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Farklı Propolis Ekstraktlarının Fusarium solani'ye Karşı Antifungal Etkisi

Year 2022, Issue: 35, 82 - 88, 07.05.2022
https://doi.org/10.31590/ejosat.1055865

Abstract

Propolis, bal arıları tarafından oluşturulan doğal kahverengimsi bir yan üründür. Propolis, içerdiği antimikrobiyal bileşenler nedeniyle tarımsal hasara neden olan önemli fungal patojenlere karşı yaygın olarak test edilmiştir. Propolisin ekstraksiyonunda kullanılan seyreltici, propolisin toplam fenolik bileşikleri ve antimikrobiyal özelliklerinde farklılıklara neden olur. Ascomycota şubesinde yer alan Fusarium solani, bezelye, fasulye, patates, kabakgiller ve mısır gibi birçok bitki türü için infeksiyözdür ve önemli ürün kayıplarına neden olur. Çoğu bitkide genellikle çürümeye, solgunluğa ve nekrotik lekelere ve sonunda bitki ölümüne yol açar.
Bu çalışmada, Türkiye'nin Bingöl ilinden toplanan propolisin antifungal etkisi toplam 9 uygulama grubu ile değerlendirilmiştir. Preparatlar ve kritik doz seviyeleri arasındaki farkları belirlemek için 3 farklı preparasyon (Etanol, (Dimetil sülfoksit (DMSO) ve süperkritik sıvı ekstraksiyon yöntemi ile saflaştırılmış saf propolis) 3 farklı konsantrasyonda (100µl, 200µl ve 600µl) uygulanmıştır. ) PDA ortamında.
Çalışmanın istatistiksel sonuçları, saf propolis ekstraktlarının artan dozlarda (600 µl için yaklaşık %30) patojenin misel büyümesine karşı daha etkili olduğunu gösterdi. Ayrıca, propolisin etanol özleri orta düzeyde bir antifungal etki gösterirken (600 µl için yaklaşık %33), DMSO özleri daha az etkiliydi (600 µl için yaklaşık %58). Bu çalışma, F. solani üzerinde CO2 kullanılarak SFE yöntemiyle saflaştırılmış saf propolisin fungisidal aktivitesini gösteren ilk rapordur. Bu çalışmanın sonuçları, gelecekteki çalışmalarda fungal kaynaklı fitopatojenlere karşı ilaç bazlı stratejilerin geliştirilmesine ışık tutabilir.

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Thanks

Emeği geçenlere ve siz dergi ekibine yürekten teşekkür ediyorum.

References

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Inhibition Effect of Different Propolis Extracts against Fusarium solani in vitro

Year 2022, Issue: 35, 82 - 88, 07.05.2022
https://doi.org/10.31590/ejosat.1055865

Abstract

Propolis is a natural brownish by-product created by honey bees. Due to the antimicrobial components it contains, propolis has been widely tested against important fungal pathogens that cause agricultural damage. The diluent used in the extraction of propolis causes differences in the total phenolic compounds and antimicrobial properties of propolis. Fusarium solani, division Ascomycota, is infectious to many cultivated crops such as peas, beans, potatoes, cucurbits, and maize, resulting in significant crop losses. In most plants, it generally leads to rot, wilt and necrotic spots and eventual plant death.
In this study, the antifungal effect of propolis collected from Bingöl province of Turkey was evaluated against, with a total of 9 groups of applications. In order to determine the differences between preparations and the critical dose levels, 3 different preparations (Ethanol, (Dimethyl sulfoxide (DMSO), and pure propolis purified by supercritical fluid extraction method) were applied in 3 different concentrations (100µl, 200µl, and 600µl) in PDA medium.
The statistical results of the study showed that pure extracts of propolis are more effective at increasing doses (around 30% for 600 µl) against the mycelial growth of pathogen. Furthermore, ethanol extracts of propolis showed a moderate antifungal effect (around 33% for 600 µl), while the DMSO extracts were less effective (around 58% for 600 µl). This study is the first report showing the fungicidal activity of pure propolis purified by the SFE method using CO2 on F. solani. The results of this study may shed light on the development of drug-based strategies against fungal-borne phytopathogens in the future studies.

Project Number

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References

  • Abd-El-Kareem, F., Saied, N.M. & El-Mohamedy, R.S.R., 2018. Seed treatment with chitosan and ethanol-extracted propolisfor suppression bean root rot disease under greenhouse conditions. J. Mater. Environ. Sci. 9(8), 2356–2361.
  • Ahmed, S.D., Mohanad, A.K. & Zaid, N.H., 2008. Study antifungal activity of ethanol extract propolis against Fusarium oxysporum fungi. J. Res. Diyala. Humanit. 31, 93–105.
  • AL-Ani, I., Zimmermann, S., Reichling, J. & Wink, M., 2018. Antimicrobial activities of european propolis collected from various geographic origins alone and in combination with antibiotics. Medicines. 5, 2. doi:10.3390/medicines5010002.
  • Alenezi, S., Manal, NA., Natto, J., De., Koning, HP., Igoli, J., Watson, DG., 2018. Chemical Profiling of Papua New Guinea Propolis and Assay its Antiprotozoal Activity. JAN. 35.
  • Al-Waili, N., Al-Ghamdi, A., Ansari, M., Al-Attal, Y. & Salom, K., 2012 Synergistic effects of honey and propolis toward drug multi-resistant Staphylococcus aureus, Escherichia coli and Candida albicans isolates in single and polymicrobial cultures. Int. J. Med. Sci. 9(9), 793-780.
  • Anjum, S.I., Ullah, A., Khan, K.A., Attaullah, M., Khan, H., Ali, H., Bashir, M.A., Tahir, M., Ansari, M.J., Ghramh, H.A., Adgaba, N. & Dash, C.K., 2019. Composition and functional properties of propolis (bee glue): A review. Saudi J. Biol. Sci. 26(7), 1695-1703.
  • Araujo, M.J.A.M., Bosco, S.M.G. & Sforcin, J.M., 2016. Pythium insidiosum: inhibitory effects of propolis and geopropolis on hyphal growth. Braz. J. Microbiol. 47, 863–869.
  • Benjilali, B., Tantaoui, E.A., Ayadi, A. & Ihlal, M., 1984. Method to study antimicrobial effects of essential oils: application to theantifungal activity of six Moroccan essences. J. Food Prot. 47, 748–752.
  • Buchta, V., Černý, J. & Opletalová, V,. 2011. In vitro antifungal activity of propolis samples of Czech and Slovak origin. Cent. Eur. J. Biol. 6(2), 160-166.
  • Curifuta, M., Vidal, J., Sánchez-Venegas, J., Contreras, A., Salazar, L.A. & Alvear, M., 2012. The in vitro antifungal evaluation of a commercial extract of Chilean propolis against six fungi of agricultural importance. Cien. Inv. Agr. 39(2), 347–359.
  • Çakar, G., Saraç Sivrikaya, I., Karakara, E., Güller, A. 2021. Evaluation of the In Vitro Fungicidal Activity of Summer Savory and Lavender Essential Oils Against Fusarium solani. ISPEC Journal of Agr. Sciences. 5(4): 795-805
  • Deans, S.G., Sobada, K.P., 1990. Antimicrobial Properties of Marjoram (Origanum marjorana L.) Volatile Oil. Flavour Fragr. J. 5, 187-190.
  • Dias, L.G., Pereira, A.P. & Estevinho, L.M., 2012. Comparative study of different Portuguese samples of propolis: Pollinic, sensorial, physicochemical, microbiological characterization and antibacterial activity. Food. Chem. Toxicol. 50(12), 4246-4253.
  • Drescher, N., Klein, A.M., Neumann, P., Yañez, O. & Leonhardt, S.D., 2017. Inside honeybee hives: impact of natural propolis on the ectoparasitic mite Varroa destructor and viruses. Insects. 8(1), 5 doi: 10.3390/insects8010015.
  • Er, Y., 2021. In vitro and in vivo antimicrobial activity of propolis extracts against various plant pathogens. J.Plant Dis. Protect. https://doi.org/10.1007/s41348-021-00437-y.
  • Erdoğan, O., Çelik, A., Yıldız, Ş., & Kökten, K. 2014. Pamukta fide kök çürüklüğü etmenlerine karşı bazı bitki ekstrakt ve uçucu yağlarının antifungal etkisi. Türk Tarım ve Doğa Bilimleri Dergisi 1(3): 398-404.
  • Erdoğan, O., Celik, A., & Zeybek, A. 2016. In Vitro Antifungal Activity of Mint, Thyme, Lavender Extracts and Essential Oils on Verticillium dahliae Kleb. Fresenius Environmental Bulletin, 25(11), 4856-4862.
  • Ertürk, Ö., Yavuz, C. & Sıralı, C., 2011. The Antimicrobial Activity of Propolis from Ordu Province of Turkey. HARUM. 28, 11-16. (in Turkish).
  • Ezazi, R., & Davari, M., 2018. Antifungal activity of ethanolic extract of propolis (EEP) against some postharvest fungi. Biological Control of Pests and Plant Diseases. 1(1), 103-107.
  • Falcão, SI., Vale, N., Cos, P., Games, P., Freire,C., Maes, L. & Vilas-Boas, M., 2014. In Vitro Evaluation of Portuguese Propolis and Floral Sources for Antiprotozoal, Antibacterial and Antifungal Activity. Phytother. Res. Res. 28, 437–443.
  • Farnesi, A.P., Aquino-Ferreira, R., De Jong, D. & Bastos, J.K., 2009. Effects of stingless bee and honey bee propolis on four species of bacteria. Genet. Mol. Res. 8, 635-640.
  • Ganesh P., Dwivedi S.K., 2018. Biological and Chemical Management of Fusarium solani Causing Wilt in Eggplants. IJSRR. 7(4), 1914-1925.
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There are 66 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Articles
Authors

Gözdenur Çakar 0000-0002-2359-1312

Işıl Saraç Sivrikaya 0000-0002-5991-2173

Ersin Karakaya 0000-0003-3887-4208

Abdullah Güller 0000-0003-3887-4208

Project Number bulunmamaktadır
Publication Date May 7, 2022
Published in Issue Year 2022 Issue: 35

Cite

APA Çakar, G., Saraç Sivrikaya, I., Karakaya, E., Güller, A. (2022). Inhibition Effect of Different Propolis Extracts against Fusarium solani in vitro. Avrupa Bilim Ve Teknoloji Dergisi(35), 82-88. https://doi.org/10.31590/ejosat.1055865