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trnL İntron ve trnL-F Bölgeleri ile Van İlinde Bulunan Orkide (Orchidaceae) Türlerinin Filogenetik İlişkisinin Belirlenmesi

Yıl 2016, Cilt: 21 Sayı: 2, 83 - 91, 30.12.2016

Ayten Dizkırıcı Sinan İşler Oktay Yiğit

Öz

Van ilinde doğal olarak yayılış gösteren Orkide (Orchis) cinsine ait dokuz türün filogenetik ilişkisi kloroplast DNA’sında bulunan trnL intron ve trnL-F IGS bölgeleri kullanılarak belirlenmeye çalışılmıştır. Aynı cinse ait diğer türlerle olan evrimsel ilişkileri de göstermek için farklı türlerin DNA sekansları NCBI veri tabanından alınarak analize dahil edilmiştir. Her bir tür için ikili okuma yapılarak en doğru DNA sekansı alınmış ve bunlar hizalanarak Maximum Likelihood (ML) methodu ile filogenetik ağaçlar çizdirilmiştir. trnL-F bölgesinde varyasyon çok daha az olmasına rağmen elde edilen ağaç topolojileri oldukça benzerdir. Heriki ağaçta da altcinsler (Orchis, Anacamptis ve Neotinea) farklı kladlarda yer almış ve alt seksiyon seviyesinde tür ayrımı yapılabilmiştir. Çalışma sonunda Anacamptis ve Neotinea altcinslerinde bulunan türlerin taksonomik pozisyonunun değiştirilerek cins seviyesine çıkarılması gerektiği sonucuna varılmıştır.

Kaynakça

  • Aceto, S., Caputo, P., Cozzolino, S., Gaudio, L., and Moretti, A., 1999. Phylogeny and evolution of Orchis and allied genera based on ITS DNA variation: morphological gaps and molecular continuity. Molecular Phylogenetics and Evolution 13: 67-76.
  • Aceto, S., Caputo, P., Gaudio, L., Nazzaro, R., and Cozzolino, S., 2000. Molecular approach to the identification and characterization of natural hybrids between Orchis pauciflora and Orchis quadripunctata (Orchidaceae). Botanica Helvetica 110: 31-39.
  • Altundag, E., and Sevgi, E., 2010. Vegetative anatomy of Orchis italica Poiret. (Orchidaceae) in Turkey. XIII OPTIMA Meeting Proceedings, 22-26.
  • Atwood, JT., 1986. The size of the Orchidaceae and the systematic distribution of epiphytic orchids. Selbyana 9: 171-186.
  • Aybeke, M., Sezik, E., and Olgun, G., 2009. Vegetative anatomy of some Ophrys, Orchis and Dactylorhiza (Orchidaceae) taxa in Thrace region of Turkey. Flora 205: 73-89.
  • Bateman, RM., Pridgeon, A.M., and Chase, M.W., 1997. Phylogenetics of subtribe Orchidine (Orchidoideae, Orchidaceae) based on nuclear ITS sequences. 1. Infrageneric relationships and reclassification to achieve monophyly of Orchis sensu stricto. Lindleyana 12: 113-141.
  • Bateman, R.M., Hollingsworth, P.M., Preston, J., Luo, Y.B., Pridgeon, A.M., and Chase, M.W., 2003. Molecular phylogenetics and evolution of Orchidinae and selected Habenariinae (Orchidaceae). Botanical Journal of the Linnean Society 142: 1-40.
  • Cozzolino, S., Aceto, S., Caputo, P., Nazzaro, R., and Gaudio, L., 1998. Phylogenetic relationships in Orchis and some related genera: an approach using chloroplast DNA. Nordic Journal of Botany 18: 79-87.
  • Cozzolino, S., Aceto, S., Caputo, P., Widmer, A., and Dafni, A., 2001. Speciation processes in eastern Mediterranean Orchis s.l. species: molecular evidence and the role of pollination biology. Israel Journal of Plant Sciences 49: 91-103.
  • Doyle, J.J., and Doyle, J.L., 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11-15.
  • Durmuşkahya, C., Akyol, Y., and Özdemir, C., 2015. Ecology, anatomy and morphology of Orchis spitzelii in Turkey. Journal of Environmental Biology 36: 177-184.
  • Durmuskahya, C., Ozdemir, C., Bozdag, B., and Ozturk, M., 2014. Studies on the Morphology, Anatomy and Ecology of Ophrys lutea Cav. Subsp. Minor (Guss.) O. Danesch & E. Danesch ex Golz & H.R. Reinhard (Orchidaceae) in Turkey. Pakistan Journal of Botany 46: 81-88.
  • Felsenstein, J., 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783-791.
  • Güler. N., 2012. Orchis. In: Güner, A., Aslan, S., Ekim, T., Vural, M., and Babac, M.T., (eds) A Checklist of the Flora of Turkey (Vascular Plants). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, İstanbul.
  • Haider, N., Nabulsi, I., and Kamary, Y., 2012. Phylogeny of Orchidaceae species in northwest Syria based on ISSRs. Journal of Plant Biology Research 1(2): 36-50.
  • Hedge, I.C., 1982. In: Davis, P.H., (ed), Flora of Turkey and the East Aegean Islands: Orchis L., vol 7. University of Edinburgh Press, Edinburgh.
  • Özhatay, N., 2000. Europe’s Medicinal and Aromatic Plants: Their Use, Trade and Conservation. A TRAFFIC Network Report, TRAFFIC International: Cambridge, UK.
  • Pridgeon, A.M., Bateman, R.M., Cox, A.V., Hapeman, J.R., and Chase, M.W., 1997. Phylogenetics of subtribe Orchidine (Orchidoideae, Orchidaceae) based on nuclear ITS sequences. 1. Intergeneric relationships and polyphyly of Orchis sensu lato. Lindleyana 12: 89-109.
  • Quentin, P., 2000. Proposition de revision des genres Orchis, Aceras, Anacamptis et Neotinea. Orchidophile 31(143): 179-182.
  • Sevgi, E., Altundag, E., Kara, O., Sevgi, O., Tecimen, H.B., and Bolat, I., 2012. Morphological and Ecological studies on some Orchis (Orchidaceae) taxa of Mediterranean region, Turkey. Journal of Environmental Biology 33: 343-353.
  • Sgarbi, E., and Del, Prete, C., 2005. Histo-anatomical observations on some Orchis species (Orchidaceae) from the eastern Mediterranean. Flora Mediterranea 15: 321-329.
  • Sramko, G., Molnar, V.A., Hawkins, J.A., and Bateman, R.M., 2014. Molecular phylogeny and evolutionary history of the Eurasiatic orchid genus Himantoglossum s.l. (Orchidaceae). Annals of Botany 114: 1609-1626.
  • Taberlet, P., Gielly, L., Pautou, G., Bouvet, J., 1991. Universal primers for amplification of three non-coding regions of chloroplast DNA, Plant Mol. Biol. 17: 1105-1109
  • Tamura, K., and Nei, M., 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10: 512-526.
  • Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S., 2011. MEGA 5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution 28: 2731-2739.

Yıl 2016, Cilt: 21 Sayı: 2, 83 - 91, 30.12.2016

Ayten Dizkırıcı Sinan İşler Oktay Yiğit

Öz

Kaynakça

  • Aceto, S., Caputo, P., Cozzolino, S., Gaudio, L., and Moretti, A., 1999. Phylogeny and evolution of Orchis and allied genera based on ITS DNA variation: morphological gaps and molecular continuity. Molecular Phylogenetics and Evolution 13: 67-76.
  • Aceto, S., Caputo, P., Gaudio, L., Nazzaro, R., and Cozzolino, S., 2000. Molecular approach to the identification and characterization of natural hybrids between Orchis pauciflora and Orchis quadripunctata (Orchidaceae). Botanica Helvetica 110: 31-39.
  • Altundag, E., and Sevgi, E., 2010. Vegetative anatomy of Orchis italica Poiret. (Orchidaceae) in Turkey. XIII OPTIMA Meeting Proceedings, 22-26.
  • Atwood, JT., 1986. The size of the Orchidaceae and the systematic distribution of epiphytic orchids. Selbyana 9: 171-186.
  • Aybeke, M., Sezik, E., and Olgun, G., 2009. Vegetative anatomy of some Ophrys, Orchis and Dactylorhiza (Orchidaceae) taxa in Thrace region of Turkey. Flora 205: 73-89.
  • Bateman, RM., Pridgeon, A.M., and Chase, M.W., 1997. Phylogenetics of subtribe Orchidine (Orchidoideae, Orchidaceae) based on nuclear ITS sequences. 1. Infrageneric relationships and reclassification to achieve monophyly of Orchis sensu stricto. Lindleyana 12: 113-141.
  • Bateman, R.M., Hollingsworth, P.M., Preston, J., Luo, Y.B., Pridgeon, A.M., and Chase, M.W., 2003. Molecular phylogenetics and evolution of Orchidinae and selected Habenariinae (Orchidaceae). Botanical Journal of the Linnean Society 142: 1-40.
  • Cozzolino, S., Aceto, S., Caputo, P., Nazzaro, R., and Gaudio, L., 1998. Phylogenetic relationships in Orchis and some related genera: an approach using chloroplast DNA. Nordic Journal of Botany 18: 79-87.
  • Cozzolino, S., Aceto, S., Caputo, P., Widmer, A., and Dafni, A., 2001. Speciation processes in eastern Mediterranean Orchis s.l. species: molecular evidence and the role of pollination biology. Israel Journal of Plant Sciences 49: 91-103.
  • Doyle, J.J., and Doyle, J.L., 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11-15.
  • Durmuşkahya, C., Akyol, Y., and Özdemir, C., 2015. Ecology, anatomy and morphology of Orchis spitzelii in Turkey. Journal of Environmental Biology 36: 177-184.
  • Durmuskahya, C., Ozdemir, C., Bozdag, B., and Ozturk, M., 2014. Studies on the Morphology, Anatomy and Ecology of Ophrys lutea Cav. Subsp. Minor (Guss.) O. Danesch & E. Danesch ex Golz & H.R. Reinhard (Orchidaceae) in Turkey. Pakistan Journal of Botany 46: 81-88.
  • Felsenstein, J., 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783-791.
  • Güler. N., 2012. Orchis. In: Güner, A., Aslan, S., Ekim, T., Vural, M., and Babac, M.T., (eds) A Checklist of the Flora of Turkey (Vascular Plants). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, İstanbul.
  • Haider, N., Nabulsi, I., and Kamary, Y., 2012. Phylogeny of Orchidaceae species in northwest Syria based on ISSRs. Journal of Plant Biology Research 1(2): 36-50.
  • Hedge, I.C., 1982. In: Davis, P.H., (ed), Flora of Turkey and the East Aegean Islands: Orchis L., vol 7. University of Edinburgh Press, Edinburgh.
  • Özhatay, N., 2000. Europe’s Medicinal and Aromatic Plants: Their Use, Trade and Conservation. A TRAFFIC Network Report, TRAFFIC International: Cambridge, UK.
  • Pridgeon, A.M., Bateman, R.M., Cox, A.V., Hapeman, J.R., and Chase, M.W., 1997. Phylogenetics of subtribe Orchidine (Orchidoideae, Orchidaceae) based on nuclear ITS sequences. 1. Intergeneric relationships and polyphyly of Orchis sensu lato. Lindleyana 12: 89-109.
  • Quentin, P., 2000. Proposition de revision des genres Orchis, Aceras, Anacamptis et Neotinea. Orchidophile 31(143): 179-182.
  • Sevgi, E., Altundag, E., Kara, O., Sevgi, O., Tecimen, H.B., and Bolat, I., 2012. Morphological and Ecological studies on some Orchis (Orchidaceae) taxa of Mediterranean region, Turkey. Journal of Environmental Biology 33: 343-353.
  • Sgarbi, E., and Del, Prete, C., 2005. Histo-anatomical observations on some Orchis species (Orchidaceae) from the eastern Mediterranean. Flora Mediterranea 15: 321-329.
  • Sramko, G., Molnar, V.A., Hawkins, J.A., and Bateman, R.M., 2014. Molecular phylogeny and evolutionary history of the Eurasiatic orchid genus Himantoglossum s.l. (Orchidaceae). Annals of Botany 114: 1609-1626.
  • Taberlet, P., Gielly, L., Pautou, G., Bouvet, J., 1991. Universal primers for amplification of three non-coding regions of chloroplast DNA, Plant Mol. Biol. 17: 1105-1109
  • Tamura, K., and Nei, M., 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10: 512-526.
  • Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S., 2011. MEGA 5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution 28: 2731-2739.
Toplam 25 adet kaynakça vardır.

Ayrıntılar

Bölüm Makaleler
Yazarlar

Ayten Dizkırıcı

Sinan İşler

Oktay Yiğit Bu kişi benim

Yayımlanma Tarihi 30 Aralık 2016
Gönderilme Tarihi 26 Nisan 2016
Yayımlandığı Sayı Yıl 2016 Cilt: 21 Sayı: 2

Kaynak Göster

APA Dizkırıcı, A., İşler, S., & Yiğit, O. (2016). trnL İntron ve trnL-F Bölgeleri ile Van İlinde Bulunan Orkide (Orchidaceae) Türlerinin Filogenetik İlişkisinin Belirlenmesi. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 21(2), 83-91.