Effects of 6-benzylaminopurine and Activated Carbon on Indirect Organogenesis of Fritillaria Imperialis
Öz
Fritillaria imperialis needs 2-3 years to regenerate from seeds, which makes its production
quite challenging. In this study, effects of three concentrations of
6-Benzylaminopurine (BAP) (0.00, 0.10 and 0.15 mg/L) and three different
amounts (0.0, 3.0 and 3.5 g/L) of activated carbon (AC) on the growth of F.
imperialis plantlets in vitro were determined. The auxins were at
similar concentration (0.01 mg/L of NAA and 0.01 mg/L of IAA) for all
treatments. It has been found that the size and number of bulblets, as well as
root and shoot numbers in regenerated plantlets were increased significantly
(p<0.05) by adding 3.5 g/L of AC in culture medium supplemented with 0.10
mg/L of BAP. Moreover, addition of AC significantly reduced the length of roots
in all doses studied (p<0.05). The supreme concentration of BAP (0.15 mg/L)
decreased the bulblet number in all treatments, however, when supplemented with
3.5 g/L of AC, it was found to raise all the values measured (p<0.05).
Overall, the best growth of the plant parts from callus were achieved with the
highest concentration of AC and 1.5 mg/L of BAP.
Anahtar Kelimeler
Activated charcoal Bulblet Crown imperial Cytokinin Micropropagation
Destekleyen Kurum
Kahramanmaraş Sütçü İmam Üniversitesi Bilimsel Araştırma Projeleri Birimi
Proje Numarası
2017/4-28D
Teşekkür
This work was supported by Scientific Research Projects (BAP) Unit of Kahramanmaras Sutcu Imam University with the project number of 2017/4-28D.
Kaynakça
- Almeida R, Goncalves S, Romano A 2005. In vitro micropropagation of endongered Rhododendron Ponticum L. subsp. Baeticum (Boissier and Reuter) Handel-Mazzetti. Biodiversity Conservation 14(5):1059-1069.
- Anagnostakis S 1974. Haploid plants from anthers of tobacco Enhancement with charcoal. Planta 155(3):281-283.
- Arditti J, Krikorian AD 1996. Orchid micropropagation: the path from laboratory to commercialization and an account of several unprecedented investigators. Botanical Journal of the Linnean Society 122(3):183-241.
- Bhatia P, Bhatia N, Ashwath N 2002. In vitro propagation of Stackhousiabtryonii Bailey (Stackhousiaceae): A rare and serpertine-endermic species of central Queensland Australia. Biodiversity Conservation 11(8):1469-1477.
- Dumas E, Monteuuis O 1995. In vitro rooting of micropropagated shoots from juvenile and mature Pinus pinaster explants: influence of activated charcoal. Plant Cellular Tissue Organ Culture 40(3):231-235.
- Ebrahimie E, Habashy A, Mohammadie-Dehcheshmeh M, Ghannadha M, Ghareyazie B, Yazdi-Amadi B 2006. Direct shoot regeneration from mature embryo as a rapid and genotype-independent pathway in tissue culture of heterogeneous diverse sets of cumin (Cuminum cyminum L.) genotypes. In Vitro Cellular & Developmental Biology-Plant 42(5):455-460.
- Fridborg G, Eriksson T 1975. Effects of Activated Charcoal on Growth and Morphologenesis in Cell Cultures. Physiologia Plantarum. 34(4):306-308.
- Fridborg G, Pedersen M, Eriksson T 1978. The effect of activated charcoal on tissue culture: adsorption of metabolites inhibiting morphogenesis. Physiologia Plantarum 43(2):104-106.
- Gao S, Zhu D, Cai Z, Jiang Y, Xu D 1999. Organ culture of a precious Chinese medicinal plant – Fritillaria unibracteata. Plant Cell Tissue Organ Culture 59(3):197-201.
- George E, Hall M, De Klerk G 2008. Plant tissue culture procedure- background plant propagation by Tissue culture. Springer, England
- Li S, Li P, Lin G, Chan S, Ho Y 2000. Simultaneous determination of seven major isosteroidal alkaloids in bulb of Fritillaria by gas chromatography. Chromatographia 873(2):221-228.
- Li X, Gao W, Jiang Q, Huang L, Liu C 2011. Study on the morphology crystalline structure, and thermal properties of Fritillaria ussuriensis Maxim starch acetates with different degrees of substitution. Starch-Starke 63(1):24-31.
- Nhut D, Van Le, Fukai S, Tanaka M, Van K 2001. Effects of activated charcoal, explant size, explant position and sucrose concentration on plant and shoot regeneration of Lilium longiflorum via young stem culture. Plant Growth Regulation 33(1):59-65.
- Perry L, Metzger J 1980. Medicinal plants of East and Southeast Asia: attributed properties. Economic Botany 34(4):361-361.
- Saeed N, Cömertpay S 2017. Activated charcoal improve growth of F. imperialis propagated by indirect organogenesis. Columella. Agriculture Environ., 4:50-64.
- Staikkidou I, Selby C, Hanks G 2000. Stimulation of in vitro bulblet growth in Galanthus species with sucrose and activated charcoal. Acta Hortic., 725:421-426.Steinits B, Yahel H 1982. In vitro propagation of Narcissus tazetta. Horticulture Science 17(3):333-334.
- Thomas T 2008. The role of activated charcoal in plant tissue culture. Biotechnology Advance 26(6): 618- 631.
- Wang S, Gao W, Chen H, Xiao P 2005. New starches from Fritillaria species medicinal plants. Carbohydrate polymerases 61(1):111-114.
- Ziv M, Lilien-Kipnis H 2000. Bud regeneration from inflorescence explants for rapid propagation of geophytes in vitro. Plant biology and morphogenesis 19(9):845-850.
6-benzilaminopurin ve Aktif Karbonun Fritillaria Imperialis'in Dolaylı Organogenezi Üzerindeki Etkileri
Öz
Fritillaria imperialis türü bitkiler, tohumlarını
yenilemek için iki yıldan fazla bir süreye ihtiyacı duymakta ve bu durum
üretimlerini zorlaştırmaktadır. Bu çalışmada, üç farklı derişimdeki
6-Benzilaminopurinin (BAP) (0.00, 0.10 ve 0.15 mg/L) ve üç farklı miktardaki
(0.0, 3.0 ve 3.5 g/L) aktif karbonun (AC) F.
imperialis bitkilerinin büyümesine etkisi in vitro olarak
incelenmiştir. Tüm muamele grupları için benzer konsantrasyonda oksin (0.01
mg/L NAA ve 0.01 mg/L IAA) kullanılmıştır. 0.10 mg/L BAP ilave edilmiş kültür
ortamlarına 3.5 g/L AC eklenmesinin bitkilerin yumru çap uzunluğu ve sayısının
yanında kök ve sürgün sayılarını istatiksel olarak anlamlı bir şekilde (p<0.05) arttırdığı
gözlenmiştir. Dahası, AC eklenmesi, çalışılan tüm dozlarda kök uzunluklarını
önemli ölçüde azaltmıştır (p<0.05). Yüksek BAP konsantrasyonunun (0.15 mg/L)
çalışılan tüm grupların yumru sayılarını azalttığı, ancak 3.5 g/L AC ile
desteklendiğinde ölçülen tüm değerlerin artışını sağladığı bulunmuştur
(p<0.05). ).
Genel olarak, kallustan bitki kısımlarının en iyi şekilde büyümesi, en yüksek
konsantrasyonda AC ve >1.5 mg/L BAP ile elde
edilmiştir.
Anahtar Kelimeler
Proje Numarası
2017/4-28D
Kaynakça
- Almeida R, Goncalves S, Romano A 2005. In vitro micropropagation of endongered Rhododendron Ponticum L. subsp. Baeticum (Boissier and Reuter) Handel-Mazzetti. Biodiversity Conservation 14(5):1059-1069.
- Anagnostakis S 1974. Haploid plants from anthers of tobacco Enhancement with charcoal. Planta 155(3):281-283.
- Arditti J, Krikorian AD 1996. Orchid micropropagation: the path from laboratory to commercialization and an account of several unprecedented investigators. Botanical Journal of the Linnean Society 122(3):183-241.
- Bhatia P, Bhatia N, Ashwath N 2002. In vitro propagation of Stackhousiabtryonii Bailey (Stackhousiaceae): A rare and serpertine-endermic species of central Queensland Australia. Biodiversity Conservation 11(8):1469-1477.
- Dumas E, Monteuuis O 1995. In vitro rooting of micropropagated shoots from juvenile and mature Pinus pinaster explants: influence of activated charcoal. Plant Cellular Tissue Organ Culture 40(3):231-235.
- Ebrahimie E, Habashy A, Mohammadie-Dehcheshmeh M, Ghannadha M, Ghareyazie B, Yazdi-Amadi B 2006. Direct shoot regeneration from mature embryo as a rapid and genotype-independent pathway in tissue culture of heterogeneous diverse sets of cumin (Cuminum cyminum L.) genotypes. In Vitro Cellular & Developmental Biology-Plant 42(5):455-460.
- Fridborg G, Eriksson T 1975. Effects of Activated Charcoal on Growth and Morphologenesis in Cell Cultures. Physiologia Plantarum. 34(4):306-308.
- Fridborg G, Pedersen M, Eriksson T 1978. The effect of activated charcoal on tissue culture: adsorption of metabolites inhibiting morphogenesis. Physiologia Plantarum 43(2):104-106.
- Gao S, Zhu D, Cai Z, Jiang Y, Xu D 1999. Organ culture of a precious Chinese medicinal plant – Fritillaria unibracteata. Plant Cell Tissue Organ Culture 59(3):197-201.
- George E, Hall M, De Klerk G 2008. Plant tissue culture procedure- background plant propagation by Tissue culture. Springer, England
- Li S, Li P, Lin G, Chan S, Ho Y 2000. Simultaneous determination of seven major isosteroidal alkaloids in bulb of Fritillaria by gas chromatography. Chromatographia 873(2):221-228.
- Li X, Gao W, Jiang Q, Huang L, Liu C 2011. Study on the morphology crystalline structure, and thermal properties of Fritillaria ussuriensis Maxim starch acetates with different degrees of substitution. Starch-Starke 63(1):24-31.
- Nhut D, Van Le, Fukai S, Tanaka M, Van K 2001. Effects of activated charcoal, explant size, explant position and sucrose concentration on plant and shoot regeneration of Lilium longiflorum via young stem culture. Plant Growth Regulation 33(1):59-65.
- Perry L, Metzger J 1980. Medicinal plants of East and Southeast Asia: attributed properties. Economic Botany 34(4):361-361.
- Saeed N, Cömertpay S 2017. Activated charcoal improve growth of F. imperialis propagated by indirect organogenesis. Columella. Agriculture Environ., 4:50-64.
- Staikkidou I, Selby C, Hanks G 2000. Stimulation of in vitro bulblet growth in Galanthus species with sucrose and activated charcoal. Acta Hortic., 725:421-426.Steinits B, Yahel H 1982. In vitro propagation of Narcissus tazetta. Horticulture Science 17(3):333-334.
- Thomas T 2008. The role of activated charcoal in plant tissue culture. Biotechnology Advance 26(6): 618- 631.
- Wang S, Gao W, Chen H, Xiao P 2005. New starches from Fritillaria species medicinal plants. Carbohydrate polymerases 61(1):111-114.
- Ziv M, Lilien-Kipnis H 2000. Bud regeneration from inflorescence explants for rapid propagation of geophytes in vitro. Plant biology and morphogenesis 19(9):845-850.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Ziraat, Veterinerlik ve Gıda Bilimleri |
| Bölüm | ARAŞTIRMA MAKALESİ (Research Article) |
| Yazarlar | |
| Proje Numarası | 2017/4-28D |
| Yayımlanma Tarihi | 30 Nisan 2020 |
| Gönderilme Tarihi | 30 Eylül 2019 |
| Kabul Tarihi | 8 Kasım 2019 |
| Yayımlandığı Sayı | Yıl 2020 |
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2022-JCI = 0.170
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