Araştırma Makalesi
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Identification of phenolic content, antibacterial and antioxidant activities of Lonicera caucasica PALLAS subsp. orientalis leaves

Yıl 2023, Cilt: 4 Sayı: 2, 85 - 91, 30.08.2023

Mehmet Emin Şeker

https://doi.org/10.51753/flsrt.1286886

Öz

Phenolic compounds, which are a prevalent type of secondary metabolite, are responsible for the antioxidant and antimicrobial properties found in the extracts. To contribute new knowledge to scientific literature, it is essential to study the phenolic content and bioactivity of plant parts that have not been previously studied or are unknown. Lonicera caucasica PALLAS subsp. orientalis is an endemic species that grows in a few cities in Türkiye. The study involved extracting L. caucasica leaves using methanol. The resulting fraction was then analyzed using Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) to determine the phenolic content of the extract. The quantities of 22 out of the 67 phenolic compounds that were identified were determined. Among these, the top three in terms of quantity are chlorogenic acid, with a concentration of 2.223 ± 0.065 mg g-1, followed by quinic acid at 2.163 ± 0.057 mg g-1, and syringic acid at 1.192 ± 0.036 mg g-1. The antioxidant activities of the extracts were determined using the ABTS•+, DPPH (SC50, mg/mL) and FRAP (μM, TEAC) methods. The values were calculated to be 0.0135 ± 0.0001, 0.0136 ± 0.0001, and 2368.88 ± 2.94, respectively. Furthermore, the extracted substance was subjected to tests against three gram-negative bacteria (Pseudomonas aeruginosa, Klebsiella aerogenes, and Escherichia coli) and three gram-positive bacteria (Enterococcus faecalis, Bacillus megaterium, and Streptococcus pyogenes). The outcomes of the tests were documented. The highest level of antibacterial activity was found to be against P. aeruginosa. Based on the findings, it has been determined that the leaves of L. caucasica contain a considerable amount of phenolic content, exhibit exceptional antioxidant activity, and display a strong antibacterial effect.

Anahtar Kelimeler

Antimicrobial activity antioxidant activity phenolic content total phenolic content

Destekleyen Kurum

Giresun University

Proje Numarası

FEN-BAP-A-240222-44

Teşekkür

This study was supported in 2022 by Giresun University's Scientific Research Projects Commission under the project number FEN-BAP-A-240222-44. I would like to thank Giresun University Scientific Research Projects Commission for its support.

Kaynakça

  • Asaadi, A. M. (2021). Study of medicinal herbs used by Kurmanjis Kurdish of Bojnourd in North Khorasan province of Iran. Journal of Ethno-Pharmaceutical Products, 2(2), 21-34.
  • Benzie, I. F., & Strain, J. J. (1999). [2] Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. In: Packer L. (ed) Methods in Enzymology (Vol. 299, pp. 15-27). Academic Press.
  • Byun, M. W., Jo, C., Jeon, T. W., & Hong, C. H. (2004a). Effects of gamma irradiation on color characteristics and biological activities of extracts of Lonicera japonica (Japanese honeysuckle) with methanol and acetone. LWT-Food Science and Technology, 37(1), 29-33.
  • Byun, M. W., Jo, C., Lee, J. W., Jo, S. K., & Kim, K. S. (2004b). Application of radiation technology to develop green tea leaf as a natural resource for the cosmetic industry. Radiation Physics and Chemistry, 71(1-2), 487-489.
  • Cao, W., Chen, J., Li, L., Ren, G., Duan, X., Zhou, Q., ... & Liu, X. (2022). Cookies fortified with Lonicera japonica Thunb. extracts: Impact on phenolic acid content, antioxidant activity and physical properties. Molecules, 27(15), 5033.
  • Cipollini, D., Stevenson, R., Enright, S., Eyles, A., & Bonello, P. (2008). Phenolic metabolites in leaves of the invasive shrub, Lonicera maackii, and their potential phytotoxic and anti-herbivore effects. Journal of Chemical Ecology, 34, 144-152.
  • Cuendet, M., Hostettmann, K., Potterat, O., & Dyatmiko, W. (1997). Iridoid glucosides with free radical scavenging properties from Fagraea blumei. Helvetica Chimica Acta, 80(4), 1144-1152.
  • Dogan, A. (2014). Pertek (Tunceli) yöresinde etnobotanik araştırmalar, Doctoral dissertation, (pp. 1-459). Marmara Universitesi, Türkiye.
  • Dogan, A., & Tuzlaci, E. (2015). Tunceli’nin bazı yöresel bitki adları. Avrasya Terim Dergisi, 3(2), 23-33.
  • Dung, N. T., Bajpai, V. K., Rahman, A., Yoon, J. I., & Kang, S. C. (2011). Phenolic contents, antioxidant and tyrosinase inhibitory activities of Lonicera japonica Thumb. Journal of Food Biochemistry, 35(1), 148-160.
  • Farboodniay Jahromi, M. A., Emami, A., Nazeri, R., & Pirbonyeh, N. (2020). Antibacterial and antioxidant activity of extract and fractions of Lonicera nummularifolia Jaub & Spach. Trends in Pharmaceutical Sciences, 6(2), 131-142.
  • Ferrazzano, G. F., Amato, I., Ingenito, A., De Natale, A., & Pollio, A. (2009). Anti-cariogenic effects of polyphenols from plant stimulant beverages (cocoa, coffee, tea). Fitoterapia, 80(5), 255-262.
  • Iskender, E., Zeynalov, Y., Ozaslan, M., Incik, F., & Yayla, F. (2006). Investigation and introduction of some rare and threatened plants from Turkey. Biotechnology & Biotechnological Equipment, 20(3), 60-68.
  • Karacelik, A. A., Kucuk, M., Iskefiyeli, Z., Aydemir, S., De Smet, S., Miserez, B., & Sandra, P. (2015). Antioxidant components of Viburnum opulus L. determined by on-line HPLC–UV–ABTS radical scavenging and LC–UV–ESI-MS methods. Food Chemistry, 175, 106-114.
  • Karacelik, A. A., & Sahin, H. (2021a). Determination of chemical compositions, antioxidant and enzyme inhibitory activities of naturally growing Chenopodium album subsp. iranicum Aellen. Journal of the Institute of Science and Technology, 11(3), 2091-2101.
  • Karacelik, A. A., Kucuk, M., Efe, D., Cakir, V., & Biyiklioglu, Z. (2021b). Carbonic anhydrase inhibition potential and some bioactivities of the peripherally tetrasubstituted cobalt (II), titanium (IV), manganese (III) phthalocyanines. Letters in Drug Design & Discovery, 18(4), 365-371.
  • Mehrnia, M., Akaberi, M., Amiri, M. S., Nadaf, M., & Emami, S. A. (2021). Ethnopharmacological studies of medicinal plants in central Zagros, Lorestan Province, Iran. Journal of Ethnopharmacology, 280, 114080.
  • Palabas, S., Uzun, A., Terzioglu, S., & Ansin, R. (2005). Trabzon ilinin korunan doğal alanları ve bitkisel tür zenginliği. Korunan Doğal Alanlar Sempozyumu, Isparta, Türkiye. 63-65.
  • Petti, S., & Scully, C. (2009). Polyphenols, oral health and disease: A review. Journal of Dentistry, 37(6), 413-423.
  • Orsavová, J., Sytařová, I., Mlček, J., & Mišurcová, L. (2022). Phenolic compounds, vitamins C and E and antioxidant activity of edible honeysuckle berries (Lonicera caerulea L. var. kamtschatica Pojark) in relation to their origin. Antioxidants, 11(2), 433.
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237.
  • Sakinoglu-Oruc, F. Ç., & Oruc, S. H. (2017, May). Ethnobotonical aspects of some species in Düzce and its vicinity. I. International Congress on Medicinal and Aromatic Plants, Konya, Türkiye. 391-399.
  • Seo, O. N., Kim, G. S., Park, S., Lee, J. H., Kim, Y. H., Lee, W. S., ... & Shin, S. C. (2012). Determination of polyphenol components of Lonicera japonica Thunb. using liquid chromatography-tandem mass spectrometry: contribution to the overall antioxidant activity. Food Chemistry, 134(1), 572-577.
  • Slinkard, K., & Singleton, V. L. (1977). Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture, 28(1), 49-55.
  • Seker, M. E., Ay, E., Karacelik, A. A., Huseyinoglu, R., & Efe, D. (2021). First determination of some phenolic compounds and antimicrobial activities of Geranium ibericum subsp. jubatum: A plant endemic to Turkey. Turkish Journal of Chemistry, 45(1), 60-70.
  • Tomaszewski, D., Byalt, A., & Gawlak, M. (2019). Leaf and stem epicuticular wax structures in Lonicera species (Caprifoliaceae). Nordic Journal of Botany, 37(5).
  • Tunckol, B., Yasayacak, H., & Aksoy, N. (2020). Distribution patterns of rare Kitaibela vitifolia Willd in Turkey: Taxonomy, chorology, and conservation. Modern Phytomorphology, 14(1).
  • Turkucar, S. A., Karacelik, A. A., & Karakose, M. (2021). Phenolic compounds, essential oil composition, and antioxidant activity of Angelica pur-purascens (Avé-Lall.) Gill. Turkish Journal of Chemistry, 45(3), 956-966.
  • Yemis, G. P., Yemis, O., Drover, J. C., & Delaquis, P. (2022). Antibacterial activity of a polyphenol-rich haskap (Lonicera caerulea L.) extract and tannic acid against Cronobacter spp. Food Control, 140, 109120.

Yıl 2023, Cilt: 4 Sayı: 2, 85 - 91, 30.08.2023

Mehmet Emin Şeker

https://doi.org/10.51753/flsrt.1286886

Öz

Proje Numarası

FEN-BAP-A-240222-44

Kaynakça

  • Asaadi, A. M. (2021). Study of medicinal herbs used by Kurmanjis Kurdish of Bojnourd in North Khorasan province of Iran. Journal of Ethno-Pharmaceutical Products, 2(2), 21-34.
  • Benzie, I. F., & Strain, J. J. (1999). [2] Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. In: Packer L. (ed) Methods in Enzymology (Vol. 299, pp. 15-27). Academic Press.
  • Byun, M. W., Jo, C., Jeon, T. W., & Hong, C. H. (2004a). Effects of gamma irradiation on color characteristics and biological activities of extracts of Lonicera japonica (Japanese honeysuckle) with methanol and acetone. LWT-Food Science and Technology, 37(1), 29-33.
  • Byun, M. W., Jo, C., Lee, J. W., Jo, S. K., & Kim, K. S. (2004b). Application of radiation technology to develop green tea leaf as a natural resource for the cosmetic industry. Radiation Physics and Chemistry, 71(1-2), 487-489.
  • Cao, W., Chen, J., Li, L., Ren, G., Duan, X., Zhou, Q., ... & Liu, X. (2022). Cookies fortified with Lonicera japonica Thunb. extracts: Impact on phenolic acid content, antioxidant activity and physical properties. Molecules, 27(15), 5033.
  • Cipollini, D., Stevenson, R., Enright, S., Eyles, A., & Bonello, P. (2008). Phenolic metabolites in leaves of the invasive shrub, Lonicera maackii, and their potential phytotoxic and anti-herbivore effects. Journal of Chemical Ecology, 34, 144-152.
  • Cuendet, M., Hostettmann, K., Potterat, O., & Dyatmiko, W. (1997). Iridoid glucosides with free radical scavenging properties from Fagraea blumei. Helvetica Chimica Acta, 80(4), 1144-1152.
  • Dogan, A. (2014). Pertek (Tunceli) yöresinde etnobotanik araştırmalar, Doctoral dissertation, (pp. 1-459). Marmara Universitesi, Türkiye.
  • Dogan, A., & Tuzlaci, E. (2015). Tunceli’nin bazı yöresel bitki adları. Avrasya Terim Dergisi, 3(2), 23-33.
  • Dung, N. T., Bajpai, V. K., Rahman, A., Yoon, J. I., & Kang, S. C. (2011). Phenolic contents, antioxidant and tyrosinase inhibitory activities of Lonicera japonica Thumb. Journal of Food Biochemistry, 35(1), 148-160.
  • Farboodniay Jahromi, M. A., Emami, A., Nazeri, R., & Pirbonyeh, N. (2020). Antibacterial and antioxidant activity of extract and fractions of Lonicera nummularifolia Jaub & Spach. Trends in Pharmaceutical Sciences, 6(2), 131-142.
  • Ferrazzano, G. F., Amato, I., Ingenito, A., De Natale, A., & Pollio, A. (2009). Anti-cariogenic effects of polyphenols from plant stimulant beverages (cocoa, coffee, tea). Fitoterapia, 80(5), 255-262.
  • Iskender, E., Zeynalov, Y., Ozaslan, M., Incik, F., & Yayla, F. (2006). Investigation and introduction of some rare and threatened plants from Turkey. Biotechnology & Biotechnological Equipment, 20(3), 60-68.
  • Karacelik, A. A., Kucuk, M., Iskefiyeli, Z., Aydemir, S., De Smet, S., Miserez, B., & Sandra, P. (2015). Antioxidant components of Viburnum opulus L. determined by on-line HPLC–UV–ABTS radical scavenging and LC–UV–ESI-MS methods. Food Chemistry, 175, 106-114.
  • Karacelik, A. A., & Sahin, H. (2021a). Determination of chemical compositions, antioxidant and enzyme inhibitory activities of naturally growing Chenopodium album subsp. iranicum Aellen. Journal of the Institute of Science and Technology, 11(3), 2091-2101.
  • Karacelik, A. A., Kucuk, M., Efe, D., Cakir, V., & Biyiklioglu, Z. (2021b). Carbonic anhydrase inhibition potential and some bioactivities of the peripherally tetrasubstituted cobalt (II), titanium (IV), manganese (III) phthalocyanines. Letters in Drug Design & Discovery, 18(4), 365-371.
  • Mehrnia, M., Akaberi, M., Amiri, M. S., Nadaf, M., & Emami, S. A. (2021). Ethnopharmacological studies of medicinal plants in central Zagros, Lorestan Province, Iran. Journal of Ethnopharmacology, 280, 114080.
  • Palabas, S., Uzun, A., Terzioglu, S., & Ansin, R. (2005). Trabzon ilinin korunan doğal alanları ve bitkisel tür zenginliği. Korunan Doğal Alanlar Sempozyumu, Isparta, Türkiye. 63-65.
  • Petti, S., & Scully, C. (2009). Polyphenols, oral health and disease: A review. Journal of Dentistry, 37(6), 413-423.
  • Orsavová, J., Sytařová, I., Mlček, J., & Mišurcová, L. (2022). Phenolic compounds, vitamins C and E and antioxidant activity of edible honeysuckle berries (Lonicera caerulea L. var. kamtschatica Pojark) in relation to their origin. Antioxidants, 11(2), 433.
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237.
  • Sakinoglu-Oruc, F. Ç., & Oruc, S. H. (2017, May). Ethnobotonical aspects of some species in Düzce and its vicinity. I. International Congress on Medicinal and Aromatic Plants, Konya, Türkiye. 391-399.
  • Seo, O. N., Kim, G. S., Park, S., Lee, J. H., Kim, Y. H., Lee, W. S., ... & Shin, S. C. (2012). Determination of polyphenol components of Lonicera japonica Thunb. using liquid chromatography-tandem mass spectrometry: contribution to the overall antioxidant activity. Food Chemistry, 134(1), 572-577.
  • Slinkard, K., & Singleton, V. L. (1977). Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture, 28(1), 49-55.
  • Seker, M. E., Ay, E., Karacelik, A. A., Huseyinoglu, R., & Efe, D. (2021). First determination of some phenolic compounds and antimicrobial activities of Geranium ibericum subsp. jubatum: A plant endemic to Turkey. Turkish Journal of Chemistry, 45(1), 60-70.
  • Tomaszewski, D., Byalt, A., & Gawlak, M. (2019). Leaf and stem epicuticular wax structures in Lonicera species (Caprifoliaceae). Nordic Journal of Botany, 37(5).
  • Tunckol, B., Yasayacak, H., & Aksoy, N. (2020). Distribution patterns of rare Kitaibela vitifolia Willd in Turkey: Taxonomy, chorology, and conservation. Modern Phytomorphology, 14(1).
  • Turkucar, S. A., Karacelik, A. A., & Karakose, M. (2021). Phenolic compounds, essential oil composition, and antioxidant activity of Angelica pur-purascens (Avé-Lall.) Gill. Turkish Journal of Chemistry, 45(3), 956-966.
  • Yemis, G. P., Yemis, O., Drover, J. C., & Delaquis, P. (2022). Antibacterial activity of a polyphenol-rich haskap (Lonicera caerulea L.) extract and tannic acid against Cronobacter spp. Food Control, 140, 109120.
Toplam 29 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Bitki Biyokimyası
Bölüm Araştırma Makaleleri
Yazarlar

Mehmet Emin Şeker 0000-0003-4463-6898

Proje Numarası FEN-BAP-A-240222-44
Yayımlanma Tarihi 30 Ağustos 2023
Gönderilme Tarihi 24 Nisan 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 4 Sayı: 2

Kaynak Göster

APA Şeker, M. E. (2023). Identification of phenolic content, antibacterial and antioxidant activities of Lonicera caucasica PALLAS subsp. orientalis leaves. Frontiers in Life Sciences and Related Technologies, 4(2), 85-91. https://doi.org/10.51753/flsrt.1286886
 Kapak Resmi İndir

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Frontiers in Life Sciences and Related Technologies is licensed under a Creative Commons Attribution 4.0 International License.