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Evaluation of Free Radical Scavenging, Anti-inflammatory and Hypoglycemic Activity of Helianthemum oelandicum subsp. incanum (Willk.) G. Lopez from Türkiye

Year 2023, Volume: 26 Issue: 2, 227 - 233, 30.04.2023

Asli Can Agca Sezen Yılmaz Sarıaltın Betül Sever Yılmaz

https://doi.org/10.18016/ksutarimdoga.vi.1015574
Cited By: 1

Abstract

Helianthemum is represented by 19 taxa, and out of four are endemic in Türkiye. The antioxidant, anti-inflammatory, and hypoglycemic activities of the ethanol (75%) and aqueous extracts from aerial parts of Helianthemum oelandicum subsp. incanum growing in Türkiye was examined in this study. The water extract exhibited antioxidant activity with 67.26±0.49 and 68.08±0.41 µg ml-1 values on DPPH and ABTS radicals, respectively. The antioxidant activity value of ethanol extract on DPPH radical was found as 46.71±0.27 µg ml-1, where as this value was determined as 51.60±0.76 µg ml-1 for scavenging effects on ABTS radical. Both ethanol and aqueous extracts possessed in vitro anti-inflammatory activity by inhibiting erythrocyte membrane hemolysis in a concentration dependent manner and ethanol extracts exhibited greater activity than aqueous extracts. By measuring the inhibitory effect of the extracts on α-glucosidase, in vitro hypoglycemic activity was assessed. According to the results of this study, the IC50 values of both ethanolic and aqueous extracts were found to be close to each other, 2.52±0.01 and 3.21±0.01 µg ml-1, respectively and compared with the standard compound acarbose (IC50= 0.90±0.01 µg ml-1) it was determined that both extracts exhibited strong inhibition on α-glucosidase. Based on all results evaluated, the ethanolic extract displayed higher antioxidant, anti-inflammatory, and hypoglycemic activities than the aqueous extract.

Keywords

Free radical scavenging activity Anti-inflammatory activity Hypoglycemic activity Helianthemum oelandicum subsp. incanum

Thanks

The authors would like to thank to Prof. Dr. Hayri Duman for identification of the plant.

References

  • Anokwuru, C.P., Anyasor, G.N., Ajibaye, O., Fakoya, O. & Okebugwu, P. (2011). Effect of extraction solvents on phenolic, flavonoid and antioxidant activities of three nigerian medicinal plants. Natural and Science, 9(7), 53-61.
  • Baldemir, A., Gokşen, N., Ildız, N., Şeker Karatoprak, G. & Koşar, M. (2017). Phytochemical profile and biological activities of Helianthemum canum L. BAUMG. from Turkey. Chemistry & Biodiversity, 14, 2-12.
  • Blois, M.S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 26, 1199-1200. https://doi.org/10.1002/cbdv.201700052
  • Barbosa, E., Calzada, F. & Campos, R (2006). Antigiardial Activity of Methanolic Extracts from Helianthemum glomeratum Lag. and Rubus coriifolius Focke in Suckling Mice CD-1. Journal of Ethnopharmacology 108, 395–397. https://doi.org/ 10.1016/j.jep.2006.05.026
  • Baytop, T. (1999). Türkiye’de Bitkilerle Tedavi (Therapy with Medicinal Plants in Turkey), Nobel Tıp Basımevi, İstanbul.
  • Benabdelaziz, I., Marcourt, L., Benkhaled, M., Wolfender, J.L. & Haba, H (2017). Antioxidant and antibacterial activities and polyphenolic constituents of Helianthemum sessiliflorum Pers. Natural Product Research, 31(6), 686-690. https://doi.org/10.1080/14786419.2016.1209669
  • Calzada, F. & Alanis, A.D. (2007). Additional antiprotozoal flavonol glycosides of the aerial parts of Helianthemum glomeratum. Phytotherapy Research, 21, 78-80. doi: 10.1002/ptr.2031
  • Chemam, Y., Benayache, S., Marchioni, E., Zhao, M., Mosset, P. & Benayache, F (2017). On-Line screening, isolation and identification of antioxidant compounds of Helianthemum ruficomum. Molecules, 22(239), 1-14. https://doi. org/10.3390/molecules22020239
  • Davis, P.H. (1965). Flora of Turkey and East Aegean Islands, Vol I, Edinburgh University Press, Edinburgh, UK.
  • Demir, T., Akpınar, Ö., Kara, H. & Güngör, H. (2019). Nar (Punica granatum L.) kabuğunun in vitro antidiyabetik, antienflamatuar, sitotoksik, antioksidan ve antimikrobiyal aktivitesi. Akademik Gıda, 17(1), 61-71. https://doi.org/ 10.24323/akademik-gida.544647
  • Demir, T., Akpınar, Ö., Kara, H. & Güngör, H. (2020). Cherry stem phenolic compounds: Optimization of extraction conditions and in vitro evaluations of antioxidant, antimicrobial, antidiabetic, anti-inflammatory, and cytotoxic activities. Journal of Food Processing and Preservation, 44, e14804. https://doi.org/10.1111/jfpp.14804
  • Djemam, N., Lassed, S., Gül, F., Altun, M., Monteiro, M., Menezes-Pinto, D., Benayache, S., Benayache, F., Zama, D., Demirtas, I. & Morato, M. (2020). Characterization of Ethyl Acetate and n-Butanol Extracts of Cymbopogon schoenanthus and Helianthemum lippii and their effect on the smooth muscle of the rat distal colon. Journal of Ethnopharmacology, 252, 112613. https://doi.org/ 10.1016/j.jep.2020.112613
  • Gunathilake, K.D.P.P., Ranaweera, K.K.D.S. & Rupasinghe, H.P. (2018). In vitro anti-inflammatory properties of selected green leafy vegetables. Biomedicines, 6(4), 107. https://doi.org/ 10.3390/ biomedicines6040107
  • Hamza, N., Berke, B., Umar, A., Cheze, C., Gin, H. & Moore, N. (2019). A review of algerian medicinal plants used in the treatment of diabetes. Journal of Ethnopharmacology, 238, 1-28. https://doi.org/ 10.1016/j.jep.2019.111841
  • Liu, X., Zhu, L., Tan, J., Zhou, X., Xiao, L., Yang, X. & Wang, B. (2014). Glucosidase inhibitory activity and antioxidant activity of flavonoid compound and triterpenoid compound from Agrimonia pilosa Ledeb. BMC Complementary and Alternative Medicine, 14(12), 1-10. https://doi.org/10.1186/ 1472-6882-14-12
  • Okur, M.E., Polat, D.C., Ozbek, H., Yilmaz, S., Yoltas, A. & Arslan, R. (2018). Evaluation of the antidiabetic property of capparis ovata desf. var. palaestina zoh. extracts using in vivo and in vitro approaches. Endocrine, Metabolic & Immune Disorders-Drug Targets (Formerly Current Drug Targets-Immune, Endocrine & Metabolic Disorders), 18(5), 489-501. https://doi.org/10.2174/ 1871530318666180328110524.
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237. https://doi.org/ 10.1016/s0891-5849(98)00315-3
  • Rubio-Moraga, A., Argandoña, J., Mota, B., Pérez, J., Verde, A., Fajardo, J., Gómez-Navarro, J., Castillo-López, R., Ahrazem, O. & Gómez-Gómez, L. (2013). Screening for polyphenols, antioxidant and antimicrobial activities of extracts from eleven Helianthemum taxa (Cistaceae) used in folk medicine in South-Eastern Spain. Journal of Ethnopharmacology, 148, 287-296. https://doi.org/ 10.1016/j.jep.2013.04.028
  • Shinde, U., Phadke, A., Nair, A., Mungantiwar, A., Dikshit, V. & Saraf, M. (1999). Membrane stabilizing activity—a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia, 70(3), 251-257. https://doi.org/10.1016/S0367-326X(99)00030-1
  • Sultana, B., Anwar, F. & Ashraf, M. (2009). Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules, 14, 2167-2180. https://doi.org/10.3390/ molecules14062167
  • Şam Gökşen, N. & Baldemir, A. (2016). Traditional usages of Helianthemum Mıller species. Journal of Health Sciences, 25, 49-52.
  • Terfassi, S., Dauvergne, X., Stephane Cerantola, S., Lemoine, C., Bensouici, C., Fadila, B., Christian M, Marchioni, E. & Benayache, S. (2021). First report on phytochemical investigation, antioxidant and antidiabetic activities of Helianthemum getulum. Natural Product Research, 1-8. https://doi.org/10.1080/14786419.2021.1928664
  • Yalcin, C.Ö., Yilmaz Sarialtin, S. & Cicek Polat, D. (2020). Quantification of phenolic and flavonoid contents and some biological activities of Ornithogalum sigmoideum Freyn & Sint. Journal of Research in Pharmacy, 24(4), 487-496. https://doi.org/10.35333/jrp.2020.197

Türkiye’de Yetişen Helianthemum oelandicum subsp. incanum (Willk.) G. Lopez Bitkisinin Serbest Radikal Süpürücü, Anti-inflamatuvar ve Hipoglisemik Aktivitelerinin Değerlendirilmesi

Year 2023, Volume: 26 Issue: 2, 227 - 233, 30.04.2023

Asli Can Agca Sezen Yılmaz Sarıaltın Betül Sever Yılmaz

https://doi.org/10.18016/ksutarimdoga.vi.1015574
Cited By: 1

Abstract

Helianthemum Türkiye’de 4’ü endemik olmak üzere 19 takson ile temsil edilmektedir. Bu çalışmada Türkiye’de doğal olarak yetişen Helianthemum oelandicum subsp. incanum toprak üstü kısımlarının etanollü (%75) ve sulu ekstrelerinin antioksidan, anti-inflamatuvar ve hipoglisemik aktiviteleri araştırılmıştır. Sulu ekstrenin DPPH ve ABTS radikalleri üzerine gösterdiği süpürücü etki miktarı sırasıyla 67.26±0.49 ve 68.08±0.41 µg ml-1 olarak tespit edilmiştir. Bitkinin etanollü ekstresinin DPPH radikaline karşı gösterdiği antioksidan aktivite değeri 46.71±0.27 µg ml-1 iken, ABTS radikali için bu değer 51.60±0.76 µg ml-1 olarak bulunmuştur. Bitkinin hem etanollü hem de sulu ekstresinin eritrosit membran hemolizini konsantrasyona bağlı olarak inhibe ederek in vitro anti-inflamatuvar etki gösterdiği ve etanollü ekstrenin sulu ekstreye göre daha güçlü etkiye sahip olduğu belirlenmiştir. Bitki ekstrelerinin in vitro hipoglisemik aktivite tayini için α-glukozidaza karşı inhibisyon etkileri ölçülmüş, etanollü ve sulu ekstrelerin IC50 değerleri sırasıyla 2.52±0.01 ve 3.21±0.01 µg ml-1 bulunmuş ve standart bileşik akarboz ile karşılaştırıldığında (IC50= 0.90±0.01 µg ml-1) α-glukozidaza karşı güçlü inhibisyon gösterdikleri tespit edilmiştir. Tüm aktivite sonuçları bir arada değerlendirildiğinde etanollü ekstrenin antioksidan, anti-inflamatuvar ve hipoglisemik aktivitesinin sulu ekstreden daha yüksek olduğu bulunmuştur.

Keywords

Serbest radikal süpürücü aktivite Anti-inflamatuvar aktivite Hipoglisemik aktivite Helianthemum oelandicum subsp. incanum.

References

  • Anokwuru, C.P., Anyasor, G.N., Ajibaye, O., Fakoya, O. & Okebugwu, P. (2011). Effect of extraction solvents on phenolic, flavonoid and antioxidant activities of three nigerian medicinal plants. Natural and Science, 9(7), 53-61.
  • Baldemir, A., Gokşen, N., Ildız, N., Şeker Karatoprak, G. & Koşar, M. (2017). Phytochemical profile and biological activities of Helianthemum canum L. BAUMG. from Turkey. Chemistry & Biodiversity, 14, 2-12.
  • Blois, M.S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 26, 1199-1200. https://doi.org/10.1002/cbdv.201700052
  • Barbosa, E., Calzada, F. & Campos, R (2006). Antigiardial Activity of Methanolic Extracts from Helianthemum glomeratum Lag. and Rubus coriifolius Focke in Suckling Mice CD-1. Journal of Ethnopharmacology 108, 395–397. https://doi.org/ 10.1016/j.jep.2006.05.026
  • Baytop, T. (1999). Türkiye’de Bitkilerle Tedavi (Therapy with Medicinal Plants in Turkey), Nobel Tıp Basımevi, İstanbul.
  • Benabdelaziz, I., Marcourt, L., Benkhaled, M., Wolfender, J.L. & Haba, H (2017). Antioxidant and antibacterial activities and polyphenolic constituents of Helianthemum sessiliflorum Pers. Natural Product Research, 31(6), 686-690. https://doi.org/10.1080/14786419.2016.1209669
  • Calzada, F. & Alanis, A.D. (2007). Additional antiprotozoal flavonol glycosides of the aerial parts of Helianthemum glomeratum. Phytotherapy Research, 21, 78-80. doi: 10.1002/ptr.2031
  • Chemam, Y., Benayache, S., Marchioni, E., Zhao, M., Mosset, P. & Benayache, F (2017). On-Line screening, isolation and identification of antioxidant compounds of Helianthemum ruficomum. Molecules, 22(239), 1-14. https://doi. org/10.3390/molecules22020239
  • Davis, P.H. (1965). Flora of Turkey and East Aegean Islands, Vol I, Edinburgh University Press, Edinburgh, UK.
  • Demir, T., Akpınar, Ö., Kara, H. & Güngör, H. (2019). Nar (Punica granatum L.) kabuğunun in vitro antidiyabetik, antienflamatuar, sitotoksik, antioksidan ve antimikrobiyal aktivitesi. Akademik Gıda, 17(1), 61-71. https://doi.org/ 10.24323/akademik-gida.544647
  • Demir, T., Akpınar, Ö., Kara, H. & Güngör, H. (2020). Cherry stem phenolic compounds: Optimization of extraction conditions and in vitro evaluations of antioxidant, antimicrobial, antidiabetic, anti-inflammatory, and cytotoxic activities. Journal of Food Processing and Preservation, 44, e14804. https://doi.org/10.1111/jfpp.14804
  • Djemam, N., Lassed, S., Gül, F., Altun, M., Monteiro, M., Menezes-Pinto, D., Benayache, S., Benayache, F., Zama, D., Demirtas, I. & Morato, M. (2020). Characterization of Ethyl Acetate and n-Butanol Extracts of Cymbopogon schoenanthus and Helianthemum lippii and their effect on the smooth muscle of the rat distal colon. Journal of Ethnopharmacology, 252, 112613. https://doi.org/ 10.1016/j.jep.2020.112613
  • Gunathilake, K.D.P.P., Ranaweera, K.K.D.S. & Rupasinghe, H.P. (2018). In vitro anti-inflammatory properties of selected green leafy vegetables. Biomedicines, 6(4), 107. https://doi.org/ 10.3390/ biomedicines6040107
  • Hamza, N., Berke, B., Umar, A., Cheze, C., Gin, H. & Moore, N. (2019). A review of algerian medicinal plants used in the treatment of diabetes. Journal of Ethnopharmacology, 238, 1-28. https://doi.org/ 10.1016/j.jep.2019.111841
  • Liu, X., Zhu, L., Tan, J., Zhou, X., Xiao, L., Yang, X. & Wang, B. (2014). Glucosidase inhibitory activity and antioxidant activity of flavonoid compound and triterpenoid compound from Agrimonia pilosa Ledeb. BMC Complementary and Alternative Medicine, 14(12), 1-10. https://doi.org/10.1186/ 1472-6882-14-12
  • Okur, M.E., Polat, D.C., Ozbek, H., Yilmaz, S., Yoltas, A. & Arslan, R. (2018). Evaluation of the antidiabetic property of capparis ovata desf. var. palaestina zoh. extracts using in vivo and in vitro approaches. Endocrine, Metabolic & Immune Disorders-Drug Targets (Formerly Current Drug Targets-Immune, Endocrine & Metabolic Disorders), 18(5), 489-501. https://doi.org/10.2174/ 1871530318666180328110524.
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237. https://doi.org/ 10.1016/s0891-5849(98)00315-3
  • Rubio-Moraga, A., Argandoña, J., Mota, B., Pérez, J., Verde, A., Fajardo, J., Gómez-Navarro, J., Castillo-López, R., Ahrazem, O. & Gómez-Gómez, L. (2013). Screening for polyphenols, antioxidant and antimicrobial activities of extracts from eleven Helianthemum taxa (Cistaceae) used in folk medicine in South-Eastern Spain. Journal of Ethnopharmacology, 148, 287-296. https://doi.org/ 10.1016/j.jep.2013.04.028
  • Shinde, U., Phadke, A., Nair, A., Mungantiwar, A., Dikshit, V. & Saraf, M. (1999). Membrane stabilizing activity—a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia, 70(3), 251-257. https://doi.org/10.1016/S0367-326X(99)00030-1
  • Sultana, B., Anwar, F. & Ashraf, M. (2009). Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules, 14, 2167-2180. https://doi.org/10.3390/ molecules14062167
  • Şam Gökşen, N. & Baldemir, A. (2016). Traditional usages of Helianthemum Mıller species. Journal of Health Sciences, 25, 49-52.
  • Terfassi, S., Dauvergne, X., Stephane Cerantola, S., Lemoine, C., Bensouici, C., Fadila, B., Christian M, Marchioni, E. & Benayache, S. (2021). First report on phytochemical investigation, antioxidant and antidiabetic activities of Helianthemum getulum. Natural Product Research, 1-8. https://doi.org/10.1080/14786419.2021.1928664
  • Yalcin, C.Ö., Yilmaz Sarialtin, S. & Cicek Polat, D. (2020). Quantification of phenolic and flavonoid contents and some biological activities of Ornithogalum sigmoideum Freyn & Sint. Journal of Research in Pharmacy, 24(4), 487-496. https://doi.org/10.35333/jrp.2020.197
There are 23 citations in total.

Details

Primary Language Turkish
Journal Section RESEARCH ARTICLE
Authors

Asli Can Agca 0000-0002-5710-3479

Sezen Yılmaz Sarıaltın 0000-0002-8387-4146

Betül Sever Yılmaz

Publication Date April 30, 2023
Submission Date October 27, 2021
Acceptance Date March 3, 2022
Published in Issue Year 2023Volume: 26 Issue: 2

Cite

APA Can Agca, A., Yılmaz Sarıaltın, S., & Sever Yılmaz, B. (2023). Türkiye’de Yetişen Helianthemum oelandicum subsp. incanum (Willk.) G. Lopez Bitkisinin Serbest Radikal Süpürücü, Anti-inflamatuvar ve Hipoglisemik Aktivitelerinin Değerlendirilmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(2), 227-233. https://doi.org/10.18016/ksutarimdoga.vi.1015574

Cited By

The Wound‐Healing Potential of the Endemic Plant Helianthemum canum (L.) Baumg: Preclinical Studies Supported with Phytochemical Profiling
Chemistry & Biodiversity
https://doi.org/10.1002/cbdv.202301529
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