Year 2020, Volume 23 , Issue 4, Pages 1054 - 1063 2020-08-31

Endemik Heliotropium samolifolium subsp. erzurumicum’un Topraküstü ve Toprakaltı Ekstraklarının Biyolojik ve Antioksidan Aktivitelerinin Karşılaştırılması
Comparison of Biological and Antioxidant Activities of Above and Below-Ground Extracts of Endemic Heliotropium samolifolium subsp. erzurumicum

Galip SAĞLAM [1] , Nezahat KANDEMİR [2]


Bu çalışmada, Heliotropium samolifolium subsp. erzurumicum’un topraküstü ve toprakaltı ekstraklarının antioksidan, antimikrobiyal aktiviteleri ve DNA hasarı üzerine etkileri belirlendi ve karşılaştırıldı. Bu alttür Türkiye de sadece Olur (Erzurum) çevresinde yayılış göstermektedir ve halk tarafından Erzurum Bambulu olarak bilinmektedir. Heliotropium L. türleri pirolizidin alkoloitleri, terpenoitler, saponinler, fenoller, flavonoitler taninler ve steroitler gibi sekonder metabolitleri içermektedir. Alttürün topraküstü ve toprakaltı ekstrakları farklı organik çözücüler kullanılarak hazırlandı. Antioksidan çalışmalar için DPPH ve toplam fenolik içerik hesaplama metotları uygulandı. Ekstrakların antimikrobiyal aktivite testleri dört farklı standart suş, bir maya mantarı ve MIK metodu kullanılarak yapıldı. Bitki ekstraklarının DNA hasarı üzerindeki etkileri pBR322 plazmit DNA’sı kullanılarak açıklandı. Bu alttürün toprakaltı etanol ekstraklarının daha güçlü antimikrobiyal aktiviteye sahip olduğu görüldü. Antioksidan sonuçlara göre, en yüksek aktivite topraküstü kloroform, etanol+su ve toprakaltı etanol ekstraklarında bulundu. Ayrıca toprakaltı su ve kloroform ekstrakları pBR322 plazmit DNA’nın açık halkasal formu üzerinde daha büyük bir etkiye sahiptir. Alttürün toprakaltı ekstraklarının topraküstü ekstraklarından daha etkili olduğu belirlendi. Bu alttürden elde edilen ekstrakların ilaç endüstrisinde ve halk hekimliğinde kullanılabileceği önerildi.

In this study, antioxidant, antimicrobial activities and effects on DNA damage of above and below-ground extracts of Heliotropium samolifolium subsp. erzurumicum were determined. This subspecies distributes only in the vicinity of Olur (Erzurum) in Turkey and is known as Erzurum Bambulu by the people. Heliotropium L. species include secondary metabolities such as; pyrrolizidine alkaloids, terpenoids, saponins, phenols, flavonoids, tannis, and steroids. The above and below-ground extracts of the subspecies were prepared using different organic solvents. For the antioxidant studies, DPPH and total phenolic content calculation methods were applied. The antimicrobial activity tests of the extracts were performed using four different standard strains, a yeast and MIC (Minimum Inhibition Concentration) method. The effects on DNA damage of plant extracts were explained using pBR322 plasmid DNA. The below-ground ethanol extract of the subspecies was seen to have stronger antimicrobial activity. According to antioxidant data, the highest activity was found in above-ground ethanol+aqueous, chloroform and below-ground ethanol extracts. Also, the below-ground aqueous and chloroform extracts hada greater effect on the open ring form of pBR322 plasmid DNA. It was determined that the below ground extracts of the subspecies were more effective than the above ground extracts. It was suggested that the extracts obtained from this subspecies may be used in medicine industry and folk medicine.

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Primary Language en
Subjects Biology
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-0844-3127
Author: Galip SAĞLAM
Institution: Amasya Üniversity
Country: Turkey


Orcid: 0000-0002-5428-4139
Author: Nezahat KANDEMİR (Primary Author)
Institution: Amasya University
Country: Turkey


Supporting Institution Amasya University
Project Number FMB-BAP 17-0286
Thanks This study was produced within the scope of the project of FMB-BAP 17-0286 supported by Amasya University. This study is derived from master thesis Galip SAĞLAM's.
Dates

Application Date : January 9, 2020
Acceptance Date : March 13, 2020
Publication Date : August 31, 2020

APA Sağlam, G , Kandemi̇r, N . (2020). Comparison of Biological and Antioxidant Activities of Above and Below-Ground Extracts of Endemic Heliotropium samolifolium subsp. erzurumicum . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (4) , 1054-1063 . DOI: 10.18016/ksutarimdoga.vi.672571