Abstract
Lysimachia nummularia L. is an important plant that has traditionally
been used in the treatment of various diseases in Europe and Asia. Due to the
valuable bioactive compounds content, this plant has a very important place in
the pharmaceutical field. In this study, the propagation of L. nummularia by tissue culture
techniques was investigated. The nodal explants of L. nummularia were cultured in Murashige and Skoog (MS) nutrient
media containing 6-benzylaminopurine (BAP) alone or in combination with
indole-3-butyric acid (IBA) at different concentrations. Generally, high rates
of shoot regeneration were obtained in both hormone trials. The maximum number
of shoots per explant (12.27) was obtained in the MS nutrient medium containing
1.6 mg L-1 BAP, followed by MS medium containing 0.8 mg L-1
BAP + 0.1 mg L-1 IBA (11). The lowest number of shoots was
determined in MS medium containing 0.05 mg L-1 BAP in both hormone
applications. The number of shoots increased with increasing BAP concentration
in the culture medium. The longest shoots were recorded in MS medium
supplemented with 0.1 mg L-1 BAP (4.6 cm) and 0.1 mg L-1
BAP + 0.1 mg L-1 IBA (4.52 cm). The shortest shoot lengths were
determined in MS medium supplemented with 1.6 mg L-1 BAP. Since the
regenerated shoots in the culture medium sustaine intensive roots, rooting
experiments have not been carried out. The rooted plants were successfully
acclimatized to the aquarium environment. As a result, the best hormone for the
propagation of L. nummularia by
tissue culture techniques was 1.6 mg L-1 BAP. This work may help to
obtain high amounts of this valuable herbal metabolite by allowing multiple
production of L. nummularia with
tissue culture. Thus, it can be contributed to the food and pharmaceutical
sectors.
References
- Baskaran P, Kumari A, Van Staden J 2018. In Vitro Propagation Via Organogenesis and Synthetic Seeds of Urginea altissima (L.f.) Baker: A Threatened Medicinal Plant. 3 Biotech, 8: 18.
- Başaran MS, Adıgüzel N 2001. Bolu, Bartın ve Zonguldak İlleri Fındık Bahçelerinin Florasının Tespiti. Bitki Koruma Bülteni, 41(1-2): 39-66.
- Challam M, Roy B, Tandon V 2010. Effect of Lysimachia ramosa (Primulaceae) on Helminth Parasites: Motility, Mortality and Scanning Electron Microscopic Observations on Surface Topography. Veterinary Parasitology, 169(1-2), 214–218.
- Dias MI, Sousa MJ, Alves RC, Ferreira ICFR 2016. Exploring Plant Tissue Culture to Improve the Production of Phenolic Compounds: A Review. Industrial Crops and Products, 82: 9-22.
- Dogan M 2017. Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Anatolian Journal of Botany, 1(1):4-8.
- Dogan M, Karatas M, Aasim M 2015. An Efficient In Vitro Plantlet Regeneration of Ceratophyllum demersum L., An Important Medicinal Aquatic Plant. Fresenius Environmental Bulletin, 24(10b): 3499-3504.
- Dogan M, Karatas M, Aasim M, 2016. In Vitro Shoot Regeneration From Shoot Tip and Nodal Segment Explants of Pogostemon erectus (Dalzell) Kuntze, A Multipurpose Ornamental Aquatic Plant. Fresenius Environmental Bulletin, 25(11): 4777-4782.
- Ebrahimi M, Mokhtari A, Amirian R 2018. A Highly Efficient Method for Somatic Embryogenesis of Kelussia odorotissima Mozaff., An Endangered Medicinal Plant. Plant Cell, Tissue and Organ Culture, 132: 99-110.
- Hanganu D., Olah NK, Mocan A,Vlase L, Benedec D, Raita O, Toma CC 2016. Comparative Polyphenolic Content and Antioxidant Activities of Two Romanian Lysimachia Species. Revista de Chimie, 67(2): 227-231.
- Hussain A, Qarshi IA, Nazir H, Ullah I 2012. Plant Tissue Culture: Current Status and Opportunities. (Recent Advances in Plant In Vitro Culture, InTech, Croatia, Ed: Leva A, Rinaldi LMR) 1 p.
- Jafari N, Othman RY, Khalid N, 2011. Effect of Benzylaminopurine (BAP) Pulsing on In Vitro Shoot Multiplication of Musa acuminata (banana) cv. Berangan. African Journal of Biotechnology, 10(13): 2446-2450.
- Karataş M, Aasim M 2015. In Vitro Plantlet Regeneratıon from Nodal Segments of Creepıng Jenny (Lysimachia nummularia L.) - a Medicinal Aquatic Plant. Fresenius Environmental Bulletin, 24(4): 1263-1268.
- Karatas M, Dogan M, Emsen B, Aasim M 2015. Determination of In Vitro Free Radical Scavenging Activities of Various Extracts From In Vitro Propagated Ceratophyllum demersum L.. Fresenius Environmental Bulletin, 24(9a): 2946-2952.
- Kodela PG, Jobson RW 2016. Lysimachia nummularia (Primulaceae) Naturalised in New South Wales, Australia. Journal of Plant Systematics, 19: 153-157.
- Kumar V, Moyo M, Staden JV 2017. Somatic Embryogenesis in Hypoxis hemerocallidea: An Important African Medicinal Plant. South African Journal of Botany, 108: 331-336.
- Li HY, Hao Z, Wang X, Huang L, Li JP 2009. Antioxidant Activities of Extracts and Fractions from Lysimachia Foenum-Graecum Hance. Bioresource Technology, 100(2): 970-974.
- Murashige T, Skoog F 1962. A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures. Physiological Plantarum, 15: 473-497.
- Naik DSR, Prasad B, Nemali G, Naik AS 2015. In Vitro Shoot Proliferation From Shoot Tip Explants of Winged Bean (Psophocarpus tetragonolobus). International Journal of Recent Scientific Research, 6(5): 3985-3987.
- Neumann KH, Kumar A, Imani J 2009. Plant Cell and Tissue Culture - A Tool in Biotechnology, Principles and Practice, Springer-Verlag Berlin Heidelberg, Germany, 1 p.
- Pawar SV, Khandagale VG, Jambhale VM, Jadhav AS, Pawar BD 2015. In Vitro Regeneration Studies in Stevia Through Nodal Segment and Shoot Tip. The BioScan, 10(3): 1007-1010.
- Podolak I, Koczurkiewicz P, Galanty A, Michalik M 2013a. Cytotoxic Triterpene Saponins from the Underground Parts of Six Lysimachia L. Species. Biochemical Systematics and Ecology, 47: 116-120.
- Podolak I, Koczurkiewicz P, Michalik M, Galanty A, Zajdel P, Janeczko Z 2013b. A new Cytotoxic Triterpene Saponin from Lysimachia nummularia L.. Carbohydrate Research, 375:16-20.
- Sharma SK, Patil A, Agnihotri AK, Mehrotra S 2018. In Vitro Conservation of Alectra chitrakutensis: a Critically Endangered Root Parasitic Plant of High Medicinal Importance. Acta Physiologiae Plantarum, 40: 29.
- Silué N, Koné T, Soumahoro AB, Koné M 2016. In Vitro Shoot Tip Multiplication of Bambara Groundnut [Vigna subterranea (L.) Verdc.]. Plant Cell Tissue and Organ Culture, 127(3): 603-611.
- Soni V, Bhushan M 2017. Hormonal Control of Morphogenesis In Vitro in Nodal Segments of Tylophora indica. International Journal of Life-Sciences Scientific Research, 3(4): 1250-1252.
- Toth A, Toth G, Kery A 2014. Polyphenol Composition and Antioxidant Capacity of Three Lysimachia species. Natural Product Communications, 9: 1473-1478.
- Toth ER, Vegh K, Alberti A, Beni S, Kery A 2017. Contribution of Individual Flavonoids in Lysimachia species to the Antioxidant Capacity based on HPLC-DPPH Assay. Natural Product Research, 2017:1-4.
- Trivedi DR, Joshi AG 2014. In Vitro Shoot Regeneration of Stereospermum suaveolens DC. Using Cotyledonary Node and Nodal Explants. Plant Tissue Culture and Biotechnology, 24(2): 235‐246.
- Turker AU, Guner B 2013. Efficient Plant Regeneration of Yellow Loosestrife (Lysimachia vulgaris L.), a Medicinal Plant. Acta Biologica Hungarica, 64(2):218-30.
- Yang X, Wang BC, Zhang X, Liu WQ, Qian JZ, Li W, Deng J, Singh GK, Su HJ 2011. Evaluation of Lysimachia christinae Hance Extracts as Anticholecystitis and Cholagogic Agents in Animals, 137: 57- 63.
- Yasukawa K, Ogawa H, Takido M 1990. Two Flavonol Glycosides from Lysimachia nummularia. Phytochemistry, 29(5): 1707-1708. Yılmaz C, Kormaz H 2017. Terme’nin Biyoçeşitlilik ve Doğal Ortam Özellikleri. Serander Yayınları, Trabzon, Türkiye.
- Zheng W, Xu XD, Dai H, Chen, LQ 2009. Direct Regeneration of Plants Derived from In Vitro Cultured Shoot Tips and Leaves of Three Lysimachia species. Scientia Horticulturae 122: 138-141.
- Zimik M, Arumugam N 2017. Induction of Shoot Regeneration in Cotyledon Explants of the Oilseed Crop Sesamum indicum L.. Journal of Genetic Engineering and Biotechnology, 15(2): 303-308.
Abstract
Lysimachia nummularia L. Avrupa ve Asya'da çeşitli hastalıkların
tedavisinde geleneksel olarak kullanılan önemli bir bitkidir. İçerdiği değerli
biyoaktif bileşiklerden dolayı farmasötik alanda oldukça önemli bir yere
sahiptir. Bu çalışmada, L. nummularia'nın doku kültürü
teknikleri ile üretimi araştırılmıştır. L.
nummularia'nın boğum eksplantları farklı konsantrasyonlarda 6-Benzilaminopurin
(BAP)'i tek olarak veya İndol-3-Bütirik Asit (IBA) ile kombinasyonunu içeren
Murashige ve Skoog (MS) besin ortamında kültüre alınmıştır. Genel olarak her
iki hormon denemelerinde yüksek sürgün rejenerasyon yüzdeleri elde edilmiştir.
Maksimum eksplant başına sürgün sayısı (12.27 adet) 1.6 mg L-1
BAP içeren
MS besin ortamında, ardından 0.8 mg L-1 BAP + 0.1 mg L-1
IBA içeren MS besin ortamında (11 adet) elde edilmiştir. En düşük sürgün sayısı
her iki hormon uygulamasında da 0.05 mg L-1 BAP içeren MS besi
ortamında tespit edilmiştir. Kültür ortamında BAP konsantrasyonunun artması ile
sürgün sayısı artış göstermiştir. En uzun sürgünler 0.1 mg L-1 BAP
eklenmiş MS ortamında (4.6 cm) ve 0.1 mg L-1 BAP + 0.1 mg L-1
IBA eklenmiş MS ortamında (4.52 cm) kaydedilmiştir. En kısa sürgün uzunlukları
1.6 mg L-1 BAP eklenmiş MS ortamında tespit edilmiştir. Kültür
ortamlarındaki rejenere sürgünler yoğun kök oluşturdukları için ayrıca
köklendirme çalışması yapılmamıştır. Köklü bitkiler başarılı şekilde akvaryum
ortamına alıştırılmıştır. Sonuç olarak L. nummularia'nın doku kültürü
teknikleri ile üretimi için en iyi hormon 1.6 mg L-1 BAP olarak
tespit edilmiştir. Bu çalışma, L.
nummularia'nın doku kültürü ile çoklu üretimine imkan sağlayarak, bu
bitkiden değerli bitkisel metabolitlerin yüksek miktarlarda elde edilmesine
yardımcı olabilir. Böylece gıda ve farmasötik sektörlere katkı sunulabilir.
References
- Baskaran P, Kumari A, Van Staden J 2018. In Vitro Propagation Via Organogenesis and Synthetic Seeds of Urginea altissima (L.f.) Baker: A Threatened Medicinal Plant. 3 Biotech, 8: 18.
- Başaran MS, Adıgüzel N 2001. Bolu, Bartın ve Zonguldak İlleri Fındık Bahçelerinin Florasının Tespiti. Bitki Koruma Bülteni, 41(1-2): 39-66.
- Challam M, Roy B, Tandon V 2010. Effect of Lysimachia ramosa (Primulaceae) on Helminth Parasites: Motility, Mortality and Scanning Electron Microscopic Observations on Surface Topography. Veterinary Parasitology, 169(1-2), 214–218.
- Dias MI, Sousa MJ, Alves RC, Ferreira ICFR 2016. Exploring Plant Tissue Culture to Improve the Production of Phenolic Compounds: A Review. Industrial Crops and Products, 82: 9-22.
- Dogan M 2017. Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Anatolian Journal of Botany, 1(1):4-8.
- Dogan M, Karatas M, Aasim M 2015. An Efficient In Vitro Plantlet Regeneration of Ceratophyllum demersum L., An Important Medicinal Aquatic Plant. Fresenius Environmental Bulletin, 24(10b): 3499-3504.
- Dogan M, Karatas M, Aasim M, 2016. In Vitro Shoot Regeneration From Shoot Tip and Nodal Segment Explants of Pogostemon erectus (Dalzell) Kuntze, A Multipurpose Ornamental Aquatic Plant. Fresenius Environmental Bulletin, 25(11): 4777-4782.
- Ebrahimi M, Mokhtari A, Amirian R 2018. A Highly Efficient Method for Somatic Embryogenesis of Kelussia odorotissima Mozaff., An Endangered Medicinal Plant. Plant Cell, Tissue and Organ Culture, 132: 99-110.
- Hanganu D., Olah NK, Mocan A,Vlase L, Benedec D, Raita O, Toma CC 2016. Comparative Polyphenolic Content and Antioxidant Activities of Two Romanian Lysimachia Species. Revista de Chimie, 67(2): 227-231.
- Hussain A, Qarshi IA, Nazir H, Ullah I 2012. Plant Tissue Culture: Current Status and Opportunities. (Recent Advances in Plant In Vitro Culture, InTech, Croatia, Ed: Leva A, Rinaldi LMR) 1 p.
- Jafari N, Othman RY, Khalid N, 2011. Effect of Benzylaminopurine (BAP) Pulsing on In Vitro Shoot Multiplication of Musa acuminata (banana) cv. Berangan. African Journal of Biotechnology, 10(13): 2446-2450.
- Karataş M, Aasim M 2015. In Vitro Plantlet Regeneratıon from Nodal Segments of Creepıng Jenny (Lysimachia nummularia L.) - a Medicinal Aquatic Plant. Fresenius Environmental Bulletin, 24(4): 1263-1268.
- Karatas M, Dogan M, Emsen B, Aasim M 2015. Determination of In Vitro Free Radical Scavenging Activities of Various Extracts From In Vitro Propagated Ceratophyllum demersum L.. Fresenius Environmental Bulletin, 24(9a): 2946-2952.
- Kodela PG, Jobson RW 2016. Lysimachia nummularia (Primulaceae) Naturalised in New South Wales, Australia. Journal of Plant Systematics, 19: 153-157.
- Kumar V, Moyo M, Staden JV 2017. Somatic Embryogenesis in Hypoxis hemerocallidea: An Important African Medicinal Plant. South African Journal of Botany, 108: 331-336.
- Li HY, Hao Z, Wang X, Huang L, Li JP 2009. Antioxidant Activities of Extracts and Fractions from Lysimachia Foenum-Graecum Hance. Bioresource Technology, 100(2): 970-974.
- Murashige T, Skoog F 1962. A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures. Physiological Plantarum, 15: 473-497.
- Naik DSR, Prasad B, Nemali G, Naik AS 2015. In Vitro Shoot Proliferation From Shoot Tip Explants of Winged Bean (Psophocarpus tetragonolobus). International Journal of Recent Scientific Research, 6(5): 3985-3987.
- Neumann KH, Kumar A, Imani J 2009. Plant Cell and Tissue Culture - A Tool in Biotechnology, Principles and Practice, Springer-Verlag Berlin Heidelberg, Germany, 1 p.
- Pawar SV, Khandagale VG, Jambhale VM, Jadhav AS, Pawar BD 2015. In Vitro Regeneration Studies in Stevia Through Nodal Segment and Shoot Tip. The BioScan, 10(3): 1007-1010.
- Podolak I, Koczurkiewicz P, Galanty A, Michalik M 2013a. Cytotoxic Triterpene Saponins from the Underground Parts of Six Lysimachia L. Species. Biochemical Systematics and Ecology, 47: 116-120.
- Podolak I, Koczurkiewicz P, Michalik M, Galanty A, Zajdel P, Janeczko Z 2013b. A new Cytotoxic Triterpene Saponin from Lysimachia nummularia L.. Carbohydrate Research, 375:16-20.
- Sharma SK, Patil A, Agnihotri AK, Mehrotra S 2018. In Vitro Conservation of Alectra chitrakutensis: a Critically Endangered Root Parasitic Plant of High Medicinal Importance. Acta Physiologiae Plantarum, 40: 29.
- Silué N, Koné T, Soumahoro AB, Koné M 2016. In Vitro Shoot Tip Multiplication of Bambara Groundnut [Vigna subterranea (L.) Verdc.]. Plant Cell Tissue and Organ Culture, 127(3): 603-611.
- Soni V, Bhushan M 2017. Hormonal Control of Morphogenesis In Vitro in Nodal Segments of Tylophora indica. International Journal of Life-Sciences Scientific Research, 3(4): 1250-1252.
- Toth A, Toth G, Kery A 2014. Polyphenol Composition and Antioxidant Capacity of Three Lysimachia species. Natural Product Communications, 9: 1473-1478.
- Toth ER, Vegh K, Alberti A, Beni S, Kery A 2017. Contribution of Individual Flavonoids in Lysimachia species to the Antioxidant Capacity based on HPLC-DPPH Assay. Natural Product Research, 2017:1-4.
- Trivedi DR, Joshi AG 2014. In Vitro Shoot Regeneration of Stereospermum suaveolens DC. Using Cotyledonary Node and Nodal Explants. Plant Tissue Culture and Biotechnology, 24(2): 235‐246.
- Turker AU, Guner B 2013. Efficient Plant Regeneration of Yellow Loosestrife (Lysimachia vulgaris L.), a Medicinal Plant. Acta Biologica Hungarica, 64(2):218-30.
- Yang X, Wang BC, Zhang X, Liu WQ, Qian JZ, Li W, Deng J, Singh GK, Su HJ 2011. Evaluation of Lysimachia christinae Hance Extracts as Anticholecystitis and Cholagogic Agents in Animals, 137: 57- 63.
- Yasukawa K, Ogawa H, Takido M 1990. Two Flavonol Glycosides from Lysimachia nummularia. Phytochemistry, 29(5): 1707-1708. Yılmaz C, Kormaz H 2017. Terme’nin Biyoçeşitlilik ve Doğal Ortam Özellikleri. Serander Yayınları, Trabzon, Türkiye.
- Zheng W, Xu XD, Dai H, Chen, LQ 2009. Direct Regeneration of Plants Derived from In Vitro Cultured Shoot Tips and Leaves of Three Lysimachia species. Scientia Horticulturae 122: 138-141.
- Zimik M, Arumugam N 2017. Induction of Shoot Regeneration in Cotyledon Explants of the Oilseed Crop Sesamum indicum L.. Journal of Genetic Engineering and Biotechnology, 15(2): 303-308.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Publication Date | November 30, 2018 |
| Submission Date | January 28, 2018 |
| Acceptance Date | June 5, 2018 |
| Published in Issue | Year 2018Volume: 21 Issue: 6 |
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https://doi.org/10.38093/cupmap.1057290
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