Research Article

Determination of Total Antioxidant Capacity, Radical Scavenging Activity and Fatty Acid Composition of Ultrasonic Assisted Ethanolic Extraction of Oat (Avena sativa L.) Grains

Volume: 25 Number: Ek Sayı 2 December 30, 2022
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Determination of Total Antioxidant Capacity, Radical Scavenging Activity and Fatty Acid Composition of Ultrasonic Assisted Ethanolic Extraction of Oat (Avena sativa L.) Grains

Abstract

In this study, it was aimed to determine the total antioxidant capacity and free radical scavenging activity of the extracts obtained by ultrasonic assisted extraction of oat grains, as well as to determine the fatty acid profile and total tocopherol content of oat extracts. For this purpose, we determined the total antioxidant capacity of oat grains extracts according to the copper ions (Cu2+) reducing antioxidant capacity (CUPRAC) test. At the same time, the radical scavenging capacity of oat extracts was investigated by the 1,1-diphenyl-2-picryl-hydrazil (DPPH•) method. Fatty acid composition of oat extract was determined via GC-FID analysis. Total tocopherol content was determined spectrophotometrically. The total antioxidant capacity of the oat grain extract was calculated as 1.5 ± 0.03 mmol TR 100g-1-oat grains extract by CUPRAC method. The DPPH• radical scavenging activity of the oat extract was calculated as 2.02 ± 0.01 mmol TR 100 g-1 – oat extract. Oat extracts exhibited potent antioxidant and radical scavenging ability. GC-FID results revealed that oat extract was rich in unsaturated fatty acids (Oleic acid, 43%; Linoleic acid, 34%). Total tocopherol content of oat oil extracts was 1376.3 ± 16 mg kg-1-oat extract.

Keywords

References

  1. Ak T, Gülçin İ 2008. Antioxidant and Radical Scavenging Properties of Curcumin. Chemico-Biological Interactions, 174(1): 27-37.
  2. Apak R, Güçlü K, Özyürek M, Esin Karademir S, Erçağ E 2006. The Cupric Ion Reducing Antioxidant Capacity and Polyphenolic Content of Some Herbal Teas. International Journal of Food Sciences and Nutrition, 57(5-6): 292-304.
  3. Apak R, Güçlü K, Özyürek M, Karademir SE 2004. Novel Total Antioxidant Capacity Index for Dietary Polyphenols and Vitamins C and E, Using Their Cupric Ion Reducing Capability in The Presence of Neocuproine: CUPRAC Method, Journal of Agricultural and Food Chemistry, 52:7970-7981
  4. Beckman JS, Beckman TW, Chen J, Marshall PA, Freeman BA 1990. Apparent Hydroxyl Radical Production by Peroxynitrite: Implications for Endothelial Injury from Nitric Oxide and Superoxide. Proceedings of The National Academy of Sciences, 87(4): 1620-1624.
  5. Bener M, Şen FB, Önem AN, Bekdeşer B, Çelik SE, Lalikoglu M, Apak R 2022. Microwave-Assisted Extraction of Antioxidant Compounds from by-Products of Turkish Hazelnut (Corylus avellana L.) Using Natural Deep Eutectic Solvents: Modeling, Optimization and Phenolic Characterization. Food Chemistry, 385:132633.
  6. Biel W, Jacyno E, Kawęcka M 2014. Chemical Composition of Hulled, Dehulled and Naked Oat Grains. South African Journal of Animal Science, 44(2): 189-197.
  7. Boukhenouna S, Wilson MA, Bahmed K, Kosmider B 2018. Reactive Oxygen Species in Chronic Obstructive Pulmonary Disease. Oxidative Medicine and Cellular Longevity, 2018.
  8. Bryngelsson S, Mannerstedt‐Fogelfors B, Kamal‐Eldin A, Andersson R, Dimberg LH 2002. Lipids and Antioxidants in Groats and Hulls of Swedish Oats (Avena sativa L). Journal of the Science of Food and Agriculture, 82(6): 606-614.

Details

Primary Language

Turkish

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Publication Date

December 30, 2022

Submission Date

May 1, 2022

Acceptance Date

June 22, 2022

Published in Issue

Year 1970 Volume: 25 Number: Ek Sayı 2

APA
Erdoğan, Ü., & K. Erdoğan, G. (2022). Yulaf (Avena sativa L.) Tanelerinin Ultrasonik Destekli Etanolik Ekstraksiyonunun Toplam Antioksidan Kapasitesi, Radikal Süpürücü Aktivitesi ve Yağ Asidi Kompozisyonunun Belirlenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(Ek Sayı 2), 326-334. https://doi.org/10.18016/ksutarimdoga.vi.1111915

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