Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris

Murat Ozturk; Ali Endes

doi:10.29050/harranziraat.1216304

Araştırma Makalesi

Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris

Yıl 2023, Cilt: 27 Sayı: 1, 64 - 72, 24.03.2023

Murat Ozturk Ali Endes

https://doi.org/10.29050/harranziraat.1216304

Cited By: 1

Öz

In 2021, post-harvest soil samples were taken from fields where chickpea and wheat were grown in Yozgat province to isolate bacteria. Of 74 isolates obtained from soil samples, 14 nonpathogenic isolates were evaluated for their inhibitory potential against two pathogenic isolates (YBUFoc9 and YBUFoc2) of Fusarium wilt disease agent Fusarium oxysporum f. sp. ciceris. Antagonistic bacterial isolates were identified as Bacillus spp. by Blastn analysis based on their 16S rDNA nucleotide sequences. Among them, 2 isolates were identified as B. amyloliquefaciens (BM23 and BM40), 3 isolates as B. subtilis (BM8, BM32, and BM105), 6 isolates as B. cereus (BM10, BM69, BM70, BM104, BM111 and BM215), 2 isolates as B. megaterium (BM44 and BM135) and 1 isolate as B. pumilus (BM28). No effective isolate of any genus other than Bacillus spp. was found. They inhibited mycelial growth of isolates YBUFoc9 and YBUFoc2 from 0 to 78.50% and from 0 to 58.33%, respectively. Among the 5 different species, B. amyloliquefaciens (BM23 and BM40) and B. subtilis (BM8, BM32, and BM105) showed the strongest inhibitory activity against the two fungal isolates under in vitro conditions. In further studies, the biocontrol properties of the most effective isolates (BM23, BM40, BM8, BM32 and BM105) will be investigated and compared with approved fungicides for their fungicidal activity.

Anahtar Kelimeler

Fusarium wilt, Chickpea, Bacillus spp., Biological control

Teşekkür

We would like to thank Prof. Dr. Soner Soylu for valuable advice on experimental design and important criticisms of the manuscript and Dr. Ali Kayahan for statistical analysis.

Kaynakça

Abed, H., Rouag, N., Mouatassem, D., & Rouabhi, A. (2016). Screening for Pseudomonas and Bacillus antagonistic rhizobacteria strains for the biocontrol of Fusarium wilt of chickpea. Eurasian Journal of Soil Science, 5(3), 182-191. DOI: https://doi.org/10.3906/biy-0809-12
Abbas, A., Khan, S. U., Khan, W. U., Saleh, T. A., Khan, M. H. U., Ullah, S., Ali, A., & Ikram, M. (2019). Antagonist effects of strains of Bacillus spp. against Rhizoctonia solani for their protection against several plant diseases: Alternatives to chemical pesticides. Comptes Rendus Biologies, 342(5-6), 124-135. DOI: https://doi.org/10.1016/j.crvi.2019.05.002
Akhtar, M. S., Shakeel, U., & Siddiqui, Z. A. (2010). Biocontrol of Fusarium wilt by Bacillus pumilus, Pseudomonas alcaligenes and Rhizobium sp. on lentil. Turkish Journal of Biology, 34(1), 1-7. DOI: 10.3906/biy-0809-12.
Aksoy, H.M., Kaya, Y., Ozturk, M., Secgin, Z., Onder, H., & Okumus, A. (2017). Pseudomonas putida–Induced response in phenolic profile of tomato seedlings (Solanum lycopersicum L.) infected by Clavibacter michiganensis subsp. michiganensis. Biological Control, 105, 6–12.https://doi.org/10.1016/j.biocontrol.2016.11.001
Aktan, Z.C., & Soylu, S. (2020). Diyarbakır ilinde yetişen badem ağaçlarından endofit ve epifit bakteri türlerinin izolasyonu ve bitki gelişimini teşvik eden mekanizmalarının karakterizasyonu. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23 (3), 641-654.
Aydın, M. H. (2019). The biological control of Fusarium oxysporum causing wilting in chickpea (Cicer arietinum L.). Türkiye Tarımsal Araștırmalar Dergisi, 6(1), 65-72. DOI: https://doi.org/10.19159/tutad.475915
Akgün Yıldırım, Ü., & Güldür, M.E. (2019). Tescilli nohut çeşitlerinde Fusarium dayanıklılığının belirlenmesi. Harran Tarım ve Gıda Bilimleri Dergisi, 23(2): 218-225. DOI: https://doi.org/10.29050/harranziraat.461816
Anonymous (2019a). TUİK Bitkisel Üretim İstatistikleri https://biruni.tuik.gov.tr/medas/?kn=92&locale=tr, (Retrieved 20.04.2021)
Anonymous (2019b). Food and Agriculture Organization of the United Nations (FAO), http://www.fao.org/site, (Retrieved 20.04.2021).
Anonymous (2021) Tarimsal Ekonomi ve Politika Geliştirme Enstitüsü TEPGE, Ürün Raporu: Nohuthttps://arastirma.tarimorman.gov.tr/tepge/Belgeler/PDF%20%C3%9Cr%C3%BCn%20Raporlar%C4%B1/2021%20%C3%9Cr%C3%BCn%20Raporlar%C4%B1/Nohut%20%C3%9Cr%C3%BCn%20Raporu%202021-342%20TEPGE.pdf. (Retrieved 08.04.2022).
Bayraktar, H., & Dolar, F. S. (2012). Pathogenic variability of Fusarium oxysporum f. sp. ciceris isolates from chickpea in Turkey. Pakistan Journal of Botany. 44(2): 821-823.
Edwards, C. A., & Arancon, N. Q. (2004). Interactions among organic matter, earthworms, and microorganisms in promoting plant growth. Soil organic matter in sustainable agriculture, 327-376.
Elbouazaoui, A., Sijilmassi, B., Maafa, I., Allal, D., & Ahmed, S. (2022). Biocontrol activity of Bacillus, Paenibacillus and Pseudomonas against Fusarium wilt of chickpea in Morocco, Acta Agriculturae Scandinavica, Section B — Soil & Plant Science, 72:1, 847-859, DOI: https://doi.org/10.1080/09064710.2022.2100819
Guerrero-Aguilar, B. Z., Acosta-Gallegos, J. A., Sánchez-García, B. M., Ortega-Murrieta, P. F., & González-Chavira, M. M. (2015). Razas patogénicas de Fusarium oxysporum f. sp. ciceris en garbanzo cultivado en Guanajuato, México. Revista Fitotecnia Mexicana, 38(2), 183-190.
Houasli, C., Sahri, A., Nsarellah, N., & Idrissi, O. (2021). Chickpea (Cicer arietinum L.) breeding in Morocco: genetic gain and stability of grain yield and seed size under winter planting conditions. Euphytica, 217(8), 1-14. DOI: https://doi.org/10.1007/s10681-021-02885-x
Jamali, F., Sharifi-Tehrani, A., Okhovvat, M., Zakeri, Z., & Saberi-Riseh, R. (2004). Biological control of chickpea Fusarium wilt by antagonistic bacteria under greenhouse condition. Communications in agricultural and applied biological sciences, 69(4), 649-651.
Jiménez-Díaz, R. M., Castillo, P., del Mar Jiménez-Gasco, M., Landa, B. B., & Navas-Cortés, J. A. (2015). Fusarium wilt of chickpeas: Biology, ecology and management. Crop Protection, 73, 16-27. DOI: https://doi.org/10.1016/j.cropro.2015.02.023
Johri, B. N., Sharma, A., & Virdi, J. S. (2003). Rhizobacterial Diversity in India and Its Influence on Soil and Plant Health. Advances in Biochemical Engineering/Biotechnology, 84, 49-89. DOI: https://doi.org/10.1007/3-540-36488-9_2
Kara, M., Soylu, S., Kurt, Ş., Soylu, E.M., & Uysal, A. (2020). Determination of antagonistic traits of bacterial isolates obtained from apricot against green fruit rot disease agent Sclerotinia sclerotiorum. Acta Horticulturae, 1290:135-142.
Kara, M., & Soylu, S. (2022). Isolation of endophytic bacterial isolates from healthy banana trees and determination of their in vitro antagonistic activities against crown rot disease agent Fusarium verticillioides. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 27(1), 36-46.
Kocalar, H., Kafadar, F.N., Ozkan, A., Talapov, T., Demirel, O., Anay, A., Mart, D., & Can, C. (2020). Current Distribution and Virulence of Fusarium oxysporum f. sp. ciceris in Turkey. Legume Research, 43(5): 735-741. DOI: https://doi.org/10.18805/LR-520
Khan, M. R., Ashraf, S., Rasool, F., SALATI, K., Mohiddin, F. A., & Haque, Z. (2014). Field performance of Trichoderma species against wilt disease complex of chickpea caused by Fusarium oxysporum f. sp. ciceri and Rhizoctonia solani. Turkish Journal of Agriculture and Forestry, 38(4), 447-454. DOI: https://doi.org/10.3906/tar-1209-10
Landa, B. B., Navas‐Cortés, J. A., & Jiménez‐Díaz, R. M. (2004). Influence of temperature on plant–rhizobacteria interactions related to biocontrol potential for suppression of Fusarium wilt of chickpea. Plant Pathology, 53(3), 341-352. DOI: https://doi.org/10.1111/j.0032-0862.2004.01008.x.
Lelliot, R.A., & Stead, D.E. (1987). Methods for the diagnosis of bacterial diseases of plants. (T.F. Preece. Editör). In: Methods in plant pathology. Vol 2. Blackwell Scientific Publications, pp. 176-177. Oxford.
Leslie, J.F., & Summerell, B.A. (2006). The Fusarium laboratory Manual Blackwell Publishing. Ames, Iowa, 230-239.
Muehlbauer, F.J., & Sarker, A. (2017). Economic importance of chickpea: production, value, and world trade. In: Varshney RK, Thudi M, Muehlbauer F, editors. The chickpea genome. Springer; p. 5–12. DOI: https://doi.org/10.1007/978-3-319-66117-9_2.
Nene, Y.L., & Haware, M.P. (1980). Screening chickpea for resistance to wilt. Plant Disease, 66, 379-380. Nelson, P. E., Toussoun, T.A., & Marasas, W.F.O. (1983). Fusarium species: an illustrated manual for identification. Pennsylvania State University Press, University Park. 193 pp.
Oliva-Ortiz, L. D. C., Velázquez-Alcaraz, T. D. J., Sosa-Pérez, R., Partida-Ruvalcaba, L., Díaz-Valdés, T., Arciniega-Ramos, J., & López-Orona, C. A. (2017). Controlling Fusarium wilt of chickpea (Cicer arietinum L.) with native microorganisms of Sinaloa, Mexico. Agrociencia, 51(6), 683-695.
Öztürk M., & Soylu, S. (2022). Yozgat ve Kırşehir illerinde tüketime sunulmuş patates yumrularında bakteriyel yumuşak çürüklük etmeni Pectobacterium izolatlarının izolasyonu ve tanılanması. Tekirdağ Ziraat Fakültesi Dergisi, 19 (2), 332-342. DOI: https://doi.org/10.33462/jotaf.974350
Özkaya S, & Soylu, E.M. (2022). Kompostlardan elde edilen antagonist bakteri izolatlarının kavun solgunluk hastalığı etmeni Fusarium oxysporum f.sp. melonis’e karşı in vitro antagonistik etkilerinin belirlenmesi. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 27(3), 565-577. DOI: https://doi.org/10.37908/mkutbd.1139502
Özyılmaz, Ü. (2019). Biberde Phytophthora Yanıklığına karşı antagonist bakterilerle biyolojik mücadele. Yuzuncu Yıl University Journal of Agricultural Sciences, 29(4), 652-661. DOI: https://doi.org/10.29133/yyutbd.597443
Rangel-Montoya, E. A., Delgado-Ramírez, C. S., Sepulveda, E., & Hernández-Martínez, R. (2022). Biocontrol of Macrophomina phaseolina using Bacillus amyloliquefaciens strains in cowpea (Vigna unguiculata L.). Agronomy, 12(3), 676. DOI: https://doi.org/10.3390/agronomy12030676
Soylu, E.M., Soylu, S., Kara, M., Kurt, Ş. (2020). Sebzelerde sorun olan önemli bitki fungal hastalık etmenlerine karşı vermikomposttan izole edilen mikrobiyomların in vitro antagonistik etkilerinin belirlenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23 (1), 7-18. DOI: https://doi.org/10.3390/agronomy12030676
Soylu, S., Kara, M., Soylu, E.M ., Uysal, A., & Kurt, Ş. (2022). Geotrichum citri-aurantii’nin sebep olduğu turunçgil ekşi çürüklük hastalığının biyolojik mücadelesinde endofit bakterilerin biyokontrol potansiyellerinin belirlenmesi. Tekirdağ Ziraat Fakültesi Dergisi, 19 (1), 177-191.
Villarreal-Delgado, M. F., Villa-Rodríguez, E. D., Cira-Chávez, L. A., Estrada-Alvarado, M. I., Parra-Cota, F. I., & Santos-Villalobos, S. D. L. (2018). The genus Bacillus as a biological control agent and its implications in the agricultural biosecurity. Revista Mexicana de Fitopatología, 36(1), 95-130. DOI: https://doi.org/10.18781/r.mex.fit.1706-5
Zaim, S., Belabid, L., Bayaa, B., & Bekkar, A. A. (2016). Biological control of chickpea Fusarium wilts using rhizobacteria “PGPR”. In Microbial-mediated Induced Systemic Resistance in Plants (pp. 147-162). Springer, Singapore. DOI: https://doi.org/10.1007/978-981-10-0388-2_10

Toprak örneklerinden bakteri izolatlarının izolasyonu ve nohut solgunluğu hastalığına neden olan Fusarium oxysporum f. sp. ciceris’e karşı in vitro antagonistik potansiyellerinin belirlenmesi

Yıl 2023, Cilt: 27 Sayı: 1, 64 - 72, 24.03.2023

Murat Ozturk Ali Endes

https://doi.org/10.29050/harranziraat.1216304

Cited By: 1

Öz

2021 yılında Yozgat ilinde nohut ve buğday yetiştirilen tarlalardan bakteri izolasyonu için hasat sonrası toprak örnekleri toplanmıştır. Toprak örneklerinden izole edilen 74 izolat arasından patojenik olmayan 14 aday bakteri izolatın Fusarium solgunluğu etmeni Fusarium oxysporum f. sp. ciceris’in iki izolatına (YBUFoc9 and YBUFoc2) karşı engelleme potansiyelleri in vitro koşullarda değerlendirilmiştir. Antagonist bakteri izolatların tanısı 16S rDNA nükleotid dizilerine dayanan Blastn analizi ile Bacillus spp. olarak belirlenmiştir. Elde edilen izolatların 2'si B. amyloliquefaciens (BM23 ve BM40), 3'ü B. subtilis (BM8, BM32 ve BM105), 6'sı B. cereus (BM10, BM69, BM70, BM104, BM111 ve BM215), 2’si B. megaterium olarak (BM44 ve BM135) ve 1’i B. pumilus (BM28) olarak tanılanmıştır. Antagonistik etkinlik bakımından Bacillus spp. dışında etkili bir cinse rastlanılmamıştır. Bakteriyel izolatlar, YBUFoc9 ve YBUFoc2 izolatlarının misel büyümesine sırasıyla %0-%78.50 ve %0-%58.33 oranında inhibisyon sağlamışlardır. Beş farklı tür arasından B. amyloliquefaciens (BM23 ve BM40) ve B. subtilis’in (BM8, BM32 ve BM105) in vitro koşullar altında patojenin iki izolatına karşı en güçlü inhibisyon aktivitesi gösterdiği belirlenmiştir. Gelecekte yapılması planlanan çalışmalarda, en etkili izolatların (BM23, BM40, BM8, BM32 ve BM105) biyokontrol özellikleri araştırılacak ve fungisidal aktiviteleri için onaylanmış fungisitlerle karşılaştırılacaktır.

Anahtar Kelimeler

Fusarium solgunluğu, Nohut, Bacillus spp., Biyolojik mücadele

Kaynakça

Abed, H., Rouag, N., Mouatassem, D., & Rouabhi, A. (2016). Screening for Pseudomonas and Bacillus antagonistic rhizobacteria strains for the biocontrol of Fusarium wilt of chickpea. Eurasian Journal of Soil Science, 5(3), 182-191. DOI: https://doi.org/10.3906/biy-0809-12
Abbas, A., Khan, S. U., Khan, W. U., Saleh, T. A., Khan, M. H. U., Ullah, S., Ali, A., & Ikram, M. (2019). Antagonist effects of strains of Bacillus spp. against Rhizoctonia solani for their protection against several plant diseases: Alternatives to chemical pesticides. Comptes Rendus Biologies, 342(5-6), 124-135. DOI: https://doi.org/10.1016/j.crvi.2019.05.002
Akhtar, M. S., Shakeel, U., & Siddiqui, Z. A. (2010). Biocontrol of Fusarium wilt by Bacillus pumilus, Pseudomonas alcaligenes and Rhizobium sp. on lentil. Turkish Journal of Biology, 34(1), 1-7. DOI: 10.3906/biy-0809-12.
Aksoy, H.M., Kaya, Y., Ozturk, M., Secgin, Z., Onder, H., & Okumus, A. (2017). Pseudomonas putida–Induced response in phenolic profile of tomato seedlings (Solanum lycopersicum L.) infected by Clavibacter michiganensis subsp. michiganensis. Biological Control, 105, 6–12.https://doi.org/10.1016/j.biocontrol.2016.11.001
Aktan, Z.C., & Soylu, S. (2020). Diyarbakır ilinde yetişen badem ağaçlarından endofit ve epifit bakteri türlerinin izolasyonu ve bitki gelişimini teşvik eden mekanizmalarının karakterizasyonu. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23 (3), 641-654.
Aydın, M. H. (2019). The biological control of Fusarium oxysporum causing wilting in chickpea (Cicer arietinum L.). Türkiye Tarımsal Araștırmalar Dergisi, 6(1), 65-72. DOI: https://doi.org/10.19159/tutad.475915
Akgün Yıldırım, Ü., & Güldür, M.E. (2019). Tescilli nohut çeşitlerinde Fusarium dayanıklılığının belirlenmesi. Harran Tarım ve Gıda Bilimleri Dergisi, 23(2): 218-225. DOI: https://doi.org/10.29050/harranziraat.461816
Anonymous (2019a). TUİK Bitkisel Üretim İstatistikleri https://biruni.tuik.gov.tr/medas/?kn=92&locale=tr, (Retrieved 20.04.2021)
Anonymous (2019b). Food and Agriculture Organization of the United Nations (FAO), http://www.fao.org/site, (Retrieved 20.04.2021).
Anonymous (2021) Tarimsal Ekonomi ve Politika Geliştirme Enstitüsü TEPGE, Ürün Raporu: Nohuthttps://arastirma.tarimorman.gov.tr/tepge/Belgeler/PDF%20%C3%9Cr%C3%BCn%20Raporlar%C4%B1/2021%20%C3%9Cr%C3%BCn%20Raporlar%C4%B1/Nohut%20%C3%9Cr%C3%BCn%20Raporu%202021-342%20TEPGE.pdf. (Retrieved 08.04.2022).
Bayraktar, H., & Dolar, F. S. (2012). Pathogenic variability of Fusarium oxysporum f. sp. ciceris isolates from chickpea in Turkey. Pakistan Journal of Botany. 44(2): 821-823.
Edwards, C. A., & Arancon, N. Q. (2004). Interactions among organic matter, earthworms, and microorganisms in promoting plant growth. Soil organic matter in sustainable agriculture, 327-376.
Elbouazaoui, A., Sijilmassi, B., Maafa, I., Allal, D., & Ahmed, S. (2022). Biocontrol activity of Bacillus, Paenibacillus and Pseudomonas against Fusarium wilt of chickpea in Morocco, Acta Agriculturae Scandinavica, Section B — Soil & Plant Science, 72:1, 847-859, DOI: https://doi.org/10.1080/09064710.2022.2100819
Guerrero-Aguilar, B. Z., Acosta-Gallegos, J. A., Sánchez-García, B. M., Ortega-Murrieta, P. F., & González-Chavira, M. M. (2015). Razas patogénicas de Fusarium oxysporum f. sp. ciceris en garbanzo cultivado en Guanajuato, México. Revista Fitotecnia Mexicana, 38(2), 183-190.
Houasli, C., Sahri, A., Nsarellah, N., & Idrissi, O. (2021). Chickpea (Cicer arietinum L.) breeding in Morocco: genetic gain and stability of grain yield and seed size under winter planting conditions. Euphytica, 217(8), 1-14. DOI: https://doi.org/10.1007/s10681-021-02885-x
Jamali, F., Sharifi-Tehrani, A., Okhovvat, M., Zakeri, Z., & Saberi-Riseh, R. (2004). Biological control of chickpea Fusarium wilt by antagonistic bacteria under greenhouse condition. Communications in agricultural and applied biological sciences, 69(4), 649-651.
Jiménez-Díaz, R. M., Castillo, P., del Mar Jiménez-Gasco, M., Landa, B. B., & Navas-Cortés, J. A. (2015). Fusarium wilt of chickpeas: Biology, ecology and management. Crop Protection, 73, 16-27. DOI: https://doi.org/10.1016/j.cropro.2015.02.023
Johri, B. N., Sharma, A., & Virdi, J. S. (2003). Rhizobacterial Diversity in India and Its Influence on Soil and Plant Health. Advances in Biochemical Engineering/Biotechnology, 84, 49-89. DOI: https://doi.org/10.1007/3-540-36488-9_2
Kara, M., Soylu, S., Kurt, Ş., Soylu, E.M., & Uysal, A. (2020). Determination of antagonistic traits of bacterial isolates obtained from apricot against green fruit rot disease agent Sclerotinia sclerotiorum. Acta Horticulturae, 1290:135-142.
Kara, M., & Soylu, S. (2022). Isolation of endophytic bacterial isolates from healthy banana trees and determination of their in vitro antagonistic activities against crown rot disease agent Fusarium verticillioides. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 27(1), 36-46.
Kocalar, H., Kafadar, F.N., Ozkan, A., Talapov, T., Demirel, O., Anay, A., Mart, D., & Can, C. (2020). Current Distribution and Virulence of Fusarium oxysporum f. sp. ciceris in Turkey. Legume Research, 43(5): 735-741. DOI: https://doi.org/10.18805/LR-520
Khan, M. R., Ashraf, S., Rasool, F., SALATI, K., Mohiddin, F. A., & Haque, Z. (2014). Field performance of Trichoderma species against wilt disease complex of chickpea caused by Fusarium oxysporum f. sp. ciceri and Rhizoctonia solani. Turkish Journal of Agriculture and Forestry, 38(4), 447-454. DOI: https://doi.org/10.3906/tar-1209-10
Landa, B. B., Navas‐Cortés, J. A., & Jiménez‐Díaz, R. M. (2004). Influence of temperature on plant–rhizobacteria interactions related to biocontrol potential for suppression of Fusarium wilt of chickpea. Plant Pathology, 53(3), 341-352. DOI: https://doi.org/10.1111/j.0032-0862.2004.01008.x.
Lelliot, R.A., & Stead, D.E. (1987). Methods for the diagnosis of bacterial diseases of plants. (T.F. Preece. Editör). In: Methods in plant pathology. Vol 2. Blackwell Scientific Publications, pp. 176-177. Oxford.
Leslie, J.F., & Summerell, B.A. (2006). The Fusarium laboratory Manual Blackwell Publishing. Ames, Iowa, 230-239.
Muehlbauer, F.J., & Sarker, A. (2017). Economic importance of chickpea: production, value, and world trade. In: Varshney RK, Thudi M, Muehlbauer F, editors. The chickpea genome. Springer; p. 5–12. DOI: https://doi.org/10.1007/978-3-319-66117-9_2.
Nene, Y.L., & Haware, M.P. (1980). Screening chickpea for resistance to wilt. Plant Disease, 66, 379-380. Nelson, P. E., Toussoun, T.A., & Marasas, W.F.O. (1983). Fusarium species: an illustrated manual for identification. Pennsylvania State University Press, University Park. 193 pp.
Oliva-Ortiz, L. D. C., Velázquez-Alcaraz, T. D. J., Sosa-Pérez, R., Partida-Ruvalcaba, L., Díaz-Valdés, T., Arciniega-Ramos, J., & López-Orona, C. A. (2017). Controlling Fusarium wilt of chickpea (Cicer arietinum L.) with native microorganisms of Sinaloa, Mexico. Agrociencia, 51(6), 683-695.
Öztürk M., & Soylu, S. (2022). Yozgat ve Kırşehir illerinde tüketime sunulmuş patates yumrularında bakteriyel yumuşak çürüklük etmeni Pectobacterium izolatlarının izolasyonu ve tanılanması. Tekirdağ Ziraat Fakültesi Dergisi, 19 (2), 332-342. DOI: https://doi.org/10.33462/jotaf.974350
Özkaya S, & Soylu, E.M. (2022). Kompostlardan elde edilen antagonist bakteri izolatlarının kavun solgunluk hastalığı etmeni Fusarium oxysporum f.sp. melonis’e karşı in vitro antagonistik etkilerinin belirlenmesi. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 27(3), 565-577. DOI: https://doi.org/10.37908/mkutbd.1139502
Özyılmaz, Ü. (2019). Biberde Phytophthora Yanıklığına karşı antagonist bakterilerle biyolojik mücadele. Yuzuncu Yıl University Journal of Agricultural Sciences, 29(4), 652-661. DOI: https://doi.org/10.29133/yyutbd.597443
Rangel-Montoya, E. A., Delgado-Ramírez, C. S., Sepulveda, E., & Hernández-Martínez, R. (2022). Biocontrol of Macrophomina phaseolina using Bacillus amyloliquefaciens strains in cowpea (Vigna unguiculata L.). Agronomy, 12(3), 676. DOI: https://doi.org/10.3390/agronomy12030676
Soylu, E.M., Soylu, S., Kara, M., Kurt, Ş. (2020). Sebzelerde sorun olan önemli bitki fungal hastalık etmenlerine karşı vermikomposttan izole edilen mikrobiyomların in vitro antagonistik etkilerinin belirlenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23 (1), 7-18. DOI: https://doi.org/10.3390/agronomy12030676
Soylu, S., Kara, M., Soylu, E.M ., Uysal, A., & Kurt, Ş. (2022). Geotrichum citri-aurantii’nin sebep olduğu turunçgil ekşi çürüklük hastalığının biyolojik mücadelesinde endofit bakterilerin biyokontrol potansiyellerinin belirlenmesi. Tekirdağ Ziraat Fakültesi Dergisi, 19 (1), 177-191.
Villarreal-Delgado, M. F., Villa-Rodríguez, E. D., Cira-Chávez, L. A., Estrada-Alvarado, M. I., Parra-Cota, F. I., & Santos-Villalobos, S. D. L. (2018). The genus Bacillus as a biological control agent and its implications in the agricultural biosecurity. Revista Mexicana de Fitopatología, 36(1), 95-130. DOI: https://doi.org/10.18781/r.mex.fit.1706-5
Zaim, S., Belabid, L., Bayaa, B., & Bekkar, A. A. (2016). Biological control of chickpea Fusarium wilts using rhizobacteria “PGPR”. In Microbial-mediated Induced Systemic Resistance in Plants (pp. 147-162). Springer, Singapore. DOI: https://doi.org/10.1007/978-981-10-0388-2_10

Toplam 36 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	İngilizce
Konular	Bitki Bilimi, Ziraat Mühendisliği
Bölüm	Araştırma Makaleleri
Yazarlar	Murat Ozturk 0000-0002-9677-3651 Ali Endes 0000-0003-4815-5864
Yayımlanma Tarihi	24 Mart 2023
Gönderilme Tarihi	8 Aralık 2022
Yayımlandığı Sayı	Yıl 2023 Cilt: 27 Sayı: 1

Kaynak Göster

APA	Ozturk, M., & Endes, A. (2023). Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris. Harran Tarım Ve Gıda Bilimleri Dergisi, 27(1), 64-72. https://doi.org/10.29050/harranziraat.1216304