Year 2019, Volume 22, Issue 4, Pages 506 - 511 2019-08-31

The Combine Effects of Eicosanoid Biosynthesis Inhibitors and Different Isolates of Metarhizium anisopliae (Metschn.) and Beauveria bassiana (Balsamo) (Deuteromycota: Hyphomycetes) on the Mortality of Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae) Larvae
Metarhizium Anisopliae ve Beauveria Bassiana'nın Farklı İzolatlarının Eikosanoid Biyosentezi İnhibitörleri ile Birlikte Spodoptera Littoralis Larvalarına Uygulandığında Larvalar Üzerindeki Ölüm Etkisi

Hasan TUNAZ [1] , Mehmet Kubilay ER [2] , Ali Arda IŞIKBER [3]

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The effects of entomopathogen fungus isolates on the mortality of Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae) larvae were increased and accelerated when co-administered with eicosanoid inhibitors (EBIs) (Dexamethasone, Indomethacin, Esculetin Phenidone Ibuprofen and Naproxen). The mode of action of these compounds are different. When eicosanoid inhibitors were applied to S. littoralis larvae together with entomopathogenic fungus isolates (Beauveria 6646 and Metarhizium 3293), they accelerated deaths of the insect and consequently increased mortality rates. In addition, when different doses of Phenidone, one of the eicosanoid inhibitors, were administered to the larvae with the fungal isolates, there was a significant increase in mortality due to the dose of Phenidone. These results showed that the application of eicosanoid inhibitors with fungal agents to S. littoralis larvae increased the effectiveness of these potential microbial control agents.

(Boisduval) (Lepidoptera: Noctuidae) larvalarının ölümü üzerindeki etkileri eikosanoid inhibitörleri (EBI'ler) (Deksametazon, İndometasin, Esculetin Fenidon Ibuprofen ve Naproksen) ile birlikte larvalara uygulandığında, larvaların ölüm oranı artmış ve hızlanmıştır. Eikosanoid inhibitörlerinin etki mekanizması birbirlerinden farklıdır. Entomopatojen fungus izolatları (Beauveria 6646 ve Metarhizium 3293) ile eikosanoid inhibitörleri birlikte S. littoralis larvalarına uygulandığında, böceğin ölüm oranlarını önemli derecede artırdığı gibi aynı zamanda böcek ölümleri hızlanmıştır. Ek olarak, bahsedilen fungus izolatları ile larvalara farklı dozlarda eikosanoid inhibitörlerinden Phenidone uygulandığında, Phenidone dozuna bağlı olarak larva ölüm oranında önemli bir artış olmuştur. Bu sonuçlar, eikosanoid inhibitörlerinin fungal etmenlerle birlikte S. littoralis larvalarına uygulanmasının, potansiyel mikrobiyal kontrol etmenlerinin bu zararlı üzerinde etkinliğini arttırdığını göstermiştir.Entomopatojen fungus izolatlarının Spodoptera littoralis

  • Connick WJ, Osbring WLA, Wright MS, Williams KS, Daigle DJ, Boykin DL, Lax AR 2001. Increased mortality of Coptotermes formosanus (Isoptera: Rhinotermitidae) exposed to eicosanoid biosynthesis inhibitors and Serratia marcescens (Eubacteriales: Enterobactericeae). Environ Entomology 30:449-455.
  • Dean P, Gadsden JC, Richards EH, Edwards JP, Charnley AK, Reynolds SE 2002. Modulation by eicosanoid biosynthesis inhibitors of immune responses by the insect Manduca sexta to the pathogenic fungus Metarhizium anisopliae. Journal of Invertebrate Patholology 79: 93-101.
  • Gillespie JA, Kanaost MR, Trenzcek T 1997. Biological mediators of insect imunity. Annual Review Entomology 47:611-643.
  • Leulier F, Parquet C, Pili-Floury S, Ryu JH, Caroff M, Lee WJ, Mengin-Lecreulx D, Lemaitre B 2003. The Drosophila immune system detects bacteria through specific peptidoglycan recognition. Nature Immunology 4: 478–484.
  • Lord JJ, Anderson S, Stanley DW 2002. Eicosanoids mediate Manduca sexta cellular response to the fungal pathogen Beauveria bassiana: A role for the lipoxygenase pathway. Archives Insect Biochemistry Physiology 51: 46–54.
  • Mandato CA, Diehl-Jones WL, Moore SJ, Downer RGH 1997. The effects of eicosanoid biosynthesis inhibitors on prophenoloxidase activation, phagocytosis and cell spreading in Galleria mellonella. Journal of Insect Physiolology 43: 1-8.
  • Miller JS, Nguyen T, Stanley-Samuelson DW 1994. Eicosanoids mediate insect nodulation responses to bacterial infections. Proceedings of the National Academy of Sciences of the United States of America 91: 12418-12422.
  • Morishima I, Yamano Y, Inoue K, Matsuo N 1997. Eicosanoids mediate induction of immune genes in the fat body of the silkworm, Bombyx mori. FEBS Letters 419: 83-86.
  • SAS Institute Inc. 1989. SAS/STATR User's Guide, Version 6, 4th Ed., vol 2. SAS Institute Inc., Cary, NC.
  • Stanley-Samuelson DW, Jensen E, Nickerson KW, Tiebel K, Ogg CL, Howard RW 1991. Insect immune response to bacterial infection is mediated by eicosanoids. Proceedings of the National Academy of Sciences of the United States of America 88: 1064-1068.
  • Stanley DW 2006. Prostaglandins and other eicosanoids in insects: biological significance. Annual Review Entomology 51:25-44.
  • Stanley DW, Miller JS 2006. Eicosanoid actions in insect cellular immune functions. Entomologia Experimentalis et Applicata 119: 1-13.
  • Tunaz H 2006. Eicosanoid Biosynthesis Inhibitors Influence Mortality of Pieris brassicae Larvae Co-Injected With Fungal Conidia. Archives Insect Biochemistry Physiology 63:93-100.
  • Tunaz H, Küsek M 2012. The Role of Eicosanoid Biosynthesis Inhibitors on mortality of Blattella germanica adults Co-Injected with the bacteria; Serratia marcescens Second International Symposium of Biopesticides and Ecotoxicological Network. September 24-26, 2012 Bangkok, Thailand, Pp 29-32.
  • Tunaz H, Küsek M 2015. Mortality effects of eicosanoid biosynthesis inhibitors on Spodoptera littoralis larvae co-injected with the bacteria, Serratia marcescens. Turkish Journal of Entomology 39:121-127.
Primary Language en
Subjects Science
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0003-4942-6056
Author: Hasan TUNAZ (Primary Author)
Institution: KAHRAMANMARAŞ SÜTÇÜ İMAM ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ, BİTKİ KORUMA BÖLÜMÜ

Orcid: 0000-0003-1568-8656
Author: Mehmet Kubilay ER

Orcid: 0000-0003-1236-4648
Author: Ali Arda IŞIKBER

Dates

Publication Date: August 31, 2019

APA TUNAZ, H , ER, M , IŞIKBER, A . (2019). The Combine Effects of Eicosanoid Biosynthesis Inhibitors and Different Isolates of Metarhizium anisopliae (Metschn.) and Beauveria bassiana (Balsamo) (Deuteromycota: Hyphomycetes) on the Mortality of Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae) Larvae. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 22 (4), 506-511. DOI: 10.18016/ksutarimdoga.vi.525317