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Mentha pulegium extracts showed strong antimicrobial and cytotoxic effects in vitro.

Year 2023, Volume: 26 Issue: 6, 1291 - 1304, 31.12.2023

Semih Dalkılıç Lütfiye Kadıoğlu Dalkılıç Songül Fidan Mustafa Timurkaan Lütfü Uygur Gökhan Akay İsmail Korkmaz

https://doi.org/10.18016/ksutarimdoga.vi.1192081
Cited By: 1

Abstract

Mentha pulegium is a medicinally important and well-known plant and used for various purposes such as medicinal, nutritional and spice. We are analyzed to observe the antimicrobial, antioxidant and cytotoxic properties of M. pulegium extracts in this study. The antimicrobial activity of M. pulegium was tested using the agar well method. MIC, MBC and antimicrobial activity were tested on Staphylococcus aureus (ATCC 25923), Klebsiella pneumoniae (ATCC 700603), Escherichia coli (ATCC 25322), Bacillus megaterium (DSM32) and Candida albicans (FMC17) microorganisms. Clindamycin (2 µg) and Amoxicillin (30 µg) were used as positive control and Dimethylsulfoxide (DMSO) as negative control. Cytotoxic activity of extracts at different concentrations obtained from solvents such as acetone, chloroform and methanol using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test method; µg/ml cytotoxic activity and cell death patterns after exposure to different concentrations of the extracts on human breast cancer (MDA-MB-231), human pancreatic cancer (PANC1), human ovarian cancer (OVCAR3) and human lung cancer (A549) cell lines determined accordingly. As a result, it was determined that M. pulegium extract has a strong antimicrobial activity spectrum and cytotoxic effect.

Keywords

Antimicrobial Antioxidant Apoptosis Cytotoxicity Mentha pulegium

Supporting Institution

TÜBİTAK

Project Number

1919B012001186

Thanks

Thanks to Prof. Dr. Mustafa Kaplan, Fırat University Faculty of Medicine Department of Parasitology, for contributing to the laboratory environment in the realization of this study.

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Mentha pulegium ekstraktı güçlü in vitro antimikrobiyal ve sitotoksik etki göstermiştir

Year 2023, Volume: 26 Issue: 6, 1291 - 1304, 31.12.2023

Semih Dalkılıç Lütfiye Kadıoğlu Dalkılıç Songül Fidan Mustafa Timurkaan Lütfü Uygur Gökhan Akay İsmail Korkmaz

https://doi.org/10.18016/ksutarimdoga.vi.1192081
Cited By: 1

Abstract

Mentha pulegium tıbbi açıdan önemli ve iyi bilinen bir bitkidir ve tıbbi, beslenme ve baharat gibi çeşitli amaçlar için kullanılır. Bu çalışmada M. pulegium ekstraktının antimikrobiyal, antioksidan ve sitotoksik özelliklerini görmek için analiz edildi. M. pulegium'un antimikrobiyal aktivitesi agar kuyucuk yöntemi kullanılarak test edildi. MIC, MBC ve antimikrobiyal aktivite Staphylococcus aureus (ATCC 25923), Klebsiella pneumoniae (ATCC 700603), Escherichia coli (ATCC 25322), Bacillus megaterium (DSM32) ve Candida albicans (FMC17) mikroorganizmaları üzerinde test edildi. Pozitif kontrol olarak Klindamisin (2 µg) ve Amoksisilin (30 µg), negatif kontrol olarak Dimetilsülfoksit (DMSO) kullanıldı. 3-(4,5-dimetiltiyazol-2-il) -2,5-difenil tetrazolyum bromür (MTT) test yöntemi kullanılarak aseton, kloroform ve metanol gibi çözücülerden elde edilen farklı konsantrasyonlarda ekstraktların sitotoksik aktivitesi; ekstraktların farklı konsantrasyonlarına maruz kaldıktan sonra µg/ml sitotoksik aktivite ve hücre ölüm şekilleri; Aseton, kloroform ve metanol gibi çözücülerden 3-(4,5-dimetiltiyazol-2-il) -2,5-difenil tetrazolyum bromür (MTT) test yöntemi kullanılarak aseton, kloroform ve metanol insan meme kanseri (MDA-MB-231), insan pankreas kanseri (PANC1), insan yumurtalık kanseri (OVCAR3) ve insan akciğer kanseri (A549) hücre hatları buna göre belirlendi. Sonuç olarak M. pulegium ekstraktının güçlü bir antimikrobiyal aktivite spektrumuna ve sitotoksik etkiye sahip olduğu belirlenmiştir.

Keywords

Antimikrobiyal Antioksidan Apoptoz Sitotoksik Mentha pulegium

Project Number

1919B012001186

References

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  • 18. Ebana RUB, Etim OE, Edet UO, John RS, Osom AO. Comparative evaluation of phytochemicals and antibacterial activity of the roots and leaves of anthocleista vogelii on some clinical ısolates. Asian Journal of Research in Botany. 2018;1(1):1-8. https://journalajrib.com/index.php/AJRIB/article/view/1118
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There are 50 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section RESEARCH ARTICLE
Authors

Semih Dalkılıç 0000-0002-6892-247X

Lütfiye Kadıoğlu Dalkılıç 0000-0002-6791-3811

Songül Fidan 0000-0003-1876-858X

Mustafa Timurkaan 0000-0003-1950-0489

Lütfü Uygur 0000-0002-5113-2703

Gökhan Akay 0000-0003-3892-2572

İsmail Korkmaz 0000-0003-4631-7786

Project Number 1919B012001186
Early Pub Date June 14, 2023
Publication Date December 31, 2023
Submission Date October 20, 2022
Acceptance Date February 27, 2023
Published in Issue Year 2023Volume: 26 Issue: 6

Cite

APA Dalkılıç, S., Kadıoğlu Dalkılıç, L., Fidan, S., Timurkaan, M., et al. (2023). Mentha pulegium extracts showed strong antimicrobial and cytotoxic effects in vitro. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(6), 1291-1304. https://doi.org/10.18016/ksutarimdoga.vi.1192081

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