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Investigation of antimicrobial and anticancer activity of extracts obtained following UV application to Althaea officinalis L. callus cultures

Yıl 2023, Cilt: 10 Sayı: 3, 425 - 436, 27.08.2023

Fethi Ahmet Özdemir Mesut Turan

https://doi.org/10.21448/ijsm.1291929

Öz

Althaea officinalis L., a plant of the Malvaceae family, is widely used in alternative medicine. The aim of this study is to cultivate the Althaea officinalis plant under in vitro conditions to create an appropriate callus regeneration protocol and investigate the antimicrobial and anticancer activities of methanol and ethyl acetate extracts of calli after UV-C application. Leaf, petiole, and root parts of A. officinalis plants germinated in a sterile environment were used as explant sources. Explants were cultured on MS medium containing different concentrations of 2,4-D (1, 2 mg/l) and BAP (0.25, 0.50, 0.75 mg/l). The most effective (100%) callus growth and callus weight (516.24±0.48 mg) was observed on petiole explants using MS medium containing 1 mg/l 2,4-D + 0.25 mg/l BAP. Calli obtained from leaf and petiole explants were exposed to UV-C treatment. Extractions of calli were carried out using methanol and ethyl acetate solutions. 1 mg/ml, 5 mg/ml, and 10 mg/ml solutions of methanol and ethyl acetate extracts were prepared and their antimicrobial activity on bacteria was investigated using the disc diffusion method for 7 different gram-positive and 9 different gram-negative bacteria. None of the three extract concentrations used had any antimicrobial activities. The anticancer activities of the extracts on SH-SY5Y human neuroblastoma cells were studied using the WST-1 viability kit. 1000, 500, 250, 125, and 62.5 µg/ml concentrations of ethyl acetate extracts of leaf and petiole calli had anticancer activity.

Anahtar Kelimeler

Althaea officinalis L. Callus Extraction Antimicrobial activity Anticancer activity

Kaynakça

  • Ahmad, I., Mehmood, Z., & Mohammad, F. (1998). Screening of some Indian medicinal plants for their antimicrobial properties. Journal of Ethnopharmacol, 62, 183–193.
  • Alshaya, Z.E.H., Kadhim, E.J., & Sahib, H.B. (2019). Antiproliferative activities of Althaea ludwigii L. extract on Michigan Cancer Foundation-7 breast cancer cell line, Journal of Applied Biology & Biotechnology, 7(03), 9-11.
  • Al-Snafi, A.E. (2013). The pharmaceutical importance of Althaea officinalis and A Review. International Journal of PharmTech Research, 5(3), 1387-1385.
  • Altan, A. (2001). Özel gıdalar teknolojisi, 3. Baskı. [Specialty foods technology, 3rd Edition]. Çukurova Üniversitesi Ziraat Fakültesi Genel Yayın No: 178 Adana.
  • Baytop, T. (1984). Türkiye’de bitkiler ile tedavi [Treatment with herbs in Türkiye]. İstanbul Üniversitesi Yayın no: 3637, Eczacılık Fakültesi, İstabul, 240-376.
  • Bodeker, G. (2002). Tıbbi Bitkiler: Sürdürülebilirlik ve Güvenliğe Doğru; IDRC Şifalı Bitkiler Global Network WOCMAP III için Sponsorlu Tartışma Belgesi. [Medicinal Plants: Towards sustainability and safety; sponsored discussion paper for IDRC herbs global network WOCMAP III]. Tayland: Chiang Mai.
  • Bonjar, S.B. (2004). Evaluation of antibacterial properties of some medicinal plants used. Iran Journal of Ethnopharmacol, 94, 301-305.
  • Changizi, A.S., Yarmohammadi, P., Hosseini, N., Salehi, I., & Ramazani, M. (2015). The effect of Althaea officinalis L. root alcoholic extract on blood sugar level and lipid profiles of streptozotocin induced-diabetic rats. Iranian Journal of Endocrinology and Metabolism, 17(3), 238–250.
  • Davis, P.H. (1984). Flora of turkey and east aegean islands. University Press. Edinburgh, s. 8.
  • Ding, Z., Dai, Y., Hao, H., Pan, R., Yao, X., & Wang, Z. (2008). Anti-inflammatory effects of scopoletin and underlying mechanisms. Pharmaceutical Biology, 46(12), 854–860.
  • Diplock, A. (1998). Healty lifestyles nutrition and physical aktivity:antioxidant nutritiens. Europe Consice Monograph Series Belgium, p.59.
  • Elmastas, M., Ozturk, L., Gokce, I., Erenler, R., & Aboul-Enein, H.Y. (2004). Detremination of antioxidant activity of marshmallow flower (Althaea officinalis). Anal. Lett, 37, 1859-1869.
  • Gutam, S.S.N., Kumar, S., & Chauhan, R. (2015). Antimicrobial efficacy of Althaea officinalis linn. seed extracts and essential oil against respiratory tract pathogens. Journal of Applied Pharmaceutical Science, 5(9), 115-119.
  • Lin, J., Opoku, A.R., Geheeb-Keller, M., Hutchings, A.D., Terblanche, S.E., Jager, A.K., & Van Staden, J. (1999). Preliminary screening of some traditional zulu medicinal plants for antiinflammatory and anti-microbial activities. Journal of Ethnopharmacol, 68, 267–274.
  • May, G., & Willuhn, G. (1985). Antiviral activity of aqueous extracts from medicinal plants in tissue cultures. Arzneim-Forsch, 28(1), 1-7.
  • Mujib, A., Pipal, T., Ali, M., Tonk, D., Zafar, N., & Gulzar, B. (2017). In vitro propagation of Althaea officinalis: the role of plant growth regulators in morphogenesis. Journal of Biotechnology, Computational Biology and Bionanotechnology, 98(3), 167-173.
  • Naz, R., & Anis, M. (2012). Acceleration of adventitious shoots by interaction between exogenous hormone and adenine sulphate in Althaea officinalis L. Applied Biochemistry Biotechnol, 168, 1239–1255.
  • Naz, R., Anis, M., & Aref, I.M. (2015). Management of cytokinin–auxin interactions for in vitro shoot proliferation of Althaea officinalis L. a valuable medicinal plant. Rend. Fis. Acc. Lincei, 26, 323–334.
  • Park, H.W., Jang, K.H., Lee, K.S., & Lee, D.J. (2010). Antioxidant, anti-inflammatory and anticancer activities in Abelomoschus esculentus. and Althaea officinalis. College of Bio-resource Sciences, Dankook University, 2(83), 447-448.
  • Qaralleh, H., Al-Limoun, M.O., Khlaifat, A., Khleifat, K.M., Al-Tawarah, N., Alsharafa, K.Y., & Harirah, H.A.A. (2020). Antibacterial and antibiofilm activities of a traditional herbal formula against respiratory infection causing bacteria. Tropical Journal of Natural Product Research, 4(9), 527-534.
  • Rezai, M., Dadgar, Z., Zadeh, A.N., Namin, S.A.M., & Pakzad, I. (2015). Elham davodian, evaluation of the antibacterial activity of the Althaea Officinalis L. leaf extract and its wound healing potency in the rat model of excision wound creation. Avicenna Journal of Phytomedicine, 5(2), 105-112.
  • Twaij, B.M., & Alwan, A.H. (2018). Anti-Fungal activity of Althaea officinalis L. tissue culture extracts. Plant Archives, 18(2), 2053-2057.
  • Zhang, Y., Kong, F., Zhang, L., Li, C., & Zhang, R. (2016). Modulatory effect of Althaea officinalis L. root extract on cisplatin-induced cytotoxicity and cell proliferation in A549 human lung cancer cell line. Tropical Journal of Pharmaceutical Research, (12), 2647-2652.

Investigation of antimicrobial and anticancer activity of extracts obtained following UV application to Althaea officinalis L. callus cultures

Yıl 2023, Cilt: 10 Sayı: 3, 425 - 436, 27.08.2023

Fethi Ahmet Özdemir Mesut Turan

https://doi.org/10.21448/ijsm.1291929

Öz

Althaea officinalis L., a plant of the Malvaceae family, is widely used in alternative medicine. The aim of this study is to cultivate the Althaea officinalis plant under in vitro conditions to create an appropriate callus regeneration protocol and investigate the antimicrobial and anticancer activities of methanol and ethyl acetate extracts of calli after UV-C application. Leaf, petiole, and root parts of A. officinalis plants germinated in a sterile environment were used as explant sources. Explants were cultured on MS medium containing different concentrations of 2,4-D (1, 2 mg/l) and BAP (0.25, 0.50, 0.75 mg/l). The most effective (100%) callus growth and callus weight (516.24±0.48 mg) was observed on petiole explants using MS medium containing 1 mg/l 2,4-D + 0.25 mg/l BAP. Calli obtained from leaf and petiole explants were exposed to UV-C treatment. Extractions of calli were carried out using methanol and ethyl acetate solutions. 1 mg/ml, 5 mg/ml, and 10 mg/ml solutions of methanol and ethyl acetate extracts were prepared and their antimicrobial activity on bacteria was investigated using the disc diffusion method for 7 different gram-positive and 9 different gram-negative bacteria. None of the three extract concentrations used had any antimicrobial activities. The anticancer activities of the extracts on SH-SY5Y human neuroblastoma cells were studied using the WST-1 viability kit. 1000, 500, 250, 125, and 62.5 µg/ml concentrations of ethyl acetate extracts of leaf and petiole calli had anticancer activity.

Anahtar Kelimeler

Althaea officinalis L. Callus Extraction Antimicrobial activity Anticancer activity

Kaynakça

  • Ahmad, I., Mehmood, Z., & Mohammad, F. (1998). Screening of some Indian medicinal plants for their antimicrobial properties. Journal of Ethnopharmacol, 62, 183–193.
  • Alshaya, Z.E.H., Kadhim, E.J., & Sahib, H.B. (2019). Antiproliferative activities of Althaea ludwigii L. extract on Michigan Cancer Foundation-7 breast cancer cell line, Journal of Applied Biology & Biotechnology, 7(03), 9-11.
  • Al-Snafi, A.E. (2013). The pharmaceutical importance of Althaea officinalis and A Review. International Journal of PharmTech Research, 5(3), 1387-1385.
  • Altan, A. (2001). Özel gıdalar teknolojisi, 3. Baskı. [Specialty foods technology, 3rd Edition]. Çukurova Üniversitesi Ziraat Fakültesi Genel Yayın No: 178 Adana.
  • Baytop, T. (1984). Türkiye’de bitkiler ile tedavi [Treatment with herbs in Türkiye]. İstanbul Üniversitesi Yayın no: 3637, Eczacılık Fakültesi, İstabul, 240-376.
  • Bodeker, G. (2002). Tıbbi Bitkiler: Sürdürülebilirlik ve Güvenliğe Doğru; IDRC Şifalı Bitkiler Global Network WOCMAP III için Sponsorlu Tartışma Belgesi. [Medicinal Plants: Towards sustainability and safety; sponsored discussion paper for IDRC herbs global network WOCMAP III]. Tayland: Chiang Mai.
  • Bonjar, S.B. (2004). Evaluation of antibacterial properties of some medicinal plants used. Iran Journal of Ethnopharmacol, 94, 301-305.
  • Changizi, A.S., Yarmohammadi, P., Hosseini, N., Salehi, I., & Ramazani, M. (2015). The effect of Althaea officinalis L. root alcoholic extract on blood sugar level and lipid profiles of streptozotocin induced-diabetic rats. Iranian Journal of Endocrinology and Metabolism, 17(3), 238–250.
  • Davis, P.H. (1984). Flora of turkey and east aegean islands. University Press. Edinburgh, s. 8.
  • Ding, Z., Dai, Y., Hao, H., Pan, R., Yao, X., & Wang, Z. (2008). Anti-inflammatory effects of scopoletin and underlying mechanisms. Pharmaceutical Biology, 46(12), 854–860.
  • Diplock, A. (1998). Healty lifestyles nutrition and physical aktivity:antioxidant nutritiens. Europe Consice Monograph Series Belgium, p.59.
  • Elmastas, M., Ozturk, L., Gokce, I., Erenler, R., & Aboul-Enein, H.Y. (2004). Detremination of antioxidant activity of marshmallow flower (Althaea officinalis). Anal. Lett, 37, 1859-1869.
  • Gutam, S.S.N., Kumar, S., & Chauhan, R. (2015). Antimicrobial efficacy of Althaea officinalis linn. seed extracts and essential oil against respiratory tract pathogens. Journal of Applied Pharmaceutical Science, 5(9), 115-119.
  • Lin, J., Opoku, A.R., Geheeb-Keller, M., Hutchings, A.D., Terblanche, S.E., Jager, A.K., & Van Staden, J. (1999). Preliminary screening of some traditional zulu medicinal plants for antiinflammatory and anti-microbial activities. Journal of Ethnopharmacol, 68, 267–274.
  • May, G., & Willuhn, G. (1985). Antiviral activity of aqueous extracts from medicinal plants in tissue cultures. Arzneim-Forsch, 28(1), 1-7.
  • Mujib, A., Pipal, T., Ali, M., Tonk, D., Zafar, N., & Gulzar, B. (2017). In vitro propagation of Althaea officinalis: the role of plant growth regulators in morphogenesis. Journal of Biotechnology, Computational Biology and Bionanotechnology, 98(3), 167-173.
  • Naz, R., & Anis, M. (2012). Acceleration of adventitious shoots by interaction between exogenous hormone and adenine sulphate in Althaea officinalis L. Applied Biochemistry Biotechnol, 168, 1239–1255.
  • Naz, R., Anis, M., & Aref, I.M. (2015). Management of cytokinin–auxin interactions for in vitro shoot proliferation of Althaea officinalis L. a valuable medicinal plant. Rend. Fis. Acc. Lincei, 26, 323–334.
  • Park, H.W., Jang, K.H., Lee, K.S., & Lee, D.J. (2010). Antioxidant, anti-inflammatory and anticancer activities in Abelomoschus esculentus. and Althaea officinalis. College of Bio-resource Sciences, Dankook University, 2(83), 447-448.
  • Qaralleh, H., Al-Limoun, M.O., Khlaifat, A., Khleifat, K.M., Al-Tawarah, N., Alsharafa, K.Y., & Harirah, H.A.A. (2020). Antibacterial and antibiofilm activities of a traditional herbal formula against respiratory infection causing bacteria. Tropical Journal of Natural Product Research, 4(9), 527-534.
  • Rezai, M., Dadgar, Z., Zadeh, A.N., Namin, S.A.M., & Pakzad, I. (2015). Elham davodian, evaluation of the antibacterial activity of the Althaea Officinalis L. leaf extract and its wound healing potency in the rat model of excision wound creation. Avicenna Journal of Phytomedicine, 5(2), 105-112.
  • Twaij, B.M., & Alwan, A.H. (2018). Anti-Fungal activity of Althaea officinalis L. tissue culture extracts. Plant Archives, 18(2), 2053-2057.
  • Zhang, Y., Kong, F., Zhang, L., Li, C., & Zhang, R. (2016). Modulatory effect of Althaea officinalis L. root extract on cisplatin-induced cytotoxicity and cell proliferation in A549 human lung cancer cell line. Tropical Journal of Pharmaceutical Research, (12), 2647-2652.
Toplam 23 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Bitki Biyokimyası, Yapısal Biyoloji , Farmakognozi
Bölüm Makaleler
Yazarlar

Fethi Ahmet Özdemir 0000-0001-7215-9692

Mesut Turan Bu kişi benim 0000-0002-4980-7337

Erken Görünüm Tarihi 31 Temmuz 2023
Yayımlanma Tarihi 27 Ağustos 2023
Gönderilme Tarihi 3 Mayıs 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 10 Sayı: 3

Kaynak Göster

APA Özdemir, F. A., & Turan, M. (2023). Investigation of antimicrobial and anticancer activity of extracts obtained following UV application to Althaea officinalis L. callus cultures. International Journal of Secondary Metabolite, 10(3), 425-436. https://doi.org/10.21448/ijsm.1291929
International Journal of Secondary Metabolite
e-ISSN: 2148-6905