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Microsatellite analysis in some watermelon (Citrullus lanatus) genotypes

Yıl 2022, Cilt: 6 Sayı: 1, 58 - 64, 15.03.2022

Solmaz Kamaşak Ömer Faruk Coşkun Akife Dalda Şekerci Osman Gülşen

https://doi.org/10.31015/jaefs.2022.1.9
Cited By: 2

Öz

Conservation of genetic resources is essential for the continuation of future crop production. Watermelon (Citrul-lus lanatus), a member of Cucurbitaceae, is widely distributed in tropical and subtropical regions. The aim of this study was to reveal the genetic relationships with the help of microsatellite markers in a watermelon collection free of unnecessary repetitions, and to determine the success of SSR (Simple Sequence Repeats) primers developed in cucurbits. In this study, 96 watermelon genotypes with good agronomic characteristics were used among the geno types collected from different regions of Turkey and purified up to the S4-S6 (self-pollination) stage. In the study, 33 SSR primer pairs were used to determine the genetic relationship between watermelon genotypes. In the study, a total of 67 bands were obtained with SSR primers. As a result of UPGMA (Unweighted Pair Group Method Using Arithmetic Averages) analysis, genotypes showed similarity at the level of 0.84-1.00. The number of alleles detected per primer varied between 1 and 6. In terms of the total number of alleles obtained, CMCT44 (5 units) and Cgb4767 (6 units) loci produced the most alleles. Primers with high polymorphism rate and allele excess were determined, and the possibilities for use in genetic stability analyses, variety differentiation and other genetic analyses were determined.

Anahtar Kelimeler

Citrullus lanatus watermelon SSR microsatellite genetic characterization

Kaynakça

  • Anonymous, (2019). “FAOSTAT, Watermelon production statistics”, http://www.fao.org/statistics/en/, July 2021
  • Aslan, N., Coskun, O.F., Dalda-Sekerci, A., & Gulsen, O. (2021). Moleküler markörler kullanarak çerezlik kabaklarda (Cucurbita pepo L.) saflık düzeylerinin tahmin edilmesi. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 26 (3), 759-769. https://doi.org/10.37908/mkutbd.995779
  • Collins, J.K., Perkins-Veazie, P., & Roberts, W. (2006). Lycopene: from plants to humans. HortScience, 41(5), 1135–1144. https://doi.org/10.21273/HORTSCI.41.5.1135
  • Coskun, O.F., Gulsen, O., Dalda-Sekerci, A., Yetisir, H., & Pınar, H. (2017). Bazı çerezlik kabak hatlarında SSR markır analizi. Akademik Ziraat Dergisi, 6, 151-156.
  • Danin-Poleg, Y., Reis, N., Tzuri, G., & Katzir, N. (2001). Development and characterization of microsatellite markers in Cucumis. Theoretical and Applied Genetics, 102(1), 61–72. https://doi.org/10.1007/s001220051618
  • Düzyaman, E. (2013). Karpuz Hakkında Herşey, Hasad Yayıncılık, Ankara.
  • Gama, R.N., Santos, C.A.F., Dias, R.D.C.S., & Souza, F.F. (2013). Molecular characterization of watermelon cultivars using microsatellite markers. Horticultura Brasileira, 31(4), 522–527. https://doi.org/10.1590/S0102-05362013000400003
  • Guerra ‐ Sanz, J.M. (2002). Citrullus simple sequence repeats markers from sequence databases. Molecular Ecology Notes, 2 (3), 223–225. https://doi.org/10.1046/j.1471-8286.2002.00200.x
  • Hwang, J.H., Ahn, S.G., Oh, J.Y., Choi, Y.W., Kang, J.S., & Park, Y.H. (2011). Functional characterization of watermelon (Citrullus lanatus L.) EST–SSR by gel electrophoresis and high resolution melting analysis. Scientia Horticulturae, 130(4), 715–724. https://doi.org/10.1016/j.scienta.2011.08.014
  • Karaman, K., Dalda-Şekerci, A., Yetişir, H., Gülşen, O., & Coskun, O.F. (2018). Molecular, morphological and biochemical characterization of some Turkish bitter melon (Momordica charantia L.) genotypes. Industrial Crops and Products, 123, 93–99. https://doi.org/10.1016/j.indcrop.2018.06.036
  • Kırac, H., Dalda-Sekerci, A., Coskun, O.F., & Gulsen, O. (2022). Morphological and molecular characterization of garlic (Allium sativum L.) genotypes sampled from Turkey. Genetic Resources and Crop Evolution, 1-9. https://doi.org/10.1007/s10722-022-01343-4
  • Kong, Q., Chen, J., Liu, Y., Ma, Y., Liu, P., Wu, S., & Bie, Z. (2014). Genetic diversity of Cucurbita rootstock germplasm as assessed using simple sequence repeat markers. Scientia Horticulturae, 175, 150–155. https://doi.org/10.1016/j.scienta.2014.06.009
  • Kwon, Y.S. (2013). Use of EST-SSR markers for genetic characterisation of commercial watermelon varieties and hybrid seed purity testing. Seed Sci. & Technol., 41, 245–256. https://doi.org/10.15258/sst.2013.41.2.07
  • Lombard, V., Dubreuil, P., Dillmann, C., Baril, C. (2001). Genetic distance estimators based on molecular data for plant registration and protection: a review. Acta Hort., 546, 55–63. https://doi.org/10.17660/ActaHortic.2001.546.2
  • Lu, X., Adedze, Y.M.N., Chofong, G.N., Gandeka, M., Deng, Z., Teng, L., & Li, W. (2018). Identification of high-efficiency SSR markers for assessing watermelon genetic purity. Journal of Genetics, 97(5), 1295–1306. https://doi.org/10.1007/s12041-018-1027-4
  • Morilipınar, E.O., Dalda-Sekerci, A., Coskun, O.F., & Gulsen, O. (2021). Genetic analysis of local pumpkin populations. International Journal of Agricultural and Natural Sciences, 14 (3), 264-272.
  • Nantoume, A.D., Andersen, S.B., & Jensen, B.D. (2013). Genetic differentiation of watermelon landrace types in Mali revealed by microsatellite (SSR) markers. Genetic Resources and Crop Evolution, 60(7), 2129–2141. https://doi.org/10.1007/s10722-013-9980-5
  • Navot, N., & Zamir, D. (1987). Isozyme and seed protein phylogeny of genus Citrullus (Cucurbitaceae). Plant Systematics and Evolution, 156, 61–67. https://doi.org/10.1007/s10722-013-9980-5
  • Powel, W., Morgante, M., Andre, C., Hanafey, M., Vogel, J., Tingey, S., & Rafalski, A. (1996). The Comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding, 2 (3), 225–238. https://doi.org/10.1007/BF00564200
  • Röder, M.S., Plaschke, J., König, S.U., Börner, A., Sorrells, M.E., Tanksley, S.D., & Ganal, M.W. (1995). Abundance, variability and chromosomal location of microsatellites in wheat. Molecular and General Genetics MGG, 246(3), 327–333. https://doi.org/10.1007/BF00288605
  • Sarı, N., Solmaz, I., Yetişir, & H., Ünlü, H. (2007). Watermelon genetic resources in Turkey and their characteristics. Acta Hortic., 731, 433–438.
  • Solmaz, I. (2010). Bazı karpuz genotiplerinin SSR ve SRAP markörleri ile karakterizasyonu ve fusarium solgunluğu (Fusarium oxysporum f. sp. niveum)’na dayanımlarının klasik ve moleküler yöntemlerle araştırılması. Doktora Tezi, ÇÜ Fen Bilimleri Enstitüsü, Adana.
  • Solmaz, I., Sarı, N., Aka-Kaçar, Y., & Yalçın-Mendi, N.Y. (2010). The genetic characterization of Turkish watermelon (Citrullus lanatus) accessions using RAPD markers. Genetic Resources and Crop Evolution, 57(5), 763–771. https://doi.org/10.1007/s10722-009-9515-2
  • Tlili, I., Hdider, C., Ilahy, R., & Jebari, H. (2010). Phytochemical composition and antioxidant activity of selected watermelon varieties grown in Tunisia. The African Journal of Plant Science and Biotechnology, 68–71.
  • Tzitzikas, E.M., Monforte, A.J., Fatihi, A., Kypriotakis, A., Iacovides, T.A., Ioannides, I.M., & Kalaitzis, P. (2009). Genetic diversity and population structure of traditional Greek and Cypriot melon134 cultigens (Cucumis melo L.) based on simple sequence repeat variability. Hort Science, 44 (7), 1820–1824. https://doi.org/10.21273/HORTSCI.44.7.1820
  • Uzun, A., Cil, A., Yaman, M., & Coskun, O.F. (2020). Genetic diversity and some fruit characteristics of quince genotypes collected from Kayseri region. Turkish Journal of Agriculture - Food Science and Technology, 8(2), 318–323. https://doi.org/10.24925/turjaf.v8i2.318-323.3012
  • Zhang, H., Wang, H., Guo, S., Ren, Y., Gong, G., Weng, Y., & Xu, Y. (2012). Identification and validation of a core set of microsatellite markers for genetic diversity analysis in watermelon, Citrullus lanatus Thunb. Matsum. & Nakai. Euphytica, 186(2), 329–342. https://doi.org/10.1007/s10681-011-0574-z

Yıl 2022, Cilt: 6 Sayı: 1, 58 - 64, 15.03.2022

Solmaz Kamaşak Ömer Faruk Coşkun Akife Dalda Şekerci Osman Gülşen

https://doi.org/10.31015/jaefs.2022.1.9
Cited By: 2

Öz

Kaynakça

  • Anonymous, (2019). “FAOSTAT, Watermelon production statistics”, http://www.fao.org/statistics/en/, July 2021
  • Aslan, N., Coskun, O.F., Dalda-Sekerci, A., & Gulsen, O. (2021). Moleküler markörler kullanarak çerezlik kabaklarda (Cucurbita pepo L.) saflık düzeylerinin tahmin edilmesi. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 26 (3), 759-769. https://doi.org/10.37908/mkutbd.995779
  • Collins, J.K., Perkins-Veazie, P., & Roberts, W. (2006). Lycopene: from plants to humans. HortScience, 41(5), 1135–1144. https://doi.org/10.21273/HORTSCI.41.5.1135
  • Coskun, O.F., Gulsen, O., Dalda-Sekerci, A., Yetisir, H., & Pınar, H. (2017). Bazı çerezlik kabak hatlarında SSR markır analizi. Akademik Ziraat Dergisi, 6, 151-156.
  • Danin-Poleg, Y., Reis, N., Tzuri, G., & Katzir, N. (2001). Development and characterization of microsatellite markers in Cucumis. Theoretical and Applied Genetics, 102(1), 61–72. https://doi.org/10.1007/s001220051618
  • Düzyaman, E. (2013). Karpuz Hakkında Herşey, Hasad Yayıncılık, Ankara.
  • Gama, R.N., Santos, C.A.F., Dias, R.D.C.S., & Souza, F.F. (2013). Molecular characterization of watermelon cultivars using microsatellite markers. Horticultura Brasileira, 31(4), 522–527. https://doi.org/10.1590/S0102-05362013000400003
  • Guerra ‐ Sanz, J.M. (2002). Citrullus simple sequence repeats markers from sequence databases. Molecular Ecology Notes, 2 (3), 223–225. https://doi.org/10.1046/j.1471-8286.2002.00200.x
  • Hwang, J.H., Ahn, S.G., Oh, J.Y., Choi, Y.W., Kang, J.S., & Park, Y.H. (2011). Functional characterization of watermelon (Citrullus lanatus L.) EST–SSR by gel electrophoresis and high resolution melting analysis. Scientia Horticulturae, 130(4), 715–724. https://doi.org/10.1016/j.scienta.2011.08.014
  • Karaman, K., Dalda-Şekerci, A., Yetişir, H., Gülşen, O., & Coskun, O.F. (2018). Molecular, morphological and biochemical characterization of some Turkish bitter melon (Momordica charantia L.) genotypes. Industrial Crops and Products, 123, 93–99. https://doi.org/10.1016/j.indcrop.2018.06.036
  • Kırac, H., Dalda-Sekerci, A., Coskun, O.F., & Gulsen, O. (2022). Morphological and molecular characterization of garlic (Allium sativum L.) genotypes sampled from Turkey. Genetic Resources and Crop Evolution, 1-9. https://doi.org/10.1007/s10722-022-01343-4
  • Kong, Q., Chen, J., Liu, Y., Ma, Y., Liu, P., Wu, S., & Bie, Z. (2014). Genetic diversity of Cucurbita rootstock germplasm as assessed using simple sequence repeat markers. Scientia Horticulturae, 175, 150–155. https://doi.org/10.1016/j.scienta.2014.06.009
  • Kwon, Y.S. (2013). Use of EST-SSR markers for genetic characterisation of commercial watermelon varieties and hybrid seed purity testing. Seed Sci. & Technol., 41, 245–256. https://doi.org/10.15258/sst.2013.41.2.07
  • Lombard, V., Dubreuil, P., Dillmann, C., Baril, C. (2001). Genetic distance estimators based on molecular data for plant registration and protection: a review. Acta Hort., 546, 55–63. https://doi.org/10.17660/ActaHortic.2001.546.2
  • Lu, X., Adedze, Y.M.N., Chofong, G.N., Gandeka, M., Deng, Z., Teng, L., & Li, W. (2018). Identification of high-efficiency SSR markers for assessing watermelon genetic purity. Journal of Genetics, 97(5), 1295–1306. https://doi.org/10.1007/s12041-018-1027-4
  • Morilipınar, E.O., Dalda-Sekerci, A., Coskun, O.F., & Gulsen, O. (2021). Genetic analysis of local pumpkin populations. International Journal of Agricultural and Natural Sciences, 14 (3), 264-272.
  • Nantoume, A.D., Andersen, S.B., & Jensen, B.D. (2013). Genetic differentiation of watermelon landrace types in Mali revealed by microsatellite (SSR) markers. Genetic Resources and Crop Evolution, 60(7), 2129–2141. https://doi.org/10.1007/s10722-013-9980-5
  • Navot, N., & Zamir, D. (1987). Isozyme and seed protein phylogeny of genus Citrullus (Cucurbitaceae). Plant Systematics and Evolution, 156, 61–67. https://doi.org/10.1007/s10722-013-9980-5
  • Powel, W., Morgante, M., Andre, C., Hanafey, M., Vogel, J., Tingey, S., & Rafalski, A. (1996). The Comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding, 2 (3), 225–238. https://doi.org/10.1007/BF00564200
  • Röder, M.S., Plaschke, J., König, S.U., Börner, A., Sorrells, M.E., Tanksley, S.D., & Ganal, M.W. (1995). Abundance, variability and chromosomal location of microsatellites in wheat. Molecular and General Genetics MGG, 246(3), 327–333. https://doi.org/10.1007/BF00288605
  • Sarı, N., Solmaz, I., Yetişir, & H., Ünlü, H. (2007). Watermelon genetic resources in Turkey and their characteristics. Acta Hortic., 731, 433–438.
  • Solmaz, I. (2010). Bazı karpuz genotiplerinin SSR ve SRAP markörleri ile karakterizasyonu ve fusarium solgunluğu (Fusarium oxysporum f. sp. niveum)’na dayanımlarının klasik ve moleküler yöntemlerle araştırılması. Doktora Tezi, ÇÜ Fen Bilimleri Enstitüsü, Adana.
  • Solmaz, I., Sarı, N., Aka-Kaçar, Y., & Yalçın-Mendi, N.Y. (2010). The genetic characterization of Turkish watermelon (Citrullus lanatus) accessions using RAPD markers. Genetic Resources and Crop Evolution, 57(5), 763–771. https://doi.org/10.1007/s10722-009-9515-2
  • Tlili, I., Hdider, C., Ilahy, R., & Jebari, H. (2010). Phytochemical composition and antioxidant activity of selected watermelon varieties grown in Tunisia. The African Journal of Plant Science and Biotechnology, 68–71.
  • Tzitzikas, E.M., Monforte, A.J., Fatihi, A., Kypriotakis, A., Iacovides, T.A., Ioannides, I.M., & Kalaitzis, P. (2009). Genetic diversity and population structure of traditional Greek and Cypriot melon134 cultigens (Cucumis melo L.) based on simple sequence repeat variability. Hort Science, 44 (7), 1820–1824. https://doi.org/10.21273/HORTSCI.44.7.1820
  • Uzun, A., Cil, A., Yaman, M., & Coskun, O.F. (2020). Genetic diversity and some fruit characteristics of quince genotypes collected from Kayseri region. Turkish Journal of Agriculture - Food Science and Technology, 8(2), 318–323. https://doi.org/10.24925/turjaf.v8i2.318-323.3012
  • Zhang, H., Wang, H., Guo, S., Ren, Y., Gong, G., Weng, Y., & Xu, Y. (2012). Identification and validation of a core set of microsatellite markers for genetic diversity analysis in watermelon, Citrullus lanatus Thunb. Matsum. & Nakai. Euphytica, 186(2), 329–342. https://doi.org/10.1007/s10681-011-0574-z
Toplam 27 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Bahçe Bitkileri Yetiştirme ve Islahı
Bölüm Makaleler
Yazarlar

Solmaz Kamaşak 0000-0002-4404-9178

Ömer Faruk Coşkun 0000-0001-5398-5737

Akife Dalda Şekerci 0000-0001-8554-6501

Osman Gülşen 0000-0003-1894-9997

Yayımlanma Tarihi 15 Mart 2022
Gönderilme Tarihi 15 Aralık 2021
Kabul Tarihi 9 Mart 2022
Yayımlandığı Sayı Yıl 2022 Cilt: 6 Sayı: 1

Kaynak Göster

APA Kamaşak, S., Coşkun, Ö. F., Dalda Şekerci, A., Gülşen, O. (2022). Microsatellite analysis in some watermelon (Citrullus lanatus) genotypes. International Journal of Agriculture Environment and Food Sciences, 6(1), 58-64. https://doi.org/10.31015/jaefs.2022.1.9
AMA Kamaşak S, Coşkun ÖF, Dalda Şekerci A, Gülşen O. Microsatellite analysis in some watermelon (Citrullus lanatus) genotypes. int. j. agric. environ. food sci. Mart 2022;6(1):58-64. doi:10.31015/jaefs.2022.1.9
Chicago Kamaşak, Solmaz, Ömer Faruk Coşkun, Akife Dalda Şekerci, ve Osman Gülşen. “Microsatellite Analysis in Some Watermelon (Citrullus Lanatus) Genotypes”. International Journal of Agriculture Environment and Food Sciences 6, sy. 1 (Mart 2022): 58-64. https://doi.org/10.31015/jaefs.2022.1.9.
EndNote Kamaşak S, Coşkun ÖF, Dalda Şekerci A, Gülşen O (01 Mart 2022) Microsatellite analysis in some watermelon (Citrullus lanatus) genotypes. International Journal of Agriculture Environment and Food Sciences 6 1 58–64.
IEEE S. Kamaşak, Ö. F. Coşkun, A. Dalda Şekerci, ve O. Gülşen, “Microsatellite analysis in some watermelon (Citrullus lanatus) genotypes”, int. j. agric. environ. food sci., c. 6, sy. 1, ss. 58–64, 2022, doi: 10.31015/jaefs.2022.1.9.
ISNAD Kamaşak, Solmaz vd. “Microsatellite Analysis in Some Watermelon (Citrullus Lanatus) Genotypes”. International Journal of Agriculture Environment and Food Sciences 6/1 (Mart 2022), 58-64. https://doi.org/10.31015/jaefs.2022.1.9.
JAMA Kamaşak S, Coşkun ÖF, Dalda Şekerci A, Gülşen O. Microsatellite analysis in some watermelon (Citrullus lanatus) genotypes. int. j. agric. environ. food sci. 2022;6:58–64.
MLA Kamaşak, Solmaz vd. “Microsatellite Analysis in Some Watermelon (Citrullus Lanatus) Genotypes”. International Journal of Agriculture Environment and Food Sciences, c. 6, sy. 1, 2022, ss. 58-64, doi:10.31015/jaefs.2022.1.9.
Vancouver Kamaşak S, Coşkun ÖF, Dalda Şekerci A, Gülşen O. Microsatellite analysis in some watermelon (Citrullus lanatus) genotypes. int. j. agric. environ. food sci. 2022;6(1):58-64.

Cited By

Determination of genetic diversity of edible-seeded watermelon genotypes using SRAP markers
Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi
https://doi.org/10.37908/mkutbd.1359989
Determination of Genetic Diversity in Some Pumpkin Genotypes Using SSR Marker Technique
Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi
https://doi.org/10.18185/erzifbed.1113553
 Kapak Resmi İndir

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