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Methidathion İnsektisit/Akarisitinin Sitotoksik ve Genotoksik Potansiyelinin Allium Testi ile İncelenmesi

Yıl 2020, Cilt: 1 Sayı: 1-2, 1 - 12, 30.12.2020
https://doi.org/10.5281/zenodo.4317924

Öz

Organik fosforlu pestisitlerden olan Methidathion (Supracide 40 EC, MET), meyve ağaçlarında, sebzelerde,
tütünde, yoncada, ayçiçeğinde, seralarda ve gül bahçelerinde çeşitli böcek ve akarlara karşı
kullanılmaktadır. Bu araştırmada, Allium testi kullanılarak, methidathionun Allium cepa kök ucu
hücrelerindeki sitotoksik ve genotoksik etkileri incelenmiştir. Kök büyüme inhibisyon testi ile, etkili
konsantrasyon (EC50) değeri 30 mg/L olarak tespit edildikten sonra, Allium cepa kök uçları, MET’un dört
farklı konsantrasyonu (7,5, 15, 30 ve 45 mg/L) ile 12, 24 ve 48 saat muamele edilmiştir. MET, mitotik indeksi
(MI), tüm konsantrasyonlarda ve uygulama sürelerinde, kontrole kıyasla anlamlı şekilde düşürmüştür. Diğer
yandan MET, kromozomal anormallikleri, kontrole kıyasla anlamlı düzeyde artırmıştır. En yaygın görülen
anormallikler kroımozom yapışıklığı (%47,50) ve C-mitoz (%44,24)’dur. Bunları sırasıyla fragment (%2,75),
köprü (%2,55), geri kalma (%1,63) ve çok kutupluluk (%1,33) takip etmiştir. Çalışmada ayrıca, ön işleme
tabi tutulmuş kök uçlarında kromozom ve kromatid kırıkları, fragmentler ve poliploidi tespit edilmiştir. Bu
çalışmadan elde edilen sonuçlar, MET’un Allium cepa'da belirgin şekilde sitotoksik ve genotoksik olduğunu
göstermiştir. Ayrıca, Allium testinin, MET’un toksik etkilerini belirlemede çok hassas ve etkili bir test
olduğunu doğrulamıştır.

Kaynakça

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Toplam 71 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Araştırma Makaleleri
Yazarlar

Fatma Ünal Bu kişi benim

Nesrin Durdu Helvacı Tülek Bu kişi benim

Deniz Yüzbaşıoğlu

Mustafa Çelik

Yayımlanma Tarihi 30 Aralık 2020
Yayımlandığı Sayı Yıl 2020 Cilt: 1 Sayı: 1-2

Kaynak Göster

APA Ünal, F., Helvacı Tülek, N. D., Yüzbaşıoğlu, D., Çelik, M. (2020). Methidathion İnsektisit/Akarisitinin Sitotoksik ve Genotoksik Potansiyelinin Allium Testi ile İncelenmesi. Gazi Üniversitesi Fen Fakültesi Dergisi, 1(1-2), 1-12. https://doi.org/10.5281/zenodo.4317924
AMA Ünal F, Helvacı Tülek ND, Yüzbaşıoğlu D, Çelik M. Methidathion İnsektisit/Akarisitinin Sitotoksik ve Genotoksik Potansiyelinin Allium Testi ile İncelenmesi. GÜFFD. Aralık 2020;1(1-2):1-12. doi:10.5281/zenodo.4317924
Chicago Ünal, Fatma, Nesrin Durdu Helvacı Tülek, Deniz Yüzbaşıoğlu, ve Mustafa Çelik. “Methidathion İnsektisit/Akarisitinin Sitotoksik Ve Genotoksik Potansiyelinin Allium Testi Ile İncelenmesi”. Gazi Üniversitesi Fen Fakültesi Dergisi 1, sy. 1-2 (Aralık 2020): 1-12. https://doi.org/10.5281/zenodo.4317924.
EndNote Ünal F, Helvacı Tülek ND, Yüzbaşıoğlu D, Çelik M (01 Aralık 2020) Methidathion İnsektisit/Akarisitinin Sitotoksik ve Genotoksik Potansiyelinin Allium Testi ile İncelenmesi. Gazi Üniversitesi Fen Fakültesi Dergisi 1 1-2 1–12.
IEEE F. Ünal, N. D. Helvacı Tülek, D. Yüzbaşıoğlu, ve M. Çelik, “Methidathion İnsektisit/Akarisitinin Sitotoksik ve Genotoksik Potansiyelinin Allium Testi ile İncelenmesi”, GÜFFD, c. 1, sy. 1-2, ss. 1–12, 2020, doi: 10.5281/zenodo.4317924.
ISNAD Ünal, Fatma vd. “Methidathion İnsektisit/Akarisitinin Sitotoksik Ve Genotoksik Potansiyelinin Allium Testi Ile İncelenmesi”. Gazi Üniversitesi Fen Fakültesi Dergisi 1/1-2 (Aralık 2020), 1-12. https://doi.org/10.5281/zenodo.4317924.
JAMA Ünal F, Helvacı Tülek ND, Yüzbaşıoğlu D, Çelik M. Methidathion İnsektisit/Akarisitinin Sitotoksik ve Genotoksik Potansiyelinin Allium Testi ile İncelenmesi. GÜFFD. 2020;1:1–12.
MLA Ünal, Fatma vd. “Methidathion İnsektisit/Akarisitinin Sitotoksik Ve Genotoksik Potansiyelinin Allium Testi Ile İncelenmesi”. Gazi Üniversitesi Fen Fakültesi Dergisi, c. 1, sy. 1-2, 2020, ss. 1-12, doi:10.5281/zenodo.4317924.
Vancouver Ünal F, Helvacı Tülek ND, Yüzbaşıoğlu D, Çelik M. Methidathion İnsektisit/Akarisitinin Sitotoksik ve Genotoksik Potansiyelinin Allium Testi ile İncelenmesi. GÜFFD. 2020;1(1-2):1-12.