Determination of Antioxidant Activity of Ethanol Extract of Gölevez [(Colocasia esculenta (L.)] Tubers

Mehmet Akyüz

doi:10.18016/ksutarimdoga.vi.589216

Research Article

Determination of Antioxidant Activity of Ethanol Extract of Gölevez [(Colocasia esculenta (L.)] Tubers

Year 2019, Cilt 22 (Ek Sayı 2), 388 - 394, 31.12.2019

Mehmet Akyüz

https://doi.org/10.18016/ksutarimdoga.vi.589216

Cited By: 6

Abstract

In
this study, the antioxidant
activity of the ethanolic extract of Colocasia
esculenta tubers were determined by four different antioxidant tests
including DPPH and ABTS radicals scavenging activities, metal chelating
activity and reducing power. The scavenging effect of extract of C. esculenta tubers and standards on
DPPH radical at the highest concentration (600 µg mL^-1) decreased in
the following order: Vitamin C˃Trolox˃C. esculenta˃BHA and were found as 95.4, 93.6, 83.8 and 78.8 %,
respectively. The scavenging effect of C.
esculenta tuber extract and standards on ABTS radical at the highest
concentration (100 µg mL^-1) decreased in the order: Trolox = BHA˃C.
esculenta and were found as 100, 100, 94.6%, respectively. The metal chelating capacity of extract
of C. esculenta tubers and standards
decreased in the order of C. esculenta˃BHA˃Trolox
at lowest concentration (100 µg mL^-1) and was found to be 78.0,
76.0, 63.5 %, respectively. Reducing power of extract of C. esculenta tubers and standards at the highest concentration (600
µg mL^-1) followed the order: BHA˃Trolox˃C.
esculenta. Total
phenolic compound and flavonoid amounts of C.
esculenta tubers were designated as 2400 mg GAE/kg extract and 2050 mg
QE/kg extract, respectively.

Keywords

Colocasia esculenta, Antioxidant activity, Phenolic content, Radical scavenging

References

Ak T, Gülçin İ 2008. Antioxidant and radical scavenging properties curcumin. Chemico-Biological Interactions, 174: 27-37.
Baydar H 2013. Tıbbi ve Aromatik Bitkiler Bilimi ve Teknolojisi (Genişletilmiş 4. Baskı). Süleyman Demirel Üniversitesi, Ziraat Fakültesi Yayınları No: 51, Isparta, 303 s.
Benslama A, Harrar A 2016. Free radicals scavenging activity and reducing power of two Algerian Sahara medicinal plants extracts. International Journal of Herbal Medicine, 4 (6): 158-161.
Blois MS 1958. Antioxidant determinations by the use of a stable free radical. Nature, 26, 1199-1200.
Brighente IMC, Dias M, Verdi LG, Pizzolatti MG 2007. Antioxidant activity and total phenolic content of some Brazilian Species. Pharmaceutical Biology, 45 (2): 156-161.
Dehpour A.A, Ebrahimzadeh MA, Fazel NS, Mohammad NS 2009. Antioxidant activity of the methanol extract of Ferula assafoetida and its essential oil composition, Grasas Y Aceites, 60 (4): 405-412.
Dinis TCP, Madeira VMC, Almeida LM 1994. Action of phenolic derivates (acetoaminophen, salycilate, and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics, 315: 161-169.
Elmastaş M, Gülçin İ, Beydemir Ş, Küfrevioğlu Öİ, Aboul-Enein, HY 2006. A study on the in vitro antioxidant activity of juniper (Juniperus Communis L.) fruit extracts. Analytical Letters, 39, 47-65.
Ferreira Isabel C.F.R, Baptista P, Vilas-Boas M, Barros L 2007. Free radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: Individual cap and stipe activity. Food Chemistry, 100: 1511-1516.
Fitriana WD, Ersam T, Shimizu K, Fatmawati S 2016. Antioxidant activity of Moringa Oleifera extracts. Indonesian Journal of Chemistry, 16 (3): 297-301.
Garlica EJ, Oldoni TLC, Alencar SM, de Reis A, Loguercio AD, Grande RHM 2012. Antioxidant activity by DPPH assay of potential solutions to be applied on Bleached Teeth. Brazilian Dental Journal, 23(1): 22-27.
Gowri R, Madhavan V 2013. Evaluation of antioxidant activity of ethanolic extract of Sphaeranthus amaranthoides Burm. International Drug Development and Research, 5(4): 320-329.
Gülçin İ 2009. Antioxidant activity of L-adrenaline: A structure-activity insight. Chemico-Biological Interactions, 179: 71-80.
Gülçin İ 2010. Antioxidant properties of resveratrol: A structure-activity insight. Innovative Food Science and Emerging Technologies, 11: 210-218.
Gülçin İ 2012. Antioxidant activity of food constituents: an overview. Archives of Toxicology, 86, 345-391.
Gülçin İ, Elmastaş M, Aboul-Enein HY 2012. Antioxidant activity of cloveoil-A powerful antioxidant source, Arabian Journal of Chemistry, 5: 489-499.
Gülçin İ, Huyut H, Elmastaş M, Aboul-Enein HY 2010. Radical scavenging and antioxidant activity of tannic acid. Arabian Journal of Chemistry, 3: 43-53.
Gülçin İ, Şat İG, Beydemir Ş, Elmastaş M, Küfrevioglu Öİ 2004. Comparison of antioxidant activity of clove (Eugenia caryophylata Thunb) buds and lavender (Lavandula stoechas L.). Food Chemistry 87, 393-400.
Khan N, Al Daghri, NM, Al Ajlan AS, Alokail MS 2014. The use of natural and derived sources of flavonoids and antioxidants in Saudi Arabia. Integrative Food Nutrition Metabolism, 1(2): 100-106.
Li HM, Hwang SH, Kang BG, Hong JS, Lim SS 2014. Inhibitory effects of Colocasia esculenta (L.) Schott constituents on aldose reductase, Molecules, 19: 13212-13224.
Liaudanskas M, Zymone K, Viškelis J, Klevinskas A, Janulis V 2017. Determination of the phenolic composition and antioxidant activity of pear extracts. Hindawi Journal of Chemistry, Article ID, 7856521: 1-9.
Madsen HL, Bertelsen G 1995. Species as antioxidants. Trends in Food Science and Technology, 6 (8): 271-277.
Mates JM 2000. Effects of antioxidant enzymes in the molecular control of reactive oxygen species toxicology. Toxicology, 153: 83–104.
Mathangi T, Prabhakaran, P 2013. DPPH free radical scavenging activity of the extracts of the aquatic fern Marsilea quadrifolia Linn. International Journal of Current Microbiology and Applied Sciences, 2(10): 534-536.
Moreno MIN, Isla MI, Sampietro AR, Vattuone MA 2000. Comparison of the free radical scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacol, 71: 109-114.
Nakharekar VG, Berde CV 2016. Secondary metabolites and nutritional value profiling of Colocasia esculenta. World Journal of Pharmaceutical Research, 5 (10): 709-720.
Oyaizu M 1986. Studies on product of browning reaction prepared from glucosamine. Japan Journal of Nutrition, 44: 307-315.
Park YK, Koo MH, Ikegaki M, Contado, JL 1997. Comparison of the flavonoid aglycone contents of Apis mellifera propolis from various regions of Brazil. Arquivos de Biologia e Technologia 40 (1): 97-106.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine 26: 1231-1237.
Ruiz Ruiz, JC, Moguel Ordoñez, YB, Matus Basto, A, Segura Campos, MR 2014. Antioxidant capacity of leaf extracts from two Stevia rebaudiana Bertoni varieties adapted to cultivation in Mexico. Nutritients Hosp. 31(3), 1163-1170.
Shang HM, Zhou HZ, Yang JY, Li R, Song H, Wu HX 2018. In vitro and in vivo antioxidant activities of inulin. Plos One, 2, 1-12.
Sheikh MA, Tembhre M, 2016. Preliminary phytochemical screening, in vitro antioxidant activity, total phenolic and total flavonoid contents of Colocasia esculenta leaf extract. Asian Journal of Experimental Sciences 30: (1&2): 39-43.
Slinkard K, Singleton VL 1977. Total phenol analyses: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28: 49-55.
Tanker N, Koyuncu M, Coşkun M 2014. Farmasötik Botanik, Ankara Üniversitesi Eczacılık Fakültesi Yayınları No: 105, Ankara, 424s.
Telci İ, Elmastas M, Sahin A 2009. Chemical composition and antioxidant activity of Ocimum minimum essential oils. Chemistry of Natural Compounds, 45 (4): 568-571.
Tuti MD, Pal RS, Arun Kumar R, Bist JK, Bhatt JC 2015. Colocasia based cropping systems affects the antioxidant properties and productivity of colocasia [Colocasia esculenta (l.) schott] tuber. The Bioscan an International Quaterly Journal of Life Sciences, 10 (1): 117-123.
Yadav M, Kushawaha DK, Chatterji S, Watal G 2017. Assessment of antioxidant activity and phytochemical screening of Colocasia esculenta corm. International Journal of Pharmaceutical Sciences and Research, 8(4): 1758-1764.

Gölevez [(Colocasia esculenta (L.)] Yumrularının Etanol Ekstresinin Antioksidan Aktivitesinin Belirlenmesi

Year 2019, Cilt 22 (Ek Sayı 2), 388 - 394, 31.12.2019

Mehmet Akyüz

https://doi.org/10.18016/ksutarimdoga.vi.589216

Cited By: 6

Abstract

Bu çalışmada Gölevez (Colocasia esculenta) yumrularının etanol ekstresinin antioksidan
aktivitesi DPPH ve ABTS radikallerini giderme aktiviteleri, metal şelatlama
aktivitesi ve indirgeme gücü gibi dört farklı antioksidan metotla belirlendi. C.esculenta yumrularının ekstreleri ve
standartların en yüksek konsantrasyonda (600 µg mL^-1) DPPH radikali
üzerindeki giderme etkisi Vitamin C ˃Trolox˃C.esculenta˃BHA
sırasına göre azaldı ve sırasıyla % 95.4, 93.6, 83.8 ve 78.8
olarak bulundu. C. esculenta
yumrularının ekstreleri ve standartların en yüksek konsantrasyonda (100 µg mL^-1)
ABTS radikali üzerindeki giderme etkisi Trolox=BHA˃C.esculenta şeklinde
azaldı ve sırasıyla % 100, 100 ve 94.6 olarak bulundu. C.esculenta yumrularının ekstreleri ve standartların en düşük
konsantrasyonda (100 µg mL^-1) metal şelatlama aktiviteleri C. esculenta˃BHA˃Trolox sırasına göre azaldı ve sırasıyla % 78.0, 76.0 ve 63.5 olarak bulundu. C. esculenta yumrularının ekstreleri ve
standartların en yüksek konsantrasyonda (600 µg mL^-1) indirgeme
güçleri BHA˃Trolox˃C.esculenta şeklinde sıralandı. C.esculenta yumru ekstrelerinin toplam
fenolik bileşik ve flavonoid miktarları sırasıyla 2400 mg GAE/kg ekstre ve 2050
mgQE/kg ekstre olarak belirlendi.

Keywords

Colocasia esculenta, Antioksidan aktivite, Fenolik içerik, Radikal giderme

References

Ak T, Gülçin İ 2008. Antioxidant and radical scavenging properties curcumin. Chemico-Biological Interactions, 174: 27-37.
Baydar H 2013. Tıbbi ve Aromatik Bitkiler Bilimi ve Teknolojisi (Genişletilmiş 4. Baskı). Süleyman Demirel Üniversitesi, Ziraat Fakültesi Yayınları No: 51, Isparta, 303 s.
Benslama A, Harrar A 2016. Free radicals scavenging activity and reducing power of two Algerian Sahara medicinal plants extracts. International Journal of Herbal Medicine, 4 (6): 158-161.
Blois MS 1958. Antioxidant determinations by the use of a stable free radical. Nature, 26, 1199-1200.
Brighente IMC, Dias M, Verdi LG, Pizzolatti MG 2007. Antioxidant activity and total phenolic content of some Brazilian Species. Pharmaceutical Biology, 45 (2): 156-161.
Dehpour A.A, Ebrahimzadeh MA, Fazel NS, Mohammad NS 2009. Antioxidant activity of the methanol extract of Ferula assafoetida and its essential oil composition, Grasas Y Aceites, 60 (4): 405-412.
Dinis TCP, Madeira VMC, Almeida LM 1994. Action of phenolic derivates (acetoaminophen, salycilate, and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics, 315: 161-169.
Elmastaş M, Gülçin İ, Beydemir Ş, Küfrevioğlu Öİ, Aboul-Enein, HY 2006. A study on the in vitro antioxidant activity of juniper (Juniperus Communis L.) fruit extracts. Analytical Letters, 39, 47-65.
Ferreira Isabel C.F.R, Baptista P, Vilas-Boas M, Barros L 2007. Free radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: Individual cap and stipe activity. Food Chemistry, 100: 1511-1516.
Fitriana WD, Ersam T, Shimizu K, Fatmawati S 2016. Antioxidant activity of Moringa Oleifera extracts. Indonesian Journal of Chemistry, 16 (3): 297-301.
Garlica EJ, Oldoni TLC, Alencar SM, de Reis A, Loguercio AD, Grande RHM 2012. Antioxidant activity by DPPH assay of potential solutions to be applied on Bleached Teeth. Brazilian Dental Journal, 23(1): 22-27.
Gowri R, Madhavan V 2013. Evaluation of antioxidant activity of ethanolic extract of Sphaeranthus amaranthoides Burm. International Drug Development and Research, 5(4): 320-329.
Gülçin İ 2009. Antioxidant activity of L-adrenaline: A structure-activity insight. Chemico-Biological Interactions, 179: 71-80.
Gülçin İ 2010. Antioxidant properties of resveratrol: A structure-activity insight. Innovative Food Science and Emerging Technologies, 11: 210-218.
Gülçin İ 2012. Antioxidant activity of food constituents: an overview. Archives of Toxicology, 86, 345-391.
Gülçin İ, Elmastaş M, Aboul-Enein HY 2012. Antioxidant activity of cloveoil-A powerful antioxidant source, Arabian Journal of Chemistry, 5: 489-499.
Gülçin İ, Huyut H, Elmastaş M, Aboul-Enein HY 2010. Radical scavenging and antioxidant activity of tannic acid. Arabian Journal of Chemistry, 3: 43-53.
Gülçin İ, Şat İG, Beydemir Ş, Elmastaş M, Küfrevioglu Öİ 2004. Comparison of antioxidant activity of clove (Eugenia caryophylata Thunb) buds and lavender (Lavandula stoechas L.). Food Chemistry 87, 393-400.
Khan N, Al Daghri, NM, Al Ajlan AS, Alokail MS 2014. The use of natural and derived sources of flavonoids and antioxidants in Saudi Arabia. Integrative Food Nutrition Metabolism, 1(2): 100-106.
Li HM, Hwang SH, Kang BG, Hong JS, Lim SS 2014. Inhibitory effects of Colocasia esculenta (L.) Schott constituents on aldose reductase, Molecules, 19: 13212-13224.
Liaudanskas M, Zymone K, Viškelis J, Klevinskas A, Janulis V 2017. Determination of the phenolic composition and antioxidant activity of pear extracts. Hindawi Journal of Chemistry, Article ID, 7856521: 1-9.
Madsen HL, Bertelsen G 1995. Species as antioxidants. Trends in Food Science and Technology, 6 (8): 271-277.
Mates JM 2000. Effects of antioxidant enzymes in the molecular control of reactive oxygen species toxicology. Toxicology, 153: 83–104.
Mathangi T, Prabhakaran, P 2013. DPPH free radical scavenging activity of the extracts of the aquatic fern Marsilea quadrifolia Linn. International Journal of Current Microbiology and Applied Sciences, 2(10): 534-536.
Moreno MIN, Isla MI, Sampietro AR, Vattuone MA 2000. Comparison of the free radical scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacol, 71: 109-114.
Nakharekar VG, Berde CV 2016. Secondary metabolites and nutritional value profiling of Colocasia esculenta. World Journal of Pharmaceutical Research, 5 (10): 709-720.
Oyaizu M 1986. Studies on product of browning reaction prepared from glucosamine. Japan Journal of Nutrition, 44: 307-315.
Park YK, Koo MH, Ikegaki M, Contado, JL 1997. Comparison of the flavonoid aglycone contents of Apis mellifera propolis from various regions of Brazil. Arquivos de Biologia e Technologia 40 (1): 97-106.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine 26: 1231-1237.
Ruiz Ruiz, JC, Moguel Ordoñez, YB, Matus Basto, A, Segura Campos, MR 2014. Antioxidant capacity of leaf extracts from two Stevia rebaudiana Bertoni varieties adapted to cultivation in Mexico. Nutritients Hosp. 31(3), 1163-1170.
Shang HM, Zhou HZ, Yang JY, Li R, Song H, Wu HX 2018. In vitro and in vivo antioxidant activities of inulin. Plos One, 2, 1-12.
Sheikh MA, Tembhre M, 2016. Preliminary phytochemical screening, in vitro antioxidant activity, total phenolic and total flavonoid contents of Colocasia esculenta leaf extract. Asian Journal of Experimental Sciences 30: (1&2): 39-43.
Slinkard K, Singleton VL 1977. Total phenol analyses: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28: 49-55.
Tanker N, Koyuncu M, Coşkun M 2014. Farmasötik Botanik, Ankara Üniversitesi Eczacılık Fakültesi Yayınları No: 105, Ankara, 424s.
Telci İ, Elmastas M, Sahin A 2009. Chemical composition and antioxidant activity of Ocimum minimum essential oils. Chemistry of Natural Compounds, 45 (4): 568-571.
Tuti MD, Pal RS, Arun Kumar R, Bist JK, Bhatt JC 2015. Colocasia based cropping systems affects the antioxidant properties and productivity of colocasia [Colocasia esculenta (l.) schott] tuber. The Bioscan an International Quaterly Journal of Life Sciences, 10 (1): 117-123.
Yadav M, Kushawaha DK, Chatterji S, Watal G 2017. Assessment of antioxidant activity and phytochemical screening of Colocasia esculenta corm. International Journal of Pharmaceutical Sciences and Research, 8(4): 1758-1764.

There are 37 citations in total.

Details

Primary Language	English
Journal Section	RESEARCH ARTICLE
Authors	Mehmet Akyüz 0000-0001-7971-8389
Publication Date	December 31, 2019
Submission Date	July 9, 2019
Acceptance Date	August 23, 2019
Published in Issue	Year 2019Cilt 22 (Ek Sayı 2)

Cite

APA	Akyüz, M. (2019). Determination of Antioxidant Activity of Ethanol Extract of Gölevez [(Colocasia esculenta (L.)] Tubers. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 22, 388-394. https://doi.org/10.18016/ksutarimdoga.vi.589216