Investigation of the Antibiotic Profiles and Phlogenetic Relationships of the Lactobacillus Species Isolated From Goat’s and Cow’s Milk
Abstract
Lactobacillus is naturally abundant in raw milk. Lactobacilli may develop antibiotic resistance as a result of unconscious antibiotic usage in animals. The aim of this study was to identify Lactobacillus species from raw goat’s and cow’s milk, investigate phylogenetic relationships, and examine the antibiotic profiles of these strains. In this study, the milk of 10 cows and 5 goats was obtained from some farms in Adana. The Crystal method was used to phenotypically identify different colonies assumed to be Lactobacillus that were cultured in milk samples. The disc diffusion test was used to determine their resistance to antibiotics. By using the PFGE method, the phylogenetic relationships of Lactobacillus strains were examined. A total of 18 Lactobacillus strains were isolated from 10 different cows’ milk and 10 Lactobacillus strains were identified from 5 distinct goats’ milk. When the antibiotic susceptibility profiles of the Lactobacillus strains isolated from cow’s milk were examined, it was found that all strains were sensitive to vancomycin and chloramphenicol, and 38.9% of them were resistant to some antibiotics. All the Lactobacillus strains isolated from goat’s milk were shown to be susceptible to ampicillin, vancomycin, chloramphenicol, and 40% of all strains were found to be resistant to some antibiotics. The PFGE analysis showed that 28 Lactobacillus strains were separated into 21 pulsetypes, and the strains in the A-B-C-D-E-F-G pulsetypes were found to be 100% similar. Consequently, the sensitivity of Lactobacillus species to antibiotics requires more investigation.
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References
- Barzegar, H., Behbahani, BA., & Falah, F. (2021). Safety, probiotic properties, antimicrobial activity, and technological performance of Lactobacillus strains isolated from Iranian raw milk cheeses. Food Science & Nutrition 9(8), 4094-4107.
- Brennan, NM., Ward, AC., Beresford, PT., Fox, PF., Goodfellow, M., & Cogan, TM. (2002). Biodiversity of the bacterial flora on the surface of a smear cheese. Applied and Environmental Microbiology 68(2), 820-830.
- Bousmaha-Marroki, L., & Marroki, A. (2015). Antibiotic susceptibility and heterogeneity in technological traits of Lactobacilli isolated from Algerian goat’s milk. Journal of Food Science and Technology 52(8), 4708-4723.
- Danielsen, M., & Wind, A. (2003). Susceptibility of Lactobacillus spp. to antimicrobial agents. International Journal of Food Microbiology 82, 1-11.
- Erginkaya, Z., Turhan, EU., & Tatlı, D. (2018). Determination of antibiotic resistance of lactic acid bacteria isolated from traditional Turkish fermented dairy products. Iranian Journal of Veterinary Research 19(1), 53-56.
- Gad, GFM., Abdel-Hamid, AM., & Farag, ZSHF. (2014). Antibiotic resistance in lactic acid bacteria isolated from some pharmaceutical and dairy products. Brazilian Journal of Microbiology 45(1), 25-33.
- Georgalaki M., Zoumpopoulou, G., Anastasiou, R., Kazou, M., & Tsakalidou, E. (2021). Lactobacillus kefiranofaciens: From isolation and taxonomy to probiotic properties and applications. Microorganisms 9(10), 2158.
- Hleba, L., Kačániová, M., Pavelková, A., & Čuboň, J. (2012). Antibiotic resistance of Lactobacilli strains isolated from milk and milk products from Middle Slovakia. Journal of Microbiology, Biotechnology and Food Sciences 2, 253-262.
- Jaimee, G., & Halami, PM. (2016). High level aminoglycoside resistance in Enterococcus, Pediococcus and Lactobacillus species from farm animals and commercial meat products. Annals of Microbiology 66, 101-110.
- Kızılyıldırım, S., & Köksal, F. (2021). Asemptomatik kadınlarda vajinal ve rektal laktobasillerin tespiti ve ilişkilerinin belirlenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi 16(2), 219-225.
- Marroki, A., & Bousmaha-Marroki, L. (2014). Lactobacilli isolated from Algerian goat’s milk as adjunct culture in dairy products. Brazilian Archives of Biology and Technology 57(3), 410-420.
- Marroki, A., Zúñiga, M., Kihal, M., & Pérez- Martínez, G. (2011). Characterization of Lactobacillus from Algerian goat’s milk based on phenotypic, 16SrDNA sequencing and their technological properties. Brazilian Journal of Microbiology 42, 158-171.
- Reuben, RC., Roy, PC., Sarkar, SL., Alam, ASMRU., & Jahid, IK. (2020). Characterization and evaluation of lactic acid bacteria from indigenous raw milk for potential probiotic properties. Journal of Dairy Science 103(2), 1223-1237.
- Wang, K., Zhang, H., Feng, J., Ma, L., Fuente-Núñez, C., Wang, S., & Lu, X. (2019). Antibiotic resistance of lactic acid bacteria isolated from dairy products in Tianjin, China. Journal of Agriculture and Food Research 1, 100006.
- Xu, F.L, Guo, YC., Wang, HX., Fu, P., Zeng, HW., Li, ZG., Pei, XY., & Liu, XM. (2012). PFGE genotyping and antibiotic resistance of Lactobacillus distributed strains in the fermented dairy products. Annals of Microbiology 62, 255-262.
- Villavicencio, J., Villegas, LM., Arango, MC., Arias, S., & Triana, F. (2018). Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial. Journal of Applied Oral Science 26, 20170318.
- Zawistowska-Rojek, A., & Tyski, S. (2018). Are probiotic really safe for humans? Polish Journal of Microbiology 67(3), 251-258.
- Zhang, Z., Lv, J., Pan, L., & Zhang, Y. (2018). Roles and applications of probiotic Lactobacillus strains. Applied Microbiology and Biotechnology 102, 8135-8143.
Keçi ve İnek Sütünden İzole Edilen Lactobacillus Türlerinin Antibiyotik Profillerinin ve Filogenetik İlişkilerinin AraştırılmasıKeçi ve İnek Sütünden İzole Edilen Lactobacillus Türlerinin Antibiyotik Profillerinin ve Filogenetik İlişkilerinin Araştırılması
Abstract
Lactobacillus, çiğ sütte bol miktarda ve doğal olarak bulunur. Hayvanlarda bilinçsiz antibiyotik kullanımı sonucu, laktobasiller antibiyotik direnci geliştirebilir. Bu çalışmanın amacı çiğ keçi ve inek sütünden elde edilen Lactobacillus türlerini belirlemek, filogenetik ilişkileri araştırmak ve bu suşların antibiyotik profillerini incelemektir. Çalışmada Adana’daki bazı çiftliklerden 10 inek ve 5 keçi sütü elde edilmiştir. Kristal yöntemi, süt örneklerinde kültürlenen Lactobacillus olduğu varsayılan farklı kolonileri fenotipik olarak tanımlamak için kullanıldı. Antibiyotiklere dirençlerini belirlemek için disk difüzyon testi kullanıldı. PFGE yöntemi kullanılarak Lactobacillus suşlarının filogenetik ilişkileri incelendi. 10 farklı inek sütünden 18 Lactobacillus suşu izole edildi ve 5 farklı keçi sütünden 10 Lactobacillus suşu tanımlandı. İnek sütünden izole edilen Lactobacillus suşlarının antibiyotik duyarlılık profilleri incelendiğinde, bakterilerin tamamının vankomisin ve kloramfenikole duyarlı olduğu, %38,9'unun bazı antibiyotiklere dirençli olduğu tespit edildi. Keçi sütünden izole edilen tüm Lactobacillus suşlarının ampisilin, vankomisin, kloramfenikol'e duyarlı olduğu gösterilmiş ve bunların %40'ının bazı antibiyotiklere dirençli olduğu saptanmıştır. PFGE analizi 28 Lactobacillus suşunun 21 pulsetipine ayrıldığını ve A-B-C-D-E-F-G pulsetiplerindeki suşların %100 benzer olduğunu göstermiştir. Sonuç olarak, Lactobacillus türlerinin antibiyotik duyarlılıkları konusunda daha kapsamlı araştırmaların yapılması gerekmektedir.
Keywords
Project Number
-
References
- Barzegar, H., Behbahani, BA., & Falah, F. (2021). Safety, probiotic properties, antimicrobial activity, and technological performance of Lactobacillus strains isolated from Iranian raw milk cheeses. Food Science & Nutrition 9(8), 4094-4107.
- Brennan, NM., Ward, AC., Beresford, PT., Fox, PF., Goodfellow, M., & Cogan, TM. (2002). Biodiversity of the bacterial flora on the surface of a smear cheese. Applied and Environmental Microbiology 68(2), 820-830.
- Bousmaha-Marroki, L., & Marroki, A. (2015). Antibiotic susceptibility and heterogeneity in technological traits of Lactobacilli isolated from Algerian goat’s milk. Journal of Food Science and Technology 52(8), 4708-4723.
- Danielsen, M., & Wind, A. (2003). Susceptibility of Lactobacillus spp. to antimicrobial agents. International Journal of Food Microbiology 82, 1-11.
- Erginkaya, Z., Turhan, EU., & Tatlı, D. (2018). Determination of antibiotic resistance of lactic acid bacteria isolated from traditional Turkish fermented dairy products. Iranian Journal of Veterinary Research 19(1), 53-56.
- Gad, GFM., Abdel-Hamid, AM., & Farag, ZSHF. (2014). Antibiotic resistance in lactic acid bacteria isolated from some pharmaceutical and dairy products. Brazilian Journal of Microbiology 45(1), 25-33.
- Georgalaki M., Zoumpopoulou, G., Anastasiou, R., Kazou, M., & Tsakalidou, E. (2021). Lactobacillus kefiranofaciens: From isolation and taxonomy to probiotic properties and applications. Microorganisms 9(10), 2158.
- Hleba, L., Kačániová, M., Pavelková, A., & Čuboň, J. (2012). Antibiotic resistance of Lactobacilli strains isolated from milk and milk products from Middle Slovakia. Journal of Microbiology, Biotechnology and Food Sciences 2, 253-262.
- Jaimee, G., & Halami, PM. (2016). High level aminoglycoside resistance in Enterococcus, Pediococcus and Lactobacillus species from farm animals and commercial meat products. Annals of Microbiology 66, 101-110.
- Kızılyıldırım, S., & Köksal, F. (2021). Asemptomatik kadınlarda vajinal ve rektal laktobasillerin tespiti ve ilişkilerinin belirlenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi 16(2), 219-225.
- Marroki, A., & Bousmaha-Marroki, L. (2014). Lactobacilli isolated from Algerian goat’s milk as adjunct culture in dairy products. Brazilian Archives of Biology and Technology 57(3), 410-420.
- Marroki, A., Zúñiga, M., Kihal, M., & Pérez- Martínez, G. (2011). Characterization of Lactobacillus from Algerian goat’s milk based on phenotypic, 16SrDNA sequencing and their technological properties. Brazilian Journal of Microbiology 42, 158-171.
- Reuben, RC., Roy, PC., Sarkar, SL., Alam, ASMRU., & Jahid, IK. (2020). Characterization and evaluation of lactic acid bacteria from indigenous raw milk for potential probiotic properties. Journal of Dairy Science 103(2), 1223-1237.
- Wang, K., Zhang, H., Feng, J., Ma, L., Fuente-Núñez, C., Wang, S., & Lu, X. (2019). Antibiotic resistance of lactic acid bacteria isolated from dairy products in Tianjin, China. Journal of Agriculture and Food Research 1, 100006.
- Xu, F.L, Guo, YC., Wang, HX., Fu, P., Zeng, HW., Li, ZG., Pei, XY., & Liu, XM. (2012). PFGE genotyping and antibiotic resistance of Lactobacillus distributed strains in the fermented dairy products. Annals of Microbiology 62, 255-262.
- Villavicencio, J., Villegas, LM., Arango, MC., Arias, S., & Triana, F. (2018). Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial. Journal of Applied Oral Science 26, 20170318.
- Zawistowska-Rojek, A., & Tyski, S. (2018). Are probiotic really safe for humans? Polish Journal of Microbiology 67(3), 251-258.
- Zhang, Z., Lv, J., Pan, L., & Zhang, Y. (2018). Roles and applications of probiotic Lactobacillus strains. Applied Microbiology and Biotechnology 102, 8135-8143.
Details
| Primary Language | English |
|---|---|
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Project Number | - |
| Early Pub Date | May 27, 2023 |
| Publication Date | October 31, 2023 |
| Submission Date | September 21, 2022 |
| Acceptance Date | February 27, 2023 |
| Published in Issue | Year 2023Volume: 26 Issue: 5 |
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Geleneksel Türk Yoğurtlarından İzole Edilen Lactobacillus delbrueckii subsp. bulgaricus ve Streptococcus thermophilus Bakterilerinde İntra-/Ekstrasellüler Folat Üretimi
Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi
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