Araştırma Makalesi
Patateste Tuz Stresi Altında In Vitro Mikro Yumru Üretimine Yönelik Karbon Kaynağı Tiplerinin Etkinliklerinin Araştırılması
Öz
Patatesin
mikro tuberizasyonu karmaşık fizyolojik bir süreç olup, karbon kaynakları patateste
mikrotuberizasyonu etkileyen en önemli faktörlerden biridir. Bitkilerde tuz
stresi altında glikoz, fruktoz, sukroz gibi şekerler biriktirilmekte, bu
moleküller ozmotik dengeleme, karbon depolama ve oksijen radikallerinin elemine
edilmesinde görev almaktadır. Bu çalışmanın amacı da, karbon kaynağı olarak
kullanılan sukroz, glikoz ve fruktozun in
vitro koşullarında tuz stresi altında, mikrotuberizasyonu nasıl
etkilediğinin ortaya konulmasıdır. Normal şartlarda ve tuz stresi altında en iyi
mikro yumru oluşturma kapasitesinin 40+40 g/l sukroz uygulamasında elde edildiği,
başlangıç karbonhidrat kaynağı olarak sukroza eklemlenen küçük molekül
ağırlığına sahip diğer şeker türevlerinin mikro yumru oluşturma kapasitesini olumlu
etkilemediği ortaya konmuştur.
Anahtar Kelimeler
Kaynakça
- Ashraf M, 2004. Some important physiological selection criteria for salt tolerance in plants. Flora, 199:361-376.
- Ashraf M, Haris P J C, 2004. Potential biochemical indicators of salinity tolerance in plants Plant Science, 166: 3-16.
- Chandra R, Dodds J H, Tovar P, 1988. In vitro tuberization in Potato (Solanum tuberosum L.). International Association of Plant Tissue Culture Newsletter,55:10-20.
- Charles G, Rossingol L, Rossingol M, 1992. Environmental effect on potato plants in vitro. J of Plant Physiology, 6: 708-713.
- Choi K H, Jeon J H, Kim H S, Joung Y H, Joung H, Choi K H, Leon J H, Kim H S, Joung H, Joung H, 1999. Stability of transgenic potato plants and their progenies pressing herbicide resistant gene. J KoreanSoc. Hort. Sci, 40:31-34.
- Choi K H, Jeon J H, Kim H S, Joung Y H, Joung H, Lim Y P, 1997. Genetic transformation of intact potato microtuber by particle bombardment. Korean J. Plant Tissue Culture, 24:87-91.
- Çulha Ş, Çakırlar H, 2011. Tuzluluğun bitkiler üzerine etkileri ve tuz tolerans mekanizmaları. AKÜ FEBİD, 11: 11-34.
- Deryabin A N, Yur’eva N O, 2010. Exogenous regulation of tuberization of solanum tuberosum L. culture in vitro (Review). Celckoxozyayctvennaya Biologiya Сельскохозяйственнаябиология, 3: 17-25.
- Harmey M A, Crowley M P, Clinch P E M, 1966. The Effect of Growth Regulators on Tuberisation of Cultured Stem Pieces of Solanum Tuberosum. European Potato J., 9:146-151.
- Inui H, Ueyama Y, Shiota N, Ohkawa Y, Ohkawa H, 1999. Herbicide metabolism and cross-tolerance in transgenic potato plants expressing human CYP1A1. Pesticide Biochemistry and Physiolog, 64:33-46.
- Kaur S, Mukerji K G, 2004. Potato diseases and their management. Disease management of fruitsand vegetables-1, fruit and vegetable diseases Mukerji K G (Ed.), Kluwer Academic Publishers, Netherlands, p.554.
- Khuri S, Moorby J, 1995. Investigations in to the role of sucrose in potato cv. Estima microtuber production in vitro. Annals of Botany, 75:295-203.
- Kumlay A M, Arslan N, Kaya C, 2014. Patates (Solanum Tuberosum L.)’Te in vitro şartlarda mikroyumru elde edilmesini etkileyen faktörler. Anadolu Tarım Bilim. Derg, 29(2): 154-165.
- Munns R, 2005. Genesand salt tolerance: bringing the mtogether. New Phytologist, 167:645-663.
- Murashige T, Skoog F, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant, 15: 473-497.
- O’Brien P J, Allen E J, Firman D M. 1998. A review of some studies into tuber initiation in potato (Solanum tuberosum L.) crops. J of Agric. Sci., 130: 251-270.
- Parida A K, Das A B, 2005. Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety, 60: 324-349.
- Parvaiz A, Satyawati S, 2008. Salt stress and phyto-biochemical responses of plants-a review. Plant Soil Environment, 54 (3): 89-99.
- Rahman M H, Islam R, Hossain M, Islam M S, 2010. Role of sucrose, glucose and maltose on conventional potato micropropagation. Journal of Agricultural Technology, 6(4): 733-739.
- Sandhu J S, Webster C I, Gray J C, I998. AIrrichsequencesact as quantitative enhancer of gene expression in transgenic tobacco and potato plants. Plant Mol BioI, 37:885-896.
- Seetohul S, 1995. A Study of The Effects of Carbohydrates in Tissue Culture of Nicotiana Tabacum. Dissertation for Requirement of B.Sc, University of Mauritius, Mauritius.
- Tuteja N, 2007. Mechanisms of High Salinity Tolerance in Plants. Methods in Enzymology, 428: 419-438.
- Wang P, Hu C, 1982. In vitro Mass Tuberization and Virus-Free Seed Potato Production in Taiwan. American Potato J., 59:33-37.
- Yasmin, A.,Jalbani, A.A., Mangrio, G.S., Nasreen, A. 2011. Optimization of microtuberization in indigenouspotato cv. Desiree. Pak. J. Biotechnol. 8(2): 39-44.
- Yılmaz, E. Tuna L.A., Bürün B., 2011. Bitkilerin Tuz Stresi Etkilerine Karsı Gelistirdikleri Tolerans Stratejileri.C.B.Ü. Fen Bilimleri Dergisi, 7.1 (2011) 47–66.
- Yu, W.C.,Xing,Y., andLuo, Y., 2000. Sucroseunilizasyonduringpotatomicrotubergrowth in bioreactor. Plant Cell Reports, 19:407-413.
- Zhu, J-K., 2002. Salt and drought stress signal transductıon in plants, AnnualReview of PlantBiology, 53, 247-73.
Yıl 2017,
Cilt: 22 Sayı: 1, 48 - 53, 30.06.2017
Öz
Kaynakça
- Ashraf M, 2004. Some important physiological selection criteria for salt tolerance in plants. Flora, 199:361-376.
- Ashraf M, Haris P J C, 2004. Potential biochemical indicators of salinity tolerance in plants Plant Science, 166: 3-16.
- Chandra R, Dodds J H, Tovar P, 1988. In vitro tuberization in Potato (Solanum tuberosum L.). International Association of Plant Tissue Culture Newsletter,55:10-20.
- Charles G, Rossingol L, Rossingol M, 1992. Environmental effect on potato plants in vitro. J of Plant Physiology, 6: 708-713.
- Choi K H, Jeon J H, Kim H S, Joung Y H, Joung H, Choi K H, Leon J H, Kim H S, Joung H, Joung H, 1999. Stability of transgenic potato plants and their progenies pressing herbicide resistant gene. J KoreanSoc. Hort. Sci, 40:31-34.
- Choi K H, Jeon J H, Kim H S, Joung Y H, Joung H, Lim Y P, 1997. Genetic transformation of intact potato microtuber by particle bombardment. Korean J. Plant Tissue Culture, 24:87-91.
- Çulha Ş, Çakırlar H, 2011. Tuzluluğun bitkiler üzerine etkileri ve tuz tolerans mekanizmaları. AKÜ FEBİD, 11: 11-34.
- Deryabin A N, Yur’eva N O, 2010. Exogenous regulation of tuberization of solanum tuberosum L. culture in vitro (Review). Celckoxozyayctvennaya Biologiya Сельскохозяйственнаябиология, 3: 17-25.
- Harmey M A, Crowley M P, Clinch P E M, 1966. The Effect of Growth Regulators on Tuberisation of Cultured Stem Pieces of Solanum Tuberosum. European Potato J., 9:146-151.
- Inui H, Ueyama Y, Shiota N, Ohkawa Y, Ohkawa H, 1999. Herbicide metabolism and cross-tolerance in transgenic potato plants expressing human CYP1A1. Pesticide Biochemistry and Physiolog, 64:33-46.
- Kaur S, Mukerji K G, 2004. Potato diseases and their management. Disease management of fruitsand vegetables-1, fruit and vegetable diseases Mukerji K G (Ed.), Kluwer Academic Publishers, Netherlands, p.554.
- Khuri S, Moorby J, 1995. Investigations in to the role of sucrose in potato cv. Estima microtuber production in vitro. Annals of Botany, 75:295-203.
- Kumlay A M, Arslan N, Kaya C, 2014. Patates (Solanum Tuberosum L.)’Te in vitro şartlarda mikroyumru elde edilmesini etkileyen faktörler. Anadolu Tarım Bilim. Derg, 29(2): 154-165.
- Munns R, 2005. Genesand salt tolerance: bringing the mtogether. New Phytologist, 167:645-663.
- Murashige T, Skoog F, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant, 15: 473-497.
- O’Brien P J, Allen E J, Firman D M. 1998. A review of some studies into tuber initiation in potato (Solanum tuberosum L.) crops. J of Agric. Sci., 130: 251-270.
- Parida A K, Das A B, 2005. Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety, 60: 324-349.
- Parvaiz A, Satyawati S, 2008. Salt stress and phyto-biochemical responses of plants-a review. Plant Soil Environment, 54 (3): 89-99.
- Rahman M H, Islam R, Hossain M, Islam M S, 2010. Role of sucrose, glucose and maltose on conventional potato micropropagation. Journal of Agricultural Technology, 6(4): 733-739.
- Sandhu J S, Webster C I, Gray J C, I998. AIrrichsequencesact as quantitative enhancer of gene expression in transgenic tobacco and potato plants. Plant Mol BioI, 37:885-896.
- Seetohul S, 1995. A Study of The Effects of Carbohydrates in Tissue Culture of Nicotiana Tabacum. Dissertation for Requirement of B.Sc, University of Mauritius, Mauritius.
- Tuteja N, 2007. Mechanisms of High Salinity Tolerance in Plants. Methods in Enzymology, 428: 419-438.
- Wang P, Hu C, 1982. In vitro Mass Tuberization and Virus-Free Seed Potato Production in Taiwan. American Potato J., 59:33-37.
- Yasmin, A.,Jalbani, A.A., Mangrio, G.S., Nasreen, A. 2011. Optimization of microtuberization in indigenouspotato cv. Desiree. Pak. J. Biotechnol. 8(2): 39-44.
- Yılmaz, E. Tuna L.A., Bürün B., 2011. Bitkilerin Tuz Stresi Etkilerine Karsı Gelistirdikleri Tolerans Stratejileri.C.B.Ü. Fen Bilimleri Dergisi, 7.1 (2011) 47–66.
- Yu, W.C.,Xing,Y., andLuo, Y., 2000. Sucroseunilizasyonduringpotatomicrotubergrowth in bioreactor. Plant Cell Reports, 19:407-413.
- Zhu, J-K., 2002. Salt and drought stress signal transductıon in plants, AnnualReview of PlantBiology, 53, 247-73.
Toplam 27 adet kaynakça vardır.
Ayrıntılar
| Bölüm | Araştırma Makalesi |
|---|---|
| Yazarlar | |
| Yayımlanma Tarihi | 30 Haziran 2017 |
| Yayımlandığı Sayı | Yıl 2017 Cilt: 22 Sayı: 1 |
