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A study on bitter gourd (Momordica charantia L.) callogenesis optimization based on hormone balance and explant types

Yıl 2023, Cilt: 7 Sayı: 3, 633 - 638, 30.09.2023

Tugce Ozsan Kılıc Ahmet Naci Onus

https://doi.org/10.31015/jaefs.2023.3.17

Öz

Bitter gourd known as Momordica charantia L. is an important summer vegetable, belongs to the Cucurbitaceae family, grown in tropical areas in the world. Due to its fruits being rich in vitamins, minerals and good dietary fibers, the bitter gourd has many health-protective and pharmacological properties. The goal of the current study was to comparatively assess the indirect regeneration capability using in vivo leaf, internodal, and petiole explants of bitter gourd. To serve the purpose Murashige and Skoog (MS) media augmented with various concentrations of auxin and cytokinin for callus formation potent. The study was conducted by evaluating the selection of various auxin and cytokinin concentrations and combinations in MS basic medium. The rate of explant development decreased as cytokinin concentration increased. Although the lowest cytokinin concentration utilized in this study (1.0 mg L-1 BAP), it had a beneficial impact on explant development. When compared to the auxin NAA, the auxins IAA and 2,4-D were more beneficial on calli weights. It has been well proven that calli cannot be produced without the presence and balance of plant growth regulators. Experimental results demonstrated that callogenesis of bitter gourd from different explants might be successfully and effectively utilized in cell suspension cultures, genetic transformation, and callogenesis may also be adaptable to other species that are closely related.

Anahtar Kelimeler

Bitter Melon Calli Formation Internode Leaf Petiole Summer Vegetable

Kaynakça

  • Alexandra, F. & Dorica, B. (2010). Researches regarding bitter melon (Momordica charantia) in vitro regeneration. Journal of Horticulture, Forestry and Biotechnology, 14 (3), 75-80.
  • Alina, S., Dorica, B. & Ciulca, S. (2016). The influence of hormonal balance and in vitro culture duration on the Momordica charantia callus growing. Journal of Horticulture, Forestry and Biotechnology, 20 (2), 138-143.
  • Baharan, E., Mohammadi, P.P., Shahbazi, E., Hosseini, S.Z. (2015). Effects of some plant growth regulators and light on callus induction and explants browning in date palm (Phoenix dactylifera L.) in vitro leaves culture. Iranian Journal of Plant Physiology, 5 (4), 1473-1481.
  • Efferth, T. (2019). Biotechnology applications of plant callus cultures. Engineering, 5, 50-59. https://doi.org/10.1016/j.eng.2018.11.006
  • Elaleem, K.G.A., Modawi, R.S. & Khalafalla, M.M. (2009). Effect of plant growth regulators on callus induction and plant regeneration in tuber segment culture of potato (Solanum tuberosum L.) cultivar Diamant. African Journal of Biotechnology, 8 (11), 2529-2534. https://doi.org/10.13057/nusbiosci/n110209
  • Huda, A.K.M.N. & Sikdar, B. (2006). In vitro plant production through apical meristem culture of bitter gourd (Momordica charantia L.). Plant Tissue Culture & Biotechnology, 16 (1), 31-36. https://doi.org/10.3329/ptcb.v16i1.1103
  • Ill-Min Chung, I-M., Kaliyaperumal Rekha, K., Govindasamy Rajakumar, G. & Muthu Thiruvengadam, M. (2017). Jasmonic and salicylic acids enhanced phytochemical production and biological activities in cell suspension cultures of spine gourd (Momordica dioica Roxb). Acta Biologica Hungarica, 68 (1), 88-100. https://doi.org/10.1556/018.68.2017.1.8
  • Jeyaprakasam, A.J.S., Mahendhiran, M. & Palaniyandi, S.A. (2021). Development of protocols for in vitro culture of Momordica cymbalaria. Crop Research, 56 (5), 178-182. https://doi.org/10.31830/2454-1761.2021.029
  • Jin, W., Zhang, M. & Shi, W. (2019). Evaluation of ultrasound pretreatment and drying methods on selected quality attributes of bitter melon (Momordica charantia L.). Drying Technology, 37 (3), 387-396. https://doi.org/10.1080/07373937.2018.1458735
  • Lestari, N.K.D., Deswiniyanti, N.W., Astarini, I.A. & Arpiwi, L.M. (2019). Callus and shoot induction of leaf culture Lilium longiflorum with NAA and BAP. Nusantara Bioscience, 11 (2), 162-165. https://doi.org/10.13057/nusbiosci/n110209
  • Munsur, M.A.Z.A., Haque, M,S, Nasiruddin, K.M. & Hossain, M.S. (2009). In vitro propagation of bitter gourd (Momordica charantia L.) from nodal and root segments. Plant Tissue Culture & Biotechnology, 19 (1), 45-52. https://doi.org/10.3329/ptcb.v19i1.4916
  • Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  • Ozsan, T. & Onus, A.N. (2020). Callogenesis optimization of some globe artichoke [Cynara cardunculus var. scolymus (L.) Fiori] cultivars based on in vivo and in vitro leaf explants. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48 (4), 1873-1884. https://doi.org/10.15835/nbha48412089
  • Paul, A., Mitter, K. & Raychaudhuri, S.S. (2009). Effect of polyamines on in vitro somatic embryogenesis in Momordica charantia L. Plant Cell Tissue and Organ Culture 97, 303-311. https://doi.org/10.1007/s11240-009-9529-7
  • Siatka, T. (2019). Effects of growth regulators on production of anthocyanins in callus cultures of Angelica archangelica. Natural Product Communications 14 (6), 1-4. https://doi.org/10.1177/1934578X19857344
  • Sultana, R.S. & Rahman, M.M. (2012). Cells structure and morphogenesis of embryogenic aggregates in suspension culture of bitter melon (Momordica charantia L.). International Journal of Biosciences, 2 (3), 97-105.
  • Tang, Y., Liu, J., Liu, B., Li, X., Li, J. & Li, H. (2011). Additives promote adventitious buds induction from stem segments of bitter melon (Momordica charantia L.) Journal of Agricultural Science. 3 (2), 13-16. https://doi.org/10.5539/jas.v3n2p13
  • Tariq, U., Ali, M. & Abbasi, B.H. (2014). Morphogenic and biochemical variations under different spectral lights in callus cultures of Artemisia absinthium L. Journal of Photochemistry and Photobiology B: Biology 130:264-271. https://doi.org/10.1016/j.jphotobiol.2013.11.026
  • Thiruvengadam, M., Mohamed, S.V., Yang, C.H. & Jayabalan, N. (2006). Development of an embryogenic suspension culture of bitter melon (Momordica charantia L.). Scientia Horticulturae 109, 123–129. https://doi.org/10.1016/j.scienta.2006.03.012
  • Thiruvengadam, M., Rekha, K.T., Yang, C-H., Jayabalan, N. & Chung, I-M. (2010). High-frequency shoot regeneration from leaf explants through organogenesis in bitter melon (Momordica charantia L.). Plant Biotechnology Reports 4, 321–328. https://doi.org/10.1007/s11816-010-0151-2

Yıl 2023, Cilt: 7 Sayı: 3, 633 - 638, 30.09.2023

Tugce Ozsan Kılıc Ahmet Naci Onus

https://doi.org/10.31015/jaefs.2023.3.17

Öz

Kaynakça

  • Alexandra, F. & Dorica, B. (2010). Researches regarding bitter melon (Momordica charantia) in vitro regeneration. Journal of Horticulture, Forestry and Biotechnology, 14 (3), 75-80.
  • Alina, S., Dorica, B. & Ciulca, S. (2016). The influence of hormonal balance and in vitro culture duration on the Momordica charantia callus growing. Journal of Horticulture, Forestry and Biotechnology, 20 (2), 138-143.
  • Baharan, E., Mohammadi, P.P., Shahbazi, E., Hosseini, S.Z. (2015). Effects of some plant growth regulators and light on callus induction and explants browning in date palm (Phoenix dactylifera L.) in vitro leaves culture. Iranian Journal of Plant Physiology, 5 (4), 1473-1481.
  • Efferth, T. (2019). Biotechnology applications of plant callus cultures. Engineering, 5, 50-59. https://doi.org/10.1016/j.eng.2018.11.006
  • Elaleem, K.G.A., Modawi, R.S. & Khalafalla, M.M. (2009). Effect of plant growth regulators on callus induction and plant regeneration in tuber segment culture of potato (Solanum tuberosum L.) cultivar Diamant. African Journal of Biotechnology, 8 (11), 2529-2534. https://doi.org/10.13057/nusbiosci/n110209
  • Huda, A.K.M.N. & Sikdar, B. (2006). In vitro plant production through apical meristem culture of bitter gourd (Momordica charantia L.). Plant Tissue Culture & Biotechnology, 16 (1), 31-36. https://doi.org/10.3329/ptcb.v16i1.1103
  • Ill-Min Chung, I-M., Kaliyaperumal Rekha, K., Govindasamy Rajakumar, G. & Muthu Thiruvengadam, M. (2017). Jasmonic and salicylic acids enhanced phytochemical production and biological activities in cell suspension cultures of spine gourd (Momordica dioica Roxb). Acta Biologica Hungarica, 68 (1), 88-100. https://doi.org/10.1556/018.68.2017.1.8
  • Jeyaprakasam, A.J.S., Mahendhiran, M. & Palaniyandi, S.A. (2021). Development of protocols for in vitro culture of Momordica cymbalaria. Crop Research, 56 (5), 178-182. https://doi.org/10.31830/2454-1761.2021.029
  • Jin, W., Zhang, M. & Shi, W. (2019). Evaluation of ultrasound pretreatment and drying methods on selected quality attributes of bitter melon (Momordica charantia L.). Drying Technology, 37 (3), 387-396. https://doi.org/10.1080/07373937.2018.1458735
  • Lestari, N.K.D., Deswiniyanti, N.W., Astarini, I.A. & Arpiwi, L.M. (2019). Callus and shoot induction of leaf culture Lilium longiflorum with NAA and BAP. Nusantara Bioscience, 11 (2), 162-165. https://doi.org/10.13057/nusbiosci/n110209
  • Munsur, M.A.Z.A., Haque, M,S, Nasiruddin, K.M. & Hossain, M.S. (2009). In vitro propagation of bitter gourd (Momordica charantia L.) from nodal and root segments. Plant Tissue Culture & Biotechnology, 19 (1), 45-52. https://doi.org/10.3329/ptcb.v19i1.4916
  • Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  • Ozsan, T. & Onus, A.N. (2020). Callogenesis optimization of some globe artichoke [Cynara cardunculus var. scolymus (L.) Fiori] cultivars based on in vivo and in vitro leaf explants. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48 (4), 1873-1884. https://doi.org/10.15835/nbha48412089
  • Paul, A., Mitter, K. & Raychaudhuri, S.S. (2009). Effect of polyamines on in vitro somatic embryogenesis in Momordica charantia L. Plant Cell Tissue and Organ Culture 97, 303-311. https://doi.org/10.1007/s11240-009-9529-7
  • Siatka, T. (2019). Effects of growth regulators on production of anthocyanins in callus cultures of Angelica archangelica. Natural Product Communications 14 (6), 1-4. https://doi.org/10.1177/1934578X19857344
  • Sultana, R.S. & Rahman, M.M. (2012). Cells structure and morphogenesis of embryogenic aggregates in suspension culture of bitter melon (Momordica charantia L.). International Journal of Biosciences, 2 (3), 97-105.
  • Tang, Y., Liu, J., Liu, B., Li, X., Li, J. & Li, H. (2011). Additives promote adventitious buds induction from stem segments of bitter melon (Momordica charantia L.) Journal of Agricultural Science. 3 (2), 13-16. https://doi.org/10.5539/jas.v3n2p13
  • Tariq, U., Ali, M. & Abbasi, B.H. (2014). Morphogenic and biochemical variations under different spectral lights in callus cultures of Artemisia absinthium L. Journal of Photochemistry and Photobiology B: Biology 130:264-271. https://doi.org/10.1016/j.jphotobiol.2013.11.026
  • Thiruvengadam, M., Mohamed, S.V., Yang, C.H. & Jayabalan, N. (2006). Development of an embryogenic suspension culture of bitter melon (Momordica charantia L.). Scientia Horticulturae 109, 123–129. https://doi.org/10.1016/j.scienta.2006.03.012
  • Thiruvengadam, M., Rekha, K.T., Yang, C-H., Jayabalan, N. & Chung, I-M. (2010). High-frequency shoot regeneration from leaf explants through organogenesis in bitter melon (Momordica charantia L.). Plant Biotechnology Reports 4, 321–328. https://doi.org/10.1007/s11816-010-0151-2
Toplam 20 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Bitki Biyoteknolojisi
Bölüm Makaleler
Yazarlar

Tugce Ozsan Kılıc 0000-0002-3265-6886

Ahmet Naci Onus 0000-0001-8615-1480

Yayımlanma Tarihi 30 Eylül 2023
Gönderilme Tarihi 15 Ağustos 2023
Kabul Tarihi 14 Eylül 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 7 Sayı: 3

Kaynak Göster

APA Ozsan Kılıc, T., & Onus, A. N. (2023). A study on bitter gourd (Momordica charantia L.) callogenesis optimization based on hormone balance and explant types. International Journal of Agriculture Environment and Food Sciences, 7(3), 633-638. https://doi.org/10.31015/jaefs.2023.3.17
AMA Ozsan Kılıc T, Onus AN. A study on bitter gourd (Momordica charantia L.) callogenesis optimization based on hormone balance and explant types. int. j. agric. environ. food sci. Eylül 2023;7(3):633-638. doi:10.31015/jaefs.2023.3.17
Chicago Ozsan Kılıc, Tugce, ve Ahmet Naci Onus. “A Study on Bitter Gourd (Momordica Charantia L.) Callogenesis Optimization Based on Hormone Balance and Explant Types”. International Journal of Agriculture Environment and Food Sciences 7, sy. 3 (Eylül 2023): 633-38. https://doi.org/10.31015/jaefs.2023.3.17.
EndNote Ozsan Kılıc T, Onus AN (01 Eylül 2023) A study on bitter gourd (Momordica charantia L.) callogenesis optimization based on hormone balance and explant types. International Journal of Agriculture Environment and Food Sciences 7 3 633–638.
IEEE T. Ozsan Kılıc ve A. N. Onus, “A study on bitter gourd (Momordica charantia L.) callogenesis optimization based on hormone balance and explant types”, int. j. agric. environ. food sci., c. 7, sy. 3, ss. 633–638, 2023, doi: 10.31015/jaefs.2023.3.17.
ISNAD Ozsan Kılıc, Tugce - Onus, Ahmet Naci. “A Study on Bitter Gourd (Momordica Charantia L.) Callogenesis Optimization Based on Hormone Balance and Explant Types”. International Journal of Agriculture Environment and Food Sciences 7/3 (Eylül 2023), 633-638. https://doi.org/10.31015/jaefs.2023.3.17.
JAMA Ozsan Kılıc T, Onus AN. A study on bitter gourd (Momordica charantia L.) callogenesis optimization based on hormone balance and explant types. int. j. agric. environ. food sci. 2023;7:633–638.
MLA Ozsan Kılıc, Tugce ve Ahmet Naci Onus. “A Study on Bitter Gourd (Momordica Charantia L.) Callogenesis Optimization Based on Hormone Balance and Explant Types”. International Journal of Agriculture Environment and Food Sciences, c. 7, sy. 3, 2023, ss. 633-8, doi:10.31015/jaefs.2023.3.17.
Vancouver Ozsan Kılıc T, Onus AN. A study on bitter gourd (Momordica charantia L.) callogenesis optimization based on hormone balance and explant types. int. j. agric. environ. food sci. 2023;7(3):633-8.
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