Research Article
Year 2021,
Volume: 11 Issue: 1, 104 - 117, 15.06.2021
Abstract
The fresh stem and leaves Rheum ribes, species of Polygonaceae family, are consumed as vegetable in Lebanon, Iran and eastern Turkey. Recently, crude drug materials from this plant are used in pharmacological research in Asian countries. In this study, in vitro phytochemical, antioxidant and antibacterial activity of the extracts prepared at 10-100 mg/mL concentrations by using ethyl acetate, hexane, methanol and distilled water from stem parts of Rheum ribes were examined. The extraction efficiency varies depending on the solvents and the highest extraction efficiency was calculated as %25.3 at methanol extract. The highest total phenolic and flavonoid contents were determined 0.80 ± 0.00 mg GAE/g and 0.321 ± 0.00 mg RE/g at 100 mg/mL concentration of methanolic extract. DPPH· radical scavenging activity of methanolic stem extract was %91.10±0.51 (IC50 dose 10.50 mg/mL). The highest Fe(II) ions chelating activity (49.70±3.10%), reduction capacity (0.19±0.00) and superoxide anion scavenging activity (39.20 ± 2.40%) were recorded at methanol extract. Ethyl acetate, hexane and methanol extracts of Rheum ribes not showed antibacterial activity against test bacteria. Antibacterial activity of distilled water extract was in the range of 9.00±1.00 and 14.50±1.50 mm. The highest inhibitory effect was observed against S. aureus with 14.5 ±1.50 mm zone diameter.
Keywords
References
- Andiç, S., Tuncturk, Y., Ocak, E., and Kose, S., (2009). Some chemica characteristic of edible wild rhubarb species (Rheum ribes L.). Research Journal of Agriculture and Biological Sciences, 5(6), 973-977.
- Bauer, A. W., Kirby, W. M., Sherris, J. C., and Turck, M., (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45(4), 493-496.
- Blois, M. S., (1958). Antioxidant determinations by the use of a stable free radical. Nature, 26, 1199-1200.
- Davis, P. H., (1967). Flora of Turkey and The Aegean Islands, Edinburg Universiyt Press
- Dinis, T. C., Madeira, V. M., and Almeida, L. M., (1994). Action of phenolic derivates (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics, 315(1), 161-169.
- Güvenç, İ., Kaya, Y., (1996). Erzurum'da sebze olarak değerlendirilen yöresel bazı bitkiler. Ankara Üniversitesi Ziraat Fakültesi Dergisi, 27(3): 369-374.
- Khalida, K. A., Ehklass, M. T., and Saleh, R. S., (2014). Phenolic profile, antioxidant, and antibacterial effects of ethanol and aqueous extracts of Rheum ribes L. Roots, Der Pharmacia Lettre, 6(5), 201-205.
- Kosikowska, U., Smolarz, H. D., and Malm, A., (2010). Antimicrobial activity and total content of polyphenols of Rheum L. species growing in Poland, Current Europan Journal of Biology, 5(6), 814-820.
- Memişoğulları, R., 2005. Diyabette serbest radikallerin rolü ve antioksidanların etkisi. Düzce Tıp Fakültesi Dergisi 7(3): 30-39.
- Meral, R., (2017). Farklı Sıcaklık derecelerinin uşkun bitkisinin antioksidan aktivitesi ve fenolik profili üzerine etkisi. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 27(1), 88-94.
- Mohammed, B., and Karim., J. K., (2010). Antitumoractivity of Rheum ribes and Thymus syriacus in male albino mice, Journal of Thi-Qar Science, 2(3), 2-11.
- Mohammed, I. H., Kakey, E. S., and Farimani, M. M., (2018). In vitro evaluation of antioxidant activates for parts of rhubarb (Rheum ribes) and syrian mesquite (Prosopis farcta). International Conference on Pure and Applied Sciences (ICPAS 2018)
- Nemat Shahi, M. M., Elhami Rad, A. H., Nemat Shahi, N., and Bakhsh Amin, M. R., (2016). Study of antioxidant activity and free radical scavenging power of Rheum ribes flower extract. Journal of Fundamental and Applied Sciences, 8(3), 1164-1174.
- Nishikimi, M., Rao, Na., and Yagi, K., (1972). The occurance of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochemical and Biophysical Research Communications, 46(2), 849-854.
- Obaida, H. H., Tawfeeqb, H. K., Zamel Khalafc, Z., and Shafeeq, Z. S., (2016). Inhibitory effect of rhizomes methanolic extracts of Rheum ribes and TiO2 NPs on Escherichia coli. International Journal of Sciences: Basic and Applied Research (IJSBAR), 30(1), 262-275.
- Oktay, M., Yıldırım, A., Bilaloglu, V., and Gulcin I., (2007). Antioxidant activity of different parts of ısgin (Rheum ribes L.), Asian Journal of Chemistry, 19(4), 3047-3055.
- Oyaizu, M., (1986). Studies on product of browing reaction prepared from glucose amine. Japan of Nutrition, 44, 307-315.
- Ozturk, M., Aydogmus Ozturk, F., Duru, M. E., and Topcu, G., (2007). Antioxidant activity of stem and root extracts of Rhubarb (Rheum ribes): An edible medicinal plant. Food Chemistry,103(2), 623-630.
- Samancioglu, A., Sat, I. G., Yildirim, E., Ercisli, S., Jurikova, T., and Mlcek, T., (2016). Total phenolic and vitamin C content and antiradical activity evaluation of traditionally consumed wild edible vegetables from Turkey. Indian Journal of Traditional Knowledge, 15(2), 208-213.
- Sharm, S., and Vig, P. A., (2013). Evaluation of in vitro antioxidant properties of methanol and aqueous extracts of Parkinsonia aculeata L. leaves. The Scientific World Journal, 2013. Article ID 604865
- Stankovic, M. S., (2011). Total phenolic content,flavonoid concentration and antioxdant activity of Marrubium peregrinum L. extracts, Kragujevac Journal of Science. 33(2011), 63-72.
- Tanis, H., Karcioğlu, L., Dıraz, E., ve Aygan, A., (2010). Kahramanmaras Bölgesinde Yetisen Isgın (Rheum ribes L.)’ın Antibakteriyal Aktivitesinin Belirlenmesi. KSÜ Doğa Bilimleri Dergisi, 13(2), 1-3.
- Tanrikut, S. E., Ceken, B., Altas, S., Pirinccioglu, M., Kizil, G., and Kizil, M., (2013). DNA cleavage protecting activity and in vitro antioxidant potential of aqueous extract from fresh stems of Rheum ribes. Acta Alimentaria, 42(4), 461-472.
- Tosun, F., and Akyuz Kızılay, C., (2003). Anthraquinones and flavanoids from Rheum ribes. Journal of Faculty Pharmacy Ankara, 32(1), 31-35.
- Yildirim, I., Kutlu, T., and Takim, K., (2015). Comparison of antioxidant activity of Rheum ribes fruits and seed methanolic extracts against protein oxidation and lipid peroxidation. Pakistan Journal of Biological Sciences, 18(5), 232-239.
Year 2021,
Volume: 11 Issue: 1, 104 - 117, 15.06.2021
Abstract
Polygonaceae familyasının türü olan Rheum ribes’in taze gövde ve yaprakları Lübnan, İran ve Türkiye’nin doğusunda sebze olarak tüketilmektedir. Son zamanlarda farmakolojik alanlarda kullanılan bu bitkiden Asya ülkelerinde saf ilaç materyalleri üretilmektedir. Çalışmamızda, Rheum ribes gövde kısımlarından etil asetat, hekzan, metanol ve saf su çözücüleri kullanılarak 10-100 mg/mL konsantrasyonlarda hazırlanan özütlerin in vitro fitokimyasal, antioksidan ve antibakteriyel aktiviteleri incelenmiştir. Çözücülere bağlı olarak ekstraksiyon verimi değişmekle birlikte en yüksek ekstraksiyon verimi (%25.3), en yüksek total fenolik (0.80±0.00 mg GAE/g) ve flavonoid içerik (0.321±0.00 mg RE/g) 100 mg/mL konsantrasyonda metanol özütünde belirlenmiştir. Rheum ribes metanol gövde özütünün DPPH· süpürme aktivitesi %91.10±0.51 olarak kaydedilmiş olup IC50 dozu 10.50 mg/mL’dir. En yüksek Fe(II) iyonlarını şelatlama aktivitesi %49.70±3.10, indirgeme kapasitesi (0.19±0.00) ve süperoksit anyonu giderme aktivitesi (%39.20±2.40) 100 mg/mL konsantrasyonda metanol özütünde belirlenmiştir. Rheum ribes’in etil asetat, hekzan ve metanol özütlerinin test bakterileri üzerinde antibakteriyel aktivite gösteremediği belirlenmiştir. Saf su özütünün gösterdiği antibakteriyel aktivite 9.00±1.00 ve 14.50±1.50 mm zon çapı aralığındadır. En yüksek inhibitör etki 14.50±1.50 mm zon çapı ile S. aureus’a karşı gözlenmiştir.
Keywords
References
- Andiç, S., Tuncturk, Y., Ocak, E., and Kose, S., (2009). Some chemica characteristic of edible wild rhubarb species (Rheum ribes L.). Research Journal of Agriculture and Biological Sciences, 5(6), 973-977.
- Bauer, A. W., Kirby, W. M., Sherris, J. C., and Turck, M., (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45(4), 493-496.
- Blois, M. S., (1958). Antioxidant determinations by the use of a stable free radical. Nature, 26, 1199-1200.
- Davis, P. H., (1967). Flora of Turkey and The Aegean Islands, Edinburg Universiyt Press
- Dinis, T. C., Madeira, V. M., and Almeida, L. M., (1994). Action of phenolic derivates (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics, 315(1), 161-169.
- Güvenç, İ., Kaya, Y., (1996). Erzurum'da sebze olarak değerlendirilen yöresel bazı bitkiler. Ankara Üniversitesi Ziraat Fakültesi Dergisi, 27(3): 369-374.
- Khalida, K. A., Ehklass, M. T., and Saleh, R. S., (2014). Phenolic profile, antioxidant, and antibacterial effects of ethanol and aqueous extracts of Rheum ribes L. Roots, Der Pharmacia Lettre, 6(5), 201-205.
- Kosikowska, U., Smolarz, H. D., and Malm, A., (2010). Antimicrobial activity and total content of polyphenols of Rheum L. species growing in Poland, Current Europan Journal of Biology, 5(6), 814-820.
- Memişoğulları, R., 2005. Diyabette serbest radikallerin rolü ve antioksidanların etkisi. Düzce Tıp Fakültesi Dergisi 7(3): 30-39.
- Meral, R., (2017). Farklı Sıcaklık derecelerinin uşkun bitkisinin antioksidan aktivitesi ve fenolik profili üzerine etkisi. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 27(1), 88-94.
- Mohammed, B., and Karim., J. K., (2010). Antitumoractivity of Rheum ribes and Thymus syriacus in male albino mice, Journal of Thi-Qar Science, 2(3), 2-11.
- Mohammed, I. H., Kakey, E. S., and Farimani, M. M., (2018). In vitro evaluation of antioxidant activates for parts of rhubarb (Rheum ribes) and syrian mesquite (Prosopis farcta). International Conference on Pure and Applied Sciences (ICPAS 2018)
- Nemat Shahi, M. M., Elhami Rad, A. H., Nemat Shahi, N., and Bakhsh Amin, M. R., (2016). Study of antioxidant activity and free radical scavenging power of Rheum ribes flower extract. Journal of Fundamental and Applied Sciences, 8(3), 1164-1174.
- Nishikimi, M., Rao, Na., and Yagi, K., (1972). The occurance of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochemical and Biophysical Research Communications, 46(2), 849-854.
- Obaida, H. H., Tawfeeqb, H. K., Zamel Khalafc, Z., and Shafeeq, Z. S., (2016). Inhibitory effect of rhizomes methanolic extracts of Rheum ribes and TiO2 NPs on Escherichia coli. International Journal of Sciences: Basic and Applied Research (IJSBAR), 30(1), 262-275.
- Oktay, M., Yıldırım, A., Bilaloglu, V., and Gulcin I., (2007). Antioxidant activity of different parts of ısgin (Rheum ribes L.), Asian Journal of Chemistry, 19(4), 3047-3055.
- Oyaizu, M., (1986). Studies on product of browing reaction prepared from glucose amine. Japan of Nutrition, 44, 307-315.
- Ozturk, M., Aydogmus Ozturk, F., Duru, M. E., and Topcu, G., (2007). Antioxidant activity of stem and root extracts of Rhubarb (Rheum ribes): An edible medicinal plant. Food Chemistry,103(2), 623-630.
- Samancioglu, A., Sat, I. G., Yildirim, E., Ercisli, S., Jurikova, T., and Mlcek, T., (2016). Total phenolic and vitamin C content and antiradical activity evaluation of traditionally consumed wild edible vegetables from Turkey. Indian Journal of Traditional Knowledge, 15(2), 208-213.
- Sharm, S., and Vig, P. A., (2013). Evaluation of in vitro antioxidant properties of methanol and aqueous extracts of Parkinsonia aculeata L. leaves. The Scientific World Journal, 2013. Article ID 604865
- Stankovic, M. S., (2011). Total phenolic content,flavonoid concentration and antioxdant activity of Marrubium peregrinum L. extracts, Kragujevac Journal of Science. 33(2011), 63-72.
- Tanis, H., Karcioğlu, L., Dıraz, E., ve Aygan, A., (2010). Kahramanmaras Bölgesinde Yetisen Isgın (Rheum ribes L.)’ın Antibakteriyal Aktivitesinin Belirlenmesi. KSÜ Doğa Bilimleri Dergisi, 13(2), 1-3.
- Tanrikut, S. E., Ceken, B., Altas, S., Pirinccioglu, M., Kizil, G., and Kizil, M., (2013). DNA cleavage protecting activity and in vitro antioxidant potential of aqueous extract from fresh stems of Rheum ribes. Acta Alimentaria, 42(4), 461-472.
- Tosun, F., and Akyuz Kızılay, C., (2003). Anthraquinones and flavanoids from Rheum ribes. Journal of Faculty Pharmacy Ankara, 32(1), 31-35.
- Yildirim, I., Kutlu, T., and Takim, K., (2015). Comparison of antioxidant activity of Rheum ribes fruits and seed methanolic extracts against protein oxidation and lipid peroxidation. Pakistan Journal of Biological Sciences, 18(5), 232-239.
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Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | June 15, 2021 |
| Published in Issue | Year 2021 Volume: 11 Issue: 1 |
Cited By
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