BibTex RIS Kaynak Göster

Determination of Total Antioxidantand Phenolic Amount of Matured and Immature Corn Silk

Yıl 2016, Cilt: 2 Sayı: 1, 28 - 32, 25.05.2016

Öz

In this study, as an important bioactive source, potential use of corn silk extract as natural antioxidant, total antioxidant activity (AA) and phenol amount (FMM) in immature silk of dent corn and pop corn, the differences between these terms genotype and ripening conditions is aimed to be determined. In this study we have executed, total FMM was determined by the Folin-Ciocalteu method, the antioxidant activity was determined by DPPH radical reduction activity method. According to the analysis, FMM amount in the silk of dent corn (822.404 and 713.507 mg GAE g-1), was significantly higher than local pop corn silk (311.072 and 433.936 mg GAE g-1). However, FMM amount with 684.717 mg GAE g-1, the matured silk of  the local pop corn with the red kernel  has a closer FMM amount with the silk of dent corn. In the AA analysis, butylated-hydroxytoluene’s (BHT) IC50 value is determined as 225.63 mg ml-1, we used as the Standard synthetic antioxidant. In the data of the research materials, hybrid dent corn data (381.51 and 403.47 mg ml-1) is high, local pop corn data (661.29 and 687 mg ml-1) was observed to be low. Considering the situation of corn silk maturation, matured silk has higher values (P>0.005) but statistically were not significant.

Kaynakça

  • Apak R., Güçlü K., Demirata B., Özyürek M., Çelik S.E., Bektaşoğlu B., Berker KI and Özyurt D., 2007. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules, 12: 1496-1547.
  • Bonnefoy M., Drai J and Kostka T., 2002. Antioxidants to delay the process of aging: facts and perspectives. La Presse Médicale, 31(25): 1174-1184.
  • Cáceres A., Girón LM and Martinez AM., 1987. Diuretic activity of plants used for treatment of urinary ailments in Guatemala. Journal of Ethnopharmacology, 19: 233-243.
  • Cook NC and Samman S., 1996. Flavonoid schemistry, metabolism, cardio protective effects, and dietary sources. Nutritional Biochemistry, 7: 66-76.
  • Ebrahimzadeh MA., Pourmorad F and Hafe S., 2008. Antioxidant activities of Iranian corn silk. Turkish Journal of Biology, 32(1): 43-49.
  • Hashim P., 2011. Centella asiatica in food and bever age applications and its potential antioxidant and neuro protective effect. International Food Research Journal, 18(4): 1215-1222.
  • Jomova K and Valko M., 2011. Advances in metal-induced oxidative stres and human disease. Toxicology, 283: 65-87.
  • Kessler M., Ubeaud G and Jung L., 2003. Anti- and pro-oxidant activity of rutin and quercetin derivatives. Journal of Pharmacy and Pharmacology, 55(1): 131-42.
  • Koleva II., Van Beek TA., Linssen JPH de Groot A and Evstatieva LN., 2002. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochemical Analysis, 13: 8-17.
  • Köksal E., 2007. Karnabahar (Brassicaoleracea L.) peroksidaz enziminin saflaştırılması ve karakterizasyonu, antioksidan ve antiradikal aktivitesinin belirlenmesi. Doktora Tezi, Atatürk Üniversitesi Fen Bilimleri Enstitüsü, Erzurum.
  • Liu J., Wang C., Wang Z., Zhang C., Lu S and Liu J., 2011. The antioxidantand free radical scavenging activities of extract and fractions from corn silk (Zea mays L.) and related flavone glycosides. Food Chemistry, 126: 261–269.
  • Maksimović Z., Malenčić D and Kovačević N., 2005. Polyphenol contents and antioxidant activity of Maydis stigma extracts. Bioresource Technology, 96: 873-877.
  • Molyneux P., 2004. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Sonklanakarin Journal Science Technology, 262: 211-219.
  • Rop O., Ercişli S., Mlcek J., Jurikova T and Hoza I., 2014. Antioxidant and radical scavenging activities in fruits of 6 sea buckthorn (Hippophae rhamnoides L.) cultivars. Turkish Journal of Agriculture and Forestry, 38: 224-232.
  • Singleton VL., Orthofer R., Lamuela-Raventos RM., 1998. Analysis of total phenols and other oxidations unstrates and antioxidants by means of folin-ciocalteure agent. Methods in Enzymology, No 299: 152-178.
  • USDA, 2015. Foreign Agricultural Service (GAIN Reports, Grain: World Markets and Trade Reports, WASDE Reports, Production, Supply and Distrubition Database), http://www.fas.usda.gov. [Access: May 11, 2016].
  • Velazquez DVO., Xavier HS., Batista JEM and de Castro-Chaves C., 2005. Zea mays L. Extracts modify glomerular function and potassium urinary excretion in conscious rats. Journal of Phytomedicine, 12: 363-369.
  • Zhu KX., Lian CX., Guo XN., Peng W and Zhou HM., 2011. Antioxidant activities and total phenolic contents of various extracts from defatted wheat germ. Food Chemistry, 126: 1122-1126.

Olgunlaşmış ve Olgunlaşmamış Mısır Püsküllerinde Toplam Antioksidan ve Fenolik Madde Miktarlarının Belirlenmesi

Yıl 2016, Cilt: 2 Sayı: 1, 28 - 32, 25.05.2016

Öz

Yapmış olduğumuz bu çalışmada, doğal antioksidanların önemli bir biyoaktif kaynağı olarak mısır püskülü özlerinin potansiyel kullanımına yönelik, at dişi ve cin mısırında olgunlaşmış ve olgunlaşmamış püsküllerde bulunan toplam antioksidan aktivitesi (AA) ve fenolik madde miktarını (FMM) belirlemek, bu açıdan genotipler ve olgunlaşma durumları arasındaki farklılıkları belirlemek amaçlanmıştır. Yapmış olduğumuz bu çalışmada toplam FMM Folin-Ciocalteu metoduna göre, antioksidan aktivite ise DPPH radikali indirgenme aktivitesi metoduyla belirlenmiştir. Yapılan analizlerde, at dişi mısıra ait püsküllerde (822.404 ve 713.507 mg GAE g-1) bulunan FMM yerel patlak mısır püsküllerine (311.072 ve 433.936 mg GAE g-1) göre daha yüksek bulunmuştur. Ancak yerel kırmızı renkli taneye sahip olan patlak mısırın olgunlaşmış püsküllerinde bulunan FMM 684.717 mg GAE g-1 ile at dişi mısır püskülüne ait FMM’na yakın bir değer almıştır. AA analizinde ise, standart olarak kullandığımız sentetik antioksidant olan Butillenmiş-hydroxytoluen (BHT)’nin IC50 değeri 225. 63 mg ml-1 olarak belirlenmiştir. Araştırma materyallerine ait verilerde ise,  at dişi mısıra ait verilerin (381.51 ve 403.47 mg ml-1) yüksek olduğu, yerel patlak mısıra ait verilerin (661.29 ve 687 mg ml-1) ise düşük olduğu gözlemlenmiştir. Mısır püsküllerinin olgunlaşma durumları göz önüne alındığında ise olgunlaşmış püsküllere ait değerlerin daha yüksek olduğu fakat istatistiki açıdan (P>0.005) önemsiz bulunmuştur.

Kaynakça

  • Apak R., Güçlü K., Demirata B., Özyürek M., Çelik S.E., Bektaşoğlu B., Berker KI and Özyurt D., 2007. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules, 12: 1496-1547.
  • Bonnefoy M., Drai J and Kostka T., 2002. Antioxidants to delay the process of aging: facts and perspectives. La Presse Médicale, 31(25): 1174-1184.
  • Cáceres A., Girón LM and Martinez AM., 1987. Diuretic activity of plants used for treatment of urinary ailments in Guatemala. Journal of Ethnopharmacology, 19: 233-243.
  • Cook NC and Samman S., 1996. Flavonoid schemistry, metabolism, cardio protective effects, and dietary sources. Nutritional Biochemistry, 7: 66-76.
  • Ebrahimzadeh MA., Pourmorad F and Hafe S., 2008. Antioxidant activities of Iranian corn silk. Turkish Journal of Biology, 32(1): 43-49.
  • Hashim P., 2011. Centella asiatica in food and bever age applications and its potential antioxidant and neuro protective effect. International Food Research Journal, 18(4): 1215-1222.
  • Jomova K and Valko M., 2011. Advances in metal-induced oxidative stres and human disease. Toxicology, 283: 65-87.
  • Kessler M., Ubeaud G and Jung L., 2003. Anti- and pro-oxidant activity of rutin and quercetin derivatives. Journal of Pharmacy and Pharmacology, 55(1): 131-42.
  • Koleva II., Van Beek TA., Linssen JPH de Groot A and Evstatieva LN., 2002. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochemical Analysis, 13: 8-17.
  • Köksal E., 2007. Karnabahar (Brassicaoleracea L.) peroksidaz enziminin saflaştırılması ve karakterizasyonu, antioksidan ve antiradikal aktivitesinin belirlenmesi. Doktora Tezi, Atatürk Üniversitesi Fen Bilimleri Enstitüsü, Erzurum.
  • Liu J., Wang C., Wang Z., Zhang C., Lu S and Liu J., 2011. The antioxidantand free radical scavenging activities of extract and fractions from corn silk (Zea mays L.) and related flavone glycosides. Food Chemistry, 126: 261–269.
  • Maksimović Z., Malenčić D and Kovačević N., 2005. Polyphenol contents and antioxidant activity of Maydis stigma extracts. Bioresource Technology, 96: 873-877.
  • Molyneux P., 2004. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Sonklanakarin Journal Science Technology, 262: 211-219.
  • Rop O., Ercişli S., Mlcek J., Jurikova T and Hoza I., 2014. Antioxidant and radical scavenging activities in fruits of 6 sea buckthorn (Hippophae rhamnoides L.) cultivars. Turkish Journal of Agriculture and Forestry, 38: 224-232.
  • Singleton VL., Orthofer R., Lamuela-Raventos RM., 1998. Analysis of total phenols and other oxidations unstrates and antioxidants by means of folin-ciocalteure agent. Methods in Enzymology, No 299: 152-178.
  • USDA, 2015. Foreign Agricultural Service (GAIN Reports, Grain: World Markets and Trade Reports, WASDE Reports, Production, Supply and Distrubition Database), http://www.fas.usda.gov. [Access: May 11, 2016].
  • Velazquez DVO., Xavier HS., Batista JEM and de Castro-Chaves C., 2005. Zea mays L. Extracts modify glomerular function and potassium urinary excretion in conscious rats. Journal of Phytomedicine, 12: 363-369.
  • Zhu KX., Lian CX., Guo XN., Peng W and Zhou HM., 2011. Antioxidant activities and total phenolic contents of various extracts from defatted wheat germ. Food Chemistry, 126: 1122-1126.
Toplam 18 adet kaynakça vardır.

Ayrıntılar

Bölüm Tarla Bitkileri
Yazarlar

Gülay Zulkadir

Leyla İdikut

Mustafa Çölkesen

Yayımlanma Tarihi 25 Mayıs 2016
Gönderilme Tarihi 19 Ekim 2015
Yayımlandığı Sayı Yıl 2016 Cilt: 2 Sayı: 1

Kaynak Göster

APA Zulkadir, G., İdikut, L., & Çölkesen, M. (2016). Olgunlaşmış ve Olgunlaşmamış Mısır Püsküllerinde Toplam Antioksidan ve Fenolik Madde Miktarlarının Belirlenmesi. Uluslararası Tarım Ve Yaban Hayatı Bilimleri Dergisi, 2(1), 28-32.

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