Effect of Rhizospheres Bacteria Isolated from Kahramanmaraş Pepper Fields Against Phytophthora capsici

Zeynep Hümeyra Ardıç; Mustafa Küsek; Ceyda Ceyhan

doi:10.18016/ksutarimdoga.vi.702589

Araştırma Makalesi

Effect of Rhizospheres Bacteria Isolated from Kahramanmaraş Pepper Fields Against Phytophthora capsici

Yıl 2021, , 70 - 75, 28.02.2021

Zeynep Hümeyra Ardıç , Mustafa Küsek , Ceyda Ceyhan

https://doi.org/10.18016/ksutarimdoga.vi.702589

Cited By: 1

Öz

For the study, 36 soil samples with roots parts were taken from root zone of better grown healthy pepper plants from the pepper fields of Kahramanmaras province. Bacterial isolations were made from collected soil samples. Overall, 713 isolates were obtained from the bacteria colonies based on the morphological characteristics differences. By measuring the radius of inhibition zone, ZHA17 was determined to be most effective isolate. Fifteen isolates were selected from these bacteria in such a way to establish a zone to inhibit the growth of Phytophthora capsici and used in pot experiments under controlled conditions. The isolates were identified as Bacillus pumilus, B. subtilis ss subtilis, Mycobacterium confluentis, M. immunogenum, Paenibacillus castaneae, Pseudomonas fluorescens, P. viridilivida and Tsukamurella paurometabola bacteria species according to Biolog GEN III identification system.

Anahtar Kelimeler

Pepper, Soil Bacteria, Biolog Gen III, Phytophthora capsici, Biological Control

Destekleyen Kurum

KSÜ Bilimsel Araştırma Yönetim Birimi

Proje Numarası

2014/3 16 YLS

Teşekkür

this study was financially supported by Kahramanmaraş Sütçü Imam University, Scientific Research Project Unit by a Grant number 2014/3 16 YLS.

Kaynakça

Abak K, Pitrat M 1981. Biberlerde Kök Boğazı Yanıklığı (Phytophthora capsici Leon) Hastalığına Dayanıklılık Üzerine Bir Araştırma. A.Ü.Z.F. Yıllığı 29(2-3-4):943-947.
Akgül DS, Mirik M 2008. Biocontrol of Phytophthora capsici on Pepper Plants by Bacillus megaterium Strains. Journal of Plant Pathology 90(1):29-34.
Anand R, Chanway CP 2013. Nif Gene Sequence and Arrangement in the Endophytic Diazotroph Paenibacillus polymyxa Strain P2b-2R. Biol Fertil Soils 49:965-970.
Anand R, Grayston S, Chanway C 2013. N-2-Fixation and Seedling Growth Promotion of Lodgepole Pine by Endophytic Paenibacillus polymyxa. Microb Ecol 66:369-374.
Anonymus 2008. Biolog Gen III Microplate Instruction for Use.
Aysan Y, Karatas A, Cınar O 2003. Biological Control of Bacterial Stem Rot Caused by Erwinina chrysanthemi on Tomato. Crop Protection 22:807-811.
Bruzos MM, Campos JM, Chavez PC, Valdivia RM, Vazquez EP 2013. Interaction Among Tsukamurella paurometabola C-924 and Rhizobium leguminosarum biovar phaseoli CFH in Bean Plants. Acta Agronomica 62(1):52-58.
Daafy F, Adam L, Fernando VGD 2003. Comparative Screening of Bacteria for Biological Control of Potato Late Blight (strain US-8), Using in vitro, Detached-leaves, and Whole-plant Testing Systems. Can J Plant Pathol 25:276-284.
Gordon T, Nadjiezko C, Galdanes K, Lewis D, Donnely K 2006. Mycobacterium immunogenum Causes Hypersensitivity Pneumonitis-like Pathology in Mice. Journal Inhalation Toxicology 18(6):449-456.
Guo T, Liao MD 2014. Suppression of Rhizoctonia solani and Induction of Host Plant Resistance by Paenibacillus kribbensis PS04 Towards Controlling of Rice Sheath Blight. Biocontrol Sci Technol 24:116-121.
Hernandez A, Moreira A, Pimentel E, Martinez J, Mena J, Del Toro N 2008. A Rapid Method for Estimating Viability in Desiccated Cells of the Biocontrol Agent Tsukamurella paurometabola C-924. Journal of Rapid Methods & Automation in Microbiology 16:222-229.
Jee HJ, Nam CK, Kim CH 1988. Studies and Biological Control of Phytophthora Blight of Red Pepper.1. Isolation of Antagonists and Evulation of Antagonistic Activity in vitro and in Greenhouse. Korean J Plant Pathol 4(4):305-312.
Kim SG, Khan Z, Jeon YH, Kim YH 2009. Inhibitory Effect of Paenibacillus polymyxa GBR-462 on Phytophtora capsici causing Phytophtora Blight in Chilli Pepper. Journal of Phytopathology 157(6): 329-337.
Kloepper JW, Ryu CM, Zhang SA 2004. Induced Systemic Resistance and Promotion of Plant Growth by Bacillus spp. Phytopathology 94:1259–1266.
Kone D, Csinos AS, Jackson KL, Ji P2009. Evaluation of Systemic Acquired Inducers for Control of Phytophthora capsici on Squash. Crop Protection 28:533–538.
Leonian LH 1922. Stem and Fruit Blight of Pepper Caused by Phytohthora capsici sp. Nov. Phytopathology 12:401-408.
Ling N, Huang Q, Guo S, Shen Q 2011. Paenibacillus polymyxa SQR-21 Systemically Affects Root Exudates of Watermelon to Decrease the Conidial Germination of Fusarium oxysporum f.sp. niveum. Plant Soil 341:485-493.
Ling N, Wang D, Zhu C, Song Y, Yu G, Ran W, Huang Q, Guo S, Shen Q 2014. Response of the Population Size and Community Structure of Paenibacillus spp. to Different Fertilization Regimes in a Long-term Experiment of Red Soil. Plant Soil 383:87-98.
Loots MAM, De Jong MD, Van Solingen D, Wetsteyn JCFM, Faber WR 2006. Chronic Leg Ulcer Caused by Mycobacterium immunogenum. Journal of Travel Medicine 12(6):347-349.
MarinM, WongI, GarciaG, MoranR, BasultoR, PimentelE, Mena J 2013. Actividad Antagónica in vitro de Tsukamurella paurometabola C-924 Frente a Fitopatógenos. Rev ProtecciónVeg 28(2):132-137.
Mirik M 2005. Biberde Bakteriyel Leke Etmeni Xanthomonas axonopodis pv. vesicatoria’nın Tanılanması ve Bitki Büyüme Düzenleyici Rizobakteriler ile Biyolojik Mücadele Olanakları. Ç.Ü. Fen Bilimleri Enstitüsü Bitki Koruma Anabilim Dalı Doktora Tezi,162 sy.
Raupach GS, Kloepper JW 1998. Mixtures of Plant Growth-Promoting Rhizobacteria Enhance Biological Control of Multiple Cucumber Pathogens. Phytopathology 88:1158-1164.
Rosyid TA, Karim R, Adzahan NM, Chazali FM 2011. Antibacterial Activity of Several Malaysian Leaves Extracts on the Spoilage Bacteria of Yellow Alkaline Noodles. African Journal of Microbiology Research 5(8):898- 904.
Selvaraju SB, Khan IUH, Yadav JS 2005. Biocidal Activity of Formaldehyde and Nonformaldehyde Biocides Toward Mycobacterium immunogenum and Pseudomonas fluorescens in Pure and Mixed Suspensions in Synthetic Metalworking Fluid and Saline.Applied And Environmental Microbiology 71(1): 542-546.
Tran H, Ficke A, Asiimwe T, Höfte M, Raaijmakers JM 2007. Role of the Cyclic Lipopeptide Massetolide A in Biological Control of Phytophthora infestans and in Colonization of Tomato Plants by Pseudomonas fluorescens. New Phytologist 175:731–742.
Tsao PH 1983. Factors Affecting Isolation and Quantitation of Phytophthora from Soil. (Phytophthora: Its Biology, Taxonomy, Ecology, and Pathology, American Phytopathological Society ST Paul, MN., USA: Ed. Erwin DC, Bartnicki-Garcia S, Tsao PH, 219-236 sn.
Uppal AK, El Hadrami A, Adam LR, Menuta M, Daafy F 2007. Pathogenic Variability of Verticillium dahliae Isolates from Patato Fields in Manitoba and Screening of Bacteria for their Biocontrol. Can J Plant Pathol 29:141-152.
Zhang S, White TL, Martinez MC, McInroy JA, Kloepper JW, Klassen W 2010. Evaluation of Plant Growth-Promoting Rhizobacteria for Control of Phytophthora Blight on Squash Under Greenhouse Conditions. Biological Control 53:129–135.

Kahramanmaraş Biber Alanlarından İzole Edilen Toprak Bakterilerinin Phytophthora capsici’ye Karşı Etkisinin Belirlenmesi

Yıl 2021, , 70 - 75, 28.02.2021

Zeynep Hümeyra Ardıç , Mustafa Küsek , Ceyda Ceyhan

https://doi.org/10.18016/ksutarimdoga.vi.702589

Cited By: 1

Öz

Kahramanmaraş ilindeki biber ekim alanlarındaki biber bitkilerinden daha iyi gelişmiş sağlıklı bitkiler seçilerek kök bölgelerinden köklerle birlikte 36 toprak örneği alınmıştır. Bu toprak örneklerinden bakteri izolasyonu yapılarak elde edilen bakteri kolonilerinden morfolojik karakter farklılıkları göz önüne alınarak 713 izolat elde edilmiştir. Engelleme zonlarının yarıçapları ölçülmek suretiyle en etkili izolatın ZHA17 olduğu belirlenmiştir. Phytophthora capsici’ye karşı bu bakterilerden engelleme zonu oluşturan 15 izolat seçilerek saksı çalışmasında kullanılmıştır. Saksı çalışmasında ZHA57, ZHA88, ZHA178, ZHA212, ZHA215, ZHA287 ve ZHA579 izolatları kök boğazı yanıklığı hastalığının gelişimini engellemiştir. Biolog Gen III tanılama sistemine göre izolatlar Bacillus pumilus, Bacillus subtilis ss subtilis, Mycobacterium confluentis, Mycobacterium immunogenum, Paenibacillus castaneae, Pseudomonas fluorescens, Pseudomonas viridilivida ve Tsukamurella paurometabola bakteri türleri olduğu belirlenmiştir.

Anahtar Kelimeler

Biber, Toprak Bakterileri, Biolog Gen III, Phytophthora capsici, Biyolojik Kontrol

Proje Numarası

2014/3 16 YLS

Kaynakça

Abak K, Pitrat M 1981. Biberlerde Kök Boğazı Yanıklığı (Phytophthora capsici Leon) Hastalığına Dayanıklılık Üzerine Bir Araştırma. A.Ü.Z.F. Yıllığı 29(2-3-4):943-947.
Akgül DS, Mirik M 2008. Biocontrol of Phytophthora capsici on Pepper Plants by Bacillus megaterium Strains. Journal of Plant Pathology 90(1):29-34.
Anand R, Chanway CP 2013. Nif Gene Sequence and Arrangement in the Endophytic Diazotroph Paenibacillus polymyxa Strain P2b-2R. Biol Fertil Soils 49:965-970.
Anand R, Grayston S, Chanway C 2013. N-2-Fixation and Seedling Growth Promotion of Lodgepole Pine by Endophytic Paenibacillus polymyxa. Microb Ecol 66:369-374.
Anonymus 2008. Biolog Gen III Microplate Instruction for Use.
Aysan Y, Karatas A, Cınar O 2003. Biological Control of Bacterial Stem Rot Caused by Erwinina chrysanthemi on Tomato. Crop Protection 22:807-811.
Bruzos MM, Campos JM, Chavez PC, Valdivia RM, Vazquez EP 2013. Interaction Among Tsukamurella paurometabola C-924 and Rhizobium leguminosarum biovar phaseoli CFH in Bean Plants. Acta Agronomica 62(1):52-58.
Daafy F, Adam L, Fernando VGD 2003. Comparative Screening of Bacteria for Biological Control of Potato Late Blight (strain US-8), Using in vitro, Detached-leaves, and Whole-plant Testing Systems. Can J Plant Pathol 25:276-284.
Gordon T, Nadjiezko C, Galdanes K, Lewis D, Donnely K 2006. Mycobacterium immunogenum Causes Hypersensitivity Pneumonitis-like Pathology in Mice. Journal Inhalation Toxicology 18(6):449-456.
Guo T, Liao MD 2014. Suppression of Rhizoctonia solani and Induction of Host Plant Resistance by Paenibacillus kribbensis PS04 Towards Controlling of Rice Sheath Blight. Biocontrol Sci Technol 24:116-121.
Hernandez A, Moreira A, Pimentel E, Martinez J, Mena J, Del Toro N 2008. A Rapid Method for Estimating Viability in Desiccated Cells of the Biocontrol Agent Tsukamurella paurometabola C-924. Journal of Rapid Methods & Automation in Microbiology 16:222-229.
Jee HJ, Nam CK, Kim CH 1988. Studies and Biological Control of Phytophthora Blight of Red Pepper.1. Isolation of Antagonists and Evulation of Antagonistic Activity in vitro and in Greenhouse. Korean J Plant Pathol 4(4):305-312.
Kim SG, Khan Z, Jeon YH, Kim YH 2009. Inhibitory Effect of Paenibacillus polymyxa GBR-462 on Phytophtora capsici causing Phytophtora Blight in Chilli Pepper. Journal of Phytopathology 157(6): 329-337.
Kloepper JW, Ryu CM, Zhang SA 2004. Induced Systemic Resistance and Promotion of Plant Growth by Bacillus spp. Phytopathology 94:1259–1266.
Kone D, Csinos AS, Jackson KL, Ji P2009. Evaluation of Systemic Acquired Inducers for Control of Phytophthora capsici on Squash. Crop Protection 28:533–538.
Leonian LH 1922. Stem and Fruit Blight of Pepper Caused by Phytohthora capsici sp. Nov. Phytopathology 12:401-408.
Ling N, Huang Q, Guo S, Shen Q 2011. Paenibacillus polymyxa SQR-21 Systemically Affects Root Exudates of Watermelon to Decrease the Conidial Germination of Fusarium oxysporum f.sp. niveum. Plant Soil 341:485-493.
Ling N, Wang D, Zhu C, Song Y, Yu G, Ran W, Huang Q, Guo S, Shen Q 2014. Response of the Population Size and Community Structure of Paenibacillus spp. to Different Fertilization Regimes in a Long-term Experiment of Red Soil. Plant Soil 383:87-98.
Loots MAM, De Jong MD, Van Solingen D, Wetsteyn JCFM, Faber WR 2006. Chronic Leg Ulcer Caused by Mycobacterium immunogenum. Journal of Travel Medicine 12(6):347-349.
MarinM, WongI, GarciaG, MoranR, BasultoR, PimentelE, Mena J 2013. Actividad Antagónica in vitro de Tsukamurella paurometabola C-924 Frente a Fitopatógenos. Rev ProtecciónVeg 28(2):132-137.
Mirik M 2005. Biberde Bakteriyel Leke Etmeni Xanthomonas axonopodis pv. vesicatoria’nın Tanılanması ve Bitki Büyüme Düzenleyici Rizobakteriler ile Biyolojik Mücadele Olanakları. Ç.Ü. Fen Bilimleri Enstitüsü Bitki Koruma Anabilim Dalı Doktora Tezi,162 sy.
Raupach GS, Kloepper JW 1998. Mixtures of Plant Growth-Promoting Rhizobacteria Enhance Biological Control of Multiple Cucumber Pathogens. Phytopathology 88:1158-1164.
Rosyid TA, Karim R, Adzahan NM, Chazali FM 2011. Antibacterial Activity of Several Malaysian Leaves Extracts on the Spoilage Bacteria of Yellow Alkaline Noodles. African Journal of Microbiology Research 5(8):898- 904.
Selvaraju SB, Khan IUH, Yadav JS 2005. Biocidal Activity of Formaldehyde and Nonformaldehyde Biocides Toward Mycobacterium immunogenum and Pseudomonas fluorescens in Pure and Mixed Suspensions in Synthetic Metalworking Fluid and Saline.Applied And Environmental Microbiology 71(1): 542-546.
Tran H, Ficke A, Asiimwe T, Höfte M, Raaijmakers JM 2007. Role of the Cyclic Lipopeptide Massetolide A in Biological Control of Phytophthora infestans and in Colonization of Tomato Plants by Pseudomonas fluorescens. New Phytologist 175:731–742.
Tsao PH 1983. Factors Affecting Isolation and Quantitation of Phytophthora from Soil. (Phytophthora: Its Biology, Taxonomy, Ecology, and Pathology, American Phytopathological Society ST Paul, MN., USA: Ed. Erwin DC, Bartnicki-Garcia S, Tsao PH, 219-236 sn.
Uppal AK, El Hadrami A, Adam LR, Menuta M, Daafy F 2007. Pathogenic Variability of Verticillium dahliae Isolates from Patato Fields in Manitoba and Screening of Bacteria for their Biocontrol. Can J Plant Pathol 29:141-152.
Zhang S, White TL, Martinez MC, McInroy JA, Kloepper JW, Klassen W 2010. Evaluation of Plant Growth-Promoting Rhizobacteria for Control of Phytophthora Blight on Squash Under Greenhouse Conditions. Biological Control 53:129–135.

Toplam 28 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	İngilizce
Konular	Ziraat, Veterinerlik ve Gıda Bilimleri
Bölüm	ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar	Zeynep Hümeyra Ardıç 0000-0002-6195-6351 Mustafa Küsek 0000-0002-6320-5869 Ceyda Ceyhan 0000-0002-9695-281X
Proje Numarası	2014/3 16 YLS
Yayımlanma Tarihi	28 Şubat 2021
Gönderilme Tarihi	13 Mart 2020
Kabul Tarihi	9 Temmuz 2020
Yayımlandığı Sayı	Yıl 2021

Kaynak Göster

APA	Ardıç, Z. H., Küsek, M., & Ceyhan, C. (2021). Effect of Rhizospheres Bacteria Isolated from Kahramanmaraş Pepper Fields Against Phytophthora capsici. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(1), 70-75. https://doi.org/10.18016/ksutarimdoga.vi.702589