Yıl 2018, Cilt 21, Sayı 6, Sayfalar 950 - 956 2018-11-30

Cytotoxic Effect of Rhododendron luteum Leaf Extract on Human Cancer Cell Lines
Rhododendron luteum Yaprak Ekstraktının İnsan Kanser Hücre Serilerindeki Sitotoksik Etkisi

Selim DEMİR [1] , Ibrahim TURAN [2] , Yuksel ALIYAZICIOGLU [3]

45 99

Rhododendron luteum belongs to the genus Rhododendron, members of which are frequently used in folk medicine. Various studies have investigated the cytotoxic effect of different Rhododendron species, but there have been limited studies of the cytotoxic effect of R. luteum. The aim of this study was to investigate the antioxidant properties and cytotoxic effect of R. luteum leaf extract on the human cancer cells. The total phenolic content (TPC), total flavonoid content (TFC), and reducing power of the extract were evaluated using spectrophotometric procedures. The cytotoxic effect of the extract on five cancers (human breast, colon, lung, prostate, and liver carcinoma) and human fibroblast cells was determined using the MTT assay. TPC and reducing power values of extract were found 173.2±3.97 mg gallic acid equivalents, and 629.5±3.49 mg trolox equivalents per to g sample, respectively. R. luteum leaf extract exhibited selective cytotoxicity especially against colon (1.9 fold) and liver (2.2 fold) cancer cells compared to normal fibroblast cells. This is the first study to reveal the antiproliferative effect of R. luteum leaf extract. Further studies are now needed to identify the cytotoxic molecules in the extract and their mechanisms.

Rhododendron luteum, Rhododendron cinsine dahil bir bitki olup, bu cinse ait bitkiler geleneksel tedavide sıklıkla kullanılmaktadır. Rhododendron türlerinin sitotoksik etkilerini araştıran çok sayıda çalışma olmasına rağmen, R. luteum türünün sitotoksik etkilerini inceleyen çalışmalar sınırlıdır. Bu çalışmanın amacı R. luteum yaprak ekstraktının antioksidan özelliklerini ve insan kanser hücreleri üzerindeki sitotoksik etkisini araştırmaktır. Ekstraktın toplam fenolik içeriği (TPC), toplam flavonoid içeriği (TFC) ve indirgeyici gücü spektrofotometrik prosedürler kullanılarak değerlendirildi. Ekstraktın beş kanser (insan meme, kolon, akciğer, prostat ve karaciğer karsinomu) ve insan fibroblast hücreleri üzerindeki sitotoksik etkisi MTT analizi kullanılarak belirlendi. Ekstraktın TPC ve indirgeyici güç değerleri g örnek başına sırasıyla 173.2±3.97 mg gallik asit eşdeğeri ve 629.5±3.49 mg troloks eşdeğeri olarak bulundu. R. luteum yaprak ekstraktı normal fibroblast hücrelerine kıyasla özellikle kolon (1.9 kat) ve karaciğer (2.2 kat) kanser hücrelerine karşı seçici sitotoksisite sergiledi. Bu çalışma R. luteum yaprak ekstraktının antiproliferatif etkisini ortaya koyan ilk çalışmadır. Ekstraktta bulunan sitotoksik moleküllerin tanımlanması ve etki mekanizmalarının belirlenmesi için daha kapsamlı çalışmalara ihtiyaç vardır.

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Orcid: 0000-0002-1863-6280
Yazar: Selim DEMİR (Sorumlu Yazar)
Kurum: Karadeniz Technical Universty
Ülke: Turkey


Orcid: 0000-0003-3400-5494
Yazar: Ibrahim TURAN
Kurum: Gümüşhane Üniversitesi | Gümüşhane University

Orcid: 0000-0001-9474-4307
Yazar: Yuksel ALIYAZICIOGLU
Kurum: Karadeniz Technical Universty

Bibtex @araştırma makalesi { ksutarimdoga421283, journal = {Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi}, issn = {}, eissn = {2619-9149}, address = {Kahramanmaraş Sütçü İmam Üniversitesi}, year = {2018}, volume = {21}, pages = {950 - 956}, doi = {10.18016/ksutarimdoga.vi.421283}, title = {Cytotoxic Effect of Rhododendron luteum Leaf Extract on Human Cancer Cell Lines}, key = {cite}, author = {ALIYAZICIOGLU, Yuksel and DEMİR, Selim and TURAN, Ibrahim} }
APA DEMİR, S , TURAN, I , ALIYAZICIOGLU, Y . (2018). Cytotoxic Effect of Rhododendron luteum Leaf Extract on Human Cancer Cell Lines. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 21 (6), 950-956. DOI: 10.18016/ksutarimdoga.vi.421283
MLA DEMİR, S , TURAN, I , ALIYAZICIOGLU, Y . "Cytotoxic Effect of Rhododendron luteum Leaf Extract on Human Cancer Cell Lines". Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 21 (2018): 950-956 <http://dogadergi.ksu.edu.tr/issue/39291/421283>
Chicago DEMİR, S , TURAN, I , ALIYAZICIOGLU, Y . "Cytotoxic Effect of Rhododendron luteum Leaf Extract on Human Cancer Cell Lines". Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 21 (2018): 950-956
RIS TY - JOUR T1 - Cytotoxic Effect of Rhododendron luteum Leaf Extract on Human Cancer Cell Lines AU - Selim DEMİR , Ibrahim TURAN , Yuksel ALIYAZICIOGLU Y1 - 2018 PY - 2018 N1 - doi: 10.18016/ksutarimdoga.vi.421283 DO - 10.18016/ksutarimdoga.vi.421283 T2 - Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi JF - Journal JO - JOR SP - 950 EP - 956 VL - 21 IS - 6 SN - -2619-9149 M3 - doi: 10.18016/ksutarimdoga.vi.421283 UR - http://dx.doi.org/10.18016/ksutarimdoga.vi.421283 Y2 - 2018 ER -
EndNote %0 Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi Cytotoxic Effect of Rhododendron luteum Leaf Extract on Human Cancer Cell Lines %A Selim DEMİR , Ibrahim TURAN , Yuksel ALIYAZICIOGLU %T Cytotoxic Effect of Rhododendron luteum Leaf Extract on Human Cancer Cell Lines %D 2018 %J Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi %P -2619-9149 %V 21 %N 6 %R doi: 10.18016/ksutarimdoga.vi.421283 %U 10.18016/ksutarimdoga.vi.421283
ISNAD DEMİR, Selim , TURAN, Ibrahim , ALIYAZICIOGLU, Yuksel . "Rhododendron luteum Yaprak Ekstraktının İnsan Kanser Hücre Serilerindeki Sitotoksik Etkisi". Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 21 / 6 (Kasım 2018): 950-956. http://dx.doi.org/10.18016/ksutarimdoga.vi.421283