Araştırma Makalesi
Investigation of Changes in Malondialdehyde Level, Superoxide Dismutase and Catalase Activity in Liver Tissue of Capoeta umbla exposed to 2,4-Dichlorophenoxyacetic Acid
Öz
In this study, changes in malondialdehyde level (MDA), superoxide dismutase (SOD) and catalase (CAT) activity in fish liver tissue were investigated by applying two doses of 2,4-dichlorophenoxyacetic acid (2,4-D) herbicide to Capoeta umbla fish for 72 hours. Changes in MDA level, CAT and SOD activity were determined by spectrophotometric methods. As a result of the study, it was determined that there was a statistically significant increase in MDA level, CAT and SOD activity in C. umbla liver tissue exposed to different doses of herbicide (p < 0.05). It was observed that 2,4-D herbicide disrupted the oxidant/antioxidant balance in fish liver tissue. However, these two antioxidant enzymes, which are the primary defense mechanism, appear to be resistant to the toxic effect of 2,4-D.
Anahtar Kelimeler
Capoeta umbla antioxidant enzymes liver herbicide oxidant/antioxidant balance
Kaynakça
- Adeyemi, J.A., Martins-Junior, A.C., Barbosa, J.F. 2015. Teratogenicity, genotoxicity and oxidative stress in zebrafish embryos (Danio rerio) co-exposed to arsenic and atrazine. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 7(12): 172-173.
- Aebi, H. 1974. Catalase invitro. In: Methods of enzymatic analysis, Ed: Bergmeyer HU, 2nd ed, FL pp:121-126.
- Fırat, Ö., Aytekin, T. 2018. Neonikotinoid insektisit thiamethoxamın Oreochromis niloticus’ta oksidatif stres parametreleri üzerine etkisi. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 20 (2): 224-234.
- Golombieski, J.I., Sutili, F.J., Salbego, J., Seben, D., Gressler, L.T., Arruda da Cunha, J., Gressler, L.T., Zanella, R., Vaucher, R.A., Marchesan, E., Baldisserotto, B. 2016. Imazapyr+imazapic herbicide determines acute toxicity in silver catfish Rhamdia quelen. Ecotoxicology and Environmental Safety, 128: 91-99.
- Gül S., Nur G., Kaya Ö.T. 2005. 2.4-D’nin Siraz Balığındaki (Capoeta capoeta umbla, Heckel 1843) LC50 Değeri. Türk Sucul Yaşam Dergisi, Sayı 4: 245–249.
- Husak, V.V., Mosiichuk, N.M., Maksymiv, I.V., Storey, J.M., Storey, K.B., Lushchak. V.I. 2016. Oxidative stress responses in gills of goldfish, Carassius auratus, exposed to the metribuzin-containing herbicide Sencor. Environmental Toxicology and Pharmacology, 45: 163- 169.
- Koç, N.D., Akbulut, C. 2012. Histological analysis of acute toxicity of 2,4-diclorophenoxyacetic acid in ovary of zebrafish, Animal Cells and Systems, 16: 400-407.
- Ledwozyw, A., Michalak, D., Stepien, A., Kadziolka, A. 1986. The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human atherosclerosis. Clinica Chimica Acta, 155(3): 275-283.
- Oliveira, J.M.M., Galhano, V., Henriques, I., Soares, A.M.V.M., Loureiro, S. 2017. Basagran® induces developmental malformations and changes the bacterial community of zebrafish embryos. Environmental Pollution, 221: 52-63.
- Oruç, E.Ö., Sevgiler, Y., Üner, N. 2004. Tissue-spesific oxidative stress responses in fish exposed to 2,4-D and azinphosmethyl. Comparative Biochemistry and Physiology, 137: 43-51.
- Özkan, H. 2017. Bazi insektisit ve herbisitlerin kitosan ile adsorpsiyonunun incelenmesi. Yüksek Lisans Tezi, Kimya Anabilim Dalı, Fen Bilimleri Enstitüsü, Süleyman Demirel Üniversitesi, Isparta.
- Persch, T.S.P., Weimer, R.N., Freitas, B.S., Oliveira, G.T. 2017. Metabolic parameters and oxidative balance in juvenile Rhamdia quelen exposed to rice paddy herbicides: Roundup®, Primoleo®, and Facet®. Chemosphere, 174: 98-109.
- Piancini, L.D.S., Guiloski, I.C., Silva de Assis, H.C., Cestari, M.M. 2015. Mesotrione herbicide promotes biochemical changes and DNA damage in two fish species. Toxicology Reports, 2: 1157-1163.
- Sun, Y., Oberley, L. W., Li, Y. 1988. A simple method for clinical assay of superoxide dismutase. Clinical Chemistry 34: 497–500.
- Tabassum, H., Ashafaq, M., Khan, J., Shah, Z., Raisuddin, S., Parvez, S. 2016. Short term exposure of pendimethalin induces biochemical and histological perturbations in liver, kidney and gill of freshwater fish. Ecological Indicators, 63: 29-36.
- Toroser, D., Orr, W.C., Sohal, R.S. 2007. Carbonylation of mitochondrial proteins in Drosophila melanogaster during aging, Biochemical and Biophysical Research Communications, 363: 418–424.
- Tutuş, R. 2016. Oreochromis niloticus’un karaciğer dokusundaki antioksidan sistemler ve lipid peroksidasyonu üzerine chlorpyrifos, emamectin benzoate ve abamectin türü pestisitlerin etkileri, Yüksek Lisans Tezi, Adıyaman Üniversitesi, Fen Bilimleri Enstitüsü, Adıyaman.
- Uçar, A., Al-Hamdani, A. H. A., Alak, G., Atamanalp, M., Topal, A., Arslan, H., Parlak, V., Fakioğlu, Ö., Şensurat, T. 2012. Karboksin’ in Gökkuşağı Alabalıkları(Oncorhynus Mykiss)’nda Süperoksitdismutaz Enzim Aktivitesi Üzerine Etkisi. Biyoloji Bilimleri Araştırma Dergisi 5(2): 83-85.
- Uslu, H., Atila, G. , Ersan, Y., Özden, Z. 2016. İsrail Sazanı (Carassius gibelio Bloch, 1782) Balıklarına Uygulanan Cypermethrin’in Oksidatif Stres Parametreleri Üzerine Etkileri. Gümüşhane Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 6 (1): 41-46.
- Vasylkiv, O.Y., Kubrak, O.I., Storey, K.B., Lushchak, V.I. 2011. Catalase activity as a potential vital biomarker of fish intoxication by the herbicide aminotriazole. Pesticide Biochemistry and Physiology, 101: 1-5.
- Velmurugan Babu, Mariadoss Selvanayagam, Elif Ipek Cengiz, Erhan Unlu. 2009. Histopathological changes in the gill and liver tissues of freshwater fish, Cirrhinus mrigala exposed to dichlorvos. Braz. arch. biol. technol. 52 (5): 1291-1296.
- World Health Organization (WHO) 2016. http://www.who.int/foodsafety/areas_work/chemicalrisks/jmpr/en/ (Erişim: Mart 2021).
- Xing, H., Li, S., Wang, Z., Gao, X., Xu, S. , Wang, X. 2012. Oxidative stress responce and histopathological changes due to atrazine and chlorpyrifos exposure in common carp. Pesticide Biochemistry and Physiology, 103: 74-80.
- Yan, S.H., Wang, J.H., Zhu, L.S., Chen, A.M., Wang, J. 2016. Thiamethoxam induces oxidative stress and antioxidant response in zebrafish (Danio rerio) Livers, Environmental Toxicology, 3: 2006-2015.
- Yan, S.H., Wang, J.H., Zhu, L.S., Chen, A.M., Wang, J. 2015. Toxic effects of nitenpyram on antioxidant enzyme system and DNA in zebrafish (Danio rerio) livers, Ecotoxicology and Environmental Safety, 122: 54–60.
- Yonar, M.E., Sakin, F. 2011. Ameliorative effect of lycopene on antioxidant status in Cyprinus carpio during pyrethroid deltamethrin exposure, Pesticide Biochemistry and Physiology, 99: 226–231.
- Zhang, J.F., Liu, H., Sun, Y.Y., Wang, X.R.,, Wu, J.C., Xue, Y.Q. 2005. Responses of the antioxidant defenses of the goldfish Carassius auratus, exposed to 2,4- Dichlorophenol. Environmental Toxicology and Pharmacology, 19: 185-190.
- Zirong, X., Shijun, B. 2007. Effects of waterborne Cd exposure on glutathione metabolism in Nile tilapia (Oreochromis niloticus) liver, Ecotoxicology and Environmental Safety, 67: 89–94.
2,4-Diklorofenoksiasetik Asit’e Maruz Kalan Capoeta umbla Karaciğer Dokusunda Malondialdehit Seviyesi, Süperoksit dismutaz ve Katalaz Aktivitesindeki Değişimlerin İncelenmesi
Öz
Bu çalışmada, Capoeta umbla balıklarına 2,4-diklorofenoksiasetik asit (2,4-D) herbisitinin iki dozu 72 saat uygulanarak, balık karaciğer dokusundaki malondialdehit seviyesi (MDA), süperoksit dismutaz (SOD) ve katalaz (CAT) aktivitesindeki değişimler incelenmeştir. MDA seviyesi, CAT ve SOD aktivitesindeki değişiklikler, spekrofotometrik yöntemlerle tespit edilmiştir. Çalışma sonucunda, herbisitin farklı dozlarına maruz kalan C. umbla karaciğer dokusunda MDA seviyesi, CAT ve SOD aktivitesinde istatistiksel olarak önemli bir artış olduğu belirlenmiştir (p<0,05). 2,4-D herbisitinin balık karaciğer dokusunda oksidan/antioksidan dengesini bozduğu görülmüştür. Bununla beraber, birincil savunma mekanizması olan bu iki antioksidan enzimin, 2,4-D toksik etkisine karşı direnç gösterdiği görülmektedir.
Anahtar Kelimeler
Capoeta umbla antioksidan enzimler karaciğer herbisit oksidan/antioksidan dengesi
Kaynakça
- Adeyemi, J.A., Martins-Junior, A.C., Barbosa, J.F. 2015. Teratogenicity, genotoxicity and oxidative stress in zebrafish embryos (Danio rerio) co-exposed to arsenic and atrazine. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 7(12): 172-173.
- Aebi, H. 1974. Catalase invitro. In: Methods of enzymatic analysis, Ed: Bergmeyer HU, 2nd ed, FL pp:121-126.
- Fırat, Ö., Aytekin, T. 2018. Neonikotinoid insektisit thiamethoxamın Oreochromis niloticus’ta oksidatif stres parametreleri üzerine etkisi. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 20 (2): 224-234.
- Golombieski, J.I., Sutili, F.J., Salbego, J., Seben, D., Gressler, L.T., Arruda da Cunha, J., Gressler, L.T., Zanella, R., Vaucher, R.A., Marchesan, E., Baldisserotto, B. 2016. Imazapyr+imazapic herbicide determines acute toxicity in silver catfish Rhamdia quelen. Ecotoxicology and Environmental Safety, 128: 91-99.
- Gül S., Nur G., Kaya Ö.T. 2005. 2.4-D’nin Siraz Balığındaki (Capoeta capoeta umbla, Heckel 1843) LC50 Değeri. Türk Sucul Yaşam Dergisi, Sayı 4: 245–249.
- Husak, V.V., Mosiichuk, N.M., Maksymiv, I.V., Storey, J.M., Storey, K.B., Lushchak. V.I. 2016. Oxidative stress responses in gills of goldfish, Carassius auratus, exposed to the metribuzin-containing herbicide Sencor. Environmental Toxicology and Pharmacology, 45: 163- 169.
- Koç, N.D., Akbulut, C. 2012. Histological analysis of acute toxicity of 2,4-diclorophenoxyacetic acid in ovary of zebrafish, Animal Cells and Systems, 16: 400-407.
- Ledwozyw, A., Michalak, D., Stepien, A., Kadziolka, A. 1986. The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human atherosclerosis. Clinica Chimica Acta, 155(3): 275-283.
- Oliveira, J.M.M., Galhano, V., Henriques, I., Soares, A.M.V.M., Loureiro, S. 2017. Basagran® induces developmental malformations and changes the bacterial community of zebrafish embryos. Environmental Pollution, 221: 52-63.
- Oruç, E.Ö., Sevgiler, Y., Üner, N. 2004. Tissue-spesific oxidative stress responses in fish exposed to 2,4-D and azinphosmethyl. Comparative Biochemistry and Physiology, 137: 43-51.
- Özkan, H. 2017. Bazi insektisit ve herbisitlerin kitosan ile adsorpsiyonunun incelenmesi. Yüksek Lisans Tezi, Kimya Anabilim Dalı, Fen Bilimleri Enstitüsü, Süleyman Demirel Üniversitesi, Isparta.
- Persch, T.S.P., Weimer, R.N., Freitas, B.S., Oliveira, G.T. 2017. Metabolic parameters and oxidative balance in juvenile Rhamdia quelen exposed to rice paddy herbicides: Roundup®, Primoleo®, and Facet®. Chemosphere, 174: 98-109.
- Piancini, L.D.S., Guiloski, I.C., Silva de Assis, H.C., Cestari, M.M. 2015. Mesotrione herbicide promotes biochemical changes and DNA damage in two fish species. Toxicology Reports, 2: 1157-1163.
- Sun, Y., Oberley, L. W., Li, Y. 1988. A simple method for clinical assay of superoxide dismutase. Clinical Chemistry 34: 497–500.
- Tabassum, H., Ashafaq, M., Khan, J., Shah, Z., Raisuddin, S., Parvez, S. 2016. Short term exposure of pendimethalin induces biochemical and histological perturbations in liver, kidney and gill of freshwater fish. Ecological Indicators, 63: 29-36.
- Toroser, D., Orr, W.C., Sohal, R.S. 2007. Carbonylation of mitochondrial proteins in Drosophila melanogaster during aging, Biochemical and Biophysical Research Communications, 363: 418–424.
- Tutuş, R. 2016. Oreochromis niloticus’un karaciğer dokusundaki antioksidan sistemler ve lipid peroksidasyonu üzerine chlorpyrifos, emamectin benzoate ve abamectin türü pestisitlerin etkileri, Yüksek Lisans Tezi, Adıyaman Üniversitesi, Fen Bilimleri Enstitüsü, Adıyaman.
- Uçar, A., Al-Hamdani, A. H. A., Alak, G., Atamanalp, M., Topal, A., Arslan, H., Parlak, V., Fakioğlu, Ö., Şensurat, T. 2012. Karboksin’ in Gökkuşağı Alabalıkları(Oncorhynus Mykiss)’nda Süperoksitdismutaz Enzim Aktivitesi Üzerine Etkisi. Biyoloji Bilimleri Araştırma Dergisi 5(2): 83-85.
- Uslu, H., Atila, G. , Ersan, Y., Özden, Z. 2016. İsrail Sazanı (Carassius gibelio Bloch, 1782) Balıklarına Uygulanan Cypermethrin’in Oksidatif Stres Parametreleri Üzerine Etkileri. Gümüşhane Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 6 (1): 41-46.
- Vasylkiv, O.Y., Kubrak, O.I., Storey, K.B., Lushchak, V.I. 2011. Catalase activity as a potential vital biomarker of fish intoxication by the herbicide aminotriazole. Pesticide Biochemistry and Physiology, 101: 1-5.
- Velmurugan Babu, Mariadoss Selvanayagam, Elif Ipek Cengiz, Erhan Unlu. 2009. Histopathological changes in the gill and liver tissues of freshwater fish, Cirrhinus mrigala exposed to dichlorvos. Braz. arch. biol. technol. 52 (5): 1291-1296.
- World Health Organization (WHO) 2016. http://www.who.int/foodsafety/areas_work/chemicalrisks/jmpr/en/ (Erişim: Mart 2021).
- Xing, H., Li, S., Wang, Z., Gao, X., Xu, S. , Wang, X. 2012. Oxidative stress responce and histopathological changes due to atrazine and chlorpyrifos exposure in common carp. Pesticide Biochemistry and Physiology, 103: 74-80.
- Yan, S.H., Wang, J.H., Zhu, L.S., Chen, A.M., Wang, J. 2016. Thiamethoxam induces oxidative stress and antioxidant response in zebrafish (Danio rerio) Livers, Environmental Toxicology, 3: 2006-2015.
- Yan, S.H., Wang, J.H., Zhu, L.S., Chen, A.M., Wang, J. 2015. Toxic effects of nitenpyram on antioxidant enzyme system and DNA in zebrafish (Danio rerio) livers, Ecotoxicology and Environmental Safety, 122: 54–60.
- Yonar, M.E., Sakin, F. 2011. Ameliorative effect of lycopene on antioxidant status in Cyprinus carpio during pyrethroid deltamethrin exposure, Pesticide Biochemistry and Physiology, 99: 226–231.
- Zhang, J.F., Liu, H., Sun, Y.Y., Wang, X.R.,, Wu, J.C., Xue, Y.Q. 2005. Responses of the antioxidant defenses of the goldfish Carassius auratus, exposed to 2,4- Dichlorophenol. Environmental Toxicology and Pharmacology, 19: 185-190.
- Zirong, X., Shijun, B. 2007. Effects of waterborne Cd exposure on glutathione metabolism in Nile tilapia (Oreochromis niloticus) liver, Ecotoxicology and Environmental Safety, 67: 89–94.
Toplam 28 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Yayımlanma Tarihi | 22 Ocak 2022 |
| Gönderilme Tarihi | 19 Ekim 2021 |
| Yayımlandığı Sayı | Yıl 2022 Cilt: 9 Sayı: 1 |
