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Pregabalinin Gastrik Ülser Oluşumu ve Antioksidan Parametreler Üzerine Etkileri

Yıl 2020, Cilt: 42 Sayı: 1, 27 - 33, 01.01.2020
https://doi.org/10.20515/otd.475139

Öz

Pregabalin epilepsi, ankisiyete
ve nöropatik ağrıda kullanılan analjezik etkiye sahip antiinflamatuvar bir
ajandır. Biz çalışmamızda pregabalinin sıçanlarda mide üzerine yan etkilerinin
olup olmadığını ve bir non-steridal antiinflamatuvar bir ajan ile etkisini
karşılaştırmayı amaçladık. Pregabalinin gastrik mukozal hasara karşı koruyucu
olduğu düşünülen antioksidan seviyeleri üzerine olan etkilerini de araştırdık.
Pregabalin 30, 50, 100 mg/kg, indometasin 5 mg/kg (referans-NSAID), salin
(kontrol grubu) 10 gün boyunca oral olarak uygulandı. 10 günlük tedavinin
sonunda, sıçanlar sakrifiye edildi, mide dokuları çıkarıldı, mukus salgılanması
spektrofotometrik olarak belirlendi, ülser indeksi 0'dan (no-peteşi) skor 3'e
(peteşi> 5mm) kadar skorlandı. Ayrıca pregabalinin mide dokusundaki
antioksidan etkilerini değerlendirmek üzere malondialdehid (MDA), katalaz ve
süperoksit dismutaz (SOD) aktiviteleri çalışıldı. Pregabalin 50 mg/kg ve 100
mg/kg dozları kontrol grubu ile karşılaştırıldığında indometazine benzer
şekilde anlamlı olarak mukus salgılanmasını azalttı ve ülser indeksini artırdı.
Pregabalin 30 mg/kg dozunda bu etki görülmedi. Pregabalin 30 mg/kg ve 100 mg/kg
SOD ve katalaz seviyelerini düşürüyorken pregabalin 100
  mg/kg dozunda MDA seviyeleri artmıştır. 50
mg/kg and 100 mg/kg pregabalin mukus salgılanmasını ve ülser oluşumu
indometazin 30 mg/kg dozuna benzer şekilde azaltarak gastrik yan etkiler
göstermiştir. Pregabalin 30 mg/kg dozu ise gastrik yan etkilerin ortaya çıkmadığı
uygun doz olabilir. Pregabalin 50 mg/kg dozunun ise antioksidan seviyelerini
artırıcı etkiye sahip olduğu görülmüştür.

Kaynakça

  • 1. Eutamene H, Coelho AM, Theodorou V, Toulouse M, Chovet M, Doherty A, et al. Antinociceptive effect of pregabalin in septic shock-induced rectal hypersensitivity in rats. The Journal of pharmacology and experimental therapeutics. 2000;295(1):162-7.
  • 2. Ceyhan M. TE. Yeni Bir Antikonvülsan Pregabalin: Preklinik Veriler. Turk J Neurol. 2008;14(3):161-71.
  • 3. Moore RA, Straube S, Wiffen PJ, Derry S, McQuay HJ. Pregabalin for acute and chronic pain in adults. The Cochrane database of systematic reviews. 2009(3):Cd007076.
  • 4. Abou-Khalil BW. Antiepileptic Drugs. Continuum (Minneapolis, Minn). 2016;22(1 Epilepsy):132-56.
  • 5. Kaygisiz B, Kilic FS, Senguleroglu N, Baydemir C, Erol K. The antinociceptive effect and mechanisms of action of pregabalin in mice. Pharmacological reports : PR. 2015;67(1):129-33.
  • 6. Saritas TB, Korkmaz M, Sevimli A, Saritas ZK. Comparison of the effects of gabapentin and pregabalin on wound healing in rats. International wound journal. 2016;13(5):748-53.
  • 7. Buffum M, Buffum JC. Nonsteroidal anti-inflammatory drugs in the elderly. Pain management nursing : official journal of the American Society of Pain Management Nurses. 2000;1(2):40-50.
  • 8. Harirforoosh S, Asghar W, Jamali F. Adverse effects of nonsteroidal antiinflammatory drugs: an update of gastrointestinal, cardiovascular and renal complications. Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques. 2013;16(5):821-47.
  • 9. Fang YZ, Yang S, Wu G. Free radicals, antioxidants, and nutrition. Nutrition (Burbank, Los Angeles County, Calif). 2002;18(10):872-9.
  • 10. Halliwell B, Gutteridge JM. Oxygen free radicals and iron in relation to biology and medicine: some problems and concepts. Archives of biochemistry and biophysics. 1986;246(2):501-14.
  • 11. Yusuf S, Nok AJ, Ameh DA, Adelaiye AB, Balogun EO. Quantitative changes in gastric mucosal glycoproteins: effect of cholinergic agonist and vagal nerve stimulation in the rat. Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society. 2004;16(5):613-9.
  • 12. Sirmagul B, Kilic FS, Batu O, Erol K. The effects of verapamil on stress- and histamine-induced gastric lesions in rats. Methods and findings in experimental and clinical pharmacology. 2004;26(10):763-7.
  • 13. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical biochemistry. 1979;95(2):351-8.
  • 14. Zhang W, Liu K, Li L, Li Y, Sui X, Rao Y, et al. Therapeutic effect of low molecular weight chitosan containing sepia ink on ethanol-induced gastric ulcer in rats. Acta cirurgica brasileira. 2016;31(12):813-20.
  • 15. Winterbourn CC, Hawkins RE, Brian M, Carrell RW. The estimation of red cell superoxide dismutase activity. The Journal of laboratory and clinical medicine. 1975;85(2):337-41.
  • 16. Sinha M, Gautam L, Shukla PK, Kaur P, Sharma S, Singh TP. Current perspectives in NSAID-induced gastropathy. Mediators of inflammation. 2013;2013:258209.
  • 17. Blackler RW, Gemici B, Manko A, Wallace JL. NSAID-gastroenteropathy: new aspects of pathogenesis and prevention. Current opinion in pharmacology. 2014;19:11-6.
  • 18. Zidar N, Odar K, Glavac D, Jerse M, Zupanc T, Stajer D. Cyclooxygenase in normal human tissues--is COX-1 really a constitutive isoform, and COX-2 an inducible isoform? Journal of cellular and molecular medicine. 2009;13(9b):3753-63.
  • 19. Gudis K, Sakamoto C. The role of cyclooxygenase in gastric mucosal protection. Digestive diseases and sciences. 2005;50 Suppl 1:S16-23.
  • 20. Konturek SJ, Konturek PC, Pawlik T, Sliwowski Z, Ochmanski W, Hahn EG. Duodenal mucosal protection by bicarbonate secretion and its mechanisms. Journal of physiology and pharmacology : an official journal of the Polish Physiological Society. 2004;55 Suppl 2:5-17.
  • 21. Moreira Lda R, Brum Eda S, da Silva AR, de Freitas ML, Teixeira TP, Boligon AA, et al. Antinociceptive and anti-inflammatory effect of the Scutia buxifolia Reissek stem barks extract. Phytomedicine : international journal of phytotherapy and phytopharmacology. 2016;23(10):1021-8.
  • 22. Bernardi A, Zilberstein AC, Jager E, Campos MM, Morrone FB, Calixto JB, et al. Effects of indomethacin-loaded nanocapsules in experimental models of inflammation in rats. British journal of pharmacology. 2009;158(4):1104-11.
  • 23. Pandey A, Bani S, Satti NK, Gupta BD, Suri KA. Anti-arthritic activity of agnuside mediated through the down-regulation of inflammatory mediators and cytokines. Inflammation research : official journal of the European Histamine Research Society [et al]. 2012;61(4):293-304.

The Effects of Pregabalin on Gastric Ulcer Formation and Antioxidant Parameters

Yıl 2020, Cilt: 42 Sayı: 1, 27 - 33, 01.01.2020
https://doi.org/10.20515/otd.475139

Öz

Pregabalin, a drug used in
epilepsy, anxiety, neuropathic pain is reported to have analgesic effects in
inflammatory pain. 
We aimed to
investigate whether pregabalin have gastric side effects and to compare with a
non steroidal antiinflammatory drug (NSAID) in rats. The effects of pregabalin
on antioxidant levels, which are suggested to protect against gastric mucosal
damage were also studied. Pregabalin 30, 50, 100 mg/kg, indomethacin 5 mg/kg
(reference-NSAID), saline (control group) were administered orally for 10 days.
At the end of 10 day treatment, rats were sacrificed,
  gastric tissues were removed out,  mucus secretion was determined
spectrophotometrically, ulcer index was scored from score 0:(no-petechia) to
score 3:(petechia>5mm). Also, to evaluate the antioxidant effects of
pregabalin, malondialdehyde (MDA) levels, catalase and superoxide dismutase
(SOD) activities
  in gastric tissue were
studied. Pregabalin 50 mg/kg and 100 mg/kg similar to indomethacin
significantly reduced mucus secretion and increased ulcer index compared to
control while pregabalin 30 mg/kg did not. Pregabalin 30 mg/kg and 100 mg/kg
decreased SOD and catalase levels. Pregabalin 100 mg/kg dose increased MDA
levels. 50 mg/kg and 100 mg/kg pregabalin showed gastric side effects as
reduced mucus secretion and ulcer formation similar to indomethacin and 30
mg/kg pregabalin may be reasonable dose without showing gastric side effects.
Pregabalin 50 mg/kg seems to have enhancing effects on antioxidant levels.

Kaynakça

  • 1. Eutamene H, Coelho AM, Theodorou V, Toulouse M, Chovet M, Doherty A, et al. Antinociceptive effect of pregabalin in septic shock-induced rectal hypersensitivity in rats. The Journal of pharmacology and experimental therapeutics. 2000;295(1):162-7.
  • 2. Ceyhan M. TE. Yeni Bir Antikonvülsan Pregabalin: Preklinik Veriler. Turk J Neurol. 2008;14(3):161-71.
  • 3. Moore RA, Straube S, Wiffen PJ, Derry S, McQuay HJ. Pregabalin for acute and chronic pain in adults. The Cochrane database of systematic reviews. 2009(3):Cd007076.
  • 4. Abou-Khalil BW. Antiepileptic Drugs. Continuum (Minneapolis, Minn). 2016;22(1 Epilepsy):132-56.
  • 5. Kaygisiz B, Kilic FS, Senguleroglu N, Baydemir C, Erol K. The antinociceptive effect and mechanisms of action of pregabalin in mice. Pharmacological reports : PR. 2015;67(1):129-33.
  • 6. Saritas TB, Korkmaz M, Sevimli A, Saritas ZK. Comparison of the effects of gabapentin and pregabalin on wound healing in rats. International wound journal. 2016;13(5):748-53.
  • 7. Buffum M, Buffum JC. Nonsteroidal anti-inflammatory drugs in the elderly. Pain management nursing : official journal of the American Society of Pain Management Nurses. 2000;1(2):40-50.
  • 8. Harirforoosh S, Asghar W, Jamali F. Adverse effects of nonsteroidal antiinflammatory drugs: an update of gastrointestinal, cardiovascular and renal complications. Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques. 2013;16(5):821-47.
  • 9. Fang YZ, Yang S, Wu G. Free radicals, antioxidants, and nutrition. Nutrition (Burbank, Los Angeles County, Calif). 2002;18(10):872-9.
  • 10. Halliwell B, Gutteridge JM. Oxygen free radicals and iron in relation to biology and medicine: some problems and concepts. Archives of biochemistry and biophysics. 1986;246(2):501-14.
  • 11. Yusuf S, Nok AJ, Ameh DA, Adelaiye AB, Balogun EO. Quantitative changes in gastric mucosal glycoproteins: effect of cholinergic agonist and vagal nerve stimulation in the rat. Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society. 2004;16(5):613-9.
  • 12. Sirmagul B, Kilic FS, Batu O, Erol K. The effects of verapamil on stress- and histamine-induced gastric lesions in rats. Methods and findings in experimental and clinical pharmacology. 2004;26(10):763-7.
  • 13. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical biochemistry. 1979;95(2):351-8.
  • 14. Zhang W, Liu K, Li L, Li Y, Sui X, Rao Y, et al. Therapeutic effect of low molecular weight chitosan containing sepia ink on ethanol-induced gastric ulcer in rats. Acta cirurgica brasileira. 2016;31(12):813-20.
  • 15. Winterbourn CC, Hawkins RE, Brian M, Carrell RW. The estimation of red cell superoxide dismutase activity. The Journal of laboratory and clinical medicine. 1975;85(2):337-41.
  • 16. Sinha M, Gautam L, Shukla PK, Kaur P, Sharma S, Singh TP. Current perspectives in NSAID-induced gastropathy. Mediators of inflammation. 2013;2013:258209.
  • 17. Blackler RW, Gemici B, Manko A, Wallace JL. NSAID-gastroenteropathy: new aspects of pathogenesis and prevention. Current opinion in pharmacology. 2014;19:11-6.
  • 18. Zidar N, Odar K, Glavac D, Jerse M, Zupanc T, Stajer D. Cyclooxygenase in normal human tissues--is COX-1 really a constitutive isoform, and COX-2 an inducible isoform? Journal of cellular and molecular medicine. 2009;13(9b):3753-63.
  • 19. Gudis K, Sakamoto C. The role of cyclooxygenase in gastric mucosal protection. Digestive diseases and sciences. 2005;50 Suppl 1:S16-23.
  • 20. Konturek SJ, Konturek PC, Pawlik T, Sliwowski Z, Ochmanski W, Hahn EG. Duodenal mucosal protection by bicarbonate secretion and its mechanisms. Journal of physiology and pharmacology : an official journal of the Polish Physiological Society. 2004;55 Suppl 2:5-17.
  • 21. Moreira Lda R, Brum Eda S, da Silva AR, de Freitas ML, Teixeira TP, Boligon AA, et al. Antinociceptive and anti-inflammatory effect of the Scutia buxifolia Reissek stem barks extract. Phytomedicine : international journal of phytotherapy and phytopharmacology. 2016;23(10):1021-8.
  • 22. Bernardi A, Zilberstein AC, Jager E, Campos MM, Morrone FB, Calixto JB, et al. Effects of indomethacin-loaded nanocapsules in experimental models of inflammation in rats. British journal of pharmacology. 2009;158(4):1104-11.
  • 23. Pandey A, Bani S, Satti NK, Gupta BD, Suri KA. Anti-arthritic activity of agnuside mediated through the down-regulation of inflammatory mediators and cytokines. Inflammation research : official journal of the European Histamine Research Society [et al]. 2012;61(4):293-304.
Toplam 23 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm ORİJİNAL MAKALELER / ORIGINAL ARTICLES
Yazarlar

Bilgin Kaygisiz 0000-0001-5910-9914

Sule Aydin 0000-0003-2498-8378

Cafer Yildirim Bu kişi benim 0000-0002-1565-9217

Hadi Karimkhani 0000-0002-4966-1745

Setenay Oner 0000-0002-4759-4913

Fatma Sultan Kilic 0000-0002-5356-696X

Yayımlanma Tarihi 1 Ocak 2020
Yayımlandığı Sayı Yıl 2020 Cilt: 42 Sayı: 1

Kaynak Göster

Vancouver Kaygisiz B, Aydin S, Yildirim C, Karimkhani H, Oner S, Kilic FS. The Effects of Pregabalin on Gastric Ulcer Formation and Antioxidant Parameters. Osmangazi Tıp Dergisi. 2020;42(1):27-33.


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