Research Article
Cytogenetic Characteristics of Microtus guentheri, Microtus arvalis sensu lato and Microtus majori (Mammalia: Rodentia) From Turkey: Constitutive Heterochromatin Distribution
Abstract
Conventionally stained
and C- banded karyotypes of Guenther’s vole (Microtus guentheri), Major's pine vole (Microtus majori) and Common vole (Microtus arvalis) were studied from Turkey. Diploid chromosome numbers of M.
guentheri, M. arvalis and M. majori were found as 2n=54 and
NFa=52, 2n=46 and NFa=68 and 2n=54 and NFa=56, respectively. All chromosomes of M. guentheri were
pericentromeric C- band. In Microtus
arvalis (obscurus cytotype) and Microtus
majori karyotypes,
autosomal chromosomes were heterochromatin C band positive and negative band. In M. arvalis (obscurus cytotype), sex
chromosome was C band negative. In this study, heterozygote chromosome was not found
in the obtained autosomal chromosome set of M.
arvalis. M. majori has enlarged heterochromatin block from
centromere to telomere on the long arm of X chromosome. Y chromosome was
completely heterochromatin.
Thanks
This study was conducted after permission was obtained from the Ondokuz Mayıs University Local Ethics Committee for Animal Experiments (permission number: B.30.2.ODM.0.20.09.00-050.04-56). The wild animals used in the study were obtained within the scope of ‘National Biological Variation Inventory and Monitoring Project (UBENIS)’ conducted by the Ministry of Forestry and Water Management. We would like to thank the employees in the Ministry of Forestry and Water Management Kars branch Office for their contribution in the field studies.
References
- Arslan A, Zima J 2014. Karyotypes of the mammals of Turkey and neighbouring regions: review. Folia Zoologica 63: 1-62.
- Arslan A, Toyran K, Gözütok S, Yorulmaz Y, Zima J 2016. Comparison of the chromosome banding patterns in three species of social voles (Microtus irani karamani, M. schidlovskii, M. anatolicus) from Turkey. Turkish journal of Zoology, 40: 910-916.
- Baskevich MI 1996. On the karyological differentiation in Caucasian populations of common vole (Rodentia, Cricetidae, Microtus). Zoologicheskiĭ zhurnal. 75: 297–308. (in Russian with English summary).
- Baskevich MI, Potapov SG, Khlyap LA, Okulova NM, Ashibokov UM, Grigoriev MP, Dzagurova TK 2015. Chromosomal and molecular investigations of cryptic species of the subgenus Terrıcola (Rodentıa, Arvicolinae, Microtus) in the Caucasian region: Analysis of new records. Zoologicheskiĭ zhurnal. 94 (8): 963-971.
- Baskevich MI, Mironova TA, Cherepanova EV, Krivonogov DM 2016. New Data on Chromosomal Variability, Distribution of Sibling Species, and Hybridization of 46-Chromosomal Forms of Microtus arvalis sensu lato (Rodentia, Arvicolinae) in the Upper Volga Basin. Biology Bulletin, 43 (9): 1281–1291.
- Baydemir AN, Albayrak I, Gözütok S 2011. Cytogenetic study on Microtus guentheri (Danford & Alston, 1880) (Mammalia: Rodentia) from Turkey: constitutive heterochromatin distribution and nucleolar organizer regions. Folia Biologica (Kraków), 59: 35–40.
- Çolak E, Yiğit N, Sözen M, Özkurt Ş 1997a. Distribution and taxonomic status of the genus Microtus (Mammalia: Rodentia) in Southeastern Turkey. Israel Journal of Zoology, 43: 391-396.
- Çolak E, Yigit N, Sözen M, Özkurt Ş 1997b. A study on taxonomic status of Microtus subterraneus (de Selys Longchamps, 1836) and Microtus majori Thomas, 1906 (Mammalia: Rodentia) in Turkey. Turkish Journal of Zoology, 22: 119–129 (in Turkish with English summary).
- Çolak E, Sözen M, Yiğit N, Özkurt Ş 1998. A study on ecology and biology of Microtus guentheri Danford and Alston, 1880 (Mammalia: Rodentia) in Turkey. Turkish Journal of Zoology, 22: 289-295.
- Demirtaş S. Gürler AT 2019. New records of the Iranian Vole, Microtus irani Thomas, 1921, from eastern Turkey (Mammalia: Rodentia). Zoology in the Middle East, 65: 91-94.
- Ford CE, Hamerton JL 1956. ‘A colchicine-hypotonic-citrate’ squash sequence for mammalian chromosomes. Stain Technology, 31: 247-251.
- Gileva EA, Rakitin SB 2006. Factors of Maintaining Chromosome Polymorphism in Common Vole Microtus arvalis Pallas, 1779: Reduced Fertility and Meiotic Drive. Russian Journal of Genetics, 42 (5): 498–504.
- Jaarola M, Martínková N, Gündüz I, Brunhoff C, Zima J, Nadachowski A, Amori G, Bulatova NS, Chondropoulos B, Fraguedakis-Tsolis S, González-Esteban J, López-Fuster MJ, Kandaurov AS, Kefelioğlu H, Mathias ML, Villate I, Searle JB 2004. Molecular phylogeny of the speciose vole genus Microtus (Arvicolinae, Rodentia) inferred from mitochondrial DNA sequences. Molecular Phylogenetics and Evolution, 33: 647-663.
- Kefelioğlu H 1995. The taxonomy of the genus of Microtus (Mammalia: Rodentia) and its distribution in Turkey. Turkish Journal of Zoology, 19: 35–63. (in Turkish with English summary).
- Kefelioğlu H, Kryštufek B 1999. The taxonomy of Microtus socialis group (Rodentia: Microtinae) in Turkey, with the description of a new species. Journal of Natural History, 33: 47–67.
- Kozlovskii AI, Bulatova NS, Novikov AD 1988. Double effect of an inversion in the karyotype of common vole. Doklady AN SSSR 298: 994–997 (in Russian).
- Kryštufek B, Vohralik V 2005. Mammals of Turkey and Cyprus. Rodentia I: Sciuridae, Dipodidae, Gliridae, Arvicolinae. Založba Annales, Koper, Slovenia, 292 pp.
- Kryštufek B, Zorenko T, Buzan VE 2012. New insights into the taxonomy and phylogeny of social voles inferred from mitochondrial cytochrome b sequences. Mammalian Biology, 77: 178-182.
- Kuliev G, Bickham J 2010. Karyological Relationships and Biodiversity of the Pine Voles of Azerbaijan: Differentiation of Species from the Greater and Lesser Caucasus Mountains. Occasional Papers, Museum of Texas Tech University, 291 pp.
- Lemskaya NA, Romanenko SA, Golenishchev FN, Rubtsova NV, Sablina OV, Serdukova NA., O’Brien PCM, Fu B, Yiğit N, Ferguson-Smith MA, Yang F, Graphodatsky AS 2010. Chromosomal evolution of Arvicolinae (Cricetidae, Rodentia). III. Karyotype relationships of ten Microtus species. Chromosome Research, 18: 459–471.
- Macholán M, Filippucci MG, Zima J 2001. Genetic variation and zoogeography of pine voles of the Microtus subterraneus / majori group in Europe and Asia Minor. Journal of Zoology, London, 255: 3142.
- Meyer MN, Golenishchev FN, Radjably SI, Sablina OV 1996. Voles (subgenus Microtus Schrank) of Russia and adjacent territories. Proceedings of the Zoological Institute, Russian Academy of Sciences 232: 1–330 (in Russian).
- Modi WS 1987. Phylogenetic analyses of chromosomal banding patterns among the Neartic Arvicolidae (Mammalia: Rodentia). Systematic Zoology, 36: 109–136.
- Musser GG, Carleton MD 1993. Mammal Species of the World: A Taxonomic and Geographic Reference. Smithsonian Institution Press, Washington, 510-756 pp. O’Brien SJ, Menninger JC, Nash WG 2006. Atlas of mammalian chromosomes. Wiley, Hoboken, 714 pp.
- Selçuk AY, Kefelioğlu H 2018. Cytogenetic characteristic of East European vole Microtus levis and common pine vole Microtus subterraneus (Mammalia: Rodentia) from Turkey: Constitutive Heterochromatin Distribution. Biharean Biologist, 12: 13-16.
- Shenbrot GI, Krasnov BR 2005. Atlas of the Geographic Distribution of the Arvicoline Rodents of the World (Rodentia, Muridae: Arvicolinae), Sofia: Pensoft.
- Summer AT 1972. A simple technique for demonstrating centromeric heterochromatin. Experimental Cell Research, 75: 304-306.
- Tougard C, Montuıre S, Volobouev V, Markova E, Contet J, Anıskın V, Quere J 2013. Exploring phylogeography and species limits in the Altai vole (Rodentia: Cricetidae). Biological Journal of the Linnean Society, 108: 434–452.
- Yiğit N, Çolak E 2002. On the distribution and taxonomic status of Microtus guentheri (Danford and Alston, 1880) and Microtus lydius Blackler, 1916 (Mammalia: Rodentia) in Turkey. Turkish Journal Zoology, 26: 197-204.
- Yorulmaz T, Zima J, Arslan A, Kankılıç T 2013. Variations in the C-heterochromatin and AgNOR distribution in the common vole (Microtus arvalis sensu lato) (Mammalia: Rodentia). Archives of Biological Sciences, Belgrade, 65: 989–995.
- Zagorodnyuk IV 1990. Variability and systematics of the Arvicolini (Rodentia). Communication 1. Species and chromosomal numbers. Vestnik Zoologii, 2: 26-37.
- Zima J, Král B 1984. Karyotypes of European mammals I. Institute of Landscape Ecology, 18 (7): 1–51.
- Zima J, Arslan A, Benda P, Macholán M, Kryštufek B 2013. Chromosomal variation in social voles: a Robertsonian fusion in the Günther’s vole. Acta Theriologica, 58: 255–266.
Microtus guentheri, Microtus arvalis ve Microtus majori (Mammalia: Rodentia) Türlerinin Sitogenetik Özellikleri: Konstitütif Heterokromatin Dağılımı
Abstract
Bu çalışmada, Güentheri tarla faresi (Microtus guentheri), Kısa kulaklı kır faresi (Microtus majori) ve Yaygın tarla faresi (Microtus arvalis) türlerinin standart karyotipleri ve kromozomların
C-bant özellikleri belirlendi. M.
guentheri türünün diploid kromozom sayısı (2n) = 54 ve otozomal
kromozomların kol sayısı (NFa) = 52, M.
arvalis (obscurus sitotip)
türünün 2n = 46 ve NFa = 68, M.majori
türünün 2n = 54 ve NFa = 56 şeklindedir. M.
guentheri otozomal ve eşey kromozomlarda pericentromerik C-bant olduğu
belirlendi. Microtus arvalis (obscurus sitotip) ve Microtus majori karyotiplerinde otozomal
kromozomlar C bant pozitif ve negatif şeklindedir. M. arvalis türünde X ve Y kromozomu C bant negatif özelliktedir. M. majori karyotipinde X kromozomunun
uzun kolunda sentromerden telomere doğru genişlemiş heterokromatin blok
bulunmaktadır. Y kromozomu ise tamamen heterokromatindir.
Keywords
C-bantlama Guenther tarla faresi Kısa kulaklı kır faresi Obscurus sitotip Yaygın tarla faresi
References
- Arslan A, Zima J 2014. Karyotypes of the mammals of Turkey and neighbouring regions: review. Folia Zoologica 63: 1-62.
- Arslan A, Toyran K, Gözütok S, Yorulmaz Y, Zima J 2016. Comparison of the chromosome banding patterns in three species of social voles (Microtus irani karamani, M. schidlovskii, M. anatolicus) from Turkey. Turkish journal of Zoology, 40: 910-916.
- Baskevich MI 1996. On the karyological differentiation in Caucasian populations of common vole (Rodentia, Cricetidae, Microtus). Zoologicheskiĭ zhurnal. 75: 297–308. (in Russian with English summary).
- Baskevich MI, Potapov SG, Khlyap LA, Okulova NM, Ashibokov UM, Grigoriev MP, Dzagurova TK 2015. Chromosomal and molecular investigations of cryptic species of the subgenus Terrıcola (Rodentıa, Arvicolinae, Microtus) in the Caucasian region: Analysis of new records. Zoologicheskiĭ zhurnal. 94 (8): 963-971.
- Baskevich MI, Mironova TA, Cherepanova EV, Krivonogov DM 2016. New Data on Chromosomal Variability, Distribution of Sibling Species, and Hybridization of 46-Chromosomal Forms of Microtus arvalis sensu lato (Rodentia, Arvicolinae) in the Upper Volga Basin. Biology Bulletin, 43 (9): 1281–1291.
- Baydemir AN, Albayrak I, Gözütok S 2011. Cytogenetic study on Microtus guentheri (Danford & Alston, 1880) (Mammalia: Rodentia) from Turkey: constitutive heterochromatin distribution and nucleolar organizer regions. Folia Biologica (Kraków), 59: 35–40.
- Çolak E, Yiğit N, Sözen M, Özkurt Ş 1997a. Distribution and taxonomic status of the genus Microtus (Mammalia: Rodentia) in Southeastern Turkey. Israel Journal of Zoology, 43: 391-396.
- Çolak E, Yigit N, Sözen M, Özkurt Ş 1997b. A study on taxonomic status of Microtus subterraneus (de Selys Longchamps, 1836) and Microtus majori Thomas, 1906 (Mammalia: Rodentia) in Turkey. Turkish Journal of Zoology, 22: 119–129 (in Turkish with English summary).
- Çolak E, Sözen M, Yiğit N, Özkurt Ş 1998. A study on ecology and biology of Microtus guentheri Danford and Alston, 1880 (Mammalia: Rodentia) in Turkey. Turkish Journal of Zoology, 22: 289-295.
- Demirtaş S. Gürler AT 2019. New records of the Iranian Vole, Microtus irani Thomas, 1921, from eastern Turkey (Mammalia: Rodentia). Zoology in the Middle East, 65: 91-94.
- Ford CE, Hamerton JL 1956. ‘A colchicine-hypotonic-citrate’ squash sequence for mammalian chromosomes. Stain Technology, 31: 247-251.
- Gileva EA, Rakitin SB 2006. Factors of Maintaining Chromosome Polymorphism in Common Vole Microtus arvalis Pallas, 1779: Reduced Fertility and Meiotic Drive. Russian Journal of Genetics, 42 (5): 498–504.
- Jaarola M, Martínková N, Gündüz I, Brunhoff C, Zima J, Nadachowski A, Amori G, Bulatova NS, Chondropoulos B, Fraguedakis-Tsolis S, González-Esteban J, López-Fuster MJ, Kandaurov AS, Kefelioğlu H, Mathias ML, Villate I, Searle JB 2004. Molecular phylogeny of the speciose vole genus Microtus (Arvicolinae, Rodentia) inferred from mitochondrial DNA sequences. Molecular Phylogenetics and Evolution, 33: 647-663.
- Kefelioğlu H 1995. The taxonomy of the genus of Microtus (Mammalia: Rodentia) and its distribution in Turkey. Turkish Journal of Zoology, 19: 35–63. (in Turkish with English summary).
- Kefelioğlu H, Kryštufek B 1999. The taxonomy of Microtus socialis group (Rodentia: Microtinae) in Turkey, with the description of a new species. Journal of Natural History, 33: 47–67.
- Kozlovskii AI, Bulatova NS, Novikov AD 1988. Double effect of an inversion in the karyotype of common vole. Doklady AN SSSR 298: 994–997 (in Russian).
- Kryštufek B, Vohralik V 2005. Mammals of Turkey and Cyprus. Rodentia I: Sciuridae, Dipodidae, Gliridae, Arvicolinae. Založba Annales, Koper, Slovenia, 292 pp.
- Kryštufek B, Zorenko T, Buzan VE 2012. New insights into the taxonomy and phylogeny of social voles inferred from mitochondrial cytochrome b sequences. Mammalian Biology, 77: 178-182.
- Kuliev G, Bickham J 2010. Karyological Relationships and Biodiversity of the Pine Voles of Azerbaijan: Differentiation of Species from the Greater and Lesser Caucasus Mountains. Occasional Papers, Museum of Texas Tech University, 291 pp.
- Lemskaya NA, Romanenko SA, Golenishchev FN, Rubtsova NV, Sablina OV, Serdukova NA., O’Brien PCM, Fu B, Yiğit N, Ferguson-Smith MA, Yang F, Graphodatsky AS 2010. Chromosomal evolution of Arvicolinae (Cricetidae, Rodentia). III. Karyotype relationships of ten Microtus species. Chromosome Research, 18: 459–471.
- Macholán M, Filippucci MG, Zima J 2001. Genetic variation and zoogeography of pine voles of the Microtus subterraneus / majori group in Europe and Asia Minor. Journal of Zoology, London, 255: 3142.
- Meyer MN, Golenishchev FN, Radjably SI, Sablina OV 1996. Voles (subgenus Microtus Schrank) of Russia and adjacent territories. Proceedings of the Zoological Institute, Russian Academy of Sciences 232: 1–330 (in Russian).
- Modi WS 1987. Phylogenetic analyses of chromosomal banding patterns among the Neartic Arvicolidae (Mammalia: Rodentia). Systematic Zoology, 36: 109–136.
- Musser GG, Carleton MD 1993. Mammal Species of the World: A Taxonomic and Geographic Reference. Smithsonian Institution Press, Washington, 510-756 pp. O’Brien SJ, Menninger JC, Nash WG 2006. Atlas of mammalian chromosomes. Wiley, Hoboken, 714 pp.
- Selçuk AY, Kefelioğlu H 2018. Cytogenetic characteristic of East European vole Microtus levis and common pine vole Microtus subterraneus (Mammalia: Rodentia) from Turkey: Constitutive Heterochromatin Distribution. Biharean Biologist, 12: 13-16.
- Shenbrot GI, Krasnov BR 2005. Atlas of the Geographic Distribution of the Arvicoline Rodents of the World (Rodentia, Muridae: Arvicolinae), Sofia: Pensoft.
- Summer AT 1972. A simple technique for demonstrating centromeric heterochromatin. Experimental Cell Research, 75: 304-306.
- Tougard C, Montuıre S, Volobouev V, Markova E, Contet J, Anıskın V, Quere J 2013. Exploring phylogeography and species limits in the Altai vole (Rodentia: Cricetidae). Biological Journal of the Linnean Society, 108: 434–452.
- Yiğit N, Çolak E 2002. On the distribution and taxonomic status of Microtus guentheri (Danford and Alston, 1880) and Microtus lydius Blackler, 1916 (Mammalia: Rodentia) in Turkey. Turkish Journal Zoology, 26: 197-204.
- Yorulmaz T, Zima J, Arslan A, Kankılıç T 2013. Variations in the C-heterochromatin and AgNOR distribution in the common vole (Microtus arvalis sensu lato) (Mammalia: Rodentia). Archives of Biological Sciences, Belgrade, 65: 989–995.
- Zagorodnyuk IV 1990. Variability and systematics of the Arvicolini (Rodentia). Communication 1. Species and chromosomal numbers. Vestnik Zoologii, 2: 26-37.
- Zima J, Král B 1984. Karyotypes of European mammals I. Institute of Landscape Ecology, 18 (7): 1–51.
- Zima J, Arslan A, Benda P, Macholán M, Kryštufek B 2013. Chromosomal variation in social voles: a Robertsonian fusion in the Günther’s vole. Acta Theriologica, 58: 255–266.
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Details
| Primary Language | English |
|---|---|
| Subjects | Structural Biology |
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Publication Date | December 31, 2019 |
| Submission Date | March 20, 2019 |
| Acceptance Date | August 5, 2019 |
| Published in Issue | Year 2019Cilt 22 (Ek Sayı 2) |
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