Araştırma Makalesi
Yıl 2020,
Cilt: 4 Sayı: 2, 161 - 166, 30.12.2020
Öz
In this study, in vitro and in vivo seed germination capacity of zinnia (Zinnia elegans L.), mostly grown as seasonal ornamental plant and the effects of different plant growth regulators on shoot regeneration and callus formation of different explants (leaf, leaf blade, leaf stalk) were investigated. The best surface sterilization of the seeds was achieved in 5% sodium hypochlorite solution for 3 min. The greatest germinations (25-30%) were observed in in vivo media. Among the in vitro germination media, MS medium yielded the worst outcomes and it was followed by White medium. Germination started five days after sowing, continued between the 5th and 7th days and stopped after the 7th day. In terms of callus formation, tillering and shoot regeneration, MS medium yielded the best outcomes. Leaf explants were identified as the best source of explant. The best callus formation (2.67%) and shoot regeneration (2.33%) ratios were obtained from 1mg/L 2.4-D + 1mg/L BA containing MS medium. It was followed by 1mg/L 2.4-D + 1mg/L BA containing B5 medium with shoot regeneration ratio of 2.25% and callus formation ratio of 1.13%.
Anahtar Kelimeler
Destekleyen Kurum
Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi
Proje Numarası
FYL-2017-5623
Teşekkür
This study was supported by Scientific Research Projects Department of Van Yuzuncu University (with the project number of FYL-2017-5623).
Kaynakça
- Ay S, 2009. Ornamental Plant Export, Problems and Solution Proposals: The Case of Yalova. Süleyman Demirel University Journal of Faculty of economics and Administrative Sciences. 14:423-443 (in Turkish).
- Gamborg O.L., Miller R.A., Ojima O. 1968. Nutrient requirements of suspension cultures of soybean root cell. Exp. Cell Res. 50: 151-158.
- Hatipoğlu, R., 1997. Plant Biotechnology. Çukurova University Agricultural Faculty, Publication No:190, 176p, Adana (in Turkish).
- Karagüzel, O., Korkut, A.B., Özkan, B., Çelikel, F.G., Titiz, S.,2010. Current Status of Ornamental Plant Production, Potential of Development and Targets, Agricultural Engineering VII. Technical Congress, 11-15 January, Ankara (in Turkish).
- Kayhan, E., Baran, M. F., 2014. Economic and technical comparison of plow and heavy-spring cultivar in sunflower farming under dry conditions. Journal of Turkish Agriculture and Natural Sciences, 1(4): 435-440 (in Turkish).
- Mahmoodzadeh, F. H. Abbasi and S. Rohani .,2010 In vitro Germination and Early Seedling Growth of Zinnia elegans, Research Journal of Environmental Sciences 4 (4): 407-413.
- Murashige, T. and Skoog, F., 1962, A resived medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant., 15: 473-497.
- Özdemir, F.A., Sağlam., Yıldırım, M.U.,2014. Effects of Different Plant Growth Regulators on In Vitro germination of different originated Fagus (Fagus orientalis Lipsky.) Seeds. Agricultural Sciences Research Journal, 7 (1): 62-64 (in Turkish).
- Rogers, M.N., Tjia, B.O. (1990) Gerbera production for cut flowers and pot plants. Timber Press, Portland, OR.
- Sayın, B. Sayın, C., 2004. Assessment of Turkish Ornamental Plant Production and Marketing Structure in terms of EU Compliance. VI. Turkish Agricultural Economics Congress, 16-18 September, Tokat (in Turkish).
- Tandokazi, P., Magangana, Marietjie, A. Stander, Nokwanda, P., Makunga., 2018. Effect of nitrogen and phosphate on in vitro growth and metabolite profiles of Stevia rebaudiana Bertoni (Asteraceae). Plant Cell, Tissue and Organ Culture (PCTOC) 134:141–151.
- Trejgell,a., Kamıńska,M., Lısowska,K., Tretyn,A.,2018. Not Bot Horti Agrobo, 46(1):52-57. DOI:10.15835/nbha46110810).
- Valletta, A., A. R. Santamarıa, A, R., Fabrını, G., Toccı, N., Fılho, V. C., Wagner, T., Brasılı, E., Pasqua, G., 2016. Strategies for ex situ conservation of Centaurea cineraria subsp. circae (Asteraceae), an endemic plant from Lazio (Italy). Plant Biosystems, Vol. 150, No. 2, 323–332.
- White P.R. 1963. The cultivation of Animal and Plant Cells. Ronald Press, New York.
Yıl 2020,
Cilt: 4 Sayı: 2, 161 - 166, 30.12.2020
Öz
Proje Numarası
FYL-2017-5623
Kaynakça
- Ay S, 2009. Ornamental Plant Export, Problems and Solution Proposals: The Case of Yalova. Süleyman Demirel University Journal of Faculty of economics and Administrative Sciences. 14:423-443 (in Turkish).
- Gamborg O.L., Miller R.A., Ojima O. 1968. Nutrient requirements of suspension cultures of soybean root cell. Exp. Cell Res. 50: 151-158.
- Hatipoğlu, R., 1997. Plant Biotechnology. Çukurova University Agricultural Faculty, Publication No:190, 176p, Adana (in Turkish).
- Karagüzel, O., Korkut, A.B., Özkan, B., Çelikel, F.G., Titiz, S.,2010. Current Status of Ornamental Plant Production, Potential of Development and Targets, Agricultural Engineering VII. Technical Congress, 11-15 January, Ankara (in Turkish).
- Kayhan, E., Baran, M. F., 2014. Economic and technical comparison of plow and heavy-spring cultivar in sunflower farming under dry conditions. Journal of Turkish Agriculture and Natural Sciences, 1(4): 435-440 (in Turkish).
- Mahmoodzadeh, F. H. Abbasi and S. Rohani .,2010 In vitro Germination and Early Seedling Growth of Zinnia elegans, Research Journal of Environmental Sciences 4 (4): 407-413.
- Murashige, T. and Skoog, F., 1962, A resived medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant., 15: 473-497.
- Özdemir, F.A., Sağlam., Yıldırım, M.U.,2014. Effects of Different Plant Growth Regulators on In Vitro germination of different originated Fagus (Fagus orientalis Lipsky.) Seeds. Agricultural Sciences Research Journal, 7 (1): 62-64 (in Turkish).
- Rogers, M.N., Tjia, B.O. (1990) Gerbera production for cut flowers and pot plants. Timber Press, Portland, OR.
- Sayın, B. Sayın, C., 2004. Assessment of Turkish Ornamental Plant Production and Marketing Structure in terms of EU Compliance. VI. Turkish Agricultural Economics Congress, 16-18 September, Tokat (in Turkish).
- Tandokazi, P., Magangana, Marietjie, A. Stander, Nokwanda, P., Makunga., 2018. Effect of nitrogen and phosphate on in vitro growth and metabolite profiles of Stevia rebaudiana Bertoni (Asteraceae). Plant Cell, Tissue and Organ Culture (PCTOC) 134:141–151.
- Trejgell,a., Kamıńska,M., Lısowska,K., Tretyn,A.,2018. Not Bot Horti Agrobo, 46(1):52-57. DOI:10.15835/nbha46110810).
- Valletta, A., A. R. Santamarıa, A, R., Fabrını, G., Toccı, N., Fılho, V. C., Wagner, T., Brasılı, E., Pasqua, G., 2016. Strategies for ex situ conservation of Centaurea cineraria subsp. circae (Asteraceae), an endemic plant from Lazio (Italy). Plant Biosystems, Vol. 150, No. 2, 323–332.
- White P.R. 1963. The cultivation of Animal and Plant Cells. Ronald Press, New York.
Toplam 14 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Ziraat, Veterinerlik ve Gıda Bilimleri |
| Bölüm | Original Papers |
| Yazarlar | |
| Proje Numarası | FYL-2017-5623 |
| Yayımlanma Tarihi | 30 Aralık 2020 |
| Gönderilme Tarihi | 5 Haziran 2020 |
| Kabul Tarihi | 10 Ağustos 2020 |
| Yayımlandığı Sayı | Yıl 2020 Cilt: 4 Sayı: 2 |
