Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

Öz

Plant tissue and organ cultures are important biotechnological methods to produce valuable secondary metabolites such as rosmarinic acid. In this study, It was aimed to determine the rosmarinic acid production possibilities, total phenolic and flavonoid contents and antioxidant activities of adventitious root suspension and solid cultures of basil (Ocimum basilicum L.). The hypocotyl parts of 30-day-old plants grown under in vitro conditions were used as the source of explants for the establishment of adventitious root solid culture. The explants were cultured in dark conditions in nutrient medium containing 3.3 g L-1 MS (Murashige and Skoog), 30 g L-1 sucrose, 2 g L-1 phytagel and 2 mg L-1 indole-3-butyric acid (IBA). Adventitious roots obtained under these conditions were used in the establishment of suspension cultures. Adventitious roots were harvested and analyzed at 10, 20 and 30 days of suspension culture.
The rosmarinic acid content of adventitious roots was analyzed by HPLC. Antioxidant capacities were determined by reducing power (FRAP), free radical scavenging (DPPH) and cation radical scavenging (ABTS) methods. The highest rosmarinic acid content was determined as 32.38 mg g-1 on the 30th day of the adventitious root suspension culture. The highest total phenolic compound content was determined as 32.94 mg GAE g-1 on the 20th day of suspension culture. The highest DPPH, ABTS and FRAP activities were determined on the 30th day of the suspension culture. As a result, It is thought that adventitious roots obtained from suspension cultures of basil plant are suitable materials to produce rosmarinic acid.