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Kompostlardan elde edilen antagonist bakteri izolatlarının kavun solgunluk hastalığı etmeni Fusarium oxysporum f.sp. melonis’e karşı in vitro antagonistik etkilerinin belirlenmesi

Yıl 2022, Cilt: 27 Sayı: 3, 565 - 577, 01.12.2022

Senem Özkaya Emine Mine Soylu

https://doi.org/10.37908/mkutbd.1139502
Cited By: 1

Öz

Amaç: Bu çalışmada zeytin, pamuk ve hayvansal atıklardan yapılmış 5 farklı komposttan antagonistik etkinliğe sahip bakterilerin izolasyonu, tanılanması ve kavun solgunluk hastalığı etmeni Fusarium oxysporum f. sp. melonis (Fom)’in misel gelişimini engellemeleri üzerine olan etkinlikleri in vitro koşullarda araştırılmıştır.

Yöntem ve Bulgular: Kompostların fermentasyon süresine bağlı olarak bakteriyel popülasyon sayısında artış görülmüştür. En yüksek bakteri popülasyonu fermentasyonun 3 ve 4. aylarında belirlenmiştir. Farklı kompost materyallerinden izole edilen antagonist adayı bakteri izolatları Yağ Asit-Metil Ester (FAME) profillerine göre Mikrobiyal Tanılama Sistemi (MIS) ile teşhis edilmiştir. Aday bakterilerin Fom’in misel gelişimini engelleme potansiyelleri ikili kültür testleri ile belirlenmiştir. Elde edilen aday bakteriyel izolatlar arasında 31 bakteri izolatı ikili kültür denemelerinde, Fom’in misel gelişimini engellenmesi üzerine değişik oranlarda antagonistik etkinlik göstermiştir. Antagonistik potansiyele sahip bakteri izolatlarının çoğunluğunu farklı Bacillus spp.’a ait izolatlar (% 73.3) oluşturmuştur. Farklı kompost materyallerinden izole edilen bakteriler arasında en yüksek antagonistik etki %48.33 engelleme oranı ile Entereobacter gergoviae K4B:4:7:1 izolatı tarafından gösterilmiş olup, bu izolatı sırasıyla Bacillus cereus K1B:4:8:1 (%47.5), Salmonella typhimurium K5B:1:4:3 (% 46.67), Bacillus amyloliquefaciens K5B:0:5:1 (%43.33) ve Bacillus subtilis K3B:4:8:1 (%40.83) izolatları izlemiştir.

Genel Yorum: Organik atıklardan hazırlanmış kompostlar toprak kökenli hastalıklara karşı etkin antagonistik etkiye sahip bakterilere konukçuluk etmektedir. Kompostların in vivo koşullarda Fom’e karşı kullanılma potansiyellerinin araştırılmasının yanı sıra buralardan biyolojik preparat olarak kullanıma en uygun olan bakteri izolatların seçimi ve hastalığı engellemede kullandıkları mekanizmaların belirlenmesi üzerine çalışmalar gelecekte sürdürülmelidir.

Çalışmanın Önemi ve Etkisi: Elde edilen sonuçlar, Fom’in biyolojik mücadelesinde antagonistik potansiyele sahip mikroorganizmaların yoğun olarak geliştiği kompostların seralar gibi sınırlı alana sahip yetiştirme alanlarında kullanılmasının etkin bir rol oynayabileceğini göstermiştir. 

Anahtar Kelimeler

Fusarium oxysporum f. sp. melonis kavun kompost antagonist biyolojik mücadele

Destekleyen Kurum

Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Komisyonu Başkanlığı

Proje Numarası

MKU BAP 1101Y0110

Teşekkür

Bu çalışma Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Komisyonu Başkanlığı tarafından finansal olarak desteklenmiştir (Proje Numarası: MKU BAP 1101Y0110).

Kaynakça

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  • Angelopoulou DJ, Naska EJ, Paplomatas EJ, Tjamos SE (2014) Biological control agents (BCAs) of V. dahliae wilt: influence of application rates and delivery method on plant protection, triggering of host defense mechanisms and rhizosphere populations of BCAs. Plant Pathol. 63: 1062–1069.
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  • Bora T, Ozaktan H, Göre E, Aslan E (2004) Biological control of Fusarium oxysporum f. sp. melonis by Wettable Powder formulations of the two strains of Pseudomonas putida. J. Phytopathol. 152: 471-475.
  • Bozkurt İA, Soylu S (2019) Elma kök uru hastalığı etmeni Rhizobium radiobacter’e karşı epifit ve endofit bakteri izolatlarının antagonistik potansiyellerinin belirlenmesi. Tekirdağ Zir. Fak. Derg. 16: 348-361.
  • Cavigelli MA, Thien SJ (2003) Phosphorus bioavailability following incorporation of green manure crops. Soil Sci. Soc. America J. 67: 1186-1194.
  • Chakraborty N, Chakraborty N, Acharyya P, Acharya K (2021) Isolation, characterization and identification of novel broad spectrum bacterial antagonist(s) to control Fusarium wilt of eggplant. Physiol. Molecular Plant Pathol. 116: 101711.
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  • Cruz-Martin M, Leyva L, Acosta-Suarez M, Pichardo T, Bermudez-Caraballoso I, Alvarado- Capo Y (2021) Antifungal activity of Bacillus amyloliquefaciens against Fusarium oxysporum f. sp. cubense race 1. Agronom. Mesoameric. 32: 466-478.
  • Delisoy K, Altınok HH 2019. Kavunda Fusarium solgunluk hastalığına karşı bazı rizobakterilerin ve bitki aktivatörlerinin etkinliklerinin belirlenmesi. Anadolu Tar. Bil. Derg. 34: 135-145.
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Determination of antagonistic effects antagonist bacterial isolates obtained from composts against melon wilt disease agent Fusarium oxysporum f.sp. melonis in vitro conditions

Yıl 2022, Cilt: 27 Sayı: 3, 565 - 577, 01.12.2022

Senem Özkaya Emine Mine Soylu

https://doi.org/10.37908/mkutbd.1139502
Cited By: 1

Öz

Aims: Isolation, identification and antagonistic potentials of bacteria obtained from 5 different composts, which were made from olive cake, cotton seed cake and animal manure, were investigated on inhibition of mycelial growth of the melon wilt disease agent Fusarium oxysporum f. sp. melonis (Fom) in vitro conditions.

Methods and Results: An increase in the number of bacterial populations was observed depending on the fermentation of the composts. The highest bacterial population was determined in 3rd and 4th months of fermentation. The putative antagonist bacterial isolates obtained from different compost materials were identified by Microbial Identification System (MIS) according to their Fatty Acid-Methyl Ester (FAME) profiles. The antagonistic potentials of bacteria against Fom were determined by dual culture tests. Among the putative bacterial isolates obtained, 31 bacterial isolates showed antagonistic activity on inhibition of mycelial growth of Fom at varying rates in dual culture tests. Most of the bacterial isolates having antagonistic potential were identified as Bacillus spp. (73.3%). Among the antagonistic bacteria isolated from different compost materials, the highest antagonistic effect was displayed by Enterobacter gergoviae K4B:4:7:1 with a 48.33% inhibition rate, and this isolate was followed by Bacillus cereus K1B:4:8:1 (47.5%), Salmonella typhimurium K5B:1:4:3 (46.67%), Bacillus amyloliquefaciens K5B:0:5:1 (43.33%) and Bacillus subtilis K3B:4:8:1 (40.83%) isolates, respectively.

Conclusions: Composts prepared from organic wastes host effective bacteria that have an antagonistic effect against soil-borne diseases. Future studies should be conducted to investigate the in vivo potential of composts against Fom, to select the most suitable bacterial isolates for use as biological preparations, and to identify mechanisms used to prevent disease development.

Significance and Impact of the Study: The results indicated that the use of organic waste composts, where microorganisms with antagonistic potential are intensively developed, can play an effective role in growing areas with limited space such as greenhouses.

Anahtar Kelimeler

Fusarium oxysporum f. sp. melonis melon compost antagonist biological control

Proje Numarası

MKU BAP 1101Y0110

Kaynakça

  • Alimi M, Soleimani MJ, Darzi MT (2012) Characterization and application of microbial antagonists for control of Fusarium head blight of wheat caused by Fusarium graminearum using single and mixture strain of antagonistic bacteria on resistance and susceptible cultivars. African J. Microbiol. Res. 6: 326-334.
  • Angelopoulou DJ, Naska EJ, Paplomatas EJ, Tjamos SE (2014) Biological control agents (BCAs) of V. dahliae wilt: influence of application rates and delivery method on plant protection, triggering of host defense mechanisms and rhizosphere populations of BCAs. Plant Pathol. 63: 1062–1069.
  • Anonymous (2020) Food and Agriculture Organization of the United Nations. https://www.fao.org Erişim tarihi 10.03.2022.
  • Avilés M, Borrero C, Trillas MI (2011) Review on compost as an inducer of disease suppression in plants grown in soilless culture. Dyn. Soil Dyn. Plant 5 (2): 1–11.
  • Bonanomi G, Antignani V, Pane C, Scala F (2007) Suppression of soil borne fungal diseases with organic amendments. J. Plant Pathol. 89: 311–324.
  • Bora T, Ozaktan H, Göre E, Aslan E (2004) Biological control of Fusarium oxysporum f. sp. melonis by Wettable Powder formulations of the two strains of Pseudomonas putida. J. Phytopathol. 152: 471-475.
  • Bozkurt İA, Soylu S (2019) Elma kök uru hastalığı etmeni Rhizobium radiobacter’e karşı epifit ve endofit bakteri izolatlarının antagonistik potansiyellerinin belirlenmesi. Tekirdağ Zir. Fak. Derg. 16: 348-361.
  • Cavigelli MA, Thien SJ (2003) Phosphorus bioavailability following incorporation of green manure crops. Soil Sci. Soc. America J. 67: 1186-1194.
  • Chakraborty N, Chakraborty N, Acharyya P, Acharya K (2021) Isolation, characterization and identification of novel broad spectrum bacterial antagonist(s) to control Fusarium wilt of eggplant. Physiol. Molecular Plant Pathol. 116: 101711.
  • Cheuk W, Kwang VL, Copeman R, Joliffe P, Fraser S (2005) Disease suppression on greenhouse tomatoes using plant waste compost. J. Environ. Sci. Health Part B, 40: 449-461.
  • Chung YR, Hoitink HAJ (1990) Interactions between thermophilic fungi and Trichoderma hamatum in suppression of Rhizoctonia damping-off in a bark compost-amended container medium. Phytopathology, 80 : 73-77. Conklin AE, Susan ME, Liebman M, Lambert ED, Gallandt R, William Halteman A (2002) Effects of red clover (Trifolium pratense) green manure and compost soil amendments on wild mustard (Brassica kaber) growth and incidence of disease. Plant Soil. 238: 245-256.
  • Corato UD, Salimbeni R, Pretis AD, Patruno L, Avella N, Lacolla G, Cucci G (2018) Microbiota from ‘next-generation green compost’ improves suppressiveness of composted Municipal-Solid-Waste to soil-borne plant pathogens. Biological Control 124: 1–17.
  • Cruz-Martin M, Leyva L, Acosta-Suarez M, Pichardo T, Bermudez-Caraballoso I, Alvarado- Capo Y (2021) Antifungal activity of Bacillus amyloliquefaciens against Fusarium oxysporum f. sp. cubense race 1. Agronom. Mesoameric. 32: 466-478.
  • Delisoy K, Altınok HH 2019. Kavunda Fusarium solgunluk hastalığına karşı bazı rizobakterilerin ve bitki aktivatörlerinin etkinliklerinin belirlenmesi. Anadolu Tar. Bil. Derg. 34: 135-145.
  • Dukare AS, Prasanna R, Dubey SC, Nain L, Chaudhary V, Singh R, Saxena AK (2011) Evaluating novel microbe amended composts as biocontrol agents in tomato. Crop Protection, 30: 436-442.
  • Duniway JM (2002) Status of chemical alternatives to methyl bromide for preplant fumigation of soil. Phytopathology, 92: 1337- 1343.
  • Emmert EAB, Handelsman J (1999) Biocontrol of plant disease: a (Gram -) positive perspective. FEMS Microbiol. Lett. 171: 1-9.
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  • Sadfi N, Cherif M, Hajlaoui MR, Boudabbous A (2002) Biological control of the potato tubers dry rot caused by Fusarium roseum var. sambucinum under greenhouse, field and storage conditions using Bacillus spp. isolates. J. Phytopathol. 150: 640-648.
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  • Zitter TA, Hopkins DL, Thomas CE (1996) Compendium of cucurbit diseases. APS Press, St. Paul.
Toplam 71 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Ziraat Mühendisliği
Bölüm Araştırma Makalesi
Yazarlar

Senem Özkaya 0000-0001-6703-481X

Emine Mine Soylu 0000-0001-5961-0848

Proje Numarası MKU BAP 1101Y0110
Yayımlanma Tarihi 1 Aralık 2022
Gönderilme Tarihi 1 Temmuz 2022
Kabul Tarihi 3 Ağustos 2022
Yayımlandığı Sayı Yıl 2022 Cilt: 27 Sayı: 3

Kaynak Göster

APA Özkaya, S., & Soylu, E. M. (2022). Kompostlardan elde edilen antagonist bakteri izolatlarının kavun solgunluk hastalığı etmeni Fusarium oxysporum f.sp. melonis’e karşı in vitro antagonistik etkilerinin belirlenmesi. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 27(3), 565-577. https://doi.org/10.37908/mkutbd.1139502

Cited By

Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris
Harran Tarım ve Gıda Bilimleri Dergisi
https://doi.org/10.29050/harranziraat.1216304
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