Evaluation of Free Radical Scavenging, Anti-inflammatory and Hypoglycemic Activity of Helianthemum oelandicum subsp. incanum (Willk.) G. Lopez from Türkiye
Abstract
Helianthemum is represented by 19 taxa, and out of four are endemic in Türkiye. The antioxidant, anti-inflammatory, and hypoglycemic activities of the ethanol (75%) and aqueous extracts from aerial parts of Helianthemum oelandicum subsp. incanum growing in Türkiye was examined in this study. The water extract exhibited antioxidant activity with 67.26±0.49 and 68.08±0.41 µg ml-1 values on DPPH and ABTS radicals, respectively. The antioxidant activity value of ethanol extract on DPPH radical was found as 46.71±0.27 µg ml-1, where as this value was determined as 51.60±0.76 µg ml-1 for scavenging effects on ABTS radical. Both ethanol and aqueous extracts possessed in vitro anti-inflammatory activity by inhibiting erythrocyte membrane hemolysis in a concentration dependent manner and ethanol extracts exhibited greater activity than aqueous extracts. By measuring the inhibitory effect of the extracts on α-glucosidase, in vitro hypoglycemic activity was assessed. According to the results of this study, the IC50 values of both ethanolic and aqueous extracts were found to be close to each other, 2.52±0.01 and 3.21±0.01 µg ml-1, respectively and compared with the standard compound acarbose (IC50= 0.90±0.01 µg ml-1) it was determined that both extracts exhibited strong inhibition on α-glucosidase. Based on all results evaluated, the ethanolic extract displayed higher antioxidant, anti-inflammatory, and hypoglycemic activities than the aqueous extract.
Keywords
Free radical scavenging activity Anti-inflammatory activity Hypoglycemic activity Helianthemum oelandicum subsp. incanum
Thanks
The authors would like to thank to Prof. Dr. Hayri Duman for identification of the plant.
References
- Anokwuru, C.P., Anyasor, G.N., Ajibaye, O., Fakoya, O. & Okebugwu, P. (2011). Effect of extraction solvents on phenolic, flavonoid and antioxidant activities of three nigerian medicinal plants. Natural and Science, 9(7), 53-61.
- Baldemir, A., Gokşen, N., Ildız, N., Şeker Karatoprak, G. & Koşar, M. (2017). Phytochemical profile and biological activities of Helianthemum canum L. BAUMG. from Turkey. Chemistry & Biodiversity, 14, 2-12.
- Blois, M.S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 26, 1199-1200. https://doi.org/10.1002/cbdv.201700052
- Barbosa, E., Calzada, F. & Campos, R (2006). Antigiardial Activity of Methanolic Extracts from Helianthemum glomeratum Lag. and Rubus coriifolius Focke in Suckling Mice CD-1. Journal of Ethnopharmacology 108, 395–397. https://doi.org/ 10.1016/j.jep.2006.05.026
- Baytop, T. (1999). Türkiye’de Bitkilerle Tedavi (Therapy with Medicinal Plants in Turkey), Nobel Tıp Basımevi, İstanbul.
- Benabdelaziz, I., Marcourt, L., Benkhaled, M., Wolfender, J.L. & Haba, H (2017). Antioxidant and antibacterial activities and polyphenolic constituents of Helianthemum sessiliflorum Pers. Natural Product Research, 31(6), 686-690. https://doi.org/10.1080/14786419.2016.1209669
- Calzada, F. & Alanis, A.D. (2007). Additional antiprotozoal flavonol glycosides of the aerial parts of Helianthemum glomeratum. Phytotherapy Research, 21, 78-80. doi: 10.1002/ptr.2031
- Chemam, Y., Benayache, S., Marchioni, E., Zhao, M., Mosset, P. & Benayache, F (2017). On-Line screening, isolation and identification of antioxidant compounds of Helianthemum ruficomum. Molecules, 22(239), 1-14. https://doi. org/10.3390/molecules22020239
- Davis, P.H. (1965). Flora of Turkey and East Aegean Islands, Vol I, Edinburgh University Press, Edinburgh, UK.
- Demir, T., Akpınar, Ö., Kara, H. & Güngör, H. (2019). Nar (Punica granatum L.) kabuğunun in vitro antidiyabetik, antienflamatuar, sitotoksik, antioksidan ve antimikrobiyal aktivitesi. Akademik Gıda, 17(1), 61-71. https://doi.org/ 10.24323/akademik-gida.544647
- Demir, T., Akpınar, Ö., Kara, H. & Güngör, H. (2020). Cherry stem phenolic compounds: Optimization of extraction conditions and in vitro evaluations of antioxidant, antimicrobial, antidiabetic, anti-inflammatory, and cytotoxic activities. Journal of Food Processing and Preservation, 44, e14804. https://doi.org/10.1111/jfpp.14804
- Djemam, N., Lassed, S., Gül, F., Altun, M., Monteiro, M., Menezes-Pinto, D., Benayache, S., Benayache, F., Zama, D., Demirtas, I. & Morato, M. (2020). Characterization of Ethyl Acetate and n-Butanol Extracts of Cymbopogon schoenanthus and Helianthemum lippii and their effect on the smooth muscle of the rat distal colon. Journal of Ethnopharmacology, 252, 112613. https://doi.org/ 10.1016/j.jep.2020.112613
- Gunathilake, K.D.P.P., Ranaweera, K.K.D.S. & Rupasinghe, H.P. (2018). In vitro anti-inflammatory properties of selected green leafy vegetables. Biomedicines, 6(4), 107. https://doi.org/ 10.3390/ biomedicines6040107
- Hamza, N., Berke, B., Umar, A., Cheze, C., Gin, H. & Moore, N. (2019). A review of algerian medicinal plants used in the treatment of diabetes. Journal of Ethnopharmacology, 238, 1-28. https://doi.org/ 10.1016/j.jep.2019.111841
- Liu, X., Zhu, L., Tan, J., Zhou, X., Xiao, L., Yang, X. & Wang, B. (2014). Glucosidase inhibitory activity and antioxidant activity of flavonoid compound and triterpenoid compound from Agrimonia pilosa Ledeb. BMC Complementary and Alternative Medicine, 14(12), 1-10. https://doi.org/10.1186/ 1472-6882-14-12
- Okur, M.E., Polat, D.C., Ozbek, H., Yilmaz, S., Yoltas, A. & Arslan, R. (2018). Evaluation of the antidiabetic property of capparis ovata desf. var. palaestina zoh. extracts using in vivo and in vitro approaches. Endocrine, Metabolic & Immune Disorders-Drug Targets (Formerly Current Drug Targets-Immune, Endocrine & Metabolic Disorders), 18(5), 489-501. https://doi.org/10.2174/ 1871530318666180328110524.
- Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237. https://doi.org/ 10.1016/s0891-5849(98)00315-3
- Rubio-Moraga, A., Argandoña, J., Mota, B., Pérez, J., Verde, A., Fajardo, J., Gómez-Navarro, J., Castillo-López, R., Ahrazem, O. & Gómez-Gómez, L. (2013). Screening for polyphenols, antioxidant and antimicrobial activities of extracts from eleven Helianthemum taxa (Cistaceae) used in folk medicine in South-Eastern Spain. Journal of Ethnopharmacology, 148, 287-296. https://doi.org/ 10.1016/j.jep.2013.04.028
- Shinde, U., Phadke, A., Nair, A., Mungantiwar, A., Dikshit, V. & Saraf, M. (1999). Membrane stabilizing activity—a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia, 70(3), 251-257. https://doi.org/10.1016/S0367-326X(99)00030-1
- Sultana, B., Anwar, F. & Ashraf, M. (2009). Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules, 14, 2167-2180. https://doi.org/10.3390/ molecules14062167
- Şam Gökşen, N. & Baldemir, A. (2016). Traditional usages of Helianthemum Mıller species. Journal of Health Sciences, 25, 49-52.
- Terfassi, S., Dauvergne, X., Stephane Cerantola, S., Lemoine, C., Bensouici, C., Fadila, B., Christian M, Marchioni, E. & Benayache, S. (2021). First report on phytochemical investigation, antioxidant and antidiabetic activities of Helianthemum getulum. Natural Product Research, 1-8. https://doi.org/10.1080/14786419.2021.1928664
- Yalcin, C.Ö., Yilmaz Sarialtin, S. & Cicek Polat, D. (2020). Quantification of phenolic and flavonoid contents and some biological activities of Ornithogalum sigmoideum Freyn & Sint. Journal of Research in Pharmacy, 24(4), 487-496. https://doi.org/10.35333/jrp.2020.197
Türkiye’de Yetişen Helianthemum oelandicum subsp. incanum (Willk.) G. Lopez Bitkisinin Serbest Radikal Süpürücü, Anti-inflamatuvar ve Hipoglisemik Aktivitelerinin Değerlendirilmesi
Abstract
Helianthemum Türkiye’de 4’ü endemik olmak üzere 19 takson ile temsil edilmektedir. Bu çalışmada Türkiye’de doğal olarak yetişen Helianthemum oelandicum subsp. incanum toprak üstü kısımlarının etanollü (%75) ve sulu ekstrelerinin antioksidan, anti-inflamatuvar ve hipoglisemik aktiviteleri araştırılmıştır. Sulu ekstrenin DPPH ve ABTS radikalleri üzerine gösterdiği süpürücü etki miktarı sırasıyla 67.26±0.49 ve 68.08±0.41 µg ml-1 olarak tespit edilmiştir. Bitkinin etanollü ekstresinin DPPH radikaline karşı gösterdiği antioksidan aktivite değeri 46.71±0.27 µg ml-1 iken, ABTS radikali için bu değer 51.60±0.76 µg ml-1 olarak bulunmuştur. Bitkinin hem etanollü hem de sulu ekstresinin eritrosit membran hemolizini konsantrasyona bağlı olarak inhibe ederek in vitro anti-inflamatuvar etki gösterdiği ve etanollü ekstrenin sulu ekstreye göre daha güçlü etkiye sahip olduğu belirlenmiştir. Bitki ekstrelerinin in vitro hipoglisemik aktivite tayini için α-glukozidaza karşı inhibisyon etkileri ölçülmüş, etanollü ve sulu ekstrelerin IC50 değerleri sırasıyla 2.52±0.01 ve 3.21±0.01 µg ml-1 bulunmuş ve standart bileşik akarboz ile karşılaştırıldığında (IC50= 0.90±0.01 µg ml-1) α-glukozidaza karşı güçlü inhibisyon gösterdikleri tespit edilmiştir. Tüm aktivite sonuçları bir arada değerlendirildiğinde etanollü ekstrenin antioksidan, anti-inflamatuvar ve hipoglisemik aktivitesinin sulu ekstreden daha yüksek olduğu bulunmuştur.
Keywords
Serbest radikal süpürücü aktivite Anti-inflamatuvar aktivite Hipoglisemik aktivite Helianthemum oelandicum subsp. incanum.
References
- Anokwuru, C.P., Anyasor, G.N., Ajibaye, O., Fakoya, O. & Okebugwu, P. (2011). Effect of extraction solvents on phenolic, flavonoid and antioxidant activities of three nigerian medicinal plants. Natural and Science, 9(7), 53-61.
- Baldemir, A., Gokşen, N., Ildız, N., Şeker Karatoprak, G. & Koşar, M. (2017). Phytochemical profile and biological activities of Helianthemum canum L. BAUMG. from Turkey. Chemistry & Biodiversity, 14, 2-12.
- Blois, M.S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 26, 1199-1200. https://doi.org/10.1002/cbdv.201700052
- Barbosa, E., Calzada, F. & Campos, R (2006). Antigiardial Activity of Methanolic Extracts from Helianthemum glomeratum Lag. and Rubus coriifolius Focke in Suckling Mice CD-1. Journal of Ethnopharmacology 108, 395–397. https://doi.org/ 10.1016/j.jep.2006.05.026
- Baytop, T. (1999). Türkiye’de Bitkilerle Tedavi (Therapy with Medicinal Plants in Turkey), Nobel Tıp Basımevi, İstanbul.
- Benabdelaziz, I., Marcourt, L., Benkhaled, M., Wolfender, J.L. & Haba, H (2017). Antioxidant and antibacterial activities and polyphenolic constituents of Helianthemum sessiliflorum Pers. Natural Product Research, 31(6), 686-690. https://doi.org/10.1080/14786419.2016.1209669
- Calzada, F. & Alanis, A.D. (2007). Additional antiprotozoal flavonol glycosides of the aerial parts of Helianthemum glomeratum. Phytotherapy Research, 21, 78-80. doi: 10.1002/ptr.2031
- Chemam, Y., Benayache, S., Marchioni, E., Zhao, M., Mosset, P. & Benayache, F (2017). On-Line screening, isolation and identification of antioxidant compounds of Helianthemum ruficomum. Molecules, 22(239), 1-14. https://doi. org/10.3390/molecules22020239
- Davis, P.H. (1965). Flora of Turkey and East Aegean Islands, Vol I, Edinburgh University Press, Edinburgh, UK.
- Demir, T., Akpınar, Ö., Kara, H. & Güngör, H. (2019). Nar (Punica granatum L.) kabuğunun in vitro antidiyabetik, antienflamatuar, sitotoksik, antioksidan ve antimikrobiyal aktivitesi. Akademik Gıda, 17(1), 61-71. https://doi.org/ 10.24323/akademik-gida.544647
- Demir, T., Akpınar, Ö., Kara, H. & Güngör, H. (2020). Cherry stem phenolic compounds: Optimization of extraction conditions and in vitro evaluations of antioxidant, antimicrobial, antidiabetic, anti-inflammatory, and cytotoxic activities. Journal of Food Processing and Preservation, 44, e14804. https://doi.org/10.1111/jfpp.14804
- Djemam, N., Lassed, S., Gül, F., Altun, M., Monteiro, M., Menezes-Pinto, D., Benayache, S., Benayache, F., Zama, D., Demirtas, I. & Morato, M. (2020). Characterization of Ethyl Acetate and n-Butanol Extracts of Cymbopogon schoenanthus and Helianthemum lippii and their effect on the smooth muscle of the rat distal colon. Journal of Ethnopharmacology, 252, 112613. https://doi.org/ 10.1016/j.jep.2020.112613
- Gunathilake, K.D.P.P., Ranaweera, K.K.D.S. & Rupasinghe, H.P. (2018). In vitro anti-inflammatory properties of selected green leafy vegetables. Biomedicines, 6(4), 107. https://doi.org/ 10.3390/ biomedicines6040107
- Hamza, N., Berke, B., Umar, A., Cheze, C., Gin, H. & Moore, N. (2019). A review of algerian medicinal plants used in the treatment of diabetes. Journal of Ethnopharmacology, 238, 1-28. https://doi.org/ 10.1016/j.jep.2019.111841
- Liu, X., Zhu, L., Tan, J., Zhou, X., Xiao, L., Yang, X. & Wang, B. (2014). Glucosidase inhibitory activity and antioxidant activity of flavonoid compound and triterpenoid compound from Agrimonia pilosa Ledeb. BMC Complementary and Alternative Medicine, 14(12), 1-10. https://doi.org/10.1186/ 1472-6882-14-12
- Okur, M.E., Polat, D.C., Ozbek, H., Yilmaz, S., Yoltas, A. & Arslan, R. (2018). Evaluation of the antidiabetic property of capparis ovata desf. var. palaestina zoh. extracts using in vivo and in vitro approaches. Endocrine, Metabolic & Immune Disorders-Drug Targets (Formerly Current Drug Targets-Immune, Endocrine & Metabolic Disorders), 18(5), 489-501. https://doi.org/10.2174/ 1871530318666180328110524.
- Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237. https://doi.org/ 10.1016/s0891-5849(98)00315-3
- Rubio-Moraga, A., Argandoña, J., Mota, B., Pérez, J., Verde, A., Fajardo, J., Gómez-Navarro, J., Castillo-López, R., Ahrazem, O. & Gómez-Gómez, L. (2013). Screening for polyphenols, antioxidant and antimicrobial activities of extracts from eleven Helianthemum taxa (Cistaceae) used in folk medicine in South-Eastern Spain. Journal of Ethnopharmacology, 148, 287-296. https://doi.org/ 10.1016/j.jep.2013.04.028
- Shinde, U., Phadke, A., Nair, A., Mungantiwar, A., Dikshit, V. & Saraf, M. (1999). Membrane stabilizing activity—a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia, 70(3), 251-257. https://doi.org/10.1016/S0367-326X(99)00030-1
- Sultana, B., Anwar, F. & Ashraf, M. (2009). Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules, 14, 2167-2180. https://doi.org/10.3390/ molecules14062167
- Şam Gökşen, N. & Baldemir, A. (2016). Traditional usages of Helianthemum Mıller species. Journal of Health Sciences, 25, 49-52.
- Terfassi, S., Dauvergne, X., Stephane Cerantola, S., Lemoine, C., Bensouici, C., Fadila, B., Christian M, Marchioni, E. & Benayache, S. (2021). First report on phytochemical investigation, antioxidant and antidiabetic activities of Helianthemum getulum. Natural Product Research, 1-8. https://doi.org/10.1080/14786419.2021.1928664
- Yalcin, C.Ö., Yilmaz Sarialtin, S. & Cicek Polat, D. (2020). Quantification of phenolic and flavonoid contents and some biological activities of Ornithogalum sigmoideum Freyn & Sint. Journal of Research in Pharmacy, 24(4), 487-496. https://doi.org/10.35333/jrp.2020.197
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Publication Date | April 30, 2023 |
| Submission Date | October 27, 2021 |
| Acceptance Date | March 3, 2022 |
| Published in Issue | Year 2023 |
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2022-JCI = 0.170
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