Detection of Ten Resistance Genes Against P. syringae pv. phaseolicola and X. axonopodis pv. phaseoli in Twelve Local Bean Varieties Using SCAR Markers
Öz
Bean (Phaseolus vulgaris) is an important food and source of vitamin worldwide. It is preferred
in different cooking forms in different cultures. Some bacterial and fungal diseases cause serious loss in bean
production and impend nutrition and economy. In this study, we aimed to investigate the presence of some
resistance genes against two plant diseases (Pseudomonas syringae pv. phaseolicola and Xanthomonas axonopodis
pv. phaseoli) in twelve local bean varieties (Aslan, Elinda, İstanbul, Bursa, Flash, Belluga, White cordinal,
Beryl, Yunus-90, Göynük-98, Önceler-98, Eskişehir-855). We scanned four resistance genes (SR13, ST8, SH11
and SB10) for P. syringae pv. phaseolicola and six resistance genes (SAP6, BAC6, SU91, BC420, R7313 and
R4864) for X. axonopodis pv. phaseoli. PCR amplifcations were performed with specifc SCAR markers for each
resistance gene. The obtained DNA bands were scored as present or absent for the detection of resistance genes.
For comparison, the virulence rates of two plant diseases against twelve bean varieties were obtained from feld
results. We especially observed that two bean varieties (Aslan and Beryl) including four resistance genes against P.
syringae pv. phaseolicola are more resistant to this disease in feld. We revealed analysis results for all resistance
genes in twelve bean varieties and compared with resistance rates in feld. The data obtained from this study will
seriously be conduce to the improvement of resistant varieties against pathogens in bean production
Anahtar Kelimeler
PCR phaseolus vulgaris plant diseases resistance gene markers
Kaynakça
- Bai Y, Michaels TE, Pauls KP, 1997. Identification of RAPD markers linked to common bacterial blight resistance genes in Phaseolus vulgaris L. Genome, 40: 544-551.
- Beattie A, Michaels TE, Paul KP, 1998. An efficient reliable method to screen for common bacterial blight (CBB) resistance in Phaseolus vulgaris L. Annu. Rept. Bean Improv. Coop, 41: 53-54.
- Broughton WJ, Hernandez G, Blair M, Beebe S, Gepts P, Vanderleyden J, 2003. Beans (Phaseolus vulgaris) - Model Food Legumes. Plant and Soil, 252: 55-128.
- Çalışkan S, 2014. TR71 bölgesinde fasulye tarımının mevcut durumu, sorunları ve çözüm önerileri. Türk Tarım Gıda Bilim ve Teknoloji Dergisi, 2(2): 60-65.
- Carlos A, Urrea JR, Steadman MA, Pastor-Corrales DT, 2009. Lindgren, and J. P. Venegas, Registration of Great Northern common bean cultivar ‘coyne’ with enhanced disease resistance to common bacterial blight and bean rust. Journal of Plant Registrations. doi:10.3198/jpr2008.12.0692crc.
- Ceylan A, Öcal N, Akbulut M, 2014. Genetic diversity among the Turkish common bean cultivars (Phaseolus vulgaris L.) as assessed by SRAP, POGP and cpSSR markers. Biochemical Systematics and Ecology, 54: 219-229.
- Darkwa K, Ambachew D, Mohammed H, Asfaw A, Blair MW, 2016. Evaluation of common bean (Phaseolus vulgaris L.) genotypes for drought stress adaptation in Ethiopia. The crop journal, 4: 367-376.
- Doyle JJ, Doyle JL, 1987. A rapid DNA isolation procedure from small quantities of fresh leaf tissue. Phytochem. Bull., 19: 11-15.
- El-Garhy HAS, Rashid IAS, Abou-Ali RM, Moustafa, MMA, 2016. Field application of safe chemical elicitors induced the expression of some resistance genes against grey mold and cottony rot diseases during snap bean pods storage. Gene, 576: 358-365.
- FAO, 2014. Food and Agriculture Organization Statistical Database. http://faostat.fao.org/site/567/DesktopDefault.aspx? (Accessed Date: 13 October 2016).
- Fourie D, Miklas PN, Ariyarathne HM, 2004. Genes conditioning halo blight resistance to races 1, 7, and 9 occur in a tight cluster. Annu. Rep. Bean Improv. Coop., 47: 103-104.
- Freitas MB, Stadnik MJ, 2012. Race-specific and ulvan-induced defense responses in bean (Phaseolus vulgaris) against Colletotrichum lindemuthianum. Physiological and Molecular Plant Pathology, 78: 8-13.
- Friesen AP, Conner RL, Robinson DE, Barton WR, Gillard CL, 2014. Effect of microwave radiation on dry bean seed infected with Xanthomonas axonopodis pv. phaseoli with and without the use of chemical seed treatment. Crop Protection, 65: 77-85.
- Gururani MA, Venkatesh J, Upadhyaya CP, Nookaraju A, Pandey SK, Park SW, 2012. Plant disease resistance genes: Current status and future directions. Physiological and Molecular Plant Pathology, 78: 51-65.
- GTHB, 2016. Gıda Tarım ve Haycancılık Bakanlığı Bitkisel Üretim Genel Müdürlüğü “Yemeklik Baklagil Çalıştayı”.http://www.tarim.gov.tr/BUGEM/Belgeler/Duyurular/YEMEKLIK%20BAKLAGIL%2 0 CALISTAYI%20WEB%20i%C3%A7in%20Son.pdf, (Accessed Date: 13 October 2016).
- KFFABS, 2016. Karaman City Food, Farming and Animal Breeding Secretariat, http://karaman.tarim.gov.tr/Sayfalar/Detay.aspx?OgeId=23&Liste=Duyuru (Accessed Date: 13 October 2016).
- Jung G, Skroch PW, Nienhuis J, Coyne DP, Arnaud-Santana E, Ariyarathne HM, Marita JM, 1999. Confirmation of QTL associated with common bacterial blight resistance in four different genetic backgrounds in common bean. Crop Sci., 39: 1448-1455.
- Madakbaş S, Ellialtıoğlu Ş, 2012. Fasulye antraknozu (Colletotrichum lindemuthianum (Sacc. & Magnus) Lambs. Scrib.) hastalığına dayanıklılığın kalıtımı üzerine araştırmalar. Biyoloji Bilimleri Araştırma Dergisi, 5 (1): 99-109.
- Martins SR, Vences FJ, Sa´enz de Miera, LE, Barroso MR, Camide V, 2006. RAPD analysis of genetic diversity among and within Portuguese landraces of common white bean (Phaseolus vulgaris L.). Scientia Horticulturae, 108: 133–142.
- Meziadi C, Richard MMS, Derquennes A, Thareau A, Blanchet S, Gratias A, Pflieger S, Geffroy V, 2016. Development of molecular markers linked to disease resistance genesin common bean based on whole genome sequence. Plant Science, 242: 351-357.
- Miklas PN, Larsen R, Victry K, Delorme R, Marma C, Riley RH, Kelly JD, 2000. Marker-assisted selection for the bc-12 gene for resistance to BCMV and BCMNV in common bean. Euphytica, 116: 211-219.
- Miklas PN, Fourie D, Trapp J, Davis J, Myers JR. 2014. New loci including pse-6 conferring resistance to halo bacterial blight on chromosome pv04 in common bean. Crop Science. doi: 10.2135/cropsci2014.03.0213 .
- Osdaghi E, Rak MR, 2015. A source of resistance to bacterial wilt in the common bean (Phaseolus vulgaris) in Iran. Crop Protection, 74: 37-41.
- Pedraza F, Gallego G, Beebe S, Tohme J, 1997. Marcadores SCAR y RAPD para la resistencia a la bacteriosis comun (CBB). p.130-134. En Singh, S.P. y O. Voysest (eds.). Taller de mejoramiento de frijol para el Siglo XXI: Bases para una estrategia para America Latina. 559 pp. CIAT, Cali, Colombia.
- Perry G, DiNatale C, Xie W, Navabi A, Reinprecht Y, Crosby W, Yu K, Shi C, Pauls KP, 2013. A comparison of the molecular organization of genomic regions associated with resistance to common bacterial blight in two Phaseolus vulgaris genotypes, Frontiers in Plant. Science Plant Genetics and Genomics. doi: 10.3389/fpls.2013.00318.
- Raggi L, Tissi C, Mazzucato A, Negri V, 2014. Molecular polymorphism related to flowering trait variation in a Phaseolus vulgaris L. collection. Plant Science, 215-216: 180-189.
- Scarano D, Rubio F, Ruiz JJ, Rao R, Corrado G, 2014. Morphological and genetic diversity among and within common bean (Phaseolus vulgaris L.) landraces from the Campania region (Southern Italy). Scientia Horticulturae, 180: 72-78.
- Taylor JD, Teverson DM, Allen DJ, Pastor-Corrales MA, 1996. Identification and origin of races of Pseudomonas syringae pv phaseolicola from Africa and other bean growing areas. Plant Pathol., 45:469-478.
- TUIK, 2013. Bitkisel Üretim İstatistikleri. T.C. Başbakanlık Türkiye İstatistik Kurumu. http://www.tuik.gov.tr/itkiselapp/ bitkisel.zul, (Accessed Date: 13 October 2016).
- Turunç A, 2010. Klemantin mandarininde (Citrus clementina Blanco) SSR markörleri ile genetik haritalama. Master Thesis, Çukurova University, Adana.
- Xu S, Wang G, Mao W, Hu Q, Liu N, Ye L, Gong Y, 2014. Genetic diversity and population structure of common bean (Phaseolus vulgaris) landraces from China revealed by a new set of EST-SSR markers. Biochemical Systematics and Ecology, 57: 250-256.
- Yu K, Park SJ, Poysa V (2000) Marker-assisted selection of common beans for resistance to common bacterial blight: efficiency and economics. Plant Breed., 119: 411-416.
- Zhu J, Wu J, Wang L, Blair MW, Xhu Z, Wang S, 2016, QTL and candidate genes associated with common bacterial blight resistance in the common bean cultivar Longyundou 5 from China. The crop journal, 4, 344-352.
SCAR Markörler Kullanarak On İki Yerel Fasulye Çeşidinde P. Syringae Pv. Phaseolicola ve X. Axonopodis Pv. Phaseoli’ye Karşı On Direnç Geninin Tespit Edilmesi
Öz
Fasulye (Phaseolus vulgaris), dünya genelinde önemli bir besin ve vitamin kaynağıdır. Farklı kültürlerde
farklı yemek formları olarak tercih edilmektedir. Bazı bakteriyel ve fungal hastalıklar, fasulye üretiminde ciddi
kayıplara neden olmakta ve beslenme ve ekonomiyi tehdit etmektedir. Bu çalışmada, on iki yerel fasulye çeşidinde
(Aslan, Elinda, İstanbul, Bursa, Flash, Belluga, White cordinal, Beryl, Yunus-90, Göynük-98, Önceler-98,
Eskişehir-855), iki bitki hastalığına (Pseudomonas syringae pv. phaseolicola and Xanthomonas axonopodis pv.
phaseoli) karşı bazı direnç genleri belirlenmesini amaçlanmıştır. P. syringae pv. phaseolicola için dört direnç geni
(SR13, ST8, SH11 ve SB10) ve X. axonopodis pv. phaseoli için ise altı (SAP6, BAC6, SU91, BC420, R7313 ve
R4864) direnç geni taranmıştır. Her bir direnç geni için polimeraz zincir reaksiyonu (PCR)-çoğaltımları spesifk
SCAR belirteçler ile gerçekleştirilmiştir. Direnç genlerinin tespiti için elde edilen DNA bantları, var veya yok olarak
kaydedilmiştir. Karşılaştırma yapmak için, iki bitki hastalığına karşı hastalık yapma oranları tarla verilerinden elde
edilmiştir. Özellikle, P. syringae pv. phaseolicola’ya karşı dört direnç geni içeren iki fasulye türünün (Aslan ve
Beryl), tarlada bu hastalığa karşı daha dirençli olduğunu gözlenmiştir. On iki fasulye çeşidindeki tüm direnç genleri
için analiz sonuçlarını ortaya konulmuş ve tarladaki direnç oranları ile karşılaştırılmıştır. Bu çalışmadan elde edilen
veriler, fasulye üretiminde patojenlere karşı dirençli çeşitlerin geliştirilmesine ciddi bir katkı sağlayacaktır
Anahtar Kelimeler
Bitki Hastalıkları direnç gen belirteçleri pcr phaseolus vulgaris
Kaynakça
- Bai Y, Michaels TE, Pauls KP, 1997. Identification of RAPD markers linked to common bacterial blight resistance genes in Phaseolus vulgaris L. Genome, 40: 544-551.
- Beattie A, Michaels TE, Paul KP, 1998. An efficient reliable method to screen for common bacterial blight (CBB) resistance in Phaseolus vulgaris L. Annu. Rept. Bean Improv. Coop, 41: 53-54.
- Broughton WJ, Hernandez G, Blair M, Beebe S, Gepts P, Vanderleyden J, 2003. Beans (Phaseolus vulgaris) - Model Food Legumes. Plant and Soil, 252: 55-128.
- Çalışkan S, 2014. TR71 bölgesinde fasulye tarımının mevcut durumu, sorunları ve çözüm önerileri. Türk Tarım Gıda Bilim ve Teknoloji Dergisi, 2(2): 60-65.
- Carlos A, Urrea JR, Steadman MA, Pastor-Corrales DT, 2009. Lindgren, and J. P. Venegas, Registration of Great Northern common bean cultivar ‘coyne’ with enhanced disease resistance to common bacterial blight and bean rust. Journal of Plant Registrations. doi:10.3198/jpr2008.12.0692crc.
- Ceylan A, Öcal N, Akbulut M, 2014. Genetic diversity among the Turkish common bean cultivars (Phaseolus vulgaris L.) as assessed by SRAP, POGP and cpSSR markers. Biochemical Systematics and Ecology, 54: 219-229.
- Darkwa K, Ambachew D, Mohammed H, Asfaw A, Blair MW, 2016. Evaluation of common bean (Phaseolus vulgaris L.) genotypes for drought stress adaptation in Ethiopia. The crop journal, 4: 367-376.
- Doyle JJ, Doyle JL, 1987. A rapid DNA isolation procedure from small quantities of fresh leaf tissue. Phytochem. Bull., 19: 11-15.
- El-Garhy HAS, Rashid IAS, Abou-Ali RM, Moustafa, MMA, 2016. Field application of safe chemical elicitors induced the expression of some resistance genes against grey mold and cottony rot diseases during snap bean pods storage. Gene, 576: 358-365.
- FAO, 2014. Food and Agriculture Organization Statistical Database. http://faostat.fao.org/site/567/DesktopDefault.aspx? (Accessed Date: 13 October 2016).
- Fourie D, Miklas PN, Ariyarathne HM, 2004. Genes conditioning halo blight resistance to races 1, 7, and 9 occur in a tight cluster. Annu. Rep. Bean Improv. Coop., 47: 103-104.
- Freitas MB, Stadnik MJ, 2012. Race-specific and ulvan-induced defense responses in bean (Phaseolus vulgaris) against Colletotrichum lindemuthianum. Physiological and Molecular Plant Pathology, 78: 8-13.
- Friesen AP, Conner RL, Robinson DE, Barton WR, Gillard CL, 2014. Effect of microwave radiation on dry bean seed infected with Xanthomonas axonopodis pv. phaseoli with and without the use of chemical seed treatment. Crop Protection, 65: 77-85.
- Gururani MA, Venkatesh J, Upadhyaya CP, Nookaraju A, Pandey SK, Park SW, 2012. Plant disease resistance genes: Current status and future directions. Physiological and Molecular Plant Pathology, 78: 51-65.
- GTHB, 2016. Gıda Tarım ve Haycancılık Bakanlığı Bitkisel Üretim Genel Müdürlüğü “Yemeklik Baklagil Çalıştayı”.http://www.tarim.gov.tr/BUGEM/Belgeler/Duyurular/YEMEKLIK%20BAKLAGIL%2 0 CALISTAYI%20WEB%20i%C3%A7in%20Son.pdf, (Accessed Date: 13 October 2016).
- KFFABS, 2016. Karaman City Food, Farming and Animal Breeding Secretariat, http://karaman.tarim.gov.tr/Sayfalar/Detay.aspx?OgeId=23&Liste=Duyuru (Accessed Date: 13 October 2016).
- Jung G, Skroch PW, Nienhuis J, Coyne DP, Arnaud-Santana E, Ariyarathne HM, Marita JM, 1999. Confirmation of QTL associated with common bacterial blight resistance in four different genetic backgrounds in common bean. Crop Sci., 39: 1448-1455.
- Madakbaş S, Ellialtıoğlu Ş, 2012. Fasulye antraknozu (Colletotrichum lindemuthianum (Sacc. & Magnus) Lambs. Scrib.) hastalığına dayanıklılığın kalıtımı üzerine araştırmalar. Biyoloji Bilimleri Araştırma Dergisi, 5 (1): 99-109.
- Martins SR, Vences FJ, Sa´enz de Miera, LE, Barroso MR, Camide V, 2006. RAPD analysis of genetic diversity among and within Portuguese landraces of common white bean (Phaseolus vulgaris L.). Scientia Horticulturae, 108: 133–142.
- Meziadi C, Richard MMS, Derquennes A, Thareau A, Blanchet S, Gratias A, Pflieger S, Geffroy V, 2016. Development of molecular markers linked to disease resistance genesin common bean based on whole genome sequence. Plant Science, 242: 351-357.
- Miklas PN, Larsen R, Victry K, Delorme R, Marma C, Riley RH, Kelly JD, 2000. Marker-assisted selection for the bc-12 gene for resistance to BCMV and BCMNV in common bean. Euphytica, 116: 211-219.
- Miklas PN, Fourie D, Trapp J, Davis J, Myers JR. 2014. New loci including pse-6 conferring resistance to halo bacterial blight on chromosome pv04 in common bean. Crop Science. doi: 10.2135/cropsci2014.03.0213 .
- Osdaghi E, Rak MR, 2015. A source of resistance to bacterial wilt in the common bean (Phaseolus vulgaris) in Iran. Crop Protection, 74: 37-41.
- Pedraza F, Gallego G, Beebe S, Tohme J, 1997. Marcadores SCAR y RAPD para la resistencia a la bacteriosis comun (CBB). p.130-134. En Singh, S.P. y O. Voysest (eds.). Taller de mejoramiento de frijol para el Siglo XXI: Bases para una estrategia para America Latina. 559 pp. CIAT, Cali, Colombia.
- Perry G, DiNatale C, Xie W, Navabi A, Reinprecht Y, Crosby W, Yu K, Shi C, Pauls KP, 2013. A comparison of the molecular organization of genomic regions associated with resistance to common bacterial blight in two Phaseolus vulgaris genotypes, Frontiers in Plant. Science Plant Genetics and Genomics. doi: 10.3389/fpls.2013.00318.
- Raggi L, Tissi C, Mazzucato A, Negri V, 2014. Molecular polymorphism related to flowering trait variation in a Phaseolus vulgaris L. collection. Plant Science, 215-216: 180-189.
- Scarano D, Rubio F, Ruiz JJ, Rao R, Corrado G, 2014. Morphological and genetic diversity among and within common bean (Phaseolus vulgaris L.) landraces from the Campania region (Southern Italy). Scientia Horticulturae, 180: 72-78.
- Taylor JD, Teverson DM, Allen DJ, Pastor-Corrales MA, 1996. Identification and origin of races of Pseudomonas syringae pv phaseolicola from Africa and other bean growing areas. Plant Pathol., 45:469-478.
- TUIK, 2013. Bitkisel Üretim İstatistikleri. T.C. Başbakanlık Türkiye İstatistik Kurumu. http://www.tuik.gov.tr/itkiselapp/ bitkisel.zul, (Accessed Date: 13 October 2016).
- Turunç A, 2010. Klemantin mandarininde (Citrus clementina Blanco) SSR markörleri ile genetik haritalama. Master Thesis, Çukurova University, Adana.
- Xu S, Wang G, Mao W, Hu Q, Liu N, Ye L, Gong Y, 2014. Genetic diversity and population structure of common bean (Phaseolus vulgaris) landraces from China revealed by a new set of EST-SSR markers. Biochemical Systematics and Ecology, 57: 250-256.
- Yu K, Park SJ, Poysa V (2000) Marker-assisted selection of common beans for resistance to common bacterial blight: efficiency and economics. Plant Breed., 119: 411-416.
- Zhu J, Wu J, Wang L, Blair MW, Xhu Z, Wang S, 2016, QTL and candidate genes associated with common bacterial blight resistance in the common bean cultivar Longyundou 5 from China. The crop journal, 4, 344-352.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Bölüm | Moleküler Biyoloji ve Genetik / Moleculer Biology and Genetic |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Haziran 2017 |
| Gönderilme Tarihi | 28 Ekim 2016 |
| Kabul Tarihi | 30 Ocak 2017 |
| Yayımlandığı Sayı | Yıl 2017 Cilt: 7 Sayı: 2 |