Evaluation of Antioxidant and Antimicrobial Activities of Potentilla recta L.

Gökçe Şeker Karatoprak; Başak Ökdem; Selen İlgün; Muberra Kosar

doi:10.18016/ksutarimdoga.vi.894015

EN TR

Evaluation of Antioxidant and Antimicrobial Activities of Potentilla recta L.

Abstract

In this study, the antioxidant activity and effectiveness against pathogenic microorganisms in the skin were investigated to evaluate the wound healing mechanisms of Potentilla recta L. (Rosaceae), which is traditionally used in wound treatment. The antioxidant activity was determined using 1,1-Diphenyl-2-picrylhydrazyl (DPPH•) and2,2-Azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS•+) radical scavenging activities, inhibition of β-carotene bleaching, protection of 2-deoxyribose and bovine brain-derived phospholipids against hydroxyl radical-mediated degradation assay, at different concentrations ranging from 0.001 to 2 mg mL-1. By the microdilution method, antimicrobial activity was evaluated on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Candida albicans. Besides, viability enhancing effects on murine fibroblast cells (L929) were determined by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method. The key parameters for the extract included the following: DPPH• IC50: 0.19 mg mL-1, TEAC: 0.76 mmol L-1 Trolox, reduction: 0.87 mmol g-1 AsscE, and protection of lipid peroxidation IC50: 0.07 mg mL-1. A strong effect on S. epidermidis was observed with 79% inhibition at a concentration of 125 mg mL-1, but did not show toxicity to L929 cells below 250 mg mL-1 concentration. The results proved that the potent antioxidant activity combined with the antimicrobial activity supports traditional use.

Keywords

Potentilla recta L.'nin Antioksidan ve Antimikrobiyal Aktivitelerinin Değerlendirilmesi

Öz

Bu çalışmada geleneksel olarak yara tedavisinde kullanılan Potentilla recta L. (Rosaceae) 'nin yara iyileştirme mekanizmalarını değerlendirmek için, ciltteki patojen mikroorganizmalara karşı etkinliği ve antioksidan aktivitesi araştırılmıştır. Antioksidan aktivite 1,1-Difenil-2-pikrilhidrazil (DPPH•) ve 2,2-Azinobis (3-etilbenzotiyazolin-6-sülfonat) (ABTS•+) radikal temizleme, β-karoten ağartmanın inhibisyonu, 2-deoksiriboz ve sığır beyninden türetilmiş fosfolipidlerin hidroksil radikal aracılı degradasyonuna karşı koruma etkileri deneyleri ile 0.001 - 2 mg mL-1 arasında değişen farklı konsantrasyonlarda belirlenmiştir. Antimikrobiyal aktivite, mikrodilüsyon yöntemi ile Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa ve Candida albicans üzerinde değerlendirilmiştir. Ayrıca fare fibroblast hücreleri (L929) üzerindeki canlılığı artırıcı etkiler MTT (3- (4,5-dimetiltiyazol-2-il)-2,5-difeniltetrazolyum bromür) yöntemi ile değerlendirilmiştir. Ekstreler için temel parametreler: DPPH • IC50: 0.19 mg mL-1, TEAC: 0.76 mmol L-1 Trolox, redüksiyon: 0.87 mmol g-1 AsscE ve lipid peroksidasyonun korunması IC50: 0.07 mg mL-1 olarak belirlenmiştir. 125 mg mL-1 konsantrasyonda % 79 inhibisyonla S. epidermidis üzerinde güçlü bir etki gözlenirken 250 mg mL-1 konsantrasyonunun altında L929 hücrelerine toksisite gözlenmemiştir. Sonuçlar, antimikrobiyal aktivite ile birlikte güçlü antioksidan aktivitenin geleneksel kullanımı desteklediğini kanıtlamıştır.

Anahtar Kelimeler

Supporting Institution

Erciyes Üniversitesi

Project Number

TCD-2015-5609

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Gökçe Şeker Karatoprak ^*
0000-0001-5829-6914
Türkiye

Başak Ökdem
0000-0003-1482-5938
Türkiye

Selen İlgün
0000-0002-8544-0683
Türkiye

Muberra Kosar
0000-0002-1559-998X
Türkiye

Publication Date

June 30, 2022

Submission Date

March 10, 2021

Acceptance Date

July 1, 2021

Published in Issue

Year 2022 Volume: 25 Number: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.894015

IZ

https://izlik.org/JA23HF53SS

Cite

RIS / Bibtex

APA

Şeker Karatoprak, G., Ökdem, B., İlgün, S., & Kosar, M. (2022). Evaluation of Antioxidant and Antimicrobial Activities of Potentilla recta L. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(3), 439-448. https://doi.org/10.18016/ksutarimdoga.vi.894015

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