Evaluation of the in vitro enzyme inhibition and antioxidant activity of Clinopodium betulifolium (Boiss. & Balansa) Kuntze

Nuraniye Eruygur; Tuğsen Büyükyıldırım; Damla Kırcı; Fatma Ayaz; Yavuz Bağcı; Süleyman Doğu

doi:10.18016/ksutarimdoga.vi.1403319

TR EN

Clinopodium betulifolium (Boiss. & Balansa) Kuntze'nin in vitro enzim inhibisyonu ve antioksidan aktivitesinin değerlendirilmesi

Öz

Clinopodium betulifolium (Boiss. & Balansa) Kuntze, Lamiaceae familyasına ait çok yıllık bir bitkidir. C. betulifolium üzerine uçucu yağı dışında pek fazla çalışma bulunmamaktadır. Bu çalışmada C. betulifolium türünün Alzheimer ve kozmetikle ilişkili enzim inhibitör aktivitesi ve antioksidan aktivitesi değerlendirildi. Bu çalışmada C. betulifolium'un toprak üstü kısımlarından %70 metanol ve su kullanılarak maserasyon tekniği ile ekstraksiyon işlemi yapıldı. C. betulifolium ekstrelerinin antioksidan [DPPH radikal süpürücü aktivite, ABTS katyonik renk giderici ve demir şelatlama aktivitesi] ve enzim inhibisyonu (asetil/bütirilkolinesteraz ve tirozinaz) aktiviteleri, 2 mg mL-1 stok konsantrasyonunda Elisa mikroplaka okuyucusu kullanılarak değerlendirildi. C. betulifolium su ekstresi ABTS yönteminde yüksek antioksidan aktivite verirken (IC50: 34.24 ± 5.01 µg mL-1), DPPH yönteminde %70 metanol ekstresi (IC50: 100.75 ± 2.62 µg mL-1) sulu ekstresinden (IC50: 131,83 ± 4,70 µg mL-1) daha yüksek aktivitede bulunmuştur. C. betulifolium su ve %70 metanol ekstreleri orta derecede anti-tirozinaz aktivitesine sahiptir. Hem %70 metanol hem de su ekstreleri sırasıyla 73,94 ± 2,78 µg mL-1 ve 81,71 ± 9,38 µg mL-1 IC50 değerleriyle asetilkolinesteraza karşı benzer ve yüksek aktivite gösterdi. C. betulifolium %70 metanol ekstresi (IC50: 64,08 ± 1,04 µg mL-1), bütirilkolinesteraza karşı su ekstresinden (IC50: 146,6 ± 8,27 µg mL-1) daha yüksek inhibitör aktivite gösterdi. Bu sonuçlar Alzheimer ve oksidatif strese bağlı hastalıkların tedavisinde kullanılacak farmasötik formülasyonların veya gıda takviyelerinin geliştirilmesinde olumlu sonuçlar verecek çalışmalar için temel bilgiler sağlamaktadır.

Anahtar Kelimeler

Evaluation of the in vitro enzyme inhibition and antioxidant activity of Clinopodium betulifolium (Boiss. & Balansa) Kuntze

Abstract

Clinopodium betulifolium (Boiss. & Balansa) Kuntze is a perennial herb belonging to the Lamiaceae family. There are few studies on C. betulifolium, except for its essential oil. In this study, Alzheimer's and cosmetic-related enzyme inhibitory activity and antioxidant activity of C. betulifolium species were evaluated. This study extracted C. betulifolium aerial parts by maceration using 70% methanol and water. Antioxidant [DPPH scavenging assay, ABTS cation decolorization, and iron chelating activity] and enzyme inhibition (acetyl-, and butyrylcholine esterase, and tyrosinase) activities of C. betulifolium extracts were evaluated using Elisa microplate reader at 2 mg mL-1 stock concentration. C. betulifolium aqueous extract gave high antioxidant activity (IC50: 34.24 ± 5.01 µg mL-1) in the ABTS method, while its 70% methanol extract (IC50: 100.75 ± 2.62 µg mL-1) was higher than the aqueous extract (IC50: 131.83 ± 4.70 µg mL-1) in the DPPH method. C. betulifolium aqueous and 70% methanol extract have moderate anti-tyrosinase activity. Both 70% methanol and aqueous extracts showed similar and high activity against acetylcholinesterase with the IC50 values of 73.94 ± 2.78 µg mL-1 and 81.71 ± 9.38 µg mL-1, respectively. C. betulifolium 70% methanol extract (IC50: 64.08 ± 1.04 µg mL-1) showed higher inhibitory activity than the aqueous extract (IC50: 146.6 ± 8.27 µg mL-1) against butyrylcholinesterase. These results provide basic information for studies that will yield positive results in the development of pharmaceutical formulations or food supplements to be used to treat Alzheimer's and oxidative stress-related diseases.

Keywords

Supporting Institution

Selçuk Üniversitesi Bilimsel Araştırmalar Koordinatörlüğü

Thanks

Bu çalışmanın gercekleşmesinde maddi imkan sağlayan Selçuk Üniversitesi. Bilimsel Araştırma Projeleri (BAP) birimine katkılarından dolayı teşekkür ederiz.

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Nuraniye Eruygur ^*
0000-0002-4674-7009
Türkiye

Tuğsen Büyükyıldırım
0000-0003-0101-9742
Türkiye

Damla Kırcı
0000-0002-3479-3999
Türkiye

Fatma Ayaz
0000-0003-3994-6576
Türkiye

Yavuz Bağcı
0000-0002-2343-3672
Türkiye

Süleyman Doğu
0000-0002-5352-9288
Türkiye

Early Pub Date

August 15, 2024

Publication Date

November 7, 2024

Submission Date

December 12, 2023

Acceptance Date

April 22, 2024

Published in Issue

Year 2024 Volume: 27 Number: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1403319

IZ

https://izlik.org/JA42FF35GA

Cite

RIS / Bibtex

APA

Eruygur, N., Büyükyıldırım, T., Kırcı, D., Ayaz, F., Bağcı, Y., & Doğu, S. (2024). Evaluation of the in vitro enzyme inhibition and antioxidant activity of Clinopodium betulifolium (Boiss. & Balansa) Kuntze. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(6), 1282-1288. https://doi.org/10.18016/ksutarimdoga.vi.1403319

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