The Role of Eicosanoids in Nodulation Reaction Against Entomopathogenic Fungi in Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) Larvae

Hasan Tunaz; Mehmet Kubilay Er; Ali Işıkber

doi:10.18016/ksutarimdoga.vi.681089

Araştırma Makalesi

Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) Larvalarında Entomopatojenik Funguslara Karşı Nodülasyon Reaksiyonunda Eikosanoidlerin Rolü

Yıl 2020, Cilt: 23 Sayı: 4, 893 - 897, 31.08.2020

Hasan Tunaz , Mehmet Kubilay Er , Ali Işıkber

https://doi.org/10.18016/ksutarimdoga.vi.681089

Cited By: 1

Öz

Çalışma, iki mantar fungus (Beauveria bassiana 6646 ve Metarhizium anisopliae 3293) karşı, Spodoptera littoralis larvalarındaki nodülasyon reaksiyonları üzerinde eikosanoidlerin rolünü test etmiştir. Larvalar Beauveria bassiana 6646 ve Metarhizium anisopliae 3293 ile enfekte edildiğinde, böcekler bu enfeksiyona karşı nodülasyon reaksiyonu göstermiştir. S. littoralis larvalarına fungus izolatları ile enfekte edilmeden hemen önce eikosanoid biyosentez inhibitörlerinin enjekte edilmesi, fungus izolatlarına nodülasyon tepkisini azaltmıştır. Deksametazon (bir fosfolipaz A2 inhibitörü), indometasin, naproksen, ibuprofen (siklooksijenaz inhibitörleri), esculetin (bir lipooksijenaz inhibitörü) ve fenidon (her ikisi de siklooksijenaz/lipooksijenaz inhibitörü) dahil olmak üzere eikosanoid biyosentezindeki enzim inhibitörleri ile yapılan spesifik uygulamalar. S. littoralis'te fungal enfeksiyonuna karşı nodül oluşumunun azalmasına sebep olmuştur. Bu bulgular, eikosanoidlerin S. littoralis larvalarındaki fungal enfeksiyonlarına karşı nodülasyon reaksiyonlarına dahil olduğu hipotezini desteklemektedir.

Anahtar Kelimeler

Böcek bağışıklığı , Nodülasyon , Entomopatojen fungus , Spodoptera littoralis

Proje Numarası

TÜBİTAK 110O159

Kaynakça

Dunn PE 1986. Biochemical aspects of insect immunology. Annual Review Entomology, 31: 321-339.
Gillespie JP, Kanost MR, Trenczek T 1997. Biological mediators of insect immunity. Annual Review of Entomology, 42: 611-643.
Miller JS, Nguyen T, Stanley-Samuelson DW 1994. Eicosanoids mediate insect nodulation responses to bacterial infections. Proceedings of the National Academy of Sciences USA, 91(26): 12418-12422.
Rosales C 2017. Cellular and Molecular Mechanisms of Insect Immunity. In: Shields,V.D.C. Ed. Insect Physiology and Ecology. InTeach Publicaiton. CCBY. Pp. 179-212.
Russell V, Dunn PE 1996. Antibacterial proteins in the midgut of Manduca sexta during metamorphosis. Journal of Insect Physiolology, 42: 65-71.
SAS Institute Inc. 1989. SAS/STATR User's Guide, Version 6, 4th Ed., vol 2. SAS Institute Inc., Cary, NC.
Satyavathi VV, Minz A, Nagaraju J 2014. Nodulation: An unexplored cellular defense mechanism in insects. Cellular Signalling, 26: 1753-1763.
Stanley DW 2000. Eicosanoids in Invertebrate Signal Transduction Systems. Princeton University Press, Princeton, NJ.
Stanley DW, Kim Y 2014. Eicosanoid Signaling in Insects: from Discovery to Plant. Critical Reviews in Plant Sciences, 33(1):20-63.
Stanley DW, Miller JS 2006. Eicosanoid actions in insect cellular immune functions. Entomologia Experimentalis et Applicata, 119:1-13.
Tunaz H 2006. Influence of Eicosanoids in nodulation reactions against bacteria, Serratia marcescens in larvae of Leptinotarsa decemlineata. KSU Fen Mühendislik Dergisi, 9:159-163.
Tunaz H, Bengin C, Er MK 2008. Nodulation reaction to fungal infections in larvae of Leptinotarsa decemlineata(Say) (Coleoptera: Chrysomelidae) mediated by eicosanoids. Turkish Journal of Agricultural Forestry, 32: 11-18.
Tunaz H, Işıkber AA, Er MK 2018. Blatella germenica'nın erginlerinde iki fungal izolata karşı eikozanoidlerin nodülasyon reaksiyonları üzerine olan fonksiyonu. KSÜ Tarım ve Doğa Dergisi, 21(3): 428-432.

The Role of Eicosanoids in Nodulation Reaction Against Entomopathogenic Fungi in Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) Larvae

Yıl 2020, Cilt: 23 Sayı: 4, 893 - 897, 31.08.2020

Hasan Tunaz , Mehmet Kubilay Er , Ali Işıkber

https://doi.org/10.18016/ksutarimdoga.vi.681089

Cited By: 1

Öz

The study carried out the role of eicosanoids on nodulation reactions in Spodoptera littoralis larvae, against two fungal isolates (Beauveria bassiana 6646 and Metarhizium anisopliae 3293). When the larvae were infected with Beauveria bassiana 6646 and Metarhizium anisopliae 3293, the insects showed a nodulation reaction to this infection. Injecting eicosanoid biosynthesis inhibitors just before the S. littoralis larvae were infected with fungal isolates reduced the nodulation response to fungal isolates. Specific applications, including inhibitors of enzymes in eicosanoid biosynthesis; “dexamethasone (a phospholipase A2 inhibitor), indomethacin, naproxen, ibuprofen (cyclooxygenase inhibitors), esculetin (a lipoxygenase inhibitor) and phenidone (both cyclooxygenase / lipoxygenase inhibitor) reduced” formation of nodules in S. littoralis against fungal infection. These findings support the hypothesis that eicosanoids are involved in nodulation reactions against fungal infections in larvae of S. littoralis.

Anahtar Kelimeler

Insect immunogy , Nodulation , Entomopathogenic fungi , Spodoptera littoralis

Destekleyen Kurum

TÜBİTAK

Proje Numarası

TÜBİTAK 110O159

Teşekkür

This study was supported by TÜBİTAK as project number 110O159.

Kaynakça

Dunn PE 1986. Biochemical aspects of insect immunology. Annual Review Entomology, 31: 321-339.
Gillespie JP, Kanost MR, Trenczek T 1997. Biological mediators of insect immunity. Annual Review of Entomology, 42: 611-643.
Miller JS, Nguyen T, Stanley-Samuelson DW 1994. Eicosanoids mediate insect nodulation responses to bacterial infections. Proceedings of the National Academy of Sciences USA, 91(26): 12418-12422.
Rosales C 2017. Cellular and Molecular Mechanisms of Insect Immunity. In: Shields,V.D.C. Ed. Insect Physiology and Ecology. InTeach Publicaiton. CCBY. Pp. 179-212.
Russell V, Dunn PE 1996. Antibacterial proteins in the midgut of Manduca sexta during metamorphosis. Journal of Insect Physiolology, 42: 65-71.
SAS Institute Inc. 1989. SAS/STATR User's Guide, Version 6, 4th Ed., vol 2. SAS Institute Inc., Cary, NC.
Satyavathi VV, Minz A, Nagaraju J 2014. Nodulation: An unexplored cellular defense mechanism in insects. Cellular Signalling, 26: 1753-1763.
Stanley DW 2000. Eicosanoids in Invertebrate Signal Transduction Systems. Princeton University Press, Princeton, NJ.
Stanley DW, Kim Y 2014. Eicosanoid Signaling in Insects: from Discovery to Plant. Critical Reviews in Plant Sciences, 33(1):20-63.
Stanley DW, Miller JS 2006. Eicosanoid actions in insect cellular immune functions. Entomologia Experimentalis et Applicata, 119:1-13.
Tunaz H 2006. Influence of Eicosanoids in nodulation reactions against bacteria, Serratia marcescens in larvae of Leptinotarsa decemlineata. KSU Fen Mühendislik Dergisi, 9:159-163.
Tunaz H, Bengin C, Er MK 2008. Nodulation reaction to fungal infections in larvae of Leptinotarsa decemlineata(Say) (Coleoptera: Chrysomelidae) mediated by eicosanoids. Turkish Journal of Agricultural Forestry, 32: 11-18.
Tunaz H, Işıkber AA, Er MK 2018. Blatella germenica'nın erginlerinde iki fungal izolata karşı eikozanoidlerin nodülasyon reaksiyonları üzerine olan fonksiyonu. KSÜ Tarım ve Doğa Dergisi, 21(3): 428-432.

Toplam 13 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	İngilizce
Konular	Ziraat, Veterinerlik ve Gıda Bilimleri
Bölüm	ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar	Hasan Tunaz 0000-0003-4942-6056 Mehmet Kubilay Er 0000-0003-1568-8656 Ali Işıkber 0000-0003-1213-3532
Proje Numarası	TÜBİTAK 110O159
Yayımlanma Tarihi	31 Ağustos 2020
Gönderilme Tarihi	28 Ocak 2020
Kabul Tarihi	30 Nisan 2020
Yayımlandığı Sayı	Yıl 2020 Cilt: 23 Sayı: 4

Kaynak Göster

APA	Tunaz, H., Er, M. K., & Işıkber, A. (2020). The Role of Eicosanoids in Nodulation Reaction Against Entomopathogenic Fungi in Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) Larvae. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23(4), 893-897. https://doi.org/10.18016/ksutarimdoga.vi.681089