Araştırma Makalesi
Characterization of Rubidium-Based Nanoparticles by Green Synthesis and Their Effect on Colorectal Cancer Cells
Öz
Objective: Colorectal cancers pose a major threat along with increasing morbidity and mortality to human health worldwide. Therefore, it is crucial to develop effective and safe methods for tumor therapy. In recent years, nanoparticles have emerged as successful candidates for drug delivery into tumor tissue. The particle size of nanoparticles (NPs) is of great importance for passive tumor targeting. Therefore, in this study, we aimed to synthesize and characterize rubidium-based nanoparticles (RbNPs) from the moss Abietinella abietina (AA) and determine their anticancer effects on colorectal carcinoma cell line (HCT116).
Materials and Methods: A field emission scanning electron microscope (FESEM), dynamic light scattering (DLS), energy dispersive X-ray analysis (EDX), UV/VIS and fourier transform infrared (FTIR) spectrophotometers were used to characterize the RbNPs. To study the cytotoxicity, a sulforhodamine B (SRB) assay was performed in colorectal carcinoma cell cultures.
Results: As a result, RbNPs- AA developed with an average particle size of about 70 nm. RbNPs- AA proved to be cytotoxic at lower doses than free AA, as it decreased cell viability at half the amount of free AA (14.25 µg/mL).
Conclusion: The availability of RbNPs, particularly for the treatment of colorectal cancer, is evidenced by the fact that all the data collected are highly relevant.
Anahtar Kelimeler
Teşekkür
The Abietinella abietina moss specimens used in the study were obtained from the personal herbarium of Kerem Canlı. For this, we express our gratitude to him.
Kaynakça
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- 2. Saraiva MR, Rosa I, Claro I. Early-onset colorectal cancer: A review of current knowledge. World J Gastroenterol 2023; 29(8): 1289-303. google scholar
- 3. Bonelli J, Velasco-de Andres M, Isidro N, Bayo C, Chumillas S, Carrillo-Serradell L, et al. Novel tumor-targeted self-nanostructured and compartmentalized water-in-oil-in-water polyurethane-polyurea nanocapsules for cancer theragnosis. Pharmaceutics 2022; 15(1): 58. google scholar
- 4. Nath D, Banerjee P. Green nanotechnology - a new hope for medical biology. Environ Toxicol Pharmacol 2013; 36(3): 997-1014. google scholar
- 5. Shahverdi AR, Fakhimi A, Shahverdi HR, Minaian S. Synthesis and effect of silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli. Nanomedicine 2007; 3(2): 168-71. google scholar
- 6. Makarov VV, Love AJ, Sinitsyna OV, Makarova SS, Yaminsky IV, Taliansky ME, et al. “Green” Nanotechnologies: Synthesis of metal nanoparticles using plants. Acta Naturae 2014; 6: 35. google scholar
- 7. Ritter SK. EPA Data suggest green success. Chemical & Engineering News. 2015; 93: 32-3. google scholar
- 8. Nasrollahzadeh M, Issaabadi Z, Sajadi SM. Green synthesis of a Cu/MgO nanocomposite by Cassytha filiformis L. extract and investigation of its catalytic activity in the reduction of methylene blue, congo red and nitro compounds in aqueous media. RSC Adv 2018; 8: 3723-35. google scholar
- 9. Clayton WA, Albert NW, Thrimawithana AH, McGhie TK, Deroles SC, Schwinn KE. et al. UVR8-mediated induction of flavonoid biosynthesis for UVB tolerance is conserved between the liverwort Marchantia polymorpha and flowering plants. Plant J 2018 ; 96: 503-17. google scholar
- 10. Bandyopadhyay A, Dey A. The ethno-medicinal and pharmaceutical attributes of Bryophytes: A review. Phytomed Plus 2022; 2: 100255. google scholar
- 11. “Abietinella abietina var. abietina/histricosa”. Royal Botanic Garden Edinburgh. Retrieved 2023-05-18. Available from: URL: https://www. britishbryologicalsociety.org.uk/wp-content/uploads/2020/12/ Abietinella-abietina-var.-abietina-histricosa.pdf. google scholar
- 12. Spjut RW, Suffness M, Cragg GM, Norris DH. Mosses, hornworts and liverworts screened for antitumor activity. Economic Botany 1986; 40: 310-38. google scholar
- 13. Özerkan D, Erol A, Altuner EM, Canlı K, Kuruca DS. Some bryophytes trigger cytotoxicity of stem cell-like population in 5-fluorouracil resistant colon cancer cells. Nutr Cancer 2022; 74(3): 1012-22. google scholar
- 14. Orellana EA, Kasinski AL. Sulforhodamine B (SRB) assay in cell culture to investigate cell proliferation. Bio Protoc 2016; 6: 21. google scholar
- 15. Andrezalova L, Orszaghova Z. Covalent and noncovalent interactions of coordination compounds with DNA: An overview. J Inorg Biochem 2021; 225: 111624. google scholar
- 16. Souri M, Soltani M, Moradi Kashkooli F, Shahvandi MK. Engineered strategies to enhance tumor penetration of drug-loaded nanoparticles. J Control Release 2022; 3(41): 227-46. google scholar
- 17. Yadav KS, Dalal DC. The heterogeneous multiscale method to study particle size and partitioning effects in drug delivery. Comput Math Appl 2021; 92: 134-48. google scholar
- 18. Ikeda-Imafuku M, Wang LLW, Rodrigues D, Shaha S, Zhao Z, Mitragotri S. Strategies to improve the EPR effect: A mechanistic perspective and clinical translation. J Control Release 2022; 345: 512-36. google scholar
- 19. Fang J, Islam W, Maeda H. Exploiting the dynamics of the EPR effect and strategies to improve the therapeutic effects of nanomedicines by using EPR effect enhancers. Adv Drug Deliv Rev 2020; 157: 142-60. google scholar
- 20. Liu Y, Zhou J, Li Q, Li L, Jia Y, Geng F. et al. Tumor microenvironment remodeling-based penetration strategies to amplify nanodrug accessibility to tumor parenchyma. Adv Drug Deliv Rev 2021; 172: 80-103. google scholar
- 21. Zi Y, Yang K, He J Wu Z, Liu J, Zhang W. Strategies to enhance drug delivery to solid tumors by harnessing the EPR effects and alternative targeting mechanisms. Adv Drug Deliv Rev 2022; 114449. google scholar
- 22. Xu J, Song M, Fang Z, Zheng L, Huang X, Liu K. Applications and challenges of ultra-small particle size nanoparticles in tumor therapy. J Control Release 2023; 353: 699-712. google scholar
- 23. Jiao Y, Meng F, Zhu G, Ran LZ, Jiang YF, Zhang Q. Synthesis of a novel p-hydroxycinnamic amide with anticancer capability and its interaction with human serum albumin. Exp Ther Med 2019; 17: 1321-29. google scholar
- 24. Titus D, James Jebaseelan Samuel E, Roopan SM. Nanoparticle characterization techniques. Shukla AK, Iravani S, editors. Micro and nano technologies, green synthesis, characterization and applications of nanoparticles. Elsevier; 2019. p. 303-19. google scholar
- 25. Fissan H, Ristig S, Kaminski H, Asbach C, Epple M. Comparison of different characterization methods for nanoparticle dispersions before and after aerosolization. Analytical Methods 2014; 6: 7324-34. google scholar
- 26. Rades S, Hodoroaba VD, Salge T, Wirth T, Lobera MP, Labrador RH. High-resolution imaging with SEM/T-SEM, EDX and SAM as a combined methodical approach for morphological and elemental analyses of single engineered nanoparticles. RSC Adv 2014; 4: 49577-87. google scholar
- 27. Suba A, Selvarajan P, Jebaraj Devadasan J. Rubidium chloride doped magnesium oxide nanomaterial by using green synthesis and its characterization. Chem Phys Lett 2022; 793: 139463. google scholar
- 28. Ouyang S, Zheng K, Huang Q, Liu Y, Boccaccini AR. Synthesis and characterization of rubidium-containing bioactive glass nanoparticles. Mater Lett 2020; 273: 127920. google scholar
- 29. Khorshid FA, Raouf GA, El-Hamidy SM, Al-amri GS, Alotaibi NA. PMF, cesium & rubidium nanoparticles induce apoptosis in A549 cells. Life Sci J 2011; 8(3): 534-42. google scholar
Yıl 2023,
Cilt: 13 Sayı: 2, 97 - 102, 18.09.2023
Öz
Kaynakça
- 1. Xi Y, Xu P. Global colorectal cancer burden in 2020 and projections to 2040. Transl Oncol 2021; 14(10): 101174. google scholar
- 2. Saraiva MR, Rosa I, Claro I. Early-onset colorectal cancer: A review of current knowledge. World J Gastroenterol 2023; 29(8): 1289-303. google scholar
- 3. Bonelli J, Velasco-de Andres M, Isidro N, Bayo C, Chumillas S, Carrillo-Serradell L, et al. Novel tumor-targeted self-nanostructured and compartmentalized water-in-oil-in-water polyurethane-polyurea nanocapsules for cancer theragnosis. Pharmaceutics 2022; 15(1): 58. google scholar
- 4. Nath D, Banerjee P. Green nanotechnology - a new hope for medical biology. Environ Toxicol Pharmacol 2013; 36(3): 997-1014. google scholar
- 5. Shahverdi AR, Fakhimi A, Shahverdi HR, Minaian S. Synthesis and effect of silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli. Nanomedicine 2007; 3(2): 168-71. google scholar
- 6. Makarov VV, Love AJ, Sinitsyna OV, Makarova SS, Yaminsky IV, Taliansky ME, et al. “Green” Nanotechnologies: Synthesis of metal nanoparticles using plants. Acta Naturae 2014; 6: 35. google scholar
- 7. Ritter SK. EPA Data suggest green success. Chemical & Engineering News. 2015; 93: 32-3. google scholar
- 8. Nasrollahzadeh M, Issaabadi Z, Sajadi SM. Green synthesis of a Cu/MgO nanocomposite by Cassytha filiformis L. extract and investigation of its catalytic activity in the reduction of methylene blue, congo red and nitro compounds in aqueous media. RSC Adv 2018; 8: 3723-35. google scholar
- 9. Clayton WA, Albert NW, Thrimawithana AH, McGhie TK, Deroles SC, Schwinn KE. et al. UVR8-mediated induction of flavonoid biosynthesis for UVB tolerance is conserved between the liverwort Marchantia polymorpha and flowering plants. Plant J 2018 ; 96: 503-17. google scholar
- 10. Bandyopadhyay A, Dey A. The ethno-medicinal and pharmaceutical attributes of Bryophytes: A review. Phytomed Plus 2022; 2: 100255. google scholar
- 11. “Abietinella abietina var. abietina/histricosa”. Royal Botanic Garden Edinburgh. Retrieved 2023-05-18. Available from: URL: https://www. britishbryologicalsociety.org.uk/wp-content/uploads/2020/12/ Abietinella-abietina-var.-abietina-histricosa.pdf. google scholar
- 12. Spjut RW, Suffness M, Cragg GM, Norris DH. Mosses, hornworts and liverworts screened for antitumor activity. Economic Botany 1986; 40: 310-38. google scholar
- 13. Özerkan D, Erol A, Altuner EM, Canlı K, Kuruca DS. Some bryophytes trigger cytotoxicity of stem cell-like population in 5-fluorouracil resistant colon cancer cells. Nutr Cancer 2022; 74(3): 1012-22. google scholar
- 14. Orellana EA, Kasinski AL. Sulforhodamine B (SRB) assay in cell culture to investigate cell proliferation. Bio Protoc 2016; 6: 21. google scholar
- 15. Andrezalova L, Orszaghova Z. Covalent and noncovalent interactions of coordination compounds with DNA: An overview. J Inorg Biochem 2021; 225: 111624. google scholar
- 16. Souri M, Soltani M, Moradi Kashkooli F, Shahvandi MK. Engineered strategies to enhance tumor penetration of drug-loaded nanoparticles. J Control Release 2022; 3(41): 227-46. google scholar
- 17. Yadav KS, Dalal DC. The heterogeneous multiscale method to study particle size and partitioning effects in drug delivery. Comput Math Appl 2021; 92: 134-48. google scholar
- 18. Ikeda-Imafuku M, Wang LLW, Rodrigues D, Shaha S, Zhao Z, Mitragotri S. Strategies to improve the EPR effect: A mechanistic perspective and clinical translation. J Control Release 2022; 345: 512-36. google scholar
- 19. Fang J, Islam W, Maeda H. Exploiting the dynamics of the EPR effect and strategies to improve the therapeutic effects of nanomedicines by using EPR effect enhancers. Adv Drug Deliv Rev 2020; 157: 142-60. google scholar
- 20. Liu Y, Zhou J, Li Q, Li L, Jia Y, Geng F. et al. Tumor microenvironment remodeling-based penetration strategies to amplify nanodrug accessibility to tumor parenchyma. Adv Drug Deliv Rev 2021; 172: 80-103. google scholar
- 21. Zi Y, Yang K, He J Wu Z, Liu J, Zhang W. Strategies to enhance drug delivery to solid tumors by harnessing the EPR effects and alternative targeting mechanisms. Adv Drug Deliv Rev 2022; 114449. google scholar
- 22. Xu J, Song M, Fang Z, Zheng L, Huang X, Liu K. Applications and challenges of ultra-small particle size nanoparticles in tumor therapy. J Control Release 2023; 353: 699-712. google scholar
- 23. Jiao Y, Meng F, Zhu G, Ran LZ, Jiang YF, Zhang Q. Synthesis of a novel p-hydroxycinnamic amide with anticancer capability and its interaction with human serum albumin. Exp Ther Med 2019; 17: 1321-29. google scholar
- 24. Titus D, James Jebaseelan Samuel E, Roopan SM. Nanoparticle characterization techniques. Shukla AK, Iravani S, editors. Micro and nano technologies, green synthesis, characterization and applications of nanoparticles. Elsevier; 2019. p. 303-19. google scholar
- 25. Fissan H, Ristig S, Kaminski H, Asbach C, Epple M. Comparison of different characterization methods for nanoparticle dispersions before and after aerosolization. Analytical Methods 2014; 6: 7324-34. google scholar
- 26. Rades S, Hodoroaba VD, Salge T, Wirth T, Lobera MP, Labrador RH. High-resolution imaging with SEM/T-SEM, EDX and SAM as a combined methodical approach for morphological and elemental analyses of single engineered nanoparticles. RSC Adv 2014; 4: 49577-87. google scholar
- 27. Suba A, Selvarajan P, Jebaraj Devadasan J. Rubidium chloride doped magnesium oxide nanomaterial by using green synthesis and its characterization. Chem Phys Lett 2022; 793: 139463. google scholar
- 28. Ouyang S, Zheng K, Huang Q, Liu Y, Boccaccini AR. Synthesis and characterization of rubidium-containing bioactive glass nanoparticles. Mater Lett 2020; 273: 127920. google scholar
- 29. Khorshid FA, Raouf GA, El-Hamidy SM, Al-amri GS, Alotaibi NA. PMF, cesium & rubidium nanoparticles induce apoptosis in A549 cells. Life Sci J 2011; 8(3): 534-42. google scholar
Toplam 29 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Klinik Tıp Bilimleri (Diğer) |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 18 Eylül 2023 |
| Gönderilme Tarihi | 19 Nisan 2023 |
| Yayımlandığı Sayı | Yıl 2023 Cilt: 13 Sayı: 2 |
