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Effects of different combinations of growth regulators on the in vitro growth parameters of Poncirus trifoliata L. (Raf.)

Yıl 2022, Cilt: 6 Sayı: 4, 598 - 604, 30.12.2022

Elif Subasi Sevinc Bekir Erol Ak Ibrahim Halil Hatipoglu Heydem Ekinci

https://doi.org/10.31015/jaefs.2022.4.13

Öz

In this study, an in vitro propagation protocol of Poncirus trifoliata L. (Raf.) rootstock, which is one of the main rootstocks used in citrus, was created. Monocular node parts of the plant, which is the source of explant, were cultured in MS nutrient media containing BAP at dissmilar concentrations (0.5-1.0-2.0 mg/l). Afterward, it was observed that shoot appearance and tillering were achieved in the node explants cultured in MS nutrient medium comprising 1.0 mg/l BAP. The shoots were removed to a new culture in MS nutrient media containing NAA and IBA for rooting. While 30% rooting was achieved in the rooting medium, 62% of the rooted plantlets were acclimated to external conditions. 2 mg/l BAP and 2 mg/l silver nitrate (AgNO3) doses were supplied to the MS medium. The results of this study were obtained and a compound was found to the yellowing and leaf fall problem. As a result, in vitro propagation of Poncirus trifoliata, which is among the important citrus rootstocks, was tried, and its rooting ability was investigated in different nutrient media concentrations after shoots were obtained from different explants.

Anahtar Kelimeler

Poncirus trifoliata L. in vitro silver nitrate ornamental plant propagation tissue culture

Destekleyen Kurum

Harran University Scientific Research Projects Coordination Unit (HUBAK)

Proje Numarası

21096

Kaynakça

  • Ainsley, P.J., Collins, G.G., Sedgley, M. (2001a). In Vitro Rooting of Almond (Prunus dulcis Mill.). In vitro Cellular and Development Biology Plant, 37:778-785.
  • Ainsley, P.J., Hammerschlag, F.A., Bertozzi, T., Collins, G.G., Sedgley, M. (2001b). Regeneration of Almond from Immature Seed Cotyledons. Plant Cell, Tissue and Organ Culture, 67: 221-226.
  • Ali, S., Mirza, B. (2006). Micropropagation of rough lemon (Citrus jambhiri Lush.): Effect of explanttype and hormone concentration. Acta Botanica Croatica 65(2), 137-146.
  • Ak, B.E., Karahan Kiyar, P., Hatipoglu, I.H., Dikmetas, B. (2021). Effects of Different BA and IBA Concentrations on Proliferation and Rooting of ‘Garnem’ Rootstock In-vitro Propagation. International Journal of Agriculture Environment and Food Sciences, 5(4):470-476, DOI: 10.31015/jaefs.2021.4.6
  • Amgai, R.B., Prasai, H.K., Pandey, Y.R. (2016). Hormonal effect on mandarin orange (Citrusreticulata Blanco) micropropagation. Nepal Journal of Biotechnology, 4(1), 33-36. doi: 10.3126/njb.v4i1.16344
  • Babaoglu, M., Gurel, E. Ozcan, S. (2002). Bitki Biyoteknolojisi. Selçuk Üniversitesi Vakfı Yayınları, 374s.
  • Cengiz, M., Aka Kacar, Y. (2019). Micropropagation of Some Citrus Rootstocks with Classical and New Generation Tissue Culture Techniques. Turkish Journal of Agriculture - Food Science and Technology 7(9):1469.
  • Chamandoosti (2017).International Journal of Environmental&AgricultureResearch (IJOEAR).2454-1850
  • Espinosa, A.C., Pijut, P.M., Michler, C.H. (2006). Adventitious Shoot Regeneration and Rooting of Prunus serotina in vitro Cultures. Hortscience, 41(1): 193-201.
  • Gurel, S., Gulsen, Y. (1998). The Effects of IBA and BAP on in vitro Shoot Production of Almond (Amygdalus communis L.).Tr. J. of Botany, 22: 375-379.
  • Hatipoglu, R. (1999). Bitki Biyoteknolojisi. Çukurova Üniv. Zir. Fak. Genel Yayın No:190, Ders Kitabı Yayın No: A-58, Adana,176s.
  • Iqbal, N., Khan, N. A., Ferrante, A., Trivellini, A., Francini, A., Khan, M.I.R. (2017). Ethylene role in plant growth, development and senescence: interaction with other phytohormones. Frontiers in Plant Science, v.8, p.475, 2017. DOI: https://doi.org/10.3389/fpls.2017.00475
  • Jain, N., Babbar, S.B. (2003). Regeneration of “Juvenile” Plants of Black Plum, Syzygium cuminii Skeels, From Nodal Exp of Mature Trees. Plant Cell, Tissue and Organ Culture, 73: 257-263.
  • Kozai, T., Kubota, C., Jeong, B.R. (1997). Environmental Control For The Large-ScaleProduction Of Plants Through In Vitro Techniques. Plant Cell Tiss. Organ Culture, 51: 49-56.
  • Mansuroglu, S., Gurel, E. (2001). Bitki Biyoteknolojisi-I Doku Kültürü Ve Uygulamaları. Selçuk Üniversitesi Basımevi, Konya, 281s.
  • Muna, A.S., Ahmad, A.K., Mahmoud , K., Abdulrahman, K. (1999). In Vitro Propagation Of A Semi-Drawting Cherry Rootstock. Plant Cell and Tissue And Organ Culture, 59: 203- 208.
  • Nesom, G.L. (2014). Citrus trifoliata (Rutaceae): Review of biology and distribution in the USA. Phytoneuron 2014-46: 1–14. Published 1 May 2014. ISSN 2153 733X
  • Nikolić T. (2019): Flora Croatica – vaskularna flora Republike Hrvatske, Volumen 4. Ekskurzijska flora. Alfa d.d., Zagreb, v-x, 3-664.
  • Pruski, K., Astatkie, T., Nowak, J. (2005). Tissue Culture Propagation of Mongolian Cherry (Prunus fruticosa) and Nanking Cherry (Prunus tomentosa). Plant Cell, Tissue and Organ Culture, 82: 207-211.
  • Simsek, O. , Donmez, D., Aka Kaçar, Y. (2018). Bazı Turunçgil Anaçlarının In vitro Kuraklık Stresi Koşullarında Performanslarının Araştırılması. Yuzuncu Yıl University Journal of Agricultural Sciences, 28 (3) , 305-310 . DOI: 10.29133/yyutbd
  • Walsh, C.S. (2003). Plant Hormones. Concise Encyclopedia of Temperate Tree Fruit, Edited by Baugher T.A. and Singha, 245-250, ISBN 1560229411, Haworth Press.

Yıl 2022, Cilt: 6 Sayı: 4, 598 - 604, 30.12.2022

Elif Subasi Sevinc Bekir Erol Ak Ibrahim Halil Hatipoglu Heydem Ekinci

https://doi.org/10.31015/jaefs.2022.4.13

Öz

Proje Numarası

21096

Kaynakça

  • Ainsley, P.J., Collins, G.G., Sedgley, M. (2001a). In Vitro Rooting of Almond (Prunus dulcis Mill.). In vitro Cellular and Development Biology Plant, 37:778-785.
  • Ainsley, P.J., Hammerschlag, F.A., Bertozzi, T., Collins, G.G., Sedgley, M. (2001b). Regeneration of Almond from Immature Seed Cotyledons. Plant Cell, Tissue and Organ Culture, 67: 221-226.
  • Ali, S., Mirza, B. (2006). Micropropagation of rough lemon (Citrus jambhiri Lush.): Effect of explanttype and hormone concentration. Acta Botanica Croatica 65(2), 137-146.
  • Ak, B.E., Karahan Kiyar, P., Hatipoglu, I.H., Dikmetas, B. (2021). Effects of Different BA and IBA Concentrations on Proliferation and Rooting of ‘Garnem’ Rootstock In-vitro Propagation. International Journal of Agriculture Environment and Food Sciences, 5(4):470-476, DOI: 10.31015/jaefs.2021.4.6
  • Amgai, R.B., Prasai, H.K., Pandey, Y.R. (2016). Hormonal effect on mandarin orange (Citrusreticulata Blanco) micropropagation. Nepal Journal of Biotechnology, 4(1), 33-36. doi: 10.3126/njb.v4i1.16344
  • Babaoglu, M., Gurel, E. Ozcan, S. (2002). Bitki Biyoteknolojisi. Selçuk Üniversitesi Vakfı Yayınları, 374s.
  • Cengiz, M., Aka Kacar, Y. (2019). Micropropagation of Some Citrus Rootstocks with Classical and New Generation Tissue Culture Techniques. Turkish Journal of Agriculture - Food Science and Technology 7(9):1469.
  • Chamandoosti (2017).International Journal of Environmental&AgricultureResearch (IJOEAR).2454-1850
  • Espinosa, A.C., Pijut, P.M., Michler, C.H. (2006). Adventitious Shoot Regeneration and Rooting of Prunus serotina in vitro Cultures. Hortscience, 41(1): 193-201.
  • Gurel, S., Gulsen, Y. (1998). The Effects of IBA and BAP on in vitro Shoot Production of Almond (Amygdalus communis L.).Tr. J. of Botany, 22: 375-379.
  • Hatipoglu, R. (1999). Bitki Biyoteknolojisi. Çukurova Üniv. Zir. Fak. Genel Yayın No:190, Ders Kitabı Yayın No: A-58, Adana,176s.
  • Iqbal, N., Khan, N. A., Ferrante, A., Trivellini, A., Francini, A., Khan, M.I.R. (2017). Ethylene role in plant growth, development and senescence: interaction with other phytohormones. Frontiers in Plant Science, v.8, p.475, 2017. DOI: https://doi.org/10.3389/fpls.2017.00475
  • Jain, N., Babbar, S.B. (2003). Regeneration of “Juvenile” Plants of Black Plum, Syzygium cuminii Skeels, From Nodal Exp of Mature Trees. Plant Cell, Tissue and Organ Culture, 73: 257-263.
  • Kozai, T., Kubota, C., Jeong, B.R. (1997). Environmental Control For The Large-ScaleProduction Of Plants Through In Vitro Techniques. Plant Cell Tiss. Organ Culture, 51: 49-56.
  • Mansuroglu, S., Gurel, E. (2001). Bitki Biyoteknolojisi-I Doku Kültürü Ve Uygulamaları. Selçuk Üniversitesi Basımevi, Konya, 281s.
  • Muna, A.S., Ahmad, A.K., Mahmoud , K., Abdulrahman, K. (1999). In Vitro Propagation Of A Semi-Drawting Cherry Rootstock. Plant Cell and Tissue And Organ Culture, 59: 203- 208.
  • Nesom, G.L. (2014). Citrus trifoliata (Rutaceae): Review of biology and distribution in the USA. Phytoneuron 2014-46: 1–14. Published 1 May 2014. ISSN 2153 733X
  • Nikolić T. (2019): Flora Croatica – vaskularna flora Republike Hrvatske, Volumen 4. Ekskurzijska flora. Alfa d.d., Zagreb, v-x, 3-664.
  • Pruski, K., Astatkie, T., Nowak, J. (2005). Tissue Culture Propagation of Mongolian Cherry (Prunus fruticosa) and Nanking Cherry (Prunus tomentosa). Plant Cell, Tissue and Organ Culture, 82: 207-211.
  • Simsek, O. , Donmez, D., Aka Kaçar, Y. (2018). Bazı Turunçgil Anaçlarının In vitro Kuraklık Stresi Koşullarında Performanslarının Araştırılması. Yuzuncu Yıl University Journal of Agricultural Sciences, 28 (3) , 305-310 . DOI: 10.29133/yyutbd
  • Walsh, C.S. (2003). Plant Hormones. Concise Encyclopedia of Temperate Tree Fruit, Edited by Baugher T.A. and Singha, 245-250, ISBN 1560229411, Haworth Press.
Toplam 21 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Bitki Bilimi, Bahçe Bitkileri Yetiştirme ve Islahı
Bölüm Makaleler
Yazarlar

Elif Subasi Sevinc 0000-0001-5300-2534

Bekir Erol Ak 0000-0001-6938-942X

Ibrahim Halil Hatipoglu 0000-0002-7236-4976

Heydem Ekinci 0000-0002-1828-7367

Proje Numarası 21096
Yayımlanma Tarihi 30 Aralık 2022
Gönderilme Tarihi 25 Eylül 2022
Kabul Tarihi 27 Ekim 2022
Yayımlandığı Sayı Yıl 2022 Cilt: 6 Sayı: 4

Kaynak Göster

APA Subasi Sevinc, E., Ak, B. E., Hatipoglu, I. H., Ekinci, H. (2022). Effects of different combinations of growth regulators on the in vitro growth parameters of Poncirus trifoliata L. (Raf.). International Journal of Agriculture Environment and Food Sciences, 6(4), 598-604. https://doi.org/10.31015/jaefs.2022.4.13
AMA Subasi Sevinc E, Ak BE, Hatipoglu IH, Ekinci H. Effects of different combinations of growth regulators on the in vitro growth parameters of Poncirus trifoliata L. (Raf.). int. j. agric. environ. food sci. Aralık 2022;6(4):598-604. doi:10.31015/jaefs.2022.4.13
Chicago Subasi Sevinc, Elif, Bekir Erol Ak, Ibrahim Halil Hatipoglu, ve Heydem Ekinci. “Effects of Different Combinations of Growth Regulators on the in Vitro Growth Parameters of Poncirus Trifoliata L. (Raf.)”. International Journal of Agriculture Environment and Food Sciences 6, sy. 4 (Aralık 2022): 598-604. https://doi.org/10.31015/jaefs.2022.4.13.
EndNote Subasi Sevinc E, Ak BE, Hatipoglu IH, Ekinci H (01 Aralık 2022) Effects of different combinations of growth regulators on the in vitro growth parameters of Poncirus trifoliata L. (Raf.). International Journal of Agriculture Environment and Food Sciences 6 4 598–604.
IEEE E. Subasi Sevinc, B. E. Ak, I. H. Hatipoglu, ve H. Ekinci, “Effects of different combinations of growth regulators on the in vitro growth parameters of Poncirus trifoliata L. (Raf.)”, int. j. agric. environ. food sci., c. 6, sy. 4, ss. 598–604, 2022, doi: 10.31015/jaefs.2022.4.13.
ISNAD Subasi Sevinc, Elif vd. “Effects of Different Combinations of Growth Regulators on the in Vitro Growth Parameters of Poncirus Trifoliata L. (Raf.)”. International Journal of Agriculture Environment and Food Sciences 6/4 (Aralık 2022), 598-604. https://doi.org/10.31015/jaefs.2022.4.13.
JAMA Subasi Sevinc E, Ak BE, Hatipoglu IH, Ekinci H. Effects of different combinations of growth regulators on the in vitro growth parameters of Poncirus trifoliata L. (Raf.). int. j. agric. environ. food sci. 2022;6:598–604.
MLA Subasi Sevinc, Elif vd. “Effects of Different Combinations of Growth Regulators on the in Vitro Growth Parameters of Poncirus Trifoliata L. (Raf.)”. International Journal of Agriculture Environment and Food Sciences, c. 6, sy. 4, 2022, ss. 598-04, doi:10.31015/jaefs.2022.4.13.
Vancouver Subasi Sevinc E, Ak BE, Hatipoglu IH, Ekinci H. Effects of different combinations of growth regulators on the in vitro growth parameters of Poncirus trifoliata L. (Raf.). int. j. agric. environ. food sci. 2022;6(4):598-604.
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