Year 2020, Volume 23 , Issue 5, Pages 1359 - 1365 2020-10-31

The Effects on the Growth Performance, Some Serum Oxidative and Nitrosative Stress Parameters of the Stocking Density in the Sprague-Dawley Rats
Yerleşim Sıklığının Sprague Dawley Ratlarda Büyüme Performansı, Bazı Serum Oksidatif ve Nitrosatif Stres Parametreleri Üzerine Etkisi

Murat GENÇ [1]


Objective of his studywas to compare the growth performances of Sprague-Dawley female rats fed at normal and narrowed stocking density with certain stress parameters. 72 Sprague-Dawley female rats aged 4 weeks were used in the experiment. The average live weight per trial for normal and narrow stock density groups was calculated as 54.22 ± 2.65 and 55.53 ± 2.66 g. Animals’ weekly weighs during a month indicated that as their weight increased, the floor area per animal gradually expanded. Female rats reach sexual maturity at 8-9 weeks. For this reason, the experiment was continued until the animals were 2 months old. Based on the weekly weighing results of the rats, it was found that live weights increased regularly for both groups and growth performances did not change according to stocking density (P>0.05). It was concluded that serum Total Antioxidant Capacity (TAC) and Glutathione (GSH) values were affected by the stocking density and that significantly reduced in the rats fed at narrowed stocking density. On the other hand, Total Oxidant Capacity (TOC), Nitric Oxide (NO) and malondialdehyde (MDA) values increased approximately 1.5 times in the rats at the group of narrowed stocking density. As a result, it was found that even if the increasing stocking density has no negative effect on the growth performance of the animals, it causes some physiological changes in blood values by stressing the animals. Therefore, in order to get the most accurate results in scientific studies, it must be paid attention to breed the animals at the ideal housing conditions.

Bu çalışma, normal ve dar yerleşim sıklığında yetiştirilen dişi Sprague-Dawley ratların büyüme performanslarının ve bazı stres parametrelerinin karşılaştırılması amacıyla yapılmıştır. Denemede 4 haftalık yaştaki 72 adet Sprague Dawley cinsi dişi rat kullanılmıştır. Normal ve dar yerleşim sıklığı grupları için deneme başı canlı ağırlık ortalamaları 54.22±2.65 ve 55.53±2.66 gr olarak hesaplanmıştır. Hayvanlar 1 ay boyunca haftalık olarak tartılmış ve ağırlıkları arttıkça hayvan başına düşen taban alanları tedrici olarak artırılmıştır. Dişi ratlar, 8-9. haftada cinsel olgunluğa eriştiği için, deneme hayvanlar 2 aylık oluncaya kadar sürdürülmüştür. Ratların haftalık tartım sonuçlarına göre canlı ağırlıkların her iki grup için düzenli bir artış gösterdiği ve büyüme performanslarının yerleşim sıklığına göre değişmediği tespit edilmiştir (P>0.05). Serum Toplam Antioksidan Kapasite (TAC) ve Glutatyon (GSH) değerlerinin yerleşim sıklığından etkilendiği ve dar yerleşim sıklığında yetiştirilen ratlarda önemli ölçüde azaldığı sonucuna varılmıştır. Toplam Oksidan Kapasite (TOC), Nitrik oksit (NO) ve Malondialdehit (MDA) değerlerinin ise dar yerleşim sıklığı grubundaki ratlarda yaklaşık 1.5 kat arttığı belirlenmiştir. Sonuç olarak artan stoklama yoğunluğunun hayvanların büyüme performansı üzerine olumsuz bir etkisi olmasa bile, hayvanları strese sokarak kan değerlerinde bir takım fizyolojik değişikliklere yol açtığı saptanmıştır. Bu sebeple bilimsel çalışmalarda en doğru sonucun elde edilebilmesi için hayvanların mutlaka ideal barındırma koşullarında yetiştirilmiş olmasına dikkat edilmelidir.
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Primary Language en
Subjects Basic Sciences
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-9565-0887
Author: Murat GENÇ (Primary Author)
Institution: ATATÜRK ÜNİVERSİTESİ
Country: Turkey


Thanks The author acknowledges Prof. Dr. Fatih Mehmet KANDEMIR for biochemical analysis.
Dates

Application Date : January 28, 2020
Acceptance Date : March 14, 2020
Publication Date : October 31, 2020

APA Genç, M . (2020). The Effects on the Growth Performance, Some Serum Oxidative and Nitrosative Stress Parameters of the Stocking Density in the Sprague-Dawley Rats . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (5) , 1359-1365 . DOI: 10.18016/ksutarimdoga.vi.681294