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Üzüm Yapraklarındaki Klorofil ve Renk Içeriğinin Iki Asma Büyüme Evresinde (Çiçeklenme ve Tane Tutumu) Belirlenmesi

Year 2025, Volume: 28 Issue: 3, 757 - 763

Turhan Yılmaz , Abdurrahim Bozkurt , Adem Yağcı

https://doi.org/10.18016/ksutarimdoga.vi.1609921

Abstract

Klorofil konsantrasyonu yaprağın fotosentez yapma yeteneğinin bir ölçüsüdür. Bu bitkilerin fizyolojik durumunu ilerletmek için önemlidir. SPAD metreler tarafından sıklıkla yaprak klorofil konsantrasyonunun hızlı bir şekilde tahmin edilebilmektedir. SPAD metreler bitki türlerinin büyüme koşullarındaki değişiklikler dikkate alınarak yaprak klorofil içeriğini doğru bir şekilde ölçmek için kullanılabilirler. Bu çalışmanın amacı, salamura için üzüm yapraklarındaki SPAD değerlerinden klorofil (a*, b*) ve L* (Parlaklık) seviyelerini iki farklı asma büyüme aşaması olan çiçeklenme ve tane tutma sırasında hesaplamaktır. Bu çalışmada, Narince x Isabella kombinasyonundan 189, Narince x Kishmish Vatkana kombinasyonundan 39, Narince x Regent kombinasyonundan ise 218 bitki kullanılmıştır. Ayrıca, yerel gen kaynaklarımızdan Narince, Künefi, Erciş, Dökülgen, Fenerit, Hatun Parmağı, Horoz Karası, Muhammedi, Karaerik ve Vakkas üzüm çeşitleri ile küllemeye duyarlı olarak bilinen Italia ve Kyoho çeşitleri dahil edilmiştir. Bu çalışmada, klorofil içeriği (L*, a* ve b* konsantrasyonları için) çiçeklenmeden tane tutumuna istatistiksel olarak azalmıştır. Genotipler ve dönemler hem ana faktör hemde etkileşim faktörleri için farklılıklar göstermektedir. Burada bildirilen çalışma hibrit ve kültür asması çeşitleri arasında, klorofil a*/b* oranının farklılaştırıldığını göstermektedir. Çalışmanın sonuçlarına göre, L*, a* ve b* konsantrasyonları çiçeklenmeden tane tutumu evresine doğru azalmıştır. Bu çalışma, bitki türlerindeki çeşitli gelişim evrelerinin yaprak klorofil içeriği ile SPAD okumaları arasındaki bağlantıyı etkileyebileceğini göstermektedir.

Keywords

SPAD Klorofil Renk SPAD Asma Fotosentez

References

  • Anand, M. H., & Byju, G. (2008). Chlorophyll meter and leaf colour chart to estimate chlorophyll content, leaf colour, and yield of cassava. Photosynthetica, 46(4), 511-516.
  • Bullock, D. G., & Anderson, D. S. (1998). Evaluation of the Minolta SPAD‐502 chlorophyll meter for nitrogen management in corn. Journal of Plant Nutrition, 21(4), 741-755.
  • Campbell, R. J., Mobley, K. N., Marini, R. P., & Pfeiffer, D. G. (1990). Growing conditions alter the relationship between SPAD-501 values and apple leaf chlorophyll. HortScience, 25(3), 330-331.
  • Chen, K., & Roca, M. (2018). In vitro bioavailability of chlorophyll pigments from edible seaweeds. Journal of Functional Foods, 41, 25-33.
  • Fanizza, G., Gatta, C. D., & Bagnulo, C. (1991). A non‐destructive determination of leaf chlorophyll in Vitis vinifera. Annals of applied biology, 119(1), 203-205.
  • Jiang, C., Johkan, M., Hohjo, M., Tsukagoshi, S., & Maruo, T. (2017). A correlation analysis on chlorophyll content and SPAD value in tomato leaves. HortResearch, 71, 37-42.
  • Kılıç, D. (2007). Effects of different pruning levels and nitrogen doses on yield and quality of grape leaves in Narince grape variety (Thesis no: 199923) [Master’s thesis, Tokat Gazi Osmanpaşa University, Institute of Science, Department of Horticulture]. Council of Higher Education Thesis Center. https://tez.yok.gov.tr/ UlusalTezMerkezi/
  • León, A. P., Viña, S. Z., Frezza, D., Chaves, A., & Chiesa, A. (2007). Estimation of chlorophyll contents by correlations between SPAD‐502 meter and chroma meter in butterhead lettuce. Communications in soil science and plant analysis, 38(19-20), 2877-2885.
  • Ling, Q., Weihua Huang, W. & Jarvis, P. (2011). Use of a SPAD-502 meter to measure leaf chlorophyll concentration in Arabidopsis thaliana. Photosynthesis Research, 107, 209-214.
  • Madeira, A., Ferreira, A., De Varennes, A., and Vieira, M. (2003) SPAD meter versus tristimulus colorimeter to estimate chlorophyll content and leaf color in sweet pepper.Communications in soil science and plant analysis, 34(17-18), 2461-2470.
  • Markwell, J., Osterman, J. C., & Mitchell, J. L. (1995). Calibration of the Minolta SPAD-502 leaf chlorophyll meter. Photosynthesis research, 46, 467-472.
  • Monje, O. A., & Bugbee, B. (1992). Inherent limitations of nondestructive chlorophyll meters: a comparison of two types of meters. HortScience, 27(1), 69-71.
  • Mürtezaoğlu, Ç, 2015. Salamura Asma Yaprağının Lifçe Zengin Bileşeninin Fizikokimyasal Özellikleri ve In Vitro Hipoglisemik Etkisi. (Tez no: 172141) [Yüksek Lisans Tezi, Istanbul Teknik Üniv. Fen Bil. Ens. Gıda Müh. Anabilim Dalı] Yükseköğretim Kurulu Ulusal Tez Merkezi.
  • Netto, A. T., Campostrini, E., de Oliveira, J. G., & Bressan-Smith, R. E. (2005). Photosynthetic pigments, nitrogen, chlorophyll a fluorescence and SPAD-502 readings in coffee leaves. Scientia horticulturae, 104(2), 199-209.
  • Rajalakshmi, K., & Banu, N. (2015). Extraction and estimation of chlorophyll from medicinal plants. International Journal of Science and Research, 4(11), 209-212.
  • R Core Team. R: A language and environment for statistical computing; R Foundation for Statistical Computing: Vienna, Austria, 2013. http:// www.R‐project.org/ Accessed 8 August 2024.
  • Richardson, A. D., Duigan, S. P., & Berlyn, G. P. (2002). An evaluation of noninvasive methods to estimate foliar chlorophyll content. New phytologist, 153(1), 185-194.
  • Ruiz-Espinoza FH, Murillo-Amador B, García-Hernández JL, Fenech-Larios L, Rueda-Puente EO, Troyo-Diéguez E, Beltrán-Morales A (2010). Field evaluation of the relationship between chlorophyll content in basil leaves and a portable chlorophyll meter (SPAD-502) readings. Journal of Plant Nutrition 33(3), 423-438.
  • Samdur, M. Y., A. L. Singh, R. K. Mathur, P. Manivel, B. M. Chikani, H. K. Gor, andM. A. Khan. 2000. Field evaluation of chlorophyll meter for screening groundnut (Arachis hypogaea L.) genotypes tolerant to iron-deficiency chlorosis. Current Science 79, 211–214
  • Shibaeva, T. G., Mamaev, A. V., & Sherudilo, E. G. (2020). Evaluation of a SPAD-502 plus chlorophyll meter to estimate chlorophyll content in leaves with interveinal chlorosis. Russian Journal of Plant Physiology, 67, 690-696.
  • Uddling, J., J. Gelang-Alfredsson, K. Piikki, and H. Pleijel. 2007. Evaluating the relationship between leaf chlorophyll concentration and SPAD-502 chlorophyll meter readings. Photosynthesis Research 91, 37–46.
  • Underhill, S. J., & Critchley, C. (1995). Cellular localisation of polyphenol oxidase and peroxidase activity in Litchi chinensis Sonn. pericarp. Functional Plant Biology, 22(4), 627-632.
  • Yamamoto, A., Nakamura, T., Adu-Gyamfi, J. J., & Saigusa, M. (2002). Relationship between chlorophyll content in leaves of sorghum and pigeonpea determined by extraction method and by chlorophyll meter (SPAD-502). Journal of Plant Nutrition, 25(10), 2295-2301.
  • Yang, H., Yang, J., Lv, Y., & He, J. (2014). SPAD values and nitrogen nutrition index for the evaluation of rice nitrogen status. Plant Production Science, 17(1), 81-92.
  • Zhang, R., Yang, P., Liu, S., Wang, C., & Liu, J. (2022). Evaluation of the methods for estimating leaf chlorophyll content with SPAD chlorophyll meters. Remote Sensing, 14(20), 5144.
  • Zhang, Y., Li, Y., Wang, R., Xu, L., Li, M., Liu, Z., ... & He, N. (2020). Spatial variation of leaf chlorophyll in northern hemisphere grasslands. Frontiers in Plant Science, 11, 1244.
  • Zielewicz, W., Wróbel, B., & Niedbała, G. (2020). Quantification of chlorophyll and carotene pigments content in mountain melick (Melica nutans L.) in relation to edaphic variables. Forests, 11(11), 1197.
  • Zotarelli, L., Cardoso, E., Piccinin, J., Urquiaga, S., Boddey, M., Torres, E., and Alves, B. (2003) Calibration of a Minolta SPAD-502 chlorophyll meter for evaluation of the nitrogen nutrition of maize. Pesquisa Agropecua´ria Brasileira, 38, 1117–1122.

Identification of chlorophyll and color content in grape leaves during two growth stages (flowering and setting)

Year 2025, Volume: 28 Issue: 3, 757 - 763

Turhan Yılmaz , Abdurrahim Bozkurt , Adem Yağcı

https://doi.org/10.18016/ksutarimdoga.vi.1609921

Abstract

Chlorophyll concentration is a measure of the leaf's ability to photosynthesize. It is essential for advancing the physiological state of plants. A rapid estimate of in situ leaf chlorophyll concentration is frequently provided by SPAD meters. When utilizing SPAD meters to accurately quantify leaf chlorophyll content, it is important to consider the variations in growth conditions of vegetation species. This study aimed to calculate the chlorophyll (a*, b*) and L* (Lightness) levels from SPAD values in grape leaves for pickling during two vine different growth stages: blooming and set. In this study, 189 plants from Narince x Isabella combination, 39 plants from Narince x Kishmish Vatkana combination, and 218 plants from Narince x Regent combination were used. In addition, Narince, Künefi, Erciş, Dökülgen, Fenerit, Hatun Parmağı, Horoz Karası, Muhammedi, Karaerik, and Vakkas grape varieties from our local germplasm and Italia and Kyoho varieties known to be susceptible to powdery mildew were included. In this study, chlorophyll content has decreased statistically from flowering to setting for L*, a*, and b* concentration. Analyzing two factors (genotypes and periods) have statistical differences for both main and interactions. The study reported here indicates that a statistical separation ratio of chlorophyll a*/b* was discovered. According to the results of the study, concentrations of L*, a*, and b* decreased from flowering to berry set phases. This study points out that various developmental stages in plant species might affect the link between leaf chlorophyll content and SPAD readings.

Keywords

Chlorophyll SPAD Color Grapevine Photosynthesis

References

  • Anand, M. H., & Byju, G. (2008). Chlorophyll meter and leaf colour chart to estimate chlorophyll content, leaf colour, and yield of cassava. Photosynthetica, 46(4), 511-516.
  • Bullock, D. G., & Anderson, D. S. (1998). Evaluation of the Minolta SPAD‐502 chlorophyll meter for nitrogen management in corn. Journal of Plant Nutrition, 21(4), 741-755.
  • Campbell, R. J., Mobley, K. N., Marini, R. P., & Pfeiffer, D. G. (1990). Growing conditions alter the relationship between SPAD-501 values and apple leaf chlorophyll. HortScience, 25(3), 330-331.
  • Chen, K., & Roca, M. (2018). In vitro bioavailability of chlorophyll pigments from edible seaweeds. Journal of Functional Foods, 41, 25-33.
  • Fanizza, G., Gatta, C. D., & Bagnulo, C. (1991). A non‐destructive determination of leaf chlorophyll in Vitis vinifera. Annals of applied biology, 119(1), 203-205.
  • Jiang, C., Johkan, M., Hohjo, M., Tsukagoshi, S., & Maruo, T. (2017). A correlation analysis on chlorophyll content and SPAD value in tomato leaves. HortResearch, 71, 37-42.
  • Kılıç, D. (2007). Effects of different pruning levels and nitrogen doses on yield and quality of grape leaves in Narince grape variety (Thesis no: 199923) [Master’s thesis, Tokat Gazi Osmanpaşa University, Institute of Science, Department of Horticulture]. Council of Higher Education Thesis Center. https://tez.yok.gov.tr/ UlusalTezMerkezi/
  • León, A. P., Viña, S. Z., Frezza, D., Chaves, A., & Chiesa, A. (2007). Estimation of chlorophyll contents by correlations between SPAD‐502 meter and chroma meter in butterhead lettuce. Communications in soil science and plant analysis, 38(19-20), 2877-2885.
  • Ling, Q., Weihua Huang, W. & Jarvis, P. (2011). Use of a SPAD-502 meter to measure leaf chlorophyll concentration in Arabidopsis thaliana. Photosynthesis Research, 107, 209-214.
  • Madeira, A., Ferreira, A., De Varennes, A., and Vieira, M. (2003) SPAD meter versus tristimulus colorimeter to estimate chlorophyll content and leaf color in sweet pepper.Communications in soil science and plant analysis, 34(17-18), 2461-2470.
  • Markwell, J., Osterman, J. C., & Mitchell, J. L. (1995). Calibration of the Minolta SPAD-502 leaf chlorophyll meter. Photosynthesis research, 46, 467-472.
  • Monje, O. A., & Bugbee, B. (1992). Inherent limitations of nondestructive chlorophyll meters: a comparison of two types of meters. HortScience, 27(1), 69-71.
  • Mürtezaoğlu, Ç, 2015. Salamura Asma Yaprağının Lifçe Zengin Bileşeninin Fizikokimyasal Özellikleri ve In Vitro Hipoglisemik Etkisi. (Tez no: 172141) [Yüksek Lisans Tezi, Istanbul Teknik Üniv. Fen Bil. Ens. Gıda Müh. Anabilim Dalı] Yükseköğretim Kurulu Ulusal Tez Merkezi.
  • Netto, A. T., Campostrini, E., de Oliveira, J. G., & Bressan-Smith, R. E. (2005). Photosynthetic pigments, nitrogen, chlorophyll a fluorescence and SPAD-502 readings in coffee leaves. Scientia horticulturae, 104(2), 199-209.
  • Rajalakshmi, K., & Banu, N. (2015). Extraction and estimation of chlorophyll from medicinal plants. International Journal of Science and Research, 4(11), 209-212.
  • R Core Team. R: A language and environment for statistical computing; R Foundation for Statistical Computing: Vienna, Austria, 2013. http:// www.R‐project.org/ Accessed 8 August 2024.
  • Richardson, A. D., Duigan, S. P., & Berlyn, G. P. (2002). An evaluation of noninvasive methods to estimate foliar chlorophyll content. New phytologist, 153(1), 185-194.
  • Ruiz-Espinoza FH, Murillo-Amador B, García-Hernández JL, Fenech-Larios L, Rueda-Puente EO, Troyo-Diéguez E, Beltrán-Morales A (2010). Field evaluation of the relationship between chlorophyll content in basil leaves and a portable chlorophyll meter (SPAD-502) readings. Journal of Plant Nutrition 33(3), 423-438.
  • Samdur, M. Y., A. L. Singh, R. K. Mathur, P. Manivel, B. M. Chikani, H. K. Gor, andM. A. Khan. 2000. Field evaluation of chlorophyll meter for screening groundnut (Arachis hypogaea L.) genotypes tolerant to iron-deficiency chlorosis. Current Science 79, 211–214
  • Shibaeva, T. G., Mamaev, A. V., & Sherudilo, E. G. (2020). Evaluation of a SPAD-502 plus chlorophyll meter to estimate chlorophyll content in leaves with interveinal chlorosis. Russian Journal of Plant Physiology, 67, 690-696.
  • Uddling, J., J. Gelang-Alfredsson, K. Piikki, and H. Pleijel. 2007. Evaluating the relationship between leaf chlorophyll concentration and SPAD-502 chlorophyll meter readings. Photosynthesis Research 91, 37–46.
  • Underhill, S. J., & Critchley, C. (1995). Cellular localisation of polyphenol oxidase and peroxidase activity in Litchi chinensis Sonn. pericarp. Functional Plant Biology, 22(4), 627-632.
  • Yamamoto, A., Nakamura, T., Adu-Gyamfi, J. J., & Saigusa, M. (2002). Relationship between chlorophyll content in leaves of sorghum and pigeonpea determined by extraction method and by chlorophyll meter (SPAD-502). Journal of Plant Nutrition, 25(10), 2295-2301.
  • Yang, H., Yang, J., Lv, Y., & He, J. (2014). SPAD values and nitrogen nutrition index for the evaluation of rice nitrogen status. Plant Production Science, 17(1), 81-92.
  • Zhang, R., Yang, P., Liu, S., Wang, C., & Liu, J. (2022). Evaluation of the methods for estimating leaf chlorophyll content with SPAD chlorophyll meters. Remote Sensing, 14(20), 5144.
  • Zhang, Y., Li, Y., Wang, R., Xu, L., Li, M., Liu, Z., ... & He, N. (2020). Spatial variation of leaf chlorophyll in northern hemisphere grasslands. Frontiers in Plant Science, 11, 1244.
  • Zielewicz, W., Wróbel, B., & Niedbała, G. (2020). Quantification of chlorophyll and carotene pigments content in mountain melick (Melica nutans L.) in relation to edaphic variables. Forests, 11(11), 1197.
  • Zotarelli, L., Cardoso, E., Piccinin, J., Urquiaga, S., Boddey, M., Torres, E., and Alves, B. (2003) Calibration of a Minolta SPAD-502 chlorophyll meter for evaluation of the nitrogen nutrition of maize. Pesquisa Agropecua´ria Brasileira, 38, 1117–1122.
There are 28 citations in total.

Details

Primary Language English
Subjects Oenology and Viticulture
Journal Section RESEARCH ARTICLE
Authors

Turhan Yılmaz 0000-0002-3756-4497

Abdurrahim Bozkurt 0000-0001-7315-202X

Adem Yağcı 0000-0002-3650-4679

Early Pub Date May 1, 2025
Publication Date
Submission Date December 30, 2024
Acceptance Date April 16, 2025
Published in Issue Year 2025Volume: 28 Issue: 3

Cite

APA Yılmaz, T., Bozkurt, A., & Yağcı, A. (2025). Identification of chlorophyll and color content in grape leaves during two growth stages (flowering and setting). Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(3), 757-763. https://doi.org/10.18016/ksutarimdoga.vi.1609921
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