Yıl 2018, Cilt 21, Sayı 5, Sayfalar 672 - 677 2018-10-31

Multiple shoot regeneration of an important equatorial forage plant Indigofera zollingeriana Miq.
Önemli bir ekvator yem bitkisi olan Indigofera zollingerian Miq.’nın çoklu sürgün rejenerasyonu

Çiğdem Alev ÖZEL [1] , Siti MAESAROH [2]

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Indigofera zollingeriana is an important protein rich forage   and dye plant species that grows widely in Indonesia. It is difficult to propagate the plant through seeds due to high dormancy. Therefore, there is need to develop both in vitro and ex vitro propagation techniques of I. zollingeriana. This study aimed to develop a protocol for multiple shoot regeneration of the plant under in vitro conditions using different combinations of   BAP+0.01 mg/L NAA. The results showed that both epigeal and cotyledon node explants have high potential to regenerate with maximum number of regenerated shoots noted on the epigeal and the cotyledon node explants using MS medium containing 1.5 mg/L BAP + 0.01 mg/L of NAA and 1.0 mg/L BAP + 0.01 mg/L of NAA in the same order. The shoots regenerated on all combinations of BAP+NAA were rooted using 0.5 mg/L IBA that promoted rooting in effective manner. The rooted plants were easily acclimatized to external conditions in an environmental chamber. The results suggest that I. zollengriana plant species is no more difficult to regenerate under in vitro conditions. This research will aid in future studies related to breeding, propagation, biosynthesis and extraction of active compounds.

Indigofera zollingeriana, Endonezya'da yaygın olarak yetişen proteince zengin yem ve boya bitkisidir. Bitkiyi tohumdan çoğaltmak yüksek dormansi  sebebiyle zor olmaktadır. Bu nedenle, I. zollingeriana'nın in vitro ve ex vitro koşulllarda çoğaltma tekniklerinin geliştirilmesi gerekmektedir. Bu çalışmada, farklı kombinasyonlarda BAP +0.01 mg/L NAA içeren MS ortamı kullanılarak  in vitro koşullar altında bitkinin hızlı çoğaltım için bir protokol geliştirmesi amaçlanmıştır. En fazla sürgün rejenerasyonu epigeal ve kotiledon nodlarının sırasıyla 1.5 mg / L BAP + 0.01 mg / L NAA ve 1.0 mg/L BAP + 0.01 mg/L NAA içeren MS ortamında elde edilmiştir. BAP + NAA'nın tüm kombinasyonlarında rejenerasyonu yapılan sürgünler 0.5 mg/L IBA kullanılarak başarılı şekilde köklendirilmiştir. Köklü bitkiler, bir iklim dolabında dış koşullara alıştırılmıştır. Bu çalışma I. zollingeriana bitkisinin in vitro koşullar altında yetiştirilebileceğini göstermiş olması ve elde edilen sonuçların ilerideki çalışmalarda aktif bileşiklerin biyosentezi ve ekstraksiyonu ile bitki yetiştirilmesi ve çoğaltılmasına yardımcı olacağını düşünülmektedir.
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Orcid: 0000-0000-0000-0000
Yazar: Çiğdem Alev ÖZEL (Sorumlu Yazar)

Orcid: 0000-0000-0000-0000
Yazar: Siti MAESAROH

Bibtex @araştırma makalesi { ksutarimdoga442431, journal = {Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi}, issn = {}, eissn = {2619-9149}, address = {Kahramanmaraş Sütçü İmam Üniversitesi}, year = {2018}, volume = {21}, pages = {672 - 677}, doi = {10.18016/ksudobil.386167}, title = {Multiple shoot regeneration of an important equatorial forage plant Indigofera zollingeriana Miq.}, key = {cite}, author = {ÖZEL, Çiğdem Alev and MAESAROH, Siti} }
APA ÖZEL, Ç , MAESAROH, S . (2018). Multiple shoot regeneration of an important equatorial forage plant Indigofera zollingeriana Miq.. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 21 (5), 672-677. DOI: 10.18016/ksudobil.386167
MLA ÖZEL, Ç , MAESAROH, S . "Multiple shoot regeneration of an important equatorial forage plant Indigofera zollingeriana Miq.". Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 21 (2018): 672-677 <http://dogadergi.ksu.edu.tr/issue/38256/442431>
Chicago ÖZEL, Ç , MAESAROH, S . "Multiple shoot regeneration of an important equatorial forage plant Indigofera zollingeriana Miq.". Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 21 (2018): 672-677
RIS TY - JOUR T1 - Multiple shoot regeneration of an important equatorial forage plant Indigofera zollingeriana Miq. AU - Çiğdem Alev ÖZEL , Siti MAESAROH Y1 - 2018 PY - 2018 N1 - doi: 10.18016/ksudobil.386167 DO - 10.18016/ksudobil.386167 T2 - Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi JF - Journal JO - JOR SP - 672 EP - 677 VL - 21 IS - 5 SN - -2619-9149 M3 - doi: 10.18016/ksudobil.386167 UR - http://dx.doi.org/10.18016/ksudobil.386167 Y2 - 2018 ER -
EndNote %0 Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi Multiple shoot regeneration of an important equatorial forage plant Indigofera zollingeriana Miq. %A Çiğdem Alev ÖZEL , Siti MAESAROH %T Multiple shoot regeneration of an important equatorial forage plant Indigofera zollingeriana Miq. %D 2018 %J Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi %P -2619-9149 %V 21 %N 5 %R doi: 10.18016/ksudobil.386167 %U 10.18016/ksudobil.386167
ISNAD ÖZEL, Çiğdem Alev , MAESAROH, Siti . "Önemli bir ekvator yem bitkisi olan Indigofera zollingerian Miq.’nın çoklu sürgün rejenerasyonu". Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 21 / 5 (Ekim 2018): 672-677. http://dx.doi.org/10.18016/ksudobil.386167