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Karanlıkla Teşvik Edilen Yaprak Senesensi Sürecinde Gibberellik Asit ve 6-Benzilaminopürinin Bazı Biyokimyasal Parametreler Üzerine Etkisi

Yıl 2016, Cilt: 33 Sayı: 1, 17 - 24, 16.05.2016
https://doi.org/10.13002/jafag911

Öz

Bu çalışmada uzun süre karanlık ortamda bekletilerek senesens süreci uyarılan Tropaeolum majus L. (Latin çiçeği) yapraklarına, gibberellik asit (GA3) ve 6-benzilaminopürin (BAP) uygulamanın bazı senesens parametrelerine etkisi incelenmiştir. Bu amaçla 10-5 M BAP ve 10-5 M GA3 uygulanarak karanlık ortama bırakılan yaprakların klorofil, protein, nişasta ve hidrojen peroksit miktarları ile katalaz ve peroksidaz enzimlerinin aktiviteleri belirlenmiştir. Bu iki hormon yaprakların klorofil ve protein içeriğinde meydana gelen kaybı azaltırken nişasta içeriğindeki düşüşü engelleyememiştir. BAP ve GA3 katalaz aktivitesini kontrole göre anlamlı şekilde artırırken peroksidaz aktivitesini azaltmıştır. Hidrojen peroksit içeriği BAP ve GA3 uygulanan yapraklarda belirgin şekilde azalmıştır. Bu sonuçlar, BAP ve GA3 uygulamanın yaprak senesensinin biyokimyasal parametrelerini değiştirerek bu sürecin geciktirilmesinde etkili olduğunu göstermektedir.

Kaynakça

  • Angelini R, F. Manes and R. Federico (1990). Spatial and functional correlation between diamineoxidase and peroxidase activities and their dependence upon de-etiolation and wounding in chick-pea stems. Planta., 182: 89-96.
  • Arnon D.I (1949). Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol., 24: 1-15.
  • Bartoli C G, Simontacchi M, Guiamet J J, Montaldi E and Puntarulo S (1995). Antioxidant enzymes and lipid peroxidation during aging of Chrysanthemum morifolium RAM petals. Plant Science (104) 161-168.
  • Bazylkoa A, Parzonko A, Jeź W, Osińska E and Kiss A K (2014). Inhibition of ROS production, photoprotection, and total phenolic,flavonoids and ascorbic acid content of fresh herb juice and extractsfrom the leaves and flowers of Tropaeolum majus. Industrial Crops and Products (55) 19–24.
  • Bradford M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-251.
  • Costa M L, Cıvello P M, Chaves A R and Martınez GA (2005). Effect of ethephon and 6-benzylaminopurine on chlorophyll bleaching during Post-Harrest Senescence of Broccoli (Brassica oleracea L.) at 20 0C. Postharvest Biology and Technology, 35: 191-199.
  • Chang C J and Kao C H (1998). H2O2 metabolisim during senesecence of rice leaves:changes in enzyme activities in light and darkness. Plant Growth Regulation (25) 11-15.
  • Das K and Roychoudhury A (2014). Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants. Frontiers in Envıronmental Science. (2) 53. doi: 10.3389/fenvs.2014.00053.
  • Dennis VM and Winfield B A (1978). The determination of starch and cellulose in refuse and compost. Water Poll. Cont., 77: 529-531.
  • Dhindsa R S, Plumb-Dhindsa P L and Reid D M (1982). Leaf senescence and lipid peroxidation: effect of some phytohormones, and scavengers of free radicals and singlet oxygen. Physiol. Plant.(56) 453–457.
  • Downs C G, Somerfıeld S and Davey M C (1997). Cytokinin treatment delays senescence but not sucrose loss in harvested brocolli. Postharv. Biol. Tech (11) 93-100.
  • Duncan B.D (1955). Multiple range and multiple F-tests. Biometrics. P.1-42.
  • Gan S and Amasıno R.M (1995). Inhibition of Leaf Senescence by Autoregulated Production of Cytokinin, Science, 270, 1986–1988
  • Havir E A and Mchale N A (1987). Biochemical and developmental characterization of multiple forms of catalase in tobacco leaves. Plant Physiol., 84: 450-455.
  • Huang J, Han B, Xu S, Zhou M and Shen W (2011). Heme oxygenase-1 is involved in the cytokinin-induced alleviation of senescence in detached wheat leaves during dark incubation. Journal of Plant Physiology (168) 768–775.
  • Jiang W B, Lers A, Lomaniec E and Aharoni N (1999). Senescence-related serine protease in parsley. Phytochemistry (50) 377-382.
  • Jibran R, Hunter D A and Dijkwel P P (2013). Hormonal regulation of leaf senescence through integration of developmental and stress signals. Plant Mol Biol (82)547–561
  • Kanazawa S, Sano S, Koshıba T and Ushımaru T (2000). Changes in antioxidative enzymes in cucumber cotyledons during natural senescence: comparison with those during dark-ınduced senescence, Physiol. Plant. (109) 211-216.
  • Karuppanapandian T, Moon, J-C, Kim C, Manoharan K and Kim W (2011). Reactive oxygen species in plants: their generation, signal transduction, and scavenging mechanisms. AJCS 5, 709-725.
  • Khanna-Chopra R (2012). Leaf senescence and abiotic stresses share reactive oxygen species-mediated chloroplast degradation. Protoplasma (249) 469-481.
  • Li Li Z, Peng J, Wen X and Guo H (2012). Gene network analysis and functional studies of senescence-associated genes reveal novel regulators of Arabidopsis leaf senescence. Journal of Integrative Plant Biology 54 (8): 526–539.
  • Lim O P and Nam G H (2007). Aging and senescence of the leaf organ. Journal of Plant Biology 50 (3): 291-300.
  • Prochazkova D, Saıram R K, Srıvastava G C and Singh D V (2001). Oxidative stres and antioxidant activity as the basis of senescence in maize leaves. Plant Science (161) 765-771.
  • Racchi M L (2013). Antioxidant Defenses in Plants with Attention to Prunus and Citrus spp. Antioxidants (2) 340-369.
  • Ranwala A P and Miller W B (2000). Preventive mechanisms of gibberellin4+7 and light on low-temperature-induced leaf senescence in Lilium cv. Stargazer. Postharvest Biology and Technology, 19 : 85-92.
  • Rogers H J (2012). Is there an important role for reactive oxygen species and redox regulation during floral senescence? Plant, Cell and Environment. (35) 217–233.
  • Rosenvasser S, Mayak S and Friedman H (2006). Increase in reactive oxygen species ( ROS ) and in senescence-associated gene transcript ( SAG ) levels during dark-induced senescence of pelargonium cuttings, and the effect of gibberellic acid. Plant Science (170) 873–879.
  • Rosenwasser S, Belausov E, Riov J, Holdengreber V and Friedman H (2010). Gibberellic acid (GA3) Inhibits ros increase in chloroplasts during dark-ınduced senescence of pelargonium cuttings. J Plant Growth Regul (2010) 29: 375–384.
  • Sairam R K, Singh D V and Srıvastava G C (2003/4). Changes in activities of antioxidant enzymes in sunflower leaves of different ages. Biologia Plantarum,47(1):61-66.
  • Sarwat M, Naqvi A R, Ahmad P, Ashaf M and Akram N A (2013). Phytohormones and microRNAs as sensors and regulators of leaf senescence: Assigning macro roles to small molecules. Biotechnology Advances (31) 1153-1171.
  • Seema, Khokhar M and Mukherjee D (2011). Role of kinetin and a morphactin in leaf disc senescence of Raphanus sativus L. under low light. Physiol Mol Biol Plants 17(3):247–253.
  • Sharma P, Jha A B, Dubey R S and Pessarakli M (2012). Reactive oxygen species, oxidative damage, and antioxidative defensemechanism in plants under stressful conditions. Journal of Botany doi:10.1155/2012/217037.
  • Song Y, Yang C, Gao S, Zhang W, Li L andKuai B (2014). Age-triggered and dark-induced leaf senescence require the bHLH transcription factors PIF3, 4, and 5. Molecular Plant (7) 1776-1787.
  • Velikova V, I. Yordanov and A. Edrava (2000). Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Sci., 151: 59-66.
  • Xu F, Yang Z, Chen X, Jin P, Wang X and Zheng Y (2012). 6-Benzylaminopurine delays senescence and enhances health-promoting compounds of harvested Broccoli. J. Agric. Food Chem. (60) 234−240.
  • Zavaleta-Mancera H A, Lo´pez-Delgado H, Loza-Tavera H, Mora-Herrera M, Trevilla-Garcı C, Vargas-Sua´rez M and Ougham H (2007). Cytokinin promotes catalase and ascorbate peroxidase activities and preserves the chloroplast integrity during dark-senescence. Journal of Plant Physiology (164) 1572-1582.
  • Zhang H and Zhou C (2013). Signal transduction in leaf senescence. Plant Mol Biol (82) 539-545.
Yıl 2016, Cilt: 33 Sayı: 1, 17 - 24, 16.05.2016
https://doi.org/10.13002/jafag911

Öz

Kaynakça

  • Angelini R, F. Manes and R. Federico (1990). Spatial and functional correlation between diamineoxidase and peroxidase activities and their dependence upon de-etiolation and wounding in chick-pea stems. Planta., 182: 89-96.
  • Arnon D.I (1949). Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol., 24: 1-15.
  • Bartoli C G, Simontacchi M, Guiamet J J, Montaldi E and Puntarulo S (1995). Antioxidant enzymes and lipid peroxidation during aging of Chrysanthemum morifolium RAM petals. Plant Science (104) 161-168.
  • Bazylkoa A, Parzonko A, Jeź W, Osińska E and Kiss A K (2014). Inhibition of ROS production, photoprotection, and total phenolic,flavonoids and ascorbic acid content of fresh herb juice and extractsfrom the leaves and flowers of Tropaeolum majus. Industrial Crops and Products (55) 19–24.
  • Bradford M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-251.
  • Costa M L, Cıvello P M, Chaves A R and Martınez GA (2005). Effect of ethephon and 6-benzylaminopurine on chlorophyll bleaching during Post-Harrest Senescence of Broccoli (Brassica oleracea L.) at 20 0C. Postharvest Biology and Technology, 35: 191-199.
  • Chang C J and Kao C H (1998). H2O2 metabolisim during senesecence of rice leaves:changes in enzyme activities in light and darkness. Plant Growth Regulation (25) 11-15.
  • Das K and Roychoudhury A (2014). Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants. Frontiers in Envıronmental Science. (2) 53. doi: 10.3389/fenvs.2014.00053.
  • Dennis VM and Winfield B A (1978). The determination of starch and cellulose in refuse and compost. Water Poll. Cont., 77: 529-531.
  • Dhindsa R S, Plumb-Dhindsa P L and Reid D M (1982). Leaf senescence and lipid peroxidation: effect of some phytohormones, and scavengers of free radicals and singlet oxygen. Physiol. Plant.(56) 453–457.
  • Downs C G, Somerfıeld S and Davey M C (1997). Cytokinin treatment delays senescence but not sucrose loss in harvested brocolli. Postharv. Biol. Tech (11) 93-100.
  • Duncan B.D (1955). Multiple range and multiple F-tests. Biometrics. P.1-42.
  • Gan S and Amasıno R.M (1995). Inhibition of Leaf Senescence by Autoregulated Production of Cytokinin, Science, 270, 1986–1988
  • Havir E A and Mchale N A (1987). Biochemical and developmental characterization of multiple forms of catalase in tobacco leaves. Plant Physiol., 84: 450-455.
  • Huang J, Han B, Xu S, Zhou M and Shen W (2011). Heme oxygenase-1 is involved in the cytokinin-induced alleviation of senescence in detached wheat leaves during dark incubation. Journal of Plant Physiology (168) 768–775.
  • Jiang W B, Lers A, Lomaniec E and Aharoni N (1999). Senescence-related serine protease in parsley. Phytochemistry (50) 377-382.
  • Jibran R, Hunter D A and Dijkwel P P (2013). Hormonal regulation of leaf senescence through integration of developmental and stress signals. Plant Mol Biol (82)547–561
  • Kanazawa S, Sano S, Koshıba T and Ushımaru T (2000). Changes in antioxidative enzymes in cucumber cotyledons during natural senescence: comparison with those during dark-ınduced senescence, Physiol. Plant. (109) 211-216.
  • Karuppanapandian T, Moon, J-C, Kim C, Manoharan K and Kim W (2011). Reactive oxygen species in plants: their generation, signal transduction, and scavenging mechanisms. AJCS 5, 709-725.
  • Khanna-Chopra R (2012). Leaf senescence and abiotic stresses share reactive oxygen species-mediated chloroplast degradation. Protoplasma (249) 469-481.
  • Li Li Z, Peng J, Wen X and Guo H (2012). Gene network analysis and functional studies of senescence-associated genes reveal novel regulators of Arabidopsis leaf senescence. Journal of Integrative Plant Biology 54 (8): 526–539.
  • Lim O P and Nam G H (2007). Aging and senescence of the leaf organ. Journal of Plant Biology 50 (3): 291-300.
  • Prochazkova D, Saıram R K, Srıvastava G C and Singh D V (2001). Oxidative stres and antioxidant activity as the basis of senescence in maize leaves. Plant Science (161) 765-771.
  • Racchi M L (2013). Antioxidant Defenses in Plants with Attention to Prunus and Citrus spp. Antioxidants (2) 340-369.
  • Ranwala A P and Miller W B (2000). Preventive mechanisms of gibberellin4+7 and light on low-temperature-induced leaf senescence in Lilium cv. Stargazer. Postharvest Biology and Technology, 19 : 85-92.
  • Rogers H J (2012). Is there an important role for reactive oxygen species and redox regulation during floral senescence? Plant, Cell and Environment. (35) 217–233.
  • Rosenvasser S, Mayak S and Friedman H (2006). Increase in reactive oxygen species ( ROS ) and in senescence-associated gene transcript ( SAG ) levels during dark-induced senescence of pelargonium cuttings, and the effect of gibberellic acid. Plant Science (170) 873–879.
  • Rosenwasser S, Belausov E, Riov J, Holdengreber V and Friedman H (2010). Gibberellic acid (GA3) Inhibits ros increase in chloroplasts during dark-ınduced senescence of pelargonium cuttings. J Plant Growth Regul (2010) 29: 375–384.
  • Sairam R K, Singh D V and Srıvastava G C (2003/4). Changes in activities of antioxidant enzymes in sunflower leaves of different ages. Biologia Plantarum,47(1):61-66.
  • Sarwat M, Naqvi A R, Ahmad P, Ashaf M and Akram N A (2013). Phytohormones and microRNAs as sensors and regulators of leaf senescence: Assigning macro roles to small molecules. Biotechnology Advances (31) 1153-1171.
  • Seema, Khokhar M and Mukherjee D (2011). Role of kinetin and a morphactin in leaf disc senescence of Raphanus sativus L. under low light. Physiol Mol Biol Plants 17(3):247–253.
  • Sharma P, Jha A B, Dubey R S and Pessarakli M (2012). Reactive oxygen species, oxidative damage, and antioxidative defensemechanism in plants under stressful conditions. Journal of Botany doi:10.1155/2012/217037.
  • Song Y, Yang C, Gao S, Zhang W, Li L andKuai B (2014). Age-triggered and dark-induced leaf senescence require the bHLH transcription factors PIF3, 4, and 5. Molecular Plant (7) 1776-1787.
  • Velikova V, I. Yordanov and A. Edrava (2000). Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Sci., 151: 59-66.
  • Xu F, Yang Z, Chen X, Jin P, Wang X and Zheng Y (2012). 6-Benzylaminopurine delays senescence and enhances health-promoting compounds of harvested Broccoli. J. Agric. Food Chem. (60) 234−240.
  • Zavaleta-Mancera H A, Lo´pez-Delgado H, Loza-Tavera H, Mora-Herrera M, Trevilla-Garcı C, Vargas-Sua´rez M and Ougham H (2007). Cytokinin promotes catalase and ascorbate peroxidase activities and preserves the chloroplast integrity during dark-senescence. Journal of Plant Physiology (164) 1572-1582.
  • Zhang H and Zhou C (2013). Signal transduction in leaf senescence. Plant Mol Biol (82) 539-545.
Toplam 37 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Araştırma Makaleleri
Yazarlar

Yener Okatan Bu kişi benim

İlhami Karataş

Lokman Öztürk Bu kişi benim

Yayımlanma Tarihi 16 Mayıs 2016
Yayımlandığı Sayı Yıl 2016 Cilt: 33 Sayı: 1

Kaynak Göster

APA Okatan, Y., Karataş, İ., & Öztürk, L. (2016). Karanlıkla Teşvik Edilen Yaprak Senesensi Sürecinde Gibberellik Asit ve 6-Benzilaminopürinin Bazı Biyokimyasal Parametreler Üzerine Etkisi. Journal of Agricultural Faculty of Gaziosmanpaşa University (JAFAG), 33(1), 17-24. https://doi.org/10.13002/jafag911