Abstract
Bu çalışmada; 12 aspir genotipinin artan dozlarda BAP (0, 0.5, 1 ve 2 mg L-1) ve NAA (0, 0.1 ve 0.5 mg L-1) içeren MS ortamında anter kültürüne karşı tepkileri belirlenmiştir. Donör bitkiler tarla koşullarında yetiştirilmiş ve ana çiçek tablaları erken veya geç tek çekirdekli dönemde hasat edilmiştir. Ön işlem olarak +4 °C’de ve 3-4 gün süreyle soğuk muamelesi uygulanmıştır. Aspir genotiplerinden sadece Balcı ve Linas çeşitleri rejenere olabilir kallus üretmiştir. Balcı çeşidi daha yüksek oranda kallus oluşturmasına rağmen sadece Linas çeşidinde 0.5 veya 1 mg L-1 BAP + 0.1 mg L-1 NAA kombinasyonlarından sürgün rejenerasyonu gerçekleşmiştir. Toplamda Linas çeşidine ait 8 sürgün 0.1 mg L-1 NAA içeren ½ MS ortamına aktarılmış, ancak köklenme sağlanamamıştır. Sonuç olarak; 12 aspir genotipi arasında Balcı ve Linas çeşitlerinin anter kültürü rejenerasyon potansiyelinin daha yüksek olduğu belirlenmiştir. Bununla birlikte, bu çeşitler üzerinde gelecekte sürgün geliştirme ve köklendirme çalışmalarına odaklanılması gerekmektedir.
Keywords
Project Number
202023029
References
- Belide, S,, Hac, L., Singh, S.P., Green, A.G., & Wood, C.C. (2011). Agrobacterium-mediated transformation of safflower and the efficient recovery of transgenic plants via grafting. Plant Methods, 7(1), 12. https://doi.org/10.1186/1746-4811-7-12.
- Khandakar, R.K., Jie, Y.U., Sun-Kyung, M.I.N., Mi-Kyoung, W.O.N., Choi, H.G., Park, H.S., Choi, J.J., Chae, S.C., Jung, J.Y., Lee, K.M., Kim, T.S., & Park, Y.J. (2014). Regeneration of haploid plantlet through anther culture of Chrysanthemum (Dendranthema grandiflorum). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 42(2), 482-487. https://doi.org/10.15835/nbha4229640.
- Kumar, K.R., Singh, K.P., Jain, P.K., Raju, D.V.S., Kumar, P., Reeta, B., & Panwar, S. (2018). Influence of growth regulators on callus induction and plant regeneration from anthers of Tagetes spp. Indian Journal of Agricultural Sciences, 88(6), 970-977. Retrieved from https://www.researchgate.net/ publication/325780165_Influence_of_growth_regulators_on_callus_induction_and_plant_regeneration_from_anthers_of_Tagetes_spp.
- Lantos, C., Lehoczki-Krsjak, S., & Pauk, J. (2022). Induction of in vitro androgenesis in anther culture of recalcitrant einkorn (Triticum monococcum L.). Plant Cell Tissue Organ Culture, 50, 417-426. https://doi.org/10.1007/s11240-022-02293-6.
- Liu, L., Guan, L.L., & Yang, Y.X. (2016). A review of fatty acids and genetic characterization of safflower (Carthamus tinctorius L.) seed oil. Organic Chemistry: Current Research, 5, 160-163. Doi: 10.15806/j.issn.2311-8571.2016.0006.
- Matthaus, B., Özcan, M.M., & Al Juhaimi, F.Y. (2015). Fatty acid composition and tocopherol profiles of safflower (Carthamus tinctorius L.) seed oils. Natural product research, 29(2), 193-196. https://doi.org/10.1080/14786419.2014.971316.
- Menegaes, J.F., & Nunes, U.R. (2020). Safflower: importance, use and economical exploitation. Scientia Agraria Paranaensis, 1(1), 1-11. https:// doi.org/10.18188/sap.v19i1.21250.
- Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x.
- Pandey, A.K., & Kumari, A. (2008). Pollination ecology of safflower (Carthamus tinctorius linn)", paper presented at: Safflower: unexploited potential and world adaptability, 7 th International Safflower Conference, Wagga Wagga, NSW, Australia, 3-6 November, pg, 1-10. Retrieved from https://www. cabdirect.org/cabdirect/FullTextPDF/2009/20093258236.pdf.
- Prasad, B.R., Khadeer, M.A., Seeta, P., & Anwar, S.Y. (1990). Influence of genotype and cold pre-treatment on anther culture response in safflower, Carthamus tinctorius L. Indian Journal of Experimental Biology, 28 (10), 924-927. Retrieved from https://www.cabdirect.org/cabdirect/abstract/ 19921633108.
- Prasad, B.R., Khadeer, M.A., Seeta, P., & Anwar, S.Y. (1991). In vitro induction of androgenic haploids in safflower (Carthamus tinctorius L.). Plant cell reports, 10(1), 48-51. https://doi.org/10.1007/ BF00233032.
- Rahamatalla, A.B., Babiker, E.E., Krishna, A.G., El Tinay, A.H. (2001). Changes in fatty acids composition during seed growth and physicochemical characteristics of oil extracted from four safflower cultivars. Plant Foods for Human Nutrition, 56, 385-395. https://doi.org/ 10.1023/A:1011860810082.
- Thengane, S.R., Joshi, M.S., Khuspe, S.S., & Mascarenhas, A.F. (1994). Anther culture in Helianthus annuus L., influence of genotype and culture conditions on embryo induction and plant regeneration. Plant Cell Reports, 13(3), 222-226. https://doi.org/10.1007/BF00239897.
- Voronova, O. (2016). Callus formation and plant regeneration in sunflower (Helianthus L., Asteraceae in vitro tissue culture, 19th International Sunflower Conference, Edirne, p.211. Retrieved from https://www.isasunflower.org/ fileadmin/documents/19thISCEDIRNE2016/Physiology/Voronova.pdf..
Abstract
In this study; the responses of 12 safflower genotypes to anther culture were determined in MS medium containing increasing doses of BAP (0, 0.5, 1, and 2 mg L-1) and NAA (0, 0.1, and 0.5 mg L-1). Donor plants were grown under field conditions and the main capitulas were collected in early or late uninucleate stage. A pre-treatment was applied to their capitules at +4 °C for 3-4 days. Only cvs. Balcı and Linas produced regenerable callus among these genotypes. Although regenerable callus rate was higher in Balcı than Linas, shoot regeneration was achieved only in Linas with 0.5 or 1 mg L-1 of BAP + 0.1 mg L-1 of NAA combinations. In total, 8 shoots of Linas were moved to ½ MS medium consisting of 0.1 mg L-1 of NAA, but rooting of them could not be performed. In sum, among the 12 safflower genotypes, cvs. Balcı and Linas have higher anther culture regeneration potential; however, it is necessary to focus on shoot development and rooting on these cultivars in further experiments.
Keywords
Supporting Institution
Eskişehir Osmangazi University Scientific Research Projects Commission
Project Number
202023029
Thanks
This study was provided by Eskişehir Osmangazi University Scientific Research Projects Commission (Project code: 202023029). In addition, this study was prepared by using of the master thesis of Dudu Büşra Duru.
References
- Belide, S,, Hac, L., Singh, S.P., Green, A.G., & Wood, C.C. (2011). Agrobacterium-mediated transformation of safflower and the efficient recovery of transgenic plants via grafting. Plant Methods, 7(1), 12. https://doi.org/10.1186/1746-4811-7-12.
- Khandakar, R.K., Jie, Y.U., Sun-Kyung, M.I.N., Mi-Kyoung, W.O.N., Choi, H.G., Park, H.S., Choi, J.J., Chae, S.C., Jung, J.Y., Lee, K.M., Kim, T.S., & Park, Y.J. (2014). Regeneration of haploid plantlet through anther culture of Chrysanthemum (Dendranthema grandiflorum). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 42(2), 482-487. https://doi.org/10.15835/nbha4229640.
- Kumar, K.R., Singh, K.P., Jain, P.K., Raju, D.V.S., Kumar, P., Reeta, B., & Panwar, S. (2018). Influence of growth regulators on callus induction and plant regeneration from anthers of Tagetes spp. Indian Journal of Agricultural Sciences, 88(6), 970-977. Retrieved from https://www.researchgate.net/ publication/325780165_Influence_of_growth_regulators_on_callus_induction_and_plant_regeneration_from_anthers_of_Tagetes_spp.
- Lantos, C., Lehoczki-Krsjak, S., & Pauk, J. (2022). Induction of in vitro androgenesis in anther culture of recalcitrant einkorn (Triticum monococcum L.). Plant Cell Tissue Organ Culture, 50, 417-426. https://doi.org/10.1007/s11240-022-02293-6.
- Liu, L., Guan, L.L., & Yang, Y.X. (2016). A review of fatty acids and genetic characterization of safflower (Carthamus tinctorius L.) seed oil. Organic Chemistry: Current Research, 5, 160-163. Doi: 10.15806/j.issn.2311-8571.2016.0006.
- Matthaus, B., Özcan, M.M., & Al Juhaimi, F.Y. (2015). Fatty acid composition and tocopherol profiles of safflower (Carthamus tinctorius L.) seed oils. Natural product research, 29(2), 193-196. https://doi.org/10.1080/14786419.2014.971316.
- Menegaes, J.F., & Nunes, U.R. (2020). Safflower: importance, use and economical exploitation. Scientia Agraria Paranaensis, 1(1), 1-11. https:// doi.org/10.18188/sap.v19i1.21250.
- Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x.
- Pandey, A.K., & Kumari, A. (2008). Pollination ecology of safflower (Carthamus tinctorius linn)", paper presented at: Safflower: unexploited potential and world adaptability, 7 th International Safflower Conference, Wagga Wagga, NSW, Australia, 3-6 November, pg, 1-10. Retrieved from https://www. cabdirect.org/cabdirect/FullTextPDF/2009/20093258236.pdf.
- Prasad, B.R., Khadeer, M.A., Seeta, P., & Anwar, S.Y. (1990). Influence of genotype and cold pre-treatment on anther culture response in safflower, Carthamus tinctorius L. Indian Journal of Experimental Biology, 28 (10), 924-927. Retrieved from https://www.cabdirect.org/cabdirect/abstract/ 19921633108.
- Prasad, B.R., Khadeer, M.A., Seeta, P., & Anwar, S.Y. (1991). In vitro induction of androgenic haploids in safflower (Carthamus tinctorius L.). Plant cell reports, 10(1), 48-51. https://doi.org/10.1007/ BF00233032.
- Rahamatalla, A.B., Babiker, E.E., Krishna, A.G., El Tinay, A.H. (2001). Changes in fatty acids composition during seed growth and physicochemical characteristics of oil extracted from four safflower cultivars. Plant Foods for Human Nutrition, 56, 385-395. https://doi.org/ 10.1023/A:1011860810082.
- Thengane, S.R., Joshi, M.S., Khuspe, S.S., & Mascarenhas, A.F. (1994). Anther culture in Helianthus annuus L., influence of genotype and culture conditions on embryo induction and plant regeneration. Plant Cell Reports, 13(3), 222-226. https://doi.org/10.1007/BF00239897.
- Voronova, O. (2016). Callus formation and plant regeneration in sunflower (Helianthus L., Asteraceae in vitro tissue culture, 19th International Sunflower Conference, Edirne, p.211. Retrieved from https://www.isasunflower.org/ fileadmin/documents/19thISCEDIRNE2016/Physiology/Voronova.pdf..
Details
| Primary Language | English |
|---|---|
| Subjects | Agricultural, Veterinary and Food Sciences |
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Project Number | 202023029 |
| Publication Date | April 30, 2023 |
| Submission Date | March 7, 2022 |
| Acceptance Date | August 24, 2022 |
| Published in Issue | Year 2023Volume: 26 Issue: 2 |
Cited By
Morphological, Physiological and Biochemical Responses of Safflower (Carthamus tinctorius L.) Exposed to Salinity Stress
Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi
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