Efficacy of Entomopathogenic Fungi, Beauveria bassiana, Isaria fumosorosea and Metarhizium anisopliae Against Green Shield bug, Palomena prasina L. (Heteroptera: Pentatomidae)

Şeyma Yiğit; İslam Saruhan

doi:10.18016/ksutarimdoga.vi.938399

Araştırma Makalesi

Efficacy of Entomopathogenic Fungi, Beauveria bassiana, Isaria fumosorosea and Metarhizium anisopliae Against Green Shield bug, Palomena prasina L. (Heteroptera: Pentatomidae)

Yıl 2022, , 1051 - 1060, 31.10.2022

Şeyma Yiğit , İslam Saruhan

https://doi.org/10.18016/ksutarimdoga.vi.938399

Cited By: 2

Öz

In this study, the entomopathogenic fungi; Beauveria bassiana, Isaria fumosorosea and Metarhizium anisopliae were evaluated against fourth nymphs and adults of the green shield bug, P. prasina. The experiment was conducted both under field and laboratory conditions. Isolates included six Beauveria bassiana, one Metarhizium anisopliae and one Isaria fumosorosea. Isolates were bioassayed against nymphs and adults in both under field and laboratory conditions. Four replications of the bioassays were analyzed and evaluated daily for 14 days. LT50 and LT95 values for the experimented isolates ranged from 4.19 to 7.11 days and from 6.68 to 11.30 days, respectively in laboratory for nymphs, ranged from 4.98 to 7.18 days and from 8.03 to 12.22 days, respectively in laboratory for adults. LT50 and LT95 values for the experimented isolates ranged from 6.69 to 10.70 days and from 10.29 to 17.91 days, respectively in field for nymphs, ranged from 7.29 to 10.70 days and from 11.23 to 17.91 days, respectively in field for adults. It was obvious that BB1/21b (Beauveria bassiana) was the most virulent on adults of P. prasina. As a result, while the effect of the isolates used in the study was high due to controlled conditions in the laboratory, it was low in field conditions.

Anahtar Kelimeler

Palomena prasina, Green shield bug, Hazelnut, Entomopathogens, Biological control

Destekleyen Kurum

OMÜ BAP

Proje Numarası

PYO.ZRT.1901.18.016

Teşekkür

My sincere thanks go to Prof. Dr. Fikret DEMIRCI (Ankara University, Faculty of Agriculture, Department of Plant Protection) and Associate Prof. Dr. Ismail ERPER (Ondokuz Mayıs University, Faculty of Agriculture, Department of Plant Protection). My other heartfelt appreciation goes to Ondokuz Mayıs University’s Scientific Research Project (BAP) (PROJECT NO: PYO.ZRT.1901.18.016) for the financial support.

Kaynakça

Abbott WS 1925. A method of computing the effectiveness of an insecticide. J. econ. Entomol, 18(2): 265-267.
Anonymous 2019a. Fındık raporu, Şubat 2015, Ankara. T.C. Gümrük ve Ticaret Bakanlığı, Kooperatifçilik Genel Müdürlüğü, 29s. http://koop.gtb.gov.tr/data/53319cec487c8eb1e43d 7299/2014%20F%C4%B1nd%C4%B1k%20Raporu.pdf. Accessed 25 April 2020
Anonymous 2019b. 2014 yılı Fındık sektör raporu. Toprak Mahsulleri Ofisi Genel Müdürlüğü, http://www.tmo.gov.tr/Upload/Document/raporlar/2014findiksektorraporu.pdf. Accessed 25 April 2020.
Ansari MA, Butt TM 2012. Susceptibility of different developmental stages of large pine weevil Hylobius abietis (Coleoptera: Curculionidae) to entomopathogenic fungi and effect of fungal infection to adult weevils by formulation and application methods. Journal of Invertebrate Pathology. 111(1):33-40.
Carrillo D, Dunlap CA, Avery PB, Navarrete J, Duncan RE, Jackson MA, Behle RW, Cave RD, Crane J, Rooney AP, Peña JE 2015. Entomopathogenic fungi as biological control agents for the vector of the laurel wilt disease, the redbay ambrosia beetle, Xyleborus glabratus (Coleoptera: Curculionidae). Biological Control. 81:44-50.
Castrillo LA, Griggs MH, Ranger CM, Reding ME, Vandenberg JD 2011. Virulence of commercial strains of Beauveria bassiana and Metarhizium brunneum (Ascomycota: Hypocreales) against adult Xylosandrus germanus (Coleoptera: Curculionidae) and impact on brood. Biological Control, 58: 121-126.
Copping, L.G., 2004. The Manual of Biocontrol Agents. 3rd Ed., The BioPesticide, British Crop Protection Council, Alton, UK, pp. 702
Deshpande, M.V., 1999. Mycopesticide production by fermentation: Potential and challenges. Journal Critical Reviews in Microbiology, 25(3): 229-243.
Erper I, Saruhan I, Akca I, Aksoy HM, Tuncer C 2016. Evaluation of some entomopathogenic fungi for controlling the Green Shield Bug, Palomena prasina L. (Heteroptera: Pentatomidae). Egyptian Journal of Biological Pest Control. 26(3):573-578.
Gindin G, Levinski S, Glazer I, Soroker V 2006. Evaluation of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana against the red palm weevil. Phytoparasitica. 34 (4):370-379.
Goettel MS, Eilenberg J, Glare T 2005. Entomopathogenic fungi and their role in regulation of insect populations. In: Gilbert, L.I., Iatrou, K., Gill, S.S. (eds). Comprehensive Molecular Insect Science. pp. 361-405.
Gouli V, Gouli S, Skinner M, Hamilton G, Kim JS, Parker BL 2012. Virulence of select entomopathogenic fungi to the brown marmorated stink bug, Halyomorpha halys(Stål) (Heteroptera: Pentatomidae). Pest management science. 68(2): 155-157.
Göktürk T 2020. Determination of the Lethal Effects of Some Entomopathogens on Orosanga japonica (Melichar, 1898) (Hemiptera: Ricaniidae). Turk J Agric Res. 7(3): 305-314
Gurulingappa P, Mcgee P, Sword GA 2011. In vitro and in plants compatibility of insecticides and the endophytic entomopathogen, Lecanicillium lecanii. Mycopathologia. 172: 161–168.
Hirsch J, Reineke A 2014. Efficiency of commercial entomopathogenic fungal species against different members of the genus Otiorhynchus (Coleoptera: Curculionidae) under laboratory and semi-field conditions. Journal of Plant Diseases and Protection, 121(5): 211-218.
Kumar S, Sultana R, Yanar, D 2017. Application of entomopathogenic fungi for insect pests control. Journal of Entomology and Zoology Studies, 5(6): 07-13.
Kushiyev R, Tuncer C, Erper I, Saruhan I 2017. Effectiveness of entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana against Anisandrus dispar (Coleoptera: Curculionidae: Scolytinae). ıx. International congress on Hazelnut. 152 p, 15-19.
Liu J, Zhang Y, Liu T, Tuncer C, Cheng Y 2017. Screening of a highly pathogenic strain against Hazelnut weevil and microscopic observation on its infection process. Journal of Beijing Forestry University. 39(3): 32-37.
Meng, X., Hu, J., Ouyang, G. (2017) The isolation and identification of pathogenic fungi from Tessaratoma papillosa Drury (Hemiptera: Tessaratomidae). Peerj, 5: 1-14 https://doi.org/ 10.7717/peerj.3888
Mustu M, Demirci F, Kocak E 2011. Mortality effects of Isaria farinosa (Holm.) and Beauveria bassiana (Balsamo) Vuillemin (Sordariomycetes: Hypocreales) on Aelia rostrata Boh. (Hemiptera:Pentatomidae). Turkish Journal of Entomology. 35(4): 559-568.
Pike TJ 2014. Interactıons Between The Invasıve Brown Marmorated Stınk Bug, Halyomorpha halys (Hemıptera: Pentatomıdae), And Entomopathogenıc Fungı. Thesis submitted to the Faculty of the Graduate School of the University of Maryland, College Park, in partial fulfillment of the requirements for the degree of Master of Science. P 58.
Prazak RA 1991. Studies on indirect infectionof Trypodendron lineatum Oliv with Beauveria bassiana (Bals.) Vuill. Zeitschrift for Angewandte Entomologie. 111: 431–441.
Romero GR, Antúnez C, Sarubbi-Orue H, Garrido-Jurado B, Valverde-García P, Schade M, Popo TM (2020). Virulence of Metarhizium brunneum (Ascomycota: Hypocreales) Strains Against Stinkbugs Euschistus heros and Dichelops furcatus (Hemiptera: Pentatomidae). Journal of Economic Entomology, 113 (5): 2540–2545
Roy HE, Majerus MEN 2006. Harmonia axyridis: A successful biocontrol agent or an invasive threat? In: Eilenberg J, Hokkanen HMT (Eds) An ecological and societal approach to biological control. Dordrecht, The Netherlands: Kluwer Academic Publishers. 295–309
Samuels RI, Coracini DLA, Dos Santos C M, Gava CAT 2002. Infection of Blissus antillus (Hemiptera: Lygaeidae) eggs by the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana. Biological control, 23(3): 269-273.
Sandhu SS, Sharma AK, Beniwal V, Goel G, Batra P, Kumar A, Jaglan S, Sharma AK and Malhotra S 2012. Myco-Biocontrol of Insect Pests: Factors Involved, Mechanism, and Regulation. Journal of Pathogens. Article ID:126819. http://dx.doi.org/ 10.1155/ 2012/126819.
Saruhan I, Tuncer C 2010. Research of damage rate and type of green shield bug (Palomena prasina L. Heteroptera: Pentatomidae) of hazelnut. Anadolu Journal of Agricultural Sciences. 25(2): 75-83.
Saruhan I, Erper I, Tuncer C, Akca I 2015. Efficiency of some entomopathogenic fungi as biocontrol agents against Aphis fabae Scopoli (Hemiptera: Aphididae). Pak. J. Agri. Sci, 52(2): 273-278.
Shah PA, Pell JK 2003. Entomopathogenic fungi as biological control agents. Applied microbiology and biotechnology. 61(5-6): 413-423.
Tavella L, Arzone A, Miaja ML, Sonnati C 2001. Influence of Bug (Heteroptera, Coreidae and Pentatomidae) feeding activity on hazelnut in Northwest Italy. Acta Hort.556: 461-468.
Tuncer C, Saruhan I, Akça I 2004. The Insect Pest Problem Affecting Hazelnut Kernel Quality in Turkey. 6. International Hazelnut Congress. Tarragona, Spain ActaHort., p. 88.
Tuncer C, Saruhan I, Akça I 2005. The Insect Pest Problem Affecting Hazelnut Kernel Quality in Turkey. Acta Hort. 668: 367-376.
Tuncer C, Kushiyev R, Saruhan I, Erper I 2016. Determination of the effectiveness of entomopathogenic fungi Metarhiziumanisopliae and Beauveria bassiana against Xylosandrus germanus (Coleoptera: Curculionidae: Scolytinae). Turkey 6th Plant Protection Congress with International Participation. p 127.
Wraight, S.P., Jackson, M.A., Kock, S.L., 2001. Production, stabilization and formulation of fungal biocontrol agents. In: T.M. Butt, C. Jackson and N. Magan (Eds.), Fungi as biocontrol agents: Progress problems and potential, CABI Publishing, pp. 253- 287.
Zimmermann G 2008. The entomopathogenic fungi Isaria farinosa (formerly Paecilomyces farinosus) and the Isaria fumosorosea species complex (formerly Paecilomyces fumosoroseus): biology, ecology and use in biological control. Biocont Sci Technol.18: 865–901.

Entomopatojenik funguslar; Beauveria bassiana, Isaria fumosorosea ve Metarhizium anisopliae’nın Palomena prasina L. (Heteroptera: Pentatomidae)’ya karşı etkileri

Yıl 2022, , 1051 - 1060, 31.10.2022

Şeyma Yiğit , İslam Saruhan

https://doi.org/10.18016/ksutarimdoga.vi.938399

Cited By: 2

Öz

Bu çalışmada Beauveria bassiana‘’nın altı farklı ırkı), Isaria (Paecilomyces) fumosorosea ve Metarhizium anisopliae izolatlarının birer ırkı Palomena prasina L. (Heteroptera: Pentatomidae)’nın nimf ve erginlerine karşı laboratuvar (25±1 0C, % 70 nem ve 16:8 h (ışıklı: karanlık) ve arazi koşullarında biyolojik etkinlik denemeleri yapılmıştır. Ayrıca bazı izolatların laboratuvar şartlarında P. prasina’nın yumurtaları üzerine etkinliği belirlenmiştir. Laboratuvar çalışmalarında izolatların 1x108 konidi/mL spor yoğunluğu, 5’er adet ergin ve nimf kullanılmış ve çalışma 4 tekerrürlü olarak yürütülmüştür. Çalışma 14 gün boyunca takip edilerek yüzde ölüm oranları, LT50 ve LT95 değerleri belirlenmiştir. Çalışma sonucunda P. prasina’nın nimflerine karşı laboratuvar şartlarında kullanılan izolatların LT50 ve LT95 değerlerine bakıldığında sırasıyla; 4.19 - 7.11 gün ile 6.68 - 11.30 gün arasında bulunmuştur. Erginlerde ise LT50 ve LT95 değerlerine bakıldığında sırasıyla; 4.98 - 7.18 gün ve 8.03- 12.22 gün arasında belirlenmiştir. Arazi şartlarında ise nimflere karşı yapılan çalışmada izolatların LT50 ve LT95 değerlerine bakıldığında sırasıyla; 6.69 - 10.70 gün ve 10.29 -17.91 gün olarak belirlenmiştir. Erginlerde ise LT50 ve LT95 değerleri 7.29 - 10.70 gün ve 11.23 - 17.91 gün arasında değişmektedir. Yapılan çalışmada kullanılan tüm izolatların P. prasina ergin ve nimflerine karşı arazi şartlarındaki etkinliği labaoratuvar şartlarına nazaran daha düşük olmuştur. Sonuç olarak çalışmada kullanılan tüm izolatların P. prasina’ın ergin ve nimflerine karşı biyolojik mücadele de kullanılma potansiyeli sahip olduğu ortaya konulmuştur.

Anahtar Kelimeler

Yeşil Kokarca, Palomena prasina, Fındık, Entomopatojen fungus, Biyolojik Mücadele

Proje Numarası

PYO.ZRT.1901.18.016

Kaynakça

Abbott WS 1925. A method of computing the effectiveness of an insecticide. J. econ. Entomol, 18(2): 265-267.
Anonymous 2019a. Fındık raporu, Şubat 2015, Ankara. T.C. Gümrük ve Ticaret Bakanlığı, Kooperatifçilik Genel Müdürlüğü, 29s. http://koop.gtb.gov.tr/data/53319cec487c8eb1e43d 7299/2014%20F%C4%B1nd%C4%B1k%20Raporu.pdf. Accessed 25 April 2020
Anonymous 2019b. 2014 yılı Fındık sektör raporu. Toprak Mahsulleri Ofisi Genel Müdürlüğü, http://www.tmo.gov.tr/Upload/Document/raporlar/2014findiksektorraporu.pdf. Accessed 25 April 2020.
Ansari MA, Butt TM 2012. Susceptibility of different developmental stages of large pine weevil Hylobius abietis (Coleoptera: Curculionidae) to entomopathogenic fungi and effect of fungal infection to adult weevils by formulation and application methods. Journal of Invertebrate Pathology. 111(1):33-40.
Carrillo D, Dunlap CA, Avery PB, Navarrete J, Duncan RE, Jackson MA, Behle RW, Cave RD, Crane J, Rooney AP, Peña JE 2015. Entomopathogenic fungi as biological control agents for the vector of the laurel wilt disease, the redbay ambrosia beetle, Xyleborus glabratus (Coleoptera: Curculionidae). Biological Control. 81:44-50.
Castrillo LA, Griggs MH, Ranger CM, Reding ME, Vandenberg JD 2011. Virulence of commercial strains of Beauveria bassiana and Metarhizium brunneum (Ascomycota: Hypocreales) against adult Xylosandrus germanus (Coleoptera: Curculionidae) and impact on brood. Biological Control, 58: 121-126.
Copping, L.G., 2004. The Manual of Biocontrol Agents. 3rd Ed., The BioPesticide, British Crop Protection Council, Alton, UK, pp. 702
Deshpande, M.V., 1999. Mycopesticide production by fermentation: Potential and challenges. Journal Critical Reviews in Microbiology, 25(3): 229-243.
Erper I, Saruhan I, Akca I, Aksoy HM, Tuncer C 2016. Evaluation of some entomopathogenic fungi for controlling the Green Shield Bug, Palomena prasina L. (Heteroptera: Pentatomidae). Egyptian Journal of Biological Pest Control. 26(3):573-578.
Gindin G, Levinski S, Glazer I, Soroker V 2006. Evaluation of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana against the red palm weevil. Phytoparasitica. 34 (4):370-379.
Goettel MS, Eilenberg J, Glare T 2005. Entomopathogenic fungi and their role in regulation of insect populations. In: Gilbert, L.I., Iatrou, K., Gill, S.S. (eds). Comprehensive Molecular Insect Science. pp. 361-405.
Gouli V, Gouli S, Skinner M, Hamilton G, Kim JS, Parker BL 2012. Virulence of select entomopathogenic fungi to the brown marmorated stink bug, Halyomorpha halys(Stål) (Heteroptera: Pentatomidae). Pest management science. 68(2): 155-157.
Göktürk T 2020. Determination of the Lethal Effects of Some Entomopathogens on Orosanga japonica (Melichar, 1898) (Hemiptera: Ricaniidae). Turk J Agric Res. 7(3): 305-314
Gurulingappa P, Mcgee P, Sword GA 2011. In vitro and in plants compatibility of insecticides and the endophytic entomopathogen, Lecanicillium lecanii. Mycopathologia. 172: 161–168.
Hirsch J, Reineke A 2014. Efficiency of commercial entomopathogenic fungal species against different members of the genus Otiorhynchus (Coleoptera: Curculionidae) under laboratory and semi-field conditions. Journal of Plant Diseases and Protection, 121(5): 211-218.
Kumar S, Sultana R, Yanar, D 2017. Application of entomopathogenic fungi for insect pests control. Journal of Entomology and Zoology Studies, 5(6): 07-13.
Kushiyev R, Tuncer C, Erper I, Saruhan I 2017. Effectiveness of entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana against Anisandrus dispar (Coleoptera: Curculionidae: Scolytinae). ıx. International congress on Hazelnut. 152 p, 15-19.
Liu J, Zhang Y, Liu T, Tuncer C, Cheng Y 2017. Screening of a highly pathogenic strain against Hazelnut weevil and microscopic observation on its infection process. Journal of Beijing Forestry University. 39(3): 32-37.
Meng, X., Hu, J., Ouyang, G. (2017) The isolation and identification of pathogenic fungi from Tessaratoma papillosa Drury (Hemiptera: Tessaratomidae). Peerj, 5: 1-14 https://doi.org/ 10.7717/peerj.3888
Mustu M, Demirci F, Kocak E 2011. Mortality effects of Isaria farinosa (Holm.) and Beauveria bassiana (Balsamo) Vuillemin (Sordariomycetes: Hypocreales) on Aelia rostrata Boh. (Hemiptera:Pentatomidae). Turkish Journal of Entomology. 35(4): 559-568.
Pike TJ 2014. Interactıons Between The Invasıve Brown Marmorated Stınk Bug, Halyomorpha halys (Hemıptera: Pentatomıdae), And Entomopathogenıc Fungı. Thesis submitted to the Faculty of the Graduate School of the University of Maryland, College Park, in partial fulfillment of the requirements for the degree of Master of Science. P 58.
Prazak RA 1991. Studies on indirect infectionof Trypodendron lineatum Oliv with Beauveria bassiana (Bals.) Vuill. Zeitschrift for Angewandte Entomologie. 111: 431–441.
Romero GR, Antúnez C, Sarubbi-Orue H, Garrido-Jurado B, Valverde-García P, Schade M, Popo TM (2020). Virulence of Metarhizium brunneum (Ascomycota: Hypocreales) Strains Against Stinkbugs Euschistus heros and Dichelops furcatus (Hemiptera: Pentatomidae). Journal of Economic Entomology, 113 (5): 2540–2545
Roy HE, Majerus MEN 2006. Harmonia axyridis: A successful biocontrol agent or an invasive threat? In: Eilenberg J, Hokkanen HMT (Eds) An ecological and societal approach to biological control. Dordrecht, The Netherlands: Kluwer Academic Publishers. 295–309
Samuels RI, Coracini DLA, Dos Santos C M, Gava CAT 2002. Infection of Blissus antillus (Hemiptera: Lygaeidae) eggs by the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana. Biological control, 23(3): 269-273.
Sandhu SS, Sharma AK, Beniwal V, Goel G, Batra P, Kumar A, Jaglan S, Sharma AK and Malhotra S 2012. Myco-Biocontrol of Insect Pests: Factors Involved, Mechanism, and Regulation. Journal of Pathogens. Article ID:126819. http://dx.doi.org/ 10.1155/ 2012/126819.
Saruhan I, Tuncer C 2010. Research of damage rate and type of green shield bug (Palomena prasina L. Heteroptera: Pentatomidae) of hazelnut. Anadolu Journal of Agricultural Sciences. 25(2): 75-83.
Saruhan I, Erper I, Tuncer C, Akca I 2015. Efficiency of some entomopathogenic fungi as biocontrol agents against Aphis fabae Scopoli (Hemiptera: Aphididae). Pak. J. Agri. Sci, 52(2): 273-278.
Shah PA, Pell JK 2003. Entomopathogenic fungi as biological control agents. Applied microbiology and biotechnology. 61(5-6): 413-423.
Tavella L, Arzone A, Miaja ML, Sonnati C 2001. Influence of Bug (Heteroptera, Coreidae and Pentatomidae) feeding activity on hazelnut in Northwest Italy. Acta Hort.556: 461-468.
Tuncer C, Saruhan I, Akça I 2004. The Insect Pest Problem Affecting Hazelnut Kernel Quality in Turkey. 6. International Hazelnut Congress. Tarragona, Spain ActaHort., p. 88.
Tuncer C, Saruhan I, Akça I 2005. The Insect Pest Problem Affecting Hazelnut Kernel Quality in Turkey. Acta Hort. 668: 367-376.
Tuncer C, Kushiyev R, Saruhan I, Erper I 2016. Determination of the effectiveness of entomopathogenic fungi Metarhiziumanisopliae and Beauveria bassiana against Xylosandrus germanus (Coleoptera: Curculionidae: Scolytinae). Turkey 6th Plant Protection Congress with International Participation. p 127.
Wraight, S.P., Jackson, M.A., Kock, S.L., 2001. Production, stabilization and formulation of fungal biocontrol agents. In: T.M. Butt, C. Jackson and N. Magan (Eds.), Fungi as biocontrol agents: Progress problems and potential, CABI Publishing, pp. 253- 287.
Zimmermann G 2008. The entomopathogenic fungi Isaria farinosa (formerly Paecilomyces farinosus) and the Isaria fumosorosea species complex (formerly Paecilomyces fumosoroseus): biology, ecology and use in biological control. Biocont Sci Technol.18: 865–901.

Toplam 35 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	İngilizce
Konular	Ziraat, Veterinerlik ve Gıda Bilimleri
Bölüm	ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar	Şeyma Yiğit 0000-0003-2268-5103 İslam Saruhan 0000-0003-0229-9627
Proje Numarası	PYO.ZRT.1901.18.016
Yayımlanma Tarihi	31 Ekim 2022
Gönderilme Tarihi	17 Mayıs 2021
Kabul Tarihi	29 Eylül 2021
Yayımlandığı Sayı	Yıl 2022

Kaynak Göster

APA	Yiğit, Ş., & Saruhan, İ. (2022). Efficacy of Entomopathogenic Fungi, Beauveria bassiana, Isaria fumosorosea and Metarhizium anisopliae Against Green Shield bug, Palomena prasina L. (Heteroptera: Pentatomidae). Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(5), 1051-1060. https://doi.org/10.18016/ksutarimdoga.vi.938399