fry, J. (2013). High Correlation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) Radical Scavenging, Ferric Reducing Activity Potential and Total Phenolics Content Indicates Redundancy in Use of All Three Assays to Screen for Antioxidant Activity of Extracts of Plants from the M. Antioxidants, 2(1), 1-10. doi:10.3390/antiox2010001
  • Ellman, G. L., Courtney, K. D., Andres Jr, V., & Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical pharmacology, 7(2), 88-95.
  • Eruygur, N., Uçar, E. (2018). Cholinesterase, α-Glucosidase, α-Amylase, and Tyrosinase Inhibitory Effects and Antioxidant Activity of Veronica officinalis Extracts. Türkiye Tarımsal Araştırmalar Dergisi, 5(3), 253-259.. doi:10.19159/tutad.425732
  • Fréchard, A., Fabre, N., Hannedouche, S., & Fourasté, I. (2002). Glucosinolates from Cardaria draba. Fitoterapia, 73(2), 177-178.
  • Halimi M. GC-MS analysis of n-hexane extract from aerial parts of Cardaria draba and phytochemistry studies GC-MS analysis of n- hexane extract from aerial parts of Cardaria draba and phytochemistry studies. In: Second National Conference on Applied Research of Chemistry, Biology and Geology, At Iran,Tehran-Tehran University.; 2014. https://www.civilica.com/Paper-CBGCONF02-CBGCONF02_015.html.
  • Kaya, Y., Aksakal, O., Sunar, S., Erturk, F. A., Bozari, S., Agar, G., ... & Battal, P. (2015). Phytotoxical effect of Lepidium draba L. extracts on the germination and growth of monocot (Zea mays L.) and dicot (Amaranthus retroflexus L.) seeds. Toxicology and Industrial Health, 31(3), 247-254.
  • Kim, Y. M., Jeong, Y. K., Wang, M. H., Lee, W. Y., & Rhee, H. I. (2005). Inhibitory effect of pine extract on α-glucosidase activity and postprandial hyperglycemia. Nutrition, 21(6), 756-761.
  • Mahomoodally, M. F., Zengin, G., Aumeeruddy, M. Z., Sezgin, M., & Aktumsek, A. (2018). Phytochemical profile and antioxidant properties of two Brassicaceae species: Cardaria draba subsp. draba and Descurainia sophia. Biocatalysis and agricultural biotechnology, 16, 453-458.
  • Naser, E. H., Khthr, M. F. A., & Abed, S. A. (2019). Phytochemistry and therapeutic uses of Cardaria draba L.: a review. Plant Arch., 19(Suppl. 1), 118-125.
  • Namjoyan, F., Jahangiri, A., Azemi, M. E., & Mousavi, H. (2016). Inhibitory Effects of Urginea maritima (L.) Baker, Zhumeria majdae Rech. F. and Wendelbo and Physalis divaricata D. Don Ethanolic Extracts on Mushroom Tyrosinase. Pharmaceutical Sciences, 22(2), 81-86.
  • Neagu, E., Radu, G. L., Albu, C., & Paun, G. (2018). Antioxidant activity, acetylcholinesterase and tyrosinase inhibitory potential of Pulmonaria officinalis and Centarium umbellatum extracts. Saudi journal of biological sciences, 25(3), 578-585.
  • Ouissem, B. S., Sabrina, B., Lotfi, B., Khellaf, R., Chawki, B., Ibrahim, D., ... & Fadila, B. (2018). HPLC Analysis and Antioxidant Properties of Algerian Lepidium draba Ethyl acetate Extract. Journal of Biologically Active Products from Nature, 8(4), 265-271.
  • Özek, G. (2018). Chemical diversity and biological potential of Tanacetum praeteritum subsp. praeteritum essential oils. Journal of the Turkish Chemical Society Section A: Chemistry, 5(2), 493-510.
  • Roughani, A., Miri, S. M., Hassandokht, M. R., Moradi, P., & Abdossi, V. (2018). Morphological variation of some Lepidium draba and L. latifolium populations. Taiwania, 63(1), 41-48. doi:10.6165/tai.2018.63.41
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine, 26(9-10), 1231-1237.
  • Sarikurkcu, C., Targan, S., Ozer, M. S., & Tepe, B. (2017). Fatty acid composition, enzyme inhibitory, and antioxidant activities of the ethanol extracts of selected wild edible plants consumed as vegetables in the Aegean region of Turkey. International Journal of Food Properties, 20(3), 560-572. doi:10.1080/10942912.2016.1168837
  • Senatore, F., Rigano, D., Grassia, A., & Randazzo, A. (2003). 4-hydroxybenzyl glucosinolate from Cardaria draba (Cruciferae). Biochemical systematics and ecology, 10(31), 1205-1207.
  • Sharifi-Rad, J., Hoseini-Alfatemi, S. M., Sharifi-Rad, M., da Silva, J. A. T., Rokni, M., & Sharifi-Rad, M. (2015). Evaluation of biological activity and phenolic compounds of Cardaria draba (L.) extracts. J. Biol. Today's World, 4(9), 180-189.
  • Seebaluck-Sandoram, R., Lall, N., Fibrich, B., Blom van Staden, A., Saleem, H., & Mahomoodally, M. F. (2019). Biocatalysis and Agricultural Biotechnology Antimicrobial, antioxidant and cytotoxic evaluation of two underutilised food plants: Averrhoa bilimbi L.(Oxalidaceae) and Phyllanthus acidus L. Skeels (Phyllanthaceae). Biocatalysis and Agricultural Biotechnology, 18, 1-6. doi:10.1016/j.bcab.2019.01.036
  • Yildiztekin, F., Nadeem, S., Erol, E., Yildiztekin, M., Tuna, A. L., & Ozturk, M. (2016). Antioxidant, anticholinesterase and tyrosinase inhibition activities, and fatty acids of Crocus mathewii–A forgotten endemic angiosperm of Turkey. Pharmaceutical biology, 54(9), 1557-1563. doi:10.3109/13880209.2015.1107746
  • Yang, Y., Gu, L., Xiao, Y., Liu, Q., Hu, H., Wang, Z., & Chen, K. (2015). Rapid identification of α-glucosidase inhibitors from Phlomis tuberosa by Sepbox chromatography and thin-layer chromatography bioautography. PLoS One, 10(2), e0116922. doi:10.1371/journal.pone.0116922
Toplam 29 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Mühendislik
Bölüm Makaleler
Yazarlar

Nuraniye Eruygur 0000-0002-4674-7009

Fatma Ayaz 0000-0003-3994-6576

Yavuz Bağcı 0000-0002-2343-3672

Ebru Güler 0000-0002-1230-2810

Esra Maltaş Çağıl 0000-0001-7574-6016

Yayımlanma Tarihi 7 Mayıs 2022
Yayımlandığı Sayı Yıl 2022 Sayı: 35

Kaynak Göster

APA Eruygur, N., Ayaz, F., Bağcı, Y., Güler, E., vd. (2022). Phenolic Composition, In-vitro Antioxidant and Enzyme Inhibition Activities of Cardaria draba Different Parts. Avrupa Bilim Ve Teknoloji Dergisi(35), 424-431. https://doi.org/10.31590/ejosat.1062109

Cited By

Antioxidant Properties, Total Phenolic Content and LC-MS/MS Analysis of Mentha Pulegium, Lepidium Draba and Centaurea Solstitialis
Journal of the Institute of Science and Technology
https://doi.org/10.21597/jist.1177585
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